Production of alkaline protease by a new Bacillus subtilis isolate for use as a bating enzyme in leather treatment.

Abstract:

:Bacillus subtilis, isolated from tannery waste, produced an alkaline protease at optimal activity when grown in a casein/gelatine medium in a stirred tank fermenter at 37°C with the dissolved oxygen tension at 40% air saturation. Optimum protease activity (223 U ml(-1)) was at pH 8.5 and was stable for 1 h up to 45°C but at 60°C lost 80% activity. Use of the crude protease as a bating agent for producing high quality leather is indicated. Tensile strength, bursting strength, tear strength and elongation at break of prepared leather were increased with increasing amounts of protease used for bating.

authors

Hameed A,Natt MA,Evans CS

doi

10.1007/BF00360930

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

289-91

issue

3

eissn

0959-3993

issn

1573-0972

journal_volume

12

pub_type

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