Abstract:
:A systematic workflow consisting of comparative genomics, metabolic pathways analysis and additional drug prioritization parameters identified 264 proteins of Vibrio cholerae which were predicted to be absent in Homo sapiens. Among these, 40 proteins were identified as essential proteins that could serve as potential drug and vaccine targets. Additional prioritization parameters characterized 11 proteins as vaccine candidates while druggability of each of the identified proteins as evaluated by the Drug Bank database which prioritized 16 proteins suitable for drug targets. As a case study, we built a homology model of one of the potential drug targets, MurA ligase, using MODELLER (9v12) software. The model has been further explored for in silico docking with inhibitors having druggability potential from the Drug Bank database. Results from this study could facilitate selecting V. cholerae proteins for drug design and vaccine production pipelines in future.
journal_name
Genomicsjournal_title
Genomicsauthors
Chawley P,Samal HB,Prava J,Suar M,Mahapatra RKdoi
10.1016/j.ygeno.2013.12.002subject
Has Abstractpub_date
2014-01-01 00:00:00pages
83-93issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(13)00227-9journal_volume
103pub_type
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