Recombinant Ag85B vaccine by taking advantage of characteristics of human parainfluenza type 2 virus vector showed Mycobacteria-specific immune responses by intranasal immunization.


:Viral vectors are promising vaccine candidates for eliciting suitable Ag-specific immune response. Since Mycobacterium tuberculosis (Mtb) normally enters hosts via the mucosal surface of the lung, the best defense against Mtb is mucosal vaccines that are capable of inducing both systemic and mucosal immunity. Although Mycobacterium bovis bacille Calmette-Guérin is the only licensed tuberculosis (TB) vaccine, its efficacy against adult pulmonary forms of TB is variable. In this study, we assessed the effectiveness of a novel mucosal TB vaccine using recombinant human parainfluenza type 2 virus (rhPIV2) as a vaccine vector in BALB/c mice. Replication-incompetent rhPIV2 (M gene-eliminated) expressing Ag85B (rhPIV2-Ag85B) was constructed by reverse genetics technology. Intranasal administration of rhPIV2-Ag85B induced Mtb-specific immune responses, and the vaccinated mice showed a substantial reduction in the number of CFU of Mtb in lungs and spleens. Unlike other viral vaccine vectors, the immune responses against Ag85B induced by rhPIV2-Ag85B immunization had an advantage over that against the viral vector. In addition, it was revealed that rhPIV2-Ag85B in itself has an adjuvant activity through the retinoic acid-inducible gene I receptor. These findings provide further evidence for the possibility of rhPIV2-Ag85B as a novel TB vaccine.






Watanabe K,Matsubara A,Kawano M,Mizuno S,Okamura T,Tsujimura Y,Inada H,Nosaka T,Matsuo K,Yasutomi Y




Has Abstract


2014-03-26 00:00:00
















  • Chitosan alters inactivated respiratory syncytial virus vaccine elicited immune responses without affecting lung histopathology in mice.

    abstract::Chitosan is a polysaccharide capable of augmenting immune responses with a proven safety record in animals and humans. These properties make it a potentially attractive agent for the prevention and treatment of infectious disease. Infection by respiratory syncytial virus (RSV) is the leading cause of serious lower res...


    pub_type: 杂志文章


    authors: Muralidharan A,Russell MS,Larocque L,Gravel C,Sauvé S,Chen Z,Li C,Chen W,Cyr T,Rosu-Myles M,Wang L,Li X

    更新日期:2019-07-09 00:00:00

  • Preclinical identification of vaccine induced protective correlates in human leukocyte antigen expressing transgenic mice infected with Coccidioides posadasii.

    abstract::There is an emerging interest to develop human vaccines against medically-important fungal pathogens and a need for a preclinical animal model to assess vaccine efficacies and protective correlates. HLA-DR4 (DRB1∗0401 allele) transgenic mice express a human major histocompatibility complex class II (MHC II) receptor i...


    pub_type: 杂志文章


    authors: Hurtgen BJ,Castro-Lopez N,Jiménez-Alzate MDP,Cole GT,Hung CY

    更新日期:2016-10-17 00:00:00

  • Isolation of antibodies specific to a single conformation-dependant antigenic determinant on the EG95 hydatid vaccine.

    abstract::EG95 is a recombinant vaccine protein that elicits protection against hydatid disease in sheep. Previous studies have shown that the host-protective epitopes on EG95 depend on correct conformation and cannot be represented by simple "linear" peptides. By screening random peptide phage display libraries with polyclonal...


    pub_type: 杂志文章


    authors: Read AJ,Casey JL,Coley AM,Foley M,Gauci CG,Jackson DC,Lightowlers MW

    更新日期:2009-02-11 00:00:00

  • Efficacy of inactivated trivalent influenza vaccine in alleviating the febrile illness of culture-confirmed influenza in children in the 2000-2001 influenza season.

    abstract::During the 2000/2001 influenza season in Japan, children ranging in age from 6 months to 13 years with fever exceeding 37.5 degrees C were recruited. Vaccine efficacy was evaluated by comparing the rates of pre-seasonal vaccination between groups stratified by fever severity. Seven hundred and sixty one patients (33.1...


    pub_type: 杂志文章,多中心研究


    authors: Kamada M,Nagai T,Kumagai T,Igarashi M,Ihara T,Okafuji T,Ochiai H,Sakiyama H,Shimomura K,Suzuki E,Torigoe S,Miyazaki C,Miyata A,Yuri K,Ito Y,Nakayama T,Kase T,Okuno Y

    更新日期:2006-04-24 00:00:00

  • Generation of recombinant pandemic H1N1 influenza virus with the HA cleavable by bromelain and identification of the residues influencing HA bromelain cleavage.

    abstract::The proteolytic enzyme bromelain has been traditionally used to cleave the hemagglutinin (HA) protein at the C-terminus of the HA2 region to release the HA proteins from influenza virions. The bromelain cleaved HA (BHA) has been routinely used as an antigen to generate antiserum that is essential for influenza vaccine...


