[Cell culture model for hepatocyte culture in bioreactors for metabolic utilization in hybrid liver support system].

Abstract:

:Hybrid systems with hepatocyte cultures in bioreactors are in development for therapeutical liver assistance. Here, a culture model with a special bioreactor construction was developed: Capillary membrane systems create a three dimensional artificial framework for hepatocyte adhesion, aggregation and reorganization of tissue structures. Many of small parallel capillary membrane units perfuse a few hepatocytes. Different capillary materials enable different functions. Cell perfusion between independent plasma inflow and outflow capillaries, independent oxygen supply and carbon dioxide removal as well as a co- culture with sinusoidal endothelial cells are possible. An in vitro study with bioreactors (n = 9), containing 2.5 x 10(9) pig hepatocytes was performed, measuring external metabolism of the cells: cytochrome P450-activity (midazolam metabolism, lidocaine/MEGX-test), synthesis (albumin), liver function test (galactose elimination) and cell alteration (LDH, GOT, GLDH, GPT, gamma GT) were investigated. The results demonstrate that external function of primary hepatocytes can be maintained over a period of at least three weeks.

journal_name

Zentralbl Chir

authors

Gerlach J,Encke J,Müller C,Neuhaus P

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

334-40

issue

5

eissn

0044-409X

issn

1438-9592

journal_volume

119

pub_type

杂志文章
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    doi:

    authors: Hansen O,Graupe F,Schwenk W,Stock W

    更新日期:1996-01-01 00:00:00

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    pub_type: 杂志文章

    doi:

    authors: Schneider JH,Lepsien G

    更新日期:1988-01-01 00:00:00

  • Evaluation of toupet antireflux operation, by means of gastroesophageal scintiscan.

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    doi:

    authors: Vara-Thorbeck R,Morales OI,Guerrero JA,Ruiz-Morales M,Salvi M,Vara C

    更新日期:1989-01-01 00:00:00

  • [Surgical therapy of acute pancreatitis].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Pahlig H,Göbel U

    更新日期:1988-01-01 00:00:00

  • [Hereditary pancreatitis - a clinically relevant cause of pancreatic adenocarcinoma?].

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    更新日期:2001-11-01 00:00:00

  • [Validity of Recommendations for Secondary Prevention of Colon Cancer].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章,评审

    doi:10.1055/s-0032-1328074

    authors: Porzsolt F,Ittner D,Henne-Bruns D

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  • [Minimal incision abdominal aortic aneurysm repair].

    abstract:OBJECTIVE:The use of a limited incision for abdominal aortic aneurysm (AAA) repair was evaluated, and its outcome was analyzed in comparison to standard open repair. PATIENTS AND METHODS:Between February 2000 and August 2001, 20 patients with an AAA underwent minimal incision repair (MINI) for tube graft implantation....

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    doi:10.1055/s-2002-33948

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    更新日期:2002-09-01 00:00:00

  • [Postoperative coma caused by liver failure].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Kremer B,Henne-Bruns D

    更新日期:1986-01-01 00:00:00

  • [Liver rupture in peliosis hepatis].

    abstract::A 44-year old woman had pain in the epigastric region under the thorax aperture on the left side 6 weeks prior to admission. Her doctor had prescribed Rewodina and Myocuran without success. Then she suffered circulatory collapse twice. Upon hospitalization, she experienced colicky upper abdominal pain and vomiting....

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Adam G,Lesser T,Neumann R

    更新日期:1991-01-01 00:00:00

  • [Situation-adjusted Treatment of Haemorrhoidal Disease].

    abstract::In addition to the stage several factors influence the treatment of haemorrhoids. The adequate treatment of haemorrhoids is not solely dependent on the stage, thus a situation-adapted therapy should be preferred. Advantages, disadvantages and specific characteristics of different therapy strategies in addition to pote...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章,评审

    doi:10.1055/s-0032-1328183

    authors: Kersting S,Berg E

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  • [Breast carcinoma in pregnancy. Clinical, histological and immunohistochemical findings].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Meden H,Marx D,Rath W,Tsikuras P,Kuhn W,Schauer A

    更新日期:1992-01-01 00:00:00

  • [Surgery of proctologic diseases in the polyclinic].

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    pub_type: 杂志文章

    doi:

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    更新日期:1988-01-01 00:00:00

  • [New Applications and Indications for Contrast-Enhanced Sonography in Endovascular Aortic Repair].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章,评审

    doi:10.1055/s-0035-1546147

    authors: Zimmermann H,Reiser M,Meimarakis G,Paprottka P,Clevert DA

    更新日期:2015-10-01 00:00:00

  • [Plastic reconstructive options for chronic pilonidal disease].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Stremmel W

    更新日期:1980-01-01 00:00:00

  • [Laparoscopic cholecystectomy. A recommendable indication in acute cholecystitis?].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章,评审

    doi:

    authors: Manger T,Fahlke J,Pross M,Fuhlroth J,Röhl FW,Lippert H

    更新日期:1999-01-01 00:00:00

  • [Unusual complications of the Peutz-Jeghers-syndrome in two consecutive generations of the same family].

    abstract::The Peutz-Jeghers syndrome is an autosomal dominant inherited disease, characterized by the presence of hamartomatous polyposis of the gastrointestinal tract and perioral mucocutaneous pigmentation. The incidence of surgical complications in these patients is relatively rare, and correlates with the size and location ...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:10.1055/s-2002-22026

    authors: Lazaridis Ch,Papaziogas B,Atmatzidis K,Kalaitzis E,Pavlidis T,Papaziogas T

    更新日期:2002-02-01 00:00:00

  • [Ulcerative colitis-associated colorectal carcinoma. DNA ploidy as indicator of impending malignant transformation?].

    abstract::The onset of a malignant transformation in long-standing ulcerative colitis is difficult to predict. The value of the clinical and histomorphological parameters in current use is limited. It was thus aim of the present study to investigate the value of DNA-ploidy for the early detection of a malignant transformation i...

