Detection of interferon-alpha expression by PCR in patients with chronic hepatitis C and hepatitis non-A, non-B.

Abstract:

:The polymerase chain reaction (PCR) was used to examine expression of interferon-alpha (IFN A) genes in general and the expression of messenger RNA (mRNA) encoding the subtypes IFN-alpha-2 and IFN-alpha-4 in blood and liver biopsy samples from patients with chronic hepatitis C or hepatitis non-A, non-B (HC/HNANB) infection entered into a trial of IFN-alpha-2a therapy. Peripheral blood mononuclear cells (PBMC) from healthy controls and HC/HNANB infected patients were studied for their capacity to produce transcripts encoding IFN-alpha after stimulation with Sendai virus. Expression at the level of mRNA for IFN A and the subtypes IFN A2 and A4 was detected in both controls and HC/HNANB infected patients PBMC and no significant difference was seen in expression of IFN A transcripts or level of total IFN-alpha secreted into culture supernatants between controls and patients. Interferon A, and specifically IFN A2 and IFN A4 transcripts were detected in a high proportion of liver biopsies from patients with HC/HNANB infection. The presence of IFN A mRNA (and specifically IFN A2 and IFN A4) showed no correlation to histological improvement nor response to therapy. The use of PCR to detect those IFN A genes that are not expressed, thereby identifying subtypes that may be lacking, could be the key to the choice of IFN-alpha subtypes that are used for effective therapy.

authors

Brandt ER,Devenish RJ,Hertzog PJ,Dudley FJ,Linnane AW

doi

10.1111/j.1440-1746.1994.tb01258.x

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

373-80

issue

4

eissn

0815-9319

issn

1440-1746

journal_volume

9

pub_type

临床试验,杂志文章,多中心研究,随机对照试验
  • Comparative study of diagnostic value of cytologic sampling by endoscopic ultrasonography-guided fine-needle aspiration and that by endoscopic retrograde pancreatography for the management of pancreatic mass without biliary stricture.

    abstract:BACKGROUND:Endoscopic ultrasonography-guided fine-needle aspiration (EUS-FNA) can now provide a cytopathological diagnosis of underlying pancreatic malignancy with higher success rates than endoscopic retrograde pancreatography (ERP). To determine the significance of EUS-FNA for the diagnosis of pancreatic mass without...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2005.03900.x

    authors: Wakatsuki T,Irisawa A,Bhutani MS,Hikichi T,Shibukawa G,Takagi T,Yamamoto G,Takahashi Y,Yamada Y,Watanabe K,Obara K,Suzuki T,Sato Y

    更新日期:2005-11-01 00:00:00

  • Results of sofosbuvir-based combination therapy for chronic hepatitis C cohort of Indian patients in real-life clinical practice.

    abstract:BACKGROUND AND AIM:The introduction of sofosbuvir has revolutionized the treatment of chronic hepatitis C. This study was planned to observe whether the efficacy and tolerability of sofosbuvir-based regimens demonstrated in phase 3 clinical trial results translate into real-life clinical practice. METHODS:This prospec...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 临床试验,杂志文章

    doi:10.1111/jgh.13628

    authors: Sood A,Midha V,Mahajan R,Narang V,Mehta V,Wander P,Sharma S,Kaur K,Singh D

    更新日期:2017-04-01 00:00:00

  • Sustained virologic response achieved after curative treatment of hepatitis C virus-related hepatocellular carcinoma as an independent prognostic factor.

    abstract:BACKGROUND AND AIM:Whether an antiviral interferon (IFN)-based therapy (IBT) after curative treatment of hepatocellular carcinoma (HCC) improves the prognosis in patients with hepatitis C virus (HCV)-related HCC remains to be elucidated. METHODS:A total of 178 patients within the Milan criteria underwent curative trea...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.12925

    authors: Kanogawa N,Ogasawara S,Chiba T,Saito T,Motoyama T,Suzuki E,Ooka Y,Tawada A,Kanda T,Mikami S,Azemoto R,Kaiho T,Shinozaki M,Ohtsuka M,Miyazaki M,Yokosuka O

