Immunoelectron microscopic localization of prostatic-specific antigen in human prostate by the protein A-gold complex.

Abstract:

:Human prostate specimens from 25 patients provided 15 normal, four benign prostatic hypertrophy (BPH), and seven adenocarcinoma samples which were studied using a polyclonal antibody against prostatic specific antigen (PSA) and protein A-gold complex. Our study showed the presence of gold particles in cytoplasmic vesicles and granules, rough endoplasmic reticulum, and occasional lysosomal dense bodies in columnar or cuboidal cells of acini from normal, BPH and well-differentiated cancerous specimens, but not in the acinar basal cells. Some moderately differentiated and most poorly differentiated tumors contained undifferentiated neoplastic cells in which PSA localizations often were associated with membranous structures since specific cytoplasmic organelles were not differentiated. In prostatic stroma, invasive cells which were of differentiated type localized gold particles. In addition, some neutrophils and macrophages also localized PSA suggesting their role in phagocytosis of extracellularly released PSA in the stroma of BPH and cancerous samples. Thus far, the authors have not observed neutrophils and macrophages with gold particles in normal prostate. We suggest that neutrophils and macrophages were involved in transport of some extracellularly released PSA from the prostatic stroma or other metastatic sites to sera in BPH and cancer patients.

journal_name

Cancer

journal_title

Cancer

authors

Sinha AA,Wilson MJ,Gleason DF

doi

10.1002/1097-0142(19870915)60:6<1288::aid-cncr2820

subject

Has Abstract

pub_date

1987-09-15 00:00:00

pages

1288-93

issue

6

eissn

0008-543X

issn

1097-0142

journal_volume

60

pub_type

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