Expression of proliferating cell nuclear antigen, Ki-67 antigen, estrogen receptor protein, and tumor suppressor p53 gene in cytologic samples of breast cancer: an immunochemical study with clinical, pathobiological, and histologic correlations.

Abstract:

:Sixty-six unselected breast cancers were analyzed in cytologic smears and histologic sections for the expression of Ki-67, proliferating cell nuclear antigen (PCNA), estrogen receptor protein (ERP), and p53 protein using a standard immunochemical method. The results, expressed as both positive cases and labelling index (LI), were compared with clinical and pathobiological variables. Ki-67 and PCNA immunostaining was seen in all cases, whereas ERP was detectable in 46/63 cases and p53 protein in 20/66 cases. The expression of these markers was generally lower in cytology than in histology, though the differences were not statistically significant. PCNA-LI and Ki-67-LI were closely correlated (P < 0.001), the mean PCNA:Ki-67 ratio being 0.92 +/- 0.57. Occasional discrepancies, however, were found. PCNA and Ki-67 expression was associated with an increase in histologic grade and a decrease in ERP content of tumors, whereas p53 was statistically associated with no clinical or pathobiological variables. The data suggest that proliferative activity and oncogene overexpression may be reliably evaluated in breast cancer by FNA cytology, though PCNA is not a suitable indicator for cell proliferation. The results do not resolve the issue as to whether immunostaining for p53 protein constitutes a dedifferentiation product of the tumor, or is a fundamental aspect of the malignant progression. Survival studies in a larger series of tumors are thus needed to elucidate this point.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Pelosi G,Bresaola E,Rodella S,Manfrin E,Piubello Q,Schiavon I,Iannucci A

doi

10.1002/dc.2840110206

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

131-40

issue

2

eissn

8755-1039

issn

1097-0339

journal_volume

11

pub_type

杂志文章
  • Update on human polyomavirus BK nephropathy.

    abstract::Polyomavirus BK (BKV) has ebeen identified as the main cause of polyomavirus-associated nephropathy, a major cause of renal allograft failure. Although BKV-associated nephropathy develops in only 2% to 5% of renal transplant recipients, its prognosis when present is very poor, with irreversible graft failure developin...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21147

    authors: Cimbaluk D,Pitelka L,Kluskens L,Gattuso P

    更新日期:2009-10-01 00:00:00

  • Epidermal growth factor receptor and Kras gene expression: reliability of mutational analysis on cytological samples.

    abstract::Epidermal growth factor receptor (EGFR) and Kras gene mutations are crucial for discriminating patients responsive to anti-EGFR drugs in non-small cell lung cancer (NSCLC) and colorectal cancer (CRC), respectively. The majority of NSCLCs come to clinical attention at an advanced stage when surgery is no longer recomme...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22905

    authors: Bozzetti C,Negri FV,Azzoni C,Naldi N,Nizzoli R,Bortesi B,Zobbi V,Bottarelli L,Tiseo M,Silini EM,Ardizzoni A

    更新日期:2013-07-01 00:00:00

  • How "suspicious" is that nodule? Review of "suspicious" Afirma gene expression classifier in high risk thyroid nodules.

    abstract:BACKGROUND:The Afirma gene expression classifier (GEC) is being increasingly utilized to confirm the benign nature of indeterminate FNA cytology results thus avoiding unnecessary surgical procedures. However the "suspicious" result of the Afirma GEC does not classify these indeterminate nodules further in determining a...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23680

    authors: Roychoudhury S,Klein M,Souza F,Gimenez C,Laser A,Shaheen Cocker R,Chau K,Das K

    更新日期:2017-04-01 00:00:00

  • Blastic plasmacytoid dendritic cell neoplasm with a history of cytopenia: A case report.

    abstract::Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive hematologic malignancy arising from plasmacytoid dendritic cell precursors. The disease typically manifests in the skin, but it also evolves into a leukemic phase or can be complicated by other myeloid malignancies, especially myelomonocytic...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24463

    authors: Yan M,Wang W,Cen X,Wang L,Sun Y,Wang B,Ou J,Nong L,Ren H,Zhu P,Wang M

    更新日期:2020-05-06 00:00:00

  • Aspiration cytology of neuroblastoma: light microscopy with transmission and scanning electron microscopic correlations.

    abstract::Fine-needle aspiration biopsies from three patients with neuroblastoma were studied by light microscopy, and the morphologic findings were correlated with those from transmission and scanning electron microscopy. Light microscopic examination of the aspiration smears from all three cases revealed small and large round...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840040410

    authors: Akhtar M,Ali MA,Sackey K,Sabbah R,Bakry M

    更新日期:1988-01-01 00:00:00

  • Immunocytochemical typification of mesothelial cells in effusions: in vivo and in vitro models.