    更新日期:2015-07-01 00:00:00

  • Spectrum of histopathologic findings in patients with achalasia reflects different etiologies.

    abstract:BACKGROUND:The etiology of achalasia is still unknown. The aim of the present study was to elucidate its underlying pathologies and their chronology by investigation of esophageal specimens in patients undergoing surgery (esophageal resection or myotomy) for achalasia. METHODS:In 17 patients with achalasia, histopatho...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2006.04250.x

    authors: Gockel I,Bohl JR,Doostkam S,Eckardt VF,Junginger T

    更新日期:2006-04-01 00:00:00

  • Incidence and risk factors of Pneumocystis jirovecii pneumonia in Korean patients with inflammatory bowel disease.

    abstract:BACKGROUND AND AIM:Little is known whether routine prophylaxis against Pneumocystis jirovecii pneumonia (PJP) is needed in patients with inflammatory bowel disease (IBD) on immunosuppression, especially in Asian populations. We, therefore, sought to investigate the incidence and risk factors of PJP in patients with IBD...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14832

    authors: Nam K,Park SH,Lee J,Jo S,Kim SO,Noh S,Park JC,Kim JY,Kim J,Ham NS,Oh EH,Song EM,Hwang SW,Yang DH,Ye BD,Byeon JS,Myung SJ,Yang SK

    更新日期:2020-02-01 00:00:00

  • Case report: acanthosis nigricans in association with primary biliary cirrhosis: resolution after liver transplantation.

    abstract::A case is described of a 58 year old Caucasian male with primary biliary cirrhosis (PBC) who first presented with acanthosis nigricans of both axillae, skin pigmentation, which was pronounced over the posterior surface of the neck, and generalized pruritus. Following orthotopic liver transplantation for progressive li...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1996.tb00025.x

    authors: Pham TH,Kaushik S,Lin BP,Jones DB

    更新日期:1996-11-01 00:00:00

  • KRAS mutation analysis by next-generation sequencing in endoscopic ultrasound-guided sampling for solid liver masses.

    abstract:BACKGROUND AND AIM:KRAS mutation analysis may provide ancillary diagnostic yield in an endoscopic ultrasound (EUS)-guided histopathological evaluation. We evaluated the clinical impact of KRAS mutation analysis with cytological and histological evaluations in EUS-guided tissue sampling, using a core biopsy needle for s...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.13423

    authors: Choi HJ,Moon JH,Kim HK,Lee YN,Lee TH,Cha SW,Cho YD,Park SH

    更新日期:2017-01-01 00:00:00

  • Factors that influence cecal intubation rate during colonoscopy in deeply sedated patients.

    abstract:BACKGROUND AND AIM:The technical performance of colonoscopy performed in deeply sedated patients differs from that performed without sedation or under minimal to moderate sedation. The aim of this study is to evaluate the factors affecting cecal intubation during colonoscopy performed under deep sedation. METHODS:A to...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2011.06795.x

    authors: Hsu CM,Lin WP,Su MY,Chiu CT,Ho YP,Chen PC

    更新日期:2012-01-01 00:00:00

  • Homing of the bone marrow-derived interstitial cells of Cajal is decreased in diabetic mouse intestine.

    abstract:BACKGROUND:Interstitial cells of Cajal (ICCs), which express c-Kit receptor tyrosine kinase (KIT), play an important role in gastrointestinal motility. Loss of ICCs likely contributes to diabetic gastrointestinal motility disorder, however, the mechanism of attrition remains unknown. Here, we test the hypothesis that t...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2011.06670.x

    authors: Li Y,Kojima H,Fujino K,Matsumura K,Katagi M,Urabe H,Chan L,Eguchi Y,Zhao L,Kimura H

    更新日期:2011-06-01 00:00:00

  • Trend of colorectal cancer in Hong Kong: 1983-2006.

