Abstract:
:Research in hepatocellular gene therapy requires a consistently reproducible cell marker to detect transplanted hepatocytes. We have used a Y-specific genomic DNA probe to accomplish this goal. This technique enables the identification of transplanted male cells in recipient female tissues. Donor hepatocytes from male mice were transplanted into female mice via splenic injection. Recipient mouse livers were harvested 1, 24, and 48 hr after transplant. Transplanted (male) hepatocytes were detected in liver biopsy sections using in situ hybridization with the Y chromosome probe.
journal_name
Transplantationjournal_title
Transplantationauthors
Eckert JW,Buerkle CJ,Major AM,Finegold MJ,Brandt MLdoi
10.1097/00007890-199501150-00019subject
Has Abstractpub_date
1995-01-15 00:00:00pages
109-11issue
1eissn
0041-1337issn
1534-6080journal_volume
59pub_type
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