    abstract::We have performed immunocytochemical, immunoelectron microscopy, Western blot, and culture techniques using monoclonal antibodies against cytokeratin, vimentin, and desmin on 17 benign and 20 malignant effusions of pleural and ascitic origin. Triple coexpression of these three antigens was observed in benign reactive ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840100313

    authors: Ferrandez-Izquierdo A,Navarro-Fos S,Gonzalez-Devesa M,Gil-Benso R,Llombart-Bosch A

    更新日期:1994-01-01 00:00:00

  • A metastatic renal carcinoid tumor presenting as breast mass: a diagnostic dilemma.

    abstract::We present clinicopathological and cytological findings of a well-defined breast mass in a patient with history of primary renal carcinoid tumor. Fine-needle aspiration (FNA) cytology showed monotonous tumor cells with plasmacytoid appearance arranged singly and in small clusters. Occasional tumor cells were arranged ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20631

    authors: Hasteh F,Pu R,Michael CW

    更新日期:2007-05-01 00:00:00

  • Granulomatous prostatitis: a spectrum including nonspecific, infectious, and spindle cell lesions.

    abstract::Most granulomas of the prostate are nonspecific; infectious, post-operative, and allergic lesions are much less common. Fine-needle aspiration findings in the typical case are distinctive and easily recognized. Several series have been reported, but few have included histologic follow-up. We describe three cases of gr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070513

    authors: Stanley MW,Horwitz CA,Sharer W,Uke E,Kaye K,Burton L

    更新日期:1991-01-01 00:00:00

  • Chondroid syringoma diagnosed by fine needle aspiration cytology.

    abstract::Chondroid syringoma is a rare benign skin adnexal tumor of eccrine/apocrine origin affecting commonly the head and neck region. It used to be previously called as mixed tumor of skin because of the presence of both the epithelial and mesenchymal components. There are only few case reports describing the fine needle as...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21159

    authors: Kumar B

    更新日期:2010-01-01 00:00:00

  • Chlamydia/gonorrhea combo and HR HPV DNA testing in liquid-based pap.

    abstract::Residual material from liquid-based pap (LBP) test has been used for molecular testing of Human Papilloma Virus (HR HPV) DNA. LBP test specimens (1,025) were evaluated to determine how often residual material was insufficient to perform microbiological testing. The specimens were tested for Chlamydia trachomatis and N...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20325

    authors: Hawthorne CM,Farber PJ,Bibbo M

    更新日期:2005-09-01 00:00:00

  • Fine-needle aspiration cytology of clear-cell sarcoma of the kidney: study of eight cases.

    abstract::The largest series, to date, of fine-needle aspiration cytology (FNAC) findings in clear-cell sarcoma of the kidney (CCSK) is presented. All fine-needle aspirates of pediatric renal masses over a 17-yr period were reviewed. Eight out of 119 aspirates from late-stage childhood renal tumors (6.72%) were found to be CCSK...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20317

    authors: Iyer VK,Agarwala S,Verma K

    更新日期:2005-08-01 00:00:00

  • Pseudoglandular schwannoma mimicking alveolar rhabdomyosarcoma: A diagnostic challenge on fine-needle cytology.

    abstract::Pseudoglandular schwannoma (PGS) is a rare morphological variant of benign schwannoma. PGS is quite distinct from the somewhat better characterized glandular schwannoma variant. PGS is characterized by the presence of gland-like structures lined with pseudocolumnar or cuboidal-like neoplastic Schwann cells lining vari...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24345

    authors: Yaprak Bayrak B,Vural Ç,Paksoy N

    更新日期:2020-03-01 00:00:00

  • Use of the Thin Prep Pap Test in clinical practice.

    abstract::The Thin Prep Pap Test (Cytyc Corp., Boxborough, MA) received approval by the Food and Drug Administration in May 1996 as an alternative to the traditional conventional smear. The present direct-to-vial study assessed the utility of thin-layer technology for cervicovaginal screening in clinical practice. From May 1997...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199902)20:2<70::aid-dc5>3.

    authors: Guidos BJ,Selvaggi SM

    更新日期:1999-02-01 00:00:00

  • Corpora amylacea in pleural effusion.

    abstract::Corpora amylacea are predominantly found in the brain, prostate, and lung. Recent characterizations of their components suggest an important role in protection and clearing. We report the presence of corpora amylacea in pleural effusion in a patient with lupus. The differential diagnoses and potential significance are...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24684

    authors: Mani H,Wang BG

    更新日期:2020-12-21 00:00:00

  • Does the 'equal management of equal risks' model cause overtreatment in patients with positive cervical cytology results for ASCUS/non-HPV16/18 oncogenic types?