    abstract:BACKGROUND AND AIM:The incidence of colorectal cancer (CC) is increasing in many Asian countries, but decreasing in western countries. The present study examined the local incidence of CC in the past few decades. METHODS:A population based study, using data from Hong Kong (HK) Cancer Registry, was carried out to exami...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2009.06130.x

    authors: Yee YK,Gu Q,Hung I,Tan VP,Chan P,Hsu A,Pang R,Lam CS,Wong BC

    更新日期:2010-05-01 00:00:00

  • Haemodynamic effects of enalaprilat on portal hypertension in patients with HBsAg-positive cirrhosis.

    abstract::It has been suggested that enalaprilat inhibits the renin-angiotensin-aldosterone system in plasma and tissue; it may therefore reduce portal vascular pressure owing to secondary hyperaldosteronism in patients with liver cirrhosis. In order to evaluate this concept, 20 patients with hepatitis B surface antigen (HBsAg)...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1440-1746.1995.tb01090.x

    authors: Chiang HT,Cheng JS,Lin M,Tseng WS,Chang JM,Lai KH

    更新日期:1995-05-01 00:00:00

  • Gastrointestinal manifestations of leukemia.

    abstract::Gastrointestinal (GI) manifestations of leukemia occur in up to 25% of patients at autopsy, generally during relapse. Its presence varies with the type of leukemia and has been decreasing over time due to improved chemotherapy. Gross leukemic lesions are most common in the stomach, ileum, and proximal colon. Leukemia ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1746.2011.06908.x

    authors: Ebert EC,Hagspiel KD

    更新日期:2012-03-01 00:00:00

  • Overlap in disease concept of functional esophageal disorders and minor esophageal motility disorders.

    abstract:BACKGROUND AND AIM:Functional gastrointestinal disorders are the most common disorders in gastroenterology and are currently considered as gut-brain interaction disorders with multiple related factors including motility disturbance. However, high-resolution manometry (HRM) had revealed a new disease concept known as mi...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14691

    authors: Sato H,Takahashi K,Mizuno KI,Hashimoto S,Kawata Y,Mizusawa T,Tominaga K,Yokoyama J,Terai S

    更新日期:2019-11-01 00:00:00

  • miR-873-5p inhibits the progression of colon cancer via repression of tumor suppressor candidate 3/AKT signaling.

    abstract:BACKGROUND AND AIM:We previously discovered that tumor suppressor candidate 3 (TUSC3) was overexpressed and predicted worse prognosis in colon cancer patients. However, the mechanisms of upregulation of TUSC3 in colon cancer remained unclear. METHODS:MiR-873-5p was predicted and identified as the regulator of TUSC3 vi...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14697

    authors: Zhu Y,Zhang X,Qi M,Zhang Y,Ding F

    更新日期:2019-12-01 00:00:00

  • Molecular adsorbent recirculating system: albumin dialysis-based extracorporeal liver assist device.

    abstract::Extracorporeal liver assist devices have been used for more than five decades to support patients with liver failure. Numerous modifications have been made to both biological as well as mechanical liver assist devices. Possibly, an ideal liver assist device would be one that would perform optimal detoxification and sy...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1746.17.s3.14.x

    authors: Kapoor D

    更新日期:2002-12-01 00:00:00

  • Adefovir dipivoxil therapy in liver transplant recipients for recurrence of hepatitis B virus infection despite lamivudine plus hepatitis B immunoglobulin prophylaxis.

    abstract:BACKGROUND:Treatment of post-transplantation recurrence of hepatitis B virus (HBV) infection despite prophylaxis with hepatitis B immunoglobulin (HBIG) and lamivudine combination therapy is not easy. Because HBV reinfection has a severe course and could result in graft failure in liver transplant recipients, prompt med...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2006.04609.x

    authors: Akyildiz M,Karasu Z,Zeytunlu M,Aydin U,Ozacar T,Kilic M

    更新日期:2007-12-01 00:00:00

  • The additive effect of opioids and nitric oxide in increasing pentylenetetrazole-induced seizure threshold in cholestatic mice.

