Abstract:
:We have recently shown that a single intrathymic injection of synthetic 25mer peptides, representing full sequences of the hypervariable domain of RT1.BuB (4 peptides) and RT1.Du beta (4 peptides) WF class II MHC molecules, 48 hr before transplantation induces donor-specific unresponsiveness to WF rat renal allografts in adult LEW recipients. The induction of unresponsiveness was abrogated by the recipient's thymectomy within the first week after intrathymic injection. Peripheral T cells of long-term survivors exhibited antigen-specific hyporesponsiveness in the LEW x WF MLR. Studies on the mechanisms of induction of acquired thymic unresponsiveness to alloantigen in vivo and in vitro are now reported. First, since we have previously demonstrated in LEW responders that only 4 of the 8 synthetic 25mer peptides, 2 RT1.Du beta and 2 RT1.Bu beta sequences, were immunogenic in vitro and in vivo, we compared the tolerogenicity of the immunogenic versus the nonimmunogenic peptides. While LEW rats intrathymically injected with the nonimmunogenic peptides acutely rejected their renal allografts within 6-10 days, animals injected with the immunogenic peptides did not reject their grafts and are surviving > 100 days with normal allograft function. In vitro studies established that peripheral T cells from intrathymically tolerized animals exhibited antigen-specific hyporesponsiveness in the LEW x WF MLR starting as early as 1 week posttransplant. Immunohistological evaluation of renal allografts from intrathymically tolerized animals 1 week postengraftment showed marked reduction in mononuclear cell infiltrates with no evidence of tubulitis, and marked reduction in intragraft staining for activation and inflammatory cytokines and alloantibodies, as compared with acutely rejecting controls. Systemic administration of 1000 U of rIL-2 daily for 5 days starting on the day of transplantation abrogated the tolerogenic effect of intrathymic MHC allopeptides. Injection of 100 micrograms of a single immunogenic peptide, RT1.Du beta 2 (residues 20-44), into the thymus of responder LEW rats 48 hrs before immunization with RT1.Du beta 2 effected significant reduction of in vitro proliferation of primed lymphocytes to RT1.Du beta 2, an effect that was abrogated by addition of rIL-2 in vitro. In contrast, thymectomy beyond 2 weeks and administration of rIL-2 at 4-6 weeks after transplantation failed to cause rejection. These observations indicate that thymic recognition of immunodominant class II MHC allopeptides leads to peripheral T cell anergy that mediates the induction phase of systemic unresponsiveness to renal allografts. The maintenance phase appears to be mediated by dense anergy or clonal deletion.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Transplantationjournal_title
Transplantationauthors
Sayegh MH,Perico N,Gallon L,Imberti O,Hancock WW,Remuzzi G,Carpenter CBsubject
Has Abstractpub_date
1994-07-27 00:00:00pages
125-32issue
2eissn
0041-1337issn
1534-6080journal_volume
58pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:The long-term outcome of lung transplantation is impeded by the development of obliterative bronchiolitis (OB). We sought to investigate the relationship between the apoptosis of tracheal epithelial cells and bone marrow-derived myofibroblasts in the process of OB. METHODS:The mouse orthotopic tracheal tran...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001230
更新日期:2016-09-01 00:00:00
abstract::Nonadherence to immunosuppressants in renal transplant recipients is a major factor affecting graft survival, but it is difficult to detect accurately in clinical practice. Adherence was measured in 153 adult renal transplant recipients using self-report questionnaires and interview, clinician rating, and cyclosporine...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.tp.0000110412.20050.36
更新日期:2004-03-15 00:00:00
abstract:BACKGROUND:Osteoarthritis is the most prevalent joint disease and a frequent cause of joint pain, functional loss, and disability. Osteoarthritis often becomes chronic, and conventional treatments have demonstrated only modest clinical benefits without lesion reversal. Cell-based therapies have shown encouraging result...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318291a2da
更新日期:2013-06-27 00:00:00
abstract:BACKGROUND:Conflicting data have been reported about the effect of Toxoplasma serostatus on mortality after heart transplantation. Either a positive or a negative recipient Toxoplasma serostatus was found to be associated with increased mortality. METHODS:We evaluated the effects of T. gondii infection on survival of ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182a9274a
更新日期:2013-12-27 00:00:00
abstract:BACKGROUND:In France, foreign patients, whether resident or not in France, can register on the national waiting list under certain conditions. We compared waiting time to kidney transplantation, the level of HLA matching and graft cold ischemia time between foreign patients and French patients living in mainland France...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000205182.96861.3a