    abstract:OBJECTIVES:According to the American Society of Colposcopy and Cervical Pathology (ASCCP), cervical cytology patients who test positive for atypical squamous cells of undetermined significance (ASCUS) and the human papilloma virus (HPV) positive are recommended to undergo colposcopies. This study compared the colposcop...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24029

    authors: Karaca İ,Öztürk M,Comba C,Demirayak G,Erdoğan ŞV,Hoşgören M,Özdemir İA

    更新日期:2019-02-01 00:00:00

  • Polyoma virus infection is a prominent risk factor for bladder carcinoma in immunocompetent individuals.

    abstract::Despite various reports of BK viral (BKV) DNA sequences or proteins in tumors of the urogenital tract, there has been no study statistically linking infection by this polyoma virus (PV) to tumor development. All PV are potential transforming viruses, the large T-antigen of which interacts with tumor suppressor protein...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20429

    authors: Weinreb DB,Desman GT,Amolat-Apiado MJ,Burstein DE,Godbold JH Jr,Johnson EM

    更新日期:2006-03-01 00:00:00

  • Reproducibility of atypia of undetermined significance/follicular lesion of undetermined significance category using the bethesda system for reporting thyroid cytology when reviewing slides from different institutions: A study of interobserver variability

    abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) offers a six-tiered diagnostic scheme for thyroid Fine Needle Aspiration (FNA): Benign, Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance (AUS/FLUS), suspicious for follicular neoplasm, suspicious for malignancy...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23681

    authors: Padmanabhan V,Marshall CB,Akdas Barkan G,Ghofrani M,Laser A,Tolgay Ocal I,David Sturgis C,Souers R,Kurtycz DF

    更新日期:2017-05-01 00:00:00

  • Cytomorphologic features of metastatic urothelial carcinoma in serous effusions.

    abstract::Metastatic urothelial carcinoma (UC) to serous effusion (SE) is extremely rare and its cytomorphological features have only been described in case reports. In this study, we searched the pathology database at University of Michigan for SEs due to metastatic UC in the last 20 years. A total of 25 cases from 20 patients...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22896

    authors: Huang CC,Attele A,Michael CW

    更新日期:2013-07-01 00:00:00

  • Cerebrospinal Fluid Cytological Diagnosis in Multiple Myeloma With Leptomeningeal Involvement: A Report of Two Cases.

    abstract::Multiple myeloma (MM) with central nervous system (CNS) infiltration is uncommon and the diagnosis is more complicated than that of MM. Here we report two cases of CNS MM that was diagnosed by cerebrospinal fluid cytology examination. Cerebrospinal fluid cytology examination can help to detect malignant cells and immu...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23600

    authors: Ren H,Zou Y,Zhao Y,Li J,Han X,He J,Guan H

    更新日期:2017-01-01 00:00:00

  • Endoscopic ultrasound-guided fine needle aspiration with liquid-based cytology preparation in the diagnosis of metastatic small-cell carcinoma in the pancreas.

    abstract::Pancreatic metastasis is extremely rare, particularly from small-cell lung cancer (SCLC). Studies on the role of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) with liquid-based cytology (LBC) in the diagnosis of metastatic small-cell carcinoma in the pancreas have been rarely conducted. We report herei...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23990

    authors: Son JH,Park HK,Kim HS,Kim NH,Kim JW,Bae WK,Kim KA,Lee JS,Lee YS

    更新日期:2018-11-01 00:00:00

  • Hürthle cell metaplasia in chronic lymphocytic thyroiditis: Role of age factor and review of literature on its molecular pathogenesis.

    abstract:OBJECTIVES:Hürthle cell (HC) metaplasia is an important feature of chronic lymphocytic (Hashimoto) thyroiditis (HT). However, it is rarely observed in juvenile form HT. In a study based on fine needle aspiration cytology, the cytomorphological difference between pediatric HT and its adult form was studied, with special...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.24140

    authors: Das DK

    更新日期:2019-05-01 00:00:00

  • Metastatic tonsillar squamous cell carcinoma masquerading as a pancreatic cystic tumor and diagnosed by EUS-guided FNA.