    abstract:BACKGROUND:Accumulation of endogenous opioids and overproduction of nitric oxide has been reported in cholestatic mice. It is well known that endogenous opioids and nitric oxide alter the susceptibility of experimental animals to different models of seizure. METHODS:The alterations in clonic seizure thresholds, induce...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1746.2002.02658.x

    authors: Homayoun H,Sayyah M,Dehpour AR

    更新日期:2002-01-01 00:00:00

  • Prevalence and risk of colorectal adenoma in asymptomatic Koreans aged 40-49 years undergoing screening colonoscopy.

    abstract:BACKGROUND AND AIM:Colorectal cancer screening is recommended for average-risk persons beginning at age 50. However, information about the incidence and risk factors of precursor adenoma in preceding decades is limited. The aim of this study was to determine the prevalence and risk factors of colorectal adenoma in pers...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2009.06147.x

    authors: Chung SJ,Kim YS,Yang SY,Song JH,Park MJ,Kim JS,Jung HC,Song IS

    更新日期:2010-03-01 00:00:00

  • Anorectal physiology and transit in patients with disorders of thyroid metabolism.

    abstract:BACKGROUND:Data on anorectal physiology in patients with disordered thyroid metabolism are lacking. This prospective study was performed to evaluate anorectal physiology in patients with either hyperthyroidism and diarrhoea, or hypothyroidism and constipation in order to assess slow transit in hypothyroid patients. ME...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1746.1999.01865.x

    authors: Deen KI,Seneviratne SL,de Silva HJ

    更新日期:1999-04-01 00:00:00

  • The prognostic factors and trajectory of HRQOL in patients with pancreatic cancer who received psychiatric intervention.

    abstract:BACKGROUND AND AIM:Patients with advanced pancreatic cancer have severe pain, anxiety, and depression, and these symptoms deteriorate quality of life (QOL). Previous study reported that early psychiatric intervention for advanced cancer patients may improve QOL. We evaluated the trajectory of health-related QOL (HRQOL)...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.13172

    authors: Sugimoto H,Kawashima H,Ohno E,Hayashi D,Kuwahara T,Morishima T,Kawai M,Suhara H,Takeyama T,Yamamura T,Funasaka K,Nakamura M,Miyahara R,Watanabe O,Ishigami M,Suzuki M,Kimura H,Ozaki N,Hashimoto S,Goto H,Hirooka Y

    更新日期:2016-03-01 00:00:00

  • Functional and phenotypical activation of leucocytes in inflamed human colonic mucosa.

    abstract::Infiltrating leucocytes are activated to generate reactive oxygen species or to produce several molecules in inflamed colonic mucosa. To clarify the phenotypical and functional properties of activating cells in colitic mucosa, 23 patients with ulcerative colitis and 13 controls were studied using a combined method for...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1997.tb00376.x

    authors: Oshitani N,Sawa Y,Hara J,Adachi K,Nakamura S,Matsumoto T,Arakawa T,Kuroki T

    更新日期:1997-12-01 00:00:00

  • Epidemiology of alcoholic and nonalcoholic fatty liver disease in China.

    abstract::The prevalence of patients presenting with fatty liver disease (FLD) in China has approximately doubled over the past two decades. At present, FLD, which is typically diagnosed by imaging, is highly prevalent (≈ 27% urban population) in China and is mainly related to obesity and metabolic syndrome (MetS). However, the...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1111/jgh.12036

    authors: Fan JG

    更新日期:2013-08-01 00:00:00

  • High incidence of zinc deficiency among Filipino children with compensated and decompensated liver disease.

    abstract:BACKGROUND AND AIM:The role of zinc in the nutrition and growth of children with chronic liver disease is poorly defined. The present study determined the serum zinc levels of children with compensated liver disease (CLD) and decompensated liver disease (DLD) and compared this with healthy children. Zinc levels were al...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2009.06003.x

    authors: Umusig-Quitain P,Gregorio GV

    更新日期:2010-02-01 00:00:00

  • Aberrant expression of epidermal growth factor receptor and its interaction with protein kinase C δ in inflammation associated neoplastic transformation of human esophageal epithelium in high risk populations.

