Abstract:
:A quantitative analysis of pectolytic enzymes (polygalacturonase (PG), pectin methyl esterase (PME) and six isoenzymes of pectate lyase (PL)) produced byErwinia bacteria in the presence of diverse carbon sources was made by preparative electrophoresis. Synthesis of each of these enzymes was regulated independently; different induction and repression ratios (about 10- to 1000-fold) were observed for diverse PL isoenzymes, PG and PME. The possibility of using specially constructed media for the production of pectinase complexes with a specific spectra of pectolytic enzymes has been demonstrated.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Shevchik VE,Evtushenkov AN,Babitskaya HV,Fomichev YKdoi
10.1007/BF01195828subject
Has Abstractpub_date
1992-03-01 00:00:00pages
115-20issue
2eissn
0959-3993issn
1573-0972journal_volume
8pub_type
杂志文章abstract::A rapid, simple and sensitive method for detection of ferulic and p-coumaric acids using HPLC has been developed which can be used to determine the respective phenolic acid esterase activities of microorganisms. Prior concentration, purification or derivatization of the samples are not required. As little as 0.5 mg fe...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00360920
更新日期:1996-05-01 00:00:00
abstract::Shikimic acid (SA) is the key synthetic material of Oseltamivir, which is an effective drug for the prevention and treatment of influenza. In this study, to block the downstream metabolic pathway of SA, the shikimate kinase isoenzyme genes aroK and aroL were deleted by Red recombination. Moreover, the key enzyme genes...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1679-z
更新日期:2014-09-01 00:00:00
abstract::Oxalic acid plays major role in the pathogenesis by Sclerotinia sclerotiorum; it lowers the pH of nearby environment and creates the favorable condition for the infection. In this study we examined the degradation of oxalic acid through oxalate oxidase and biocontrol of Sclerotinia sclerotiorum. A survey was conducted...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1130-2
更新日期:2012-11-01 00:00:00
abstract::Bacteria associated with eight field-collected and five cultured soft corals of Briareum sp., Sinularia sp., Sarcophyton sp., Nephtheidae sp., and Lobophytum sp. were screened for their abilities in producing antimicrobial metabolites. Field-collected coral samples were collected from Nanwan Bay in southern Taiwan. Cu...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1138-7
更新日期:2012-12-01 00:00:00
abstract::The search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. A yeast strain isolated from sugarcane juice and identified as Meyerozyma guilliermondii was evaluated for the ability to ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-016-2036-1
更新日期:2016-05-01 00:00:00
abstract::The potential of three Serratia marcescens strains (CFFSUR-B2, CFFSUR-B3 and CFFSUR-B4) isolated from tropical regions in Mexico to inhibit the mycelial growth and conidial germination of Colletotrichum gloeosporioides, causal agent of fruit anthracnose, was evaluated. The ability of these strains to produce prodigios...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0803-6
更新日期:2012-01-01 00:00:00
abstract::The potential use of Sporosarcina pasteurii in possible biotechnological applications on a large scale (ground improvement, consolidation of building structures and ornamental stone, or in developing bio-materials for the building industry), is based on its ability to produce high amounts of carbonate in a short perio...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1844-z
更新日期:2015-06-01 00:00:00
abstract::Of three molecular forms of polygalacturonases synthesized by Aspergillus alliaceus on glucose media, two were exopolygalacturonases (exoPG1 and exoPG2) and one was an endopolygalacturonase (endoPG). Low-methoxylated beet pectin was the preferred substrate for the endoPG and exoPG2 whereas pectic acid was the optimal ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367111
更新日期:1995-05-01 00:00:00
abstract::The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichme...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1179-y
更新日期:2013-02-01 00:00:00
abstract::The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemic...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2429-4
更新日期:2018-03-08 00:00:00
abstract::Production of biogas in batch digesters at 30°C from sheep droppings produced 93 l gas/kg dry matter whereas cattle dung yielded 234 l/kg dry matter. When the sheep droppings and cattle dung were used at 25:75 (w/w), gas production per kg dry matter was equal to that of cattle dung only. The methane content of the bio...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00327830
更新日期:1993-03-01 00:00:00
abstract::The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutan...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1573-0
更新日期:2014-05-01 00:00:00
abstract::Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the y...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-017-2259-9
更新日期:2017-05-01 00:00:00
abstract::Lactic acid bacteria (LAB) isolated from different sources (dairy products, fruits, fresh and fermented vegetables, fermented cereals) were screened for antimicrobial activity against other bacteria, including potential pathogens and food spoiling bacteria. Six strains have been shown to produce bacteriocins: Lactococ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1671-7
更新日期:2014-09-01 00:00:00
abstract::The use of indigenous bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. The isolation and selection of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for site decontamination. Two different media, a minimal me...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1277-5