    pub_type: 杂志文章


    authors: Wang W,Suguitan AL Jr,Zengel J,Chen Z,Jin H

    更新日期:2012-01-20 00:00:00

  • Vaccines and Internet: characteristics of the vaccine safety net web sites and suggested improvements.

    abstract:UNLABELLED:The Internet contains a large amount of useful information on many subjects, but also information of doubtful quality. To help identify Web sites on vaccine safety that fulfil good practice, the Global Advisory Committee on Vaccine Safety of the World Health Organization (WHO) has published criteria to which...


    pub_type: 杂志文章


    authors: Martínez-Mora M,Alvarez-Pasquín MJ,Rodríguez-Salvanés F

    更新日期:2008-12-09 00:00:00

  • Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.

    abstract::Plasmid DNA is a promising vaccine platform that has been shown to be safe and able to be administered repeatedly without vector interference. Enhancing the potency of DNA vaccination through co-delivery of molecular adjuvants is one strategy currently under investigation. Here we describe the use of the novel chemoki...


    pub_type: 杂志文章


    authors: Kraynyak KA,Kutzler MA,Cisper NJ,Khan AS,Draghia-Akli R,Sardesal NY,Lewis MG,Yan J,Weiner DB

    更新日期:2010-02-23 00:00:00

  • A novel lamprey antibody sequence to multimerize and increase the immunogenicity of recombinant viral and bacterial vaccine antigens.

    abstract::Hemagglutinin, the major surface protein of influenza viruses, was recombinantly expressed in eukaryotic cells as a monomer instead of its native trimer, and was only immunogenic when administered with an adjuvant [Pion et al. 2014]. In order to multimerize this antigen to increase its immunogenicity, a cysteine-rich ...


    pub_type: 杂志文章


    authors: Peubez I,Margot S,Buffin S,Pion C,Bernard MC,Dinadayala P,Poncet D,Deloire S,Marco S,Legastelois I

    更新日期:2020-11-25 00:00:00

  • In vitro culture medium influences the vaccine efficacy of Mycobacterium bovis BCG.

    abstract::The varied rates of protection induced by Mycobacterium bovis BCG vaccine against tuberculosis has been attributed to many factors such as genetic variability among BCG strains, rapid clearance of BCG in some populations, and different levels of previous exposure of vaccinated populations to environmental mycobacteria...


    pub_type: 杂志文章


    authors: Venkataswamy MM,Goldberg MF,Baena A,Chan J,Jacobs WR Jr,Porcelli SA

    更新日期:2012-02-01 00:00:00

  • Immunogenicity and protective efficacy of a novel foot-and-mouth disease virus empty-capsid-like particle with improved acid stability.

    abstract::Foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects cloven-hoofed animal species. The FMDV capsid is highly acid labile and viral particles lose their immunogenicity when they disassemble at mildly acidic pHs. The viral capsid of FMDV serotype O is more sensitive th...


    pub_type: 杂志文章


    authors: Xie Y,Li H,Qi X,Ma Y,Yang B,Zhang S,Chang H,Yin X,Li Z

    更新日期:2019-03-28 00:00:00

  • The effect of mass vaccination campaigns against polio on the utilization of routine immunization services: A regression discontinuity design.

    abstract:BACKGROUND:In most low and middle-income countries (LMIC), vaccines are primarily distributed by routine immunization services (RI) at health facilities. Additional opportunities for vaccination are also provided through mass vaccination campaigns, conducted periodically as part of disease-specific initiatives. It is u...


    pub_type: 杂志文章


    authors: Helleringer S,Asuming PO,Abdelwahab J

    更新日期:2016-07-19 00:00:00

  • Induction of mucosal immunity with a replication-defective adenoviral recombinant.

    abstract::A replication-defective adenoviral recombinant expressing the rabies virus glycoprotein was tested for induction of transgene specific central and mucosal antibodies upon systemic inoculation or upon application to the mucosa of the airways, the rectum or the female genital tract. Mice developed serum and mucosal anti...


    pub_type: 杂志文章


    authors: Xiang Z,Ertl HC

    更新日期:1999-04-09 00:00:00

  • Safety of Russian-backbone seasonal trivalent, live-attenuated influenza vaccine in a phase II randomized placebo-controlled clinical trial among children in urban Bangladesh.

    abstract:INTRODUCTION:Live-attenuated influenza vaccines (LAIVs) have the potential to be affordable, effective, and logistically feasible for immunization of children in low-resource settings. MATERIAL AND METHODS:We conducted a phase II, randomized, double-blind, parallel group, placebo-controlled trial on the safety of the ...