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    pub_type: 杂志文章

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  • [Osteosynthesis of ipsilateral hip and femoral shaft fractures with the PFN-long].

    abstract:AIM:The paper retrospectively evaluates a set of patients with ipsilateral fractures of the proximal femur and shaft treated with PFN-long. MATERIAL:19 patients (14 males and 5 females), aged 48 in average, were treated from 1998-2003. The fractures included 2 types: combined intraarticular femoral neck and shaft frac...

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    doi:10.1055/s-2005-836368

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  • [Laparoscopic therapy of colorectal cancer--wishful thinking or realism?].

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  • [Ileus of the large intestine as a late sequela of direct diaphragmatic rupture].

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    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Schütter FW,Kiroff P,Leister I

    更新日期:1995-01-01 00:00:00

  • [Explorative laparotomy and splenectomy for staging Hodgkin's disease in children (author's transl)].

    abstract::We recommend explorative laparotomy with splenectomy in the field of Hodgkin's lymphoma even in childhood. Operation is an early therapeutic and diagnostic method enabling further treatment to be made in relation to the stages of the disease. A chance of better survival seems to exist in children with Hodgkin's diseas...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Blau HJ

    更新日期:1977-01-01 00:00:00

  • [The Whipple partial duodenopancreatectomy--its value in the treatment of chronic pancreatitis].

    abstract::The surgical treatment of chronic pancreatitis is indicated only in the complicated disease. The aim is mainly the treatment of pain, of mechanical obstacles and to exclude suspicion of cancer. Between October 1972 and January 1993 713 patients with chronic pancreatitis were treated at the Surgical University Hospital...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Saeger HD,Schwall G,Trede M

    更新日期:1995-01-01 00:00:00

  • [Prospective multicenter trial of gastric cancer surgery--a contribution to clinical research on quality control].

    abstract::By the mean of a prospective multicenter observational study (East German Gastric Cancer Study - EGGCS), 1 139 consecutive patients with gastric cancer were enrolled in 80 East German surgical departments from January 1 to December 31, 2002. Out of them, 1,031 (90.5%) underwent surgical intervention. The resection rat...

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    pub_type: 杂志文章,多中心研究

    doi:10.1055/s-2005-836491

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    更新日期:2005-04-01 00:00:00

  • [Documentation and therapeutic standards in anesthesia and intensive care medicine--means for quality assurance and cost control].

    abstract::Patient data management in anaesthesia and intensive care should include besides medical data of individual patients economically important parameters, e.g. working time or cost of material. Integration of this data management system in the hospital information network enables case-oriented analyses for costs in relat...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章,评审

    doi:

    authors: Bach A,Martin E

    更新日期:1996-01-01 00:00:00

  • [The modern surgery of face lift: indications and results].

    abstract::In this retrospective study with a group of 55 patients aged 47.7 +/- 8.3 years, surveyed for a period of 16 months, the results of different face lift techniques--subcutaneous face lift combined with imbrication of the SMAS-Platysma complex versus sub-SMAS-dissection with transposition of the SMAS-Platysma complex--w...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Hönig JF

    更新日期:2000-01-01 00:00:00

  • [Laparoscopic total gastrectomy with extended lymphadenectomy and intracorporal circular stapled esophagojejunostomy].

    abstract::Laparoscopic total gastrectomy for early and advanced gastric cancer is an exacting procedure which is increasingly performed in specialised institutions. Not only gastric resection and extended lymphadenectomy but especially the reconstruction by oesophagojejunostomy is a technically demanding and vulnerable operativ...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:10.1055/s-0033-1360122

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    更新日期:2014-02-01 00:00:00

  • [Advantages of lymph node staging in 872 stomach carcinoma patients].

    abstract::The German Stomach Cancer TNM Study Group registered 1444 patients from 1982 to 1984 in a multicenter observational study to validate the tumor node metastasis stage groupings. The follow-up-rate in 1989 was 99.4%. Among others the pattern of lymph node metastases and the benefit of systematic lymphadenectomy (SLA) wa...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Keller E

    更新日期:1996-01-01 00:00:00

  • [Effectiveness of nephroprotection by the selection of contrast media used during vascular interventions in patients with chronic renal failure?].

    abstract:BACKGROUND:The increasing number of endovascular procedures made aware of a kidney disease induced by contrast media (CM). Contrast-induced nephropathy (= CIN) can develop in 0.6-44 % of the treated patients by angiography and / or endovascular intervention. The incidence in high-risk patients ranges from 50 to 70 %. I...

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    pub_type: 杂志文章

    doi:10.1055/s-0031-1283759

    authors: Schönefeld E,Höwler S,Osada N,Torsello G

    更新日期:2011-10-01 00:00:00

  • [Acute upper gastrointestinal hemorrhage caused by ruptured aneurysm of the right gastroepiploic artery].

    abstract::Visceral aneurysms are very rare (0.1% in autopsy-statistics). In our own review of literature we have found only 9 described cases of an aneurysm of the gastroepiploic artery. In the presented case an aneurysm was found when the patient developed an acute upper gastrointestinal bleeding. Due to the high risk of ruptu...

    journal_title:Zentralblatt fur Chirurgie

    pub_type: 杂志文章

    doi:

    authors: Jakschik J,Decker D,Vogel H,Hirner A

    更新日期:1993-01-01 00:00:00