更新日期:2013-06-01 00:00:00
abstract::A strain of Aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. Sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. The optimum pH for activity and stability were 5.5 and 6.5, respectively. Its optimum temperature ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00329399
更新日期:1991-05-01 00:00:00
abstract::An inhibitory factor (IF) produced byAspergillus niger strain 19, and which inhibits the action of glucoamylase on starch, has the ability to be tightly adsorbed on to various raw starches, though the amount differs from starch to starch. Based on the hydrolysis of the IF-starch complex by glucoamylase, the inhibition...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01202122
更新日期:1990-12-01 00:00:00
abstract::Shewanella colwelliana, a marine bacterium isolated in association with the oyster Crassostrea virginica, produces an abundant exopolysaccharide with potential commercial value as an adhesive under aqueous conditions. Its utilization of glucose was modulated by stoichiometric concentrations of yeast extract. In Brain ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367663
更新日期:1994-09-01 00:00:00
abstract::This study was conducted to report the richness of endophytic Penicillium and Talaromyces species isolated from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest (Caatinga), to verify their ability to produce the enzyme L-asparaginase and to partially optimise the production of biomass...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2547-z
更新日期:2018-10-28 00:00:00
abstract::Brucellae are Gram-negative intracellular bacterial pathogens that infect humans and animals, bringing great economic burdens to developing countries. Live attenuated Brucella vaccines (strain M5-90 or others) are the most efficient means for prevention and control of animal brucellosis. However, these vaccines have s...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-016-2015-6
更新日期:2016-04-01 00:00:00
abstract::In natural environments, microorganisms form microbial aggregates called biofilms able to adhere to a multitude of different surfaces. Yeasts make no exception to this rule, being able to form biofilms in a plethora of environmental niches. In food realms, yeast biofilms may cause major problems due to their alterativ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-020-02911-5
更新日期:2020-08-10 00:00:00
abstract::In order to study the mechanisms regulating the phenanthrene degradation pathway and the intermediate-metabolite accumulation in strain S. paucimobilis 20006FA, we sequenced the genome and compared the genome-based predictions to experimental proteomic analyses. Physiological studies indicated that the degradation inv...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-017-2391-6
更新日期:2017-12-06 00:00:00
abstract::Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 2...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1338-9
更新日期:2013-10-01 00:00:00
abstract::Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Micro...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0858-4
更新日期:2012-02-01 00:00:00
abstract::Hyperthermophiles are characterized by a temperature optimum for growth between 80 and 110°C. They are considered to represent the most ancient phenotype of living organisms and thus their metabolic design might reflect the situation at an early stage of evolution. Their modes of metabolism are diverse and include che...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00339135
更新日期:1995-01-01 00:00:00
abstract::Aquilaria malaccensis produces agarwood in response to wounding and fungal attack. However, information is limited regarding Aquilaria's interaction with its diverse fungal community. In this study, time-related changes of three natural fungal colonizers in two wounded wild A. malaccensis were tracked, beginning a few...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1668-2
更新日期:2014-09-01 00:00:00
abstract::5-Enopyruvylshikimate-3-phosphate synthase (EPSP synthase) is an important enzyme in the shikimate pathway mediating the biosynthesis of aromatic compounds in plants and microorganisms. A novel class II EPSP synthase AroA S. fredii from Sinorhizobium fredii NGR234 was overexpressed in Escherichia coli BL21. It was pur...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1724-y
更新日期:2014-11-01 00:00:00
abstract::Cystic Fibrosis is a genetic disease and it affects the respiratory and digestive systems. Pseudomonas aeruginosa infections in Cystic Fibrosis are presented as the main cause for high mortality and morbidity rates. Pseudomonas aeruginosa populations can regulate their virulence gene expressions via the bacterial comm...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-016-2105-5
更新日期:2016-09-01 00:00:00
abstract::Endophytes are recognised as potential sources of novel secondary metabolites, including enzymes and drugs, with applications in medicine, agriculture and industry. There is a growing need for new enzymes, including proteases, for use in industry that can function under a variety of conditions. In this study, three fu...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1598-z
更新日期:2014-06-01 00:00:00
abstract::Pseudomonas aeruginosa possesses an arcade of both cell-associated and extracellular cytotoxic virulence factors which are regulated by a multi-component quorum sensing system. Many research studies report success of lactonase in combating the pathogenicity of P. aeruginosa but delivery of lactonase remains a challeng...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1645-9
更新日期:2014-08-01 00:00:00