    pub_type: 杂志文章,随机对照试验


    authors: Ortiz JR,Goswami D,Lewis KD,Sharmeen AT,Ahmed M,Rahman M,Rahman MZ,Feser J,Neuzil KM,Brooks WA

    更新日期:2015-06-26 00:00:00

  • Immunisation with aluminium-containing vaccine of a child with itching nodule following previous vaccination.

    abstract::Vaccination of children with aluminium sensitisation against diphtheria and tetanus presents a problem, since vaccine without aluminium against these potentially serious infectious diseases is no longer available. This case report presents a 7-month-old boy who, after his second immunisation with DTaP-IPV, developed a...


    pub_type: 杂志文章


    authors: Frederiksen MS,Tofte H

    更新日期:2004-11-15 00:00:00

  • Intradermal immunization improves protective efficacy of a novel TB vaccine candidate.

    abstract::We have developed the Mycobacterium tuberculosis (Mtb) fusion protein (ID83), which contains the three Mtb proteins Rv1813, Rv3620 and Rv2608. We evaluated the immunogenicity and protective efficacy of ID83 in combination with several emulsion-formulated toll-like receptor agonists. The ID83 subunit vaccines containin...


    pub_type: 杂志文章


    authors: Baldwin SL,Bertholet S,Kahn M,Zharkikh I,Ireton GC,Vedvick TS,Reed SG,Coler RN

    更新日期:2009-05-18 00:00:00

  • An experimental vaccine cocktail for Plasmodium falciparum malaria.

    abstract::Surface proteins from several different life-cycle stages of the malaria parasite Plasmodium falciparum were expressed at high levels in the yeast Saccharomyces cerevisiae. Purified proteins, both individually and in cocktails, were used to immunize mice and goats in conjunction with either Freund's adjuvant or a mura...


    pub_type: 杂志文章


    authors: Bathurst IC,Gibson HL,Kansopon J,Hahm BK,Green KM,Chang SP,Hui GS,Siddiqui WA,Inselburg J,Millet P

    更新日期:1993-01-01 00:00:00

  • Effects of a multi-faceted program to increase influenza vaccine uptake among health care workers in nursing homes: A cluster randomised controlled trial.

    abstract::Despite the recommendation of the Dutch association of nursing home physicians (NVVA) to be immunized against influenza, vaccine uptake among HCWs in nursing homes remains unacceptably low. Therefore we conducted a cluster randomised controlled trial among 33 Dutch nursing homes to assess the effects of a systematical...


    pub_type: 杂志文章,随机对照试验


    authors: Looijmans-van den Akker I,van Delden JJ,Verheij TJ,van der Sande MA,van Essen GA,Riphagen-Dalhuisen J,Hulscher ME,Hak E

    更新日期:2010-07-12 00:00:00

  • A pilot study to quantify parental anxiety associated with enrollment of an infant or toddler in a phase III vaccine trial.

    abstract::We sought to measure the anxiety felt by parents at the time of entry into a randomized controlled vaccine trial, and to determine if anxiety level was associated with parental demographic variables or past experience. The children were 2-month-old infants entering a randomized controlled clinical trial (RCT) of a dip...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Langley JM,Halperin SA,Smith B

    更新日期:2003-09-08 00:00:00

  • Effect of 10-valent pneumococcal conjugate vaccine on nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae among children in São Paulo, Brazil.

    abstract::In March 2010, Brazil introduced the 10-valent pneumococcal conjugate vaccine (PCV10) in the routine infant immunization program using a 4-dose schedule and catch-up for children <23months. We investigated PCV10 effect on nasopharyngeal carriage with vaccine-type Streptococcus pneumoniae (Spn) and non-typeable Haemoph...


    pub_type: 杂志文章


    authors: Brandileone MC,Zanella RC,Almeida SCG,Brandao AP,Ribeiro AF,Carvalhanas TMP,Sato H,Andrade AL,Verani JR,Pneumococcal Carriage Study Group.

    更新日期:2016-11-04 00:00:00

  • The outer membrane protein X from Escherichia coli exhibits immune properties.

    abstract::Outer membrane proteins (OMP) are expressed in Gram-negative bacterial cell wall. OmpA from Klebsiella pneumoniae (KpOmpA) has been shown to bind and to activate selectively antigen presenting cells (APCs), eliciting protective CTL responses. In this study, we investigated whether OmpX, another member of the OMP famil...


    pub_type: 杂志文章


    authors: Maisnier-Patin K,Malissard M,Jeannin P,Haeuw JF,Corbière JC,Hoeffel G,Gauchat JF,Nguyen T,Saez JM,Delneste Y

    更新日期:2003-09-08 00:00:00

  • Is there a causal link between hepatitis B vaccination and multiple sclerosis?

    abstract::After the publication of case reports of hepatitis B vaccinees with onset or relapse of multiple sclerosis (MS), followed by a media-driven scare campaign in France, the perception that hepatitis B vaccine causes MS has developed. This has led to a fall in the acceptance of hepatitis B vaccination particularly in Fren...