    abstract:BACKGROUND AND AIM:Esophageal cancer is the second most common cancer among Indian males and is mostly associated with tobacco smoking and alcohol consumption. Epidermal growth factor receptor (EGFR) is a member of Type I tyrosine kinases. Its activation causes the docking of various proteins in its cytosolic tail. In ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2010.06526.x

    authors: Parthasarathy S,Dhayaparan D,Jayanthi V,Devaraj SN,Devaraj H

    更新日期:2011-02-01 00:00:00

  • Risk of liver cirrhosis and hepatocellular carcinoma in subjects with hepatitis B and delta virus infection: a study from Kure, Japan.

    abstract::To investigate the effect of hepatitis delta virus (HDV) superinfection on the long-term outcome of Japanese subjects with chronic hepatitis B virus (HBV) infection, we examined the presence of antibodies to hepatitis delta antigen (anti-HD) in serial serum samples collected from 1127 subjects with chronic HBV infecti...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1993.tb01543.x

    authors: Tamura I,Kurimura O,Koda T,Ichimura H,Katayama S,Kurimura T,Inaba Y

    更新日期:1993-09-01 00:00:00

  • Presence of spur cells as a highly predictive factor of mortality in patients with cirrhosis.

    abstract:BACKGROUND AND AIM:The presence of spur-cell anemia (SCA) is due to lipid disturbances of the erythrocyte membrane and may develop in patients with advanced liver cirrhosis. The accurate predicting value of SC for survival has not been clarified. The aim of this study was to evaluate SCA as a prognostic indicator in pa...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.12473

    authors: Alexopoulou A,Vasilieva L,Kanellopoulou T,Pouriki S,Soultati A,Dourakis SP

    更新日期:2014-04-01 00:00:00

  • Relationship between dietary patterns and non-alcoholic fatty liver disease: A systematic review and meta-analysis.

    abstract:BACKGROUND AND AIM:Non-alcoholic fatty liver disease (NAFLD) is one of the most common causes of chronic liver disease. Previous studies have investigated the association between dietary patterns and NAFLD, but their results are contradictory. Therefore, we carried out this meta-analysis to examine the association betw...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.15363

    authors: Hassani Zadeh S,Mansoori A,Hosseinzadeh M

    更新日期:2020-12-02 00:00:00

  • Seroepidemiological study on hepatitis delta virus infection in the Irabu Islands, Okinawa, Japan.

    abstract::A seroepidemiological study was performed to clarify the prevalence of hepatitis delta virus (HDV) infection among the general population in the Irabu islands, Okinawa, Japan. Of 2028 healthy people examined who had received their annual health check-up in 1994-95, 195 (9.6%) were positive for hepatitis B surface anti...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1997.tb00425.x

    authors: Sakugawa H,Nakasone H,Shokita H,Kawakami Y,Nakachi N,Adaniya H,Mizushima T,Nakayoshi T,Kinjo F,Saito A,Taira M,Takaesu H,Onga N

    更新日期:1997-04-01 00:00:00

  • Ethnicity is a strong predictor for Helicobacter pylori infection in young women in a multi-ethnic European city.

    abstract:BACKGROUND AND AIM:At the same time that Helicobacter pylori prevalence is declining in Western countries, immigrants from developing countries with high H. pylori prevalence have settled in Western urban areas. Actual epidemiological data on H. pylori in a migrant community may help in realizing a more selective appro...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.12315

    authors: den Hollander WJ,Holster IL,den Hoed CM,van Deurzen F,van Vuuren AJ,Jaddoe VW,Hofman A,Perez Perez GI,Blaser MJ,Moll HA,Kuipers EJ

    更新日期:2013-11-01 00:00:00

  • Echo-Doppler measurements of portal vein and superior mesenteric artery blood flow in humans: inter- and intra-observer short-term reproducibility.

    abstract::The reproducibility of echo-Doppler measurements of portal vein and superior mesenteric artery blood flow has not been extensively studied. In the present study, two groups of subjects were examined to test inter- and intra-observer reproducibility. Each study population consisted of 15 nonportal hypertensive and 15 p...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1996.tb00008.x

    authors: Iwao T,Toyonaga A,Shigemori H,Oho K,Sumino M,Sato M,Tanikawa K

    更新日期:1996-01-01 00:00:00