    abstract::Metastatic carcinoma to the pancreas is uncommon and head and neck squamous carcinoma metastatic to the pancreas is extremely rare. Metastatic squamous cell carcinoma to the pancreas presents a unique diagnostic challenge: in addition to mimicking the rare primary squamous cell carcinoma of the pancreas based on cytol...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23769

    authors: Glass R,Andrawes SA,Hamele-Bena D,Tong GX

    更新日期:2017-11-01 00:00:00

  • Fine-needle aspiration biopsy of metastatic small cell carcinoma from extrapulmonary sites.

    abstract::Like a pulmonary counterpart, extrapulmonary small cell carcinoma (SCC) is an aggressive tumor with a high rate of metastasis. Forty-nine fine-needle aspiration biopsies (FNABs) (36 patients) of various primary sites other than the lung diagnosed as metastatic SCC (including Merkel cell carcinoma) were reviewed. FNABs...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199809)19:3<177::aid-dc4>3

    authors: Shin HJ,Caraway NP

    更新日期:1998-09-01 00:00:00

  • Concordance between thyroid nodule sizes measured by ultrasound and gross pathology examination: effect on patient management.

    abstract::Ultrasound examination (US) is an essential tool in the evaluation of thyroid nodules. The size determined by US is used to distinguish between clinical vs. nonclinical thyroid nodules i.e. greater than or equal to or less than 1 cm. In this study, we evaluated the concordance between the sizes of thyroid nodules meas...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20714

    authors: Deveci MS,Deveci G,LiVolsi VA,Gupta PK,Baloch ZW

    更新日期:2007-09-01 00:00:00

  • Clinical, imaging, and cytopathological features of solid pseudopapillary tumor of the pancreas: a clinicopathologic study of three cases and review of the literature.

    abstract::Solid pseudopapillary tumors are rare pancreatic neoplasms of uncertain pathogenesis that rarely metastasize and usually occur in young women. We describe the clinical, imaging, and cytopathological features of solid pseudopapillary tumor of the pancreas. We reviewed the clinical presentation, imaging, morphologic/imm...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20365

    authors: Bhanot P,Nealon WH,Walser EM,Bhutani MS,Tang WW,Logroño R

    更新日期:2005-12-01 00:00:00

  • Fine-needle aspiration of cystic nephroma (multilocular cyst of the kidney).

    abstract::Cystic nephromas are rare tumors of the kidney most commonly affecting boys or adult females. The fine-needle aspiration cytomorphology has not yet been described. A renal cystic mass in a 56 year old female was aspirated under ultrasound guidance. Papanicolaou stained smears of the cyst fluid revealed markedly atypic...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080408

    authors: Clark SP,Kung IT,Tang SK

    更新日期:1992-01-01 00:00:00

  • Unusual presentation of granulocytic sarcoma in the breast: a case report and review of the literature.

    abstract::This is a case of granulocytic sarcoma presenting as bilateral breast masses in a 40-yr-old woman with concurrent unsuspected chronic myeloid leukemia diagnosed by fine-needle aspiration. The granulocytic differentiation was recognized on Diff-Quik-stained cytology smears and confirmed rapidly on flow cytometry on the...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0339(200101)24:1<53::aid-dc1009>3.0.c

    authors: Ngu IW,Sinclair EC,Greenaway S,Greenberg ML

    更新日期:2001-01-01 00:00:00

  • Significance of incidental detection of filariasis on aspiration smears: a case series.

    abstract::Filariasis is a major public health problem in tropical and subtropical countries including India. Although there are reports of incidentally diagnosed cases of lymphatic filariasis in the existing literature, the significance of this finding needs to be summarised in one place. The association of filariasis with neop...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21264

    authors: Gupta S,Gupta R,Bansal B,Singh S,Gupta K,Kudesia M

    更新日期:2010-07-01 00:00:00

  • Characteristic cytologic findings of epithelioid hemangioendothelioma: A case report and review of literature.

    abstract::Epithelioid hemangioendothelioma is a rare vascular neoplasm of low-grade malignancy. We observed a case of epithelioid hemangioendothelioma arising in a cervical lymph node. Fine needle aspiration cytology showed several characteristic findings of epithelioid hemangioendothelioma, that is, nuclear inclusion bodies, n...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21377

    authors: Kurisu Y,Tsuji M,Kuwabara H,Shibayama Y

    更新日期:2011-02-01 00:00:00

  • PREPmate automated processor: comparison of automated and manual methods of liquid-based gynecologic sample preparation.

    abstract::The development of procedures for fully automated processing of liquid-based gynecologic samples has been the focus of considerable interest to the cytology laboratory. Liquid-based collection and processing technology has been shown to improve sample adequacy, resulting in an overall improvement in quality of sample ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10193

    authors: Rowe LR,Marshall CJ,Bentz JS

    更新日期:2002-11-01 00:00:00