    pub_type: 杂志文章


    authors: Monteyne P,André FE

    更新日期:2000-04-03 00:00:00

  • Rotavirus infections in a community based cohort in Vellore, India.

    abstract:INTRODUCTION:The burden of infection in communities determines the spread of rotavirus infection and disease in susceptible populations. This study reports rotavirus infection and disease in a community based birth cohort in Vellore. METHODS:Bimonthly surveillance and diarrheal stool were collected from 452 children e...


    pub_type: 杂志文章


    authors: Paul A,Gladstone BP,Mukhopadhya I,Kang G

    更新日期:2014-08-11 00:00:00

  • Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.

    abstract::Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+...


    pub_type: 杂志文章


    authors: Corigliano MG,Fenoy I,Sander V,Maglioco A,Goldman A,Clemente M

    更新日期:2013-12-02 00:00:00

  • Beyond adjuvants: Antagonizing inflammation to enhance vaccine immunity.

    abstract::Since the development of the first vaccine over 200 years ago, vaccines have saved millions of lives and have become the most cost-effective modern medical intervention. However, over 70 years ago, Freund recognized that the effectiveness of the vaccine-induced immune responses could be vastly improved via the co-deli...


    pub_type: 杂志文章,评审


    authors: Alter G,Sekaly RP

    更新日期:2015-06-08 00:00:00

  • Expression of human antibodies in eukaryotic micro-algae.

    abstract::Protein based therapeutics have enjoyed great success over the past decade. Unfortunately, with this clinical success comes a heavy price tag, owing to the inherently high costs of capitalization and production using mammalian cell fermentation. To address this problem, we have begun developing a system for the expres...


    pub_type: 杂志文章,评审


    authors: Mayfield SP,Franklin SE

    更新日期:2005-03-07 00:00:00

  • Delivery of antigen to sialoadhesin or CD163 improves the specific immune response in pigs.

    abstract::Delivery of antigens to antigen presenting cell surface receptors represents a promising strategy to improve immune response to weak immunogenic antigens. We have analyzed the potential of porcine sialoadhesin (Sn) and CD163 as antigen targeting receptors using mouse Igs as surrogate antigens. Sn and CD163 are two end...


    pub_type: 杂志文章


    authors: Poderoso T,Martínez P,Álvarez B,Handler A,Moreno S,Alonso F,Ezquerra Á,Domínguez J,Revilla C

    更新日期:2011-06-24 00:00:00

  • Willingness to participate in Ebola viral disease vaccine trials and receive vaccination by health workers in a tertiary hospital in Ile-Ife, Southwest Nigeria.

    abstract:BACKGROUND:Ebola viral disease (EVD) epidemic need to be contained through means which include vaccination of susceptible population. Vaccination has eradicated major killer diseases. OBJECTIVE:The study determined the health workers willingness to participate in EVD vaccine clinical trials and receive EVD vaccine. M...


    pub_type: 杂志文章


    authors: Olowookere SA,Abioye-Kuteyi EA,Adekanle O

    更新日期:2016-11-11 00:00:00

  • Cytokine production patterns and antibody response to measles vaccine.

    abstract::Cytokines play an important role in the immune response to live measles virus immunization. To gain further insight into the cytokine production profile in response to measles vaccination, we studied interferon-gamma (IFN-gamma), tumor necrosis factor (TNF-alpha), soluble IL-2 receptor (sIL-2R), interleukin-2 (IL-2), ...


    pub_type: 临床试验,杂志文章


    authors: Ovsyannikova IG,Reid KC,Jacobson RM,Oberg AL,Klee GG,Poland GA

    更新日期:2003-09-08 00:00:00

  • Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles.

    abstract::The use of gene guns in ballistically delivering DNA vaccine coated gold micro-particles to skin can potentially damage targeted cells, therefore influencing transfection efficiencies. In this paper, we assess cell death in the viable epidermis by non-invasive near infrared two-photon microscopy following micro-partic...


    pub_type: 杂志文章


    authors: Raju PA,McSloy N,Truong NK,Kendall MA

    更新日期:2006-05-22 00:00:00

  • A canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs.

    abstract::Vaccination is a key element in the control of foot-and-mouth disease (FMD). The majority of the antigenic sites that induce protective immune responses are localized on the FMD virus (FMDV) capsid that is formed by four virus-encoded structural proteins, VP1 to VP4. In the present study, recombinant canine adenovirus...


    pub_type: 杂志文章


    authors: De Vleeschauwer AR,Zhou X,Lefebvre DJ,Garnier A,Watier F,Pignon C,Lacour SA,Zientara S,Bakkali-Kassimi L,De Clercq K,Klonjkowski B

    更新日期:2018-04-12 00:00:00