更新日期:2006-04-27 00:00:00
abstract::A mouse monoclonal antibody (CIA) produced against human Ia antigens that reacts with 20-30% of human peripheral blood lymphocytes was found to react with more than 90% of dog lymphocytes. Less than 41% of the Ia-positive dog lymphocytes expressed surface immunoglobulins. Immunoprecipitation studies with 125I-labeled ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198308000-00016
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:Malnutrition is common in patients with end-stage liver disease considered for transplantation, but it is unclear whether this affects the outcome after transplantation. AIM.: To determine whether the severity of malnutrition in liver transplant candidates affects outcome after transplantation. METHODS:We d...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000150022.64564.c2
更新日期:2005-02-15 00:00:00
abstract::The properties of rat liver dendritic cells (DC) were analyzed after collagenase digestion of the tissue and enrichment with density gradient centrifugation. The liver macrophages (Kupffer cells) were eliminated by adherence before the gradient centrifugation. The morphology of isolated DC in May-Grunwald-Giemsa (MGG)...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198805000-00019
更新日期:1988-05-01 00:00:00
abstract::CD18 antibodies react with the common beta chain of the human leukocyte function antigen (LFA1)* and thus block the functions mediated by the three identified molecules in humans. A murine CD18 monoclonal antibody was infused in 8 leukemic patients receiving allogeneic T-depleted bone marrow transplantation in order t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198903000-00014
更新日期:1989-03-01 00:00:00
abstract::Dendritic cell (DC) progenitors were propagated in liquid culture from nonparenchymal cells resident in normal mouse (B10.BR; H-2k, I-E+) liver in response to granulocyte-macrophage colony stimulating factor (GM-CSF). The liver-derived DC progenitors were MHC class II-/dim and did not express counter receptors for CTL...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199502270-00019
更新日期:1995-02-27 00:00:00
abstract::This study compares two recently introduced radioimmunoassay kits involving specific monoclonal antibodies to cyclosporine. One kit (Sandimmun) involved 3H-labeled CsA (3H-CsA) as tracer and the other (CY-CLO-Trac-SP) involved a 125I-labeled conjugated derivative of CsA. The kits were nearly equivalent in method perfo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198904000-00019
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:In organ transplantation, several immunosuppressants are currently used to control graft rejection. Clinically, no single immunosuppressive agent can completely prevent posttransplantation immunoreaction; thus, combination therapy is usually performed. Novel agents with more powerful immunosuppressive activi...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000144312.08782.16
更新日期:2004-12-15 00:00:00
abstract:BACKGROUND:Carbon monoxide (CO) inhalation protects organ by reducing inflammation and cell death during transplantation processes in animal model. However, using CO in clinical transplantation is difficult due to its delivery in a controlled manner. A manganese-containing CO releasing molecules (CORM)-401 has recently...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002201
更新日期:2018-07-01 00:00:00
abstract::Liver transplantation is the most effective treatment for selected patients with hepatocellular carcinoma. However, cancer recurrence, posttransplantation, remains to be the critical issue that affects the long-term outcome of hepatocellular carcinoma recipients. In addition to tumor biology itself, increasing evidenc...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000001844
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:After transplantation, donor dendritic cells (DC) migrating to the draining lymph node of the recipient are thought to play an important role in the initiation of graft rejection. In this study, we compared the in vivo migration of DC after allogeneic skin transplantation with that after congeneic skin trans...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199903270-00008
更新日期:1999-03-27 00:00:00
abstract::Heterologous anti-immunoglobulin (AI) is a potent immunosuppressive agent which compares favorably to anti-thymocyte serum (ATS) in inhibiting E and EAC-rosette formation and diminishing the secondary antibody response to sheep erythrocytes and E. coli lipopolysaccharide. AI prolongs H-2 incompatible skin allograft su...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197609000-00003
更新日期:1976-09-01 00:00:00
abstract::Graft and patient survival rates after transplantation of ABO-incompatible liver allografts have been poor. We used plasmapheresis and a potent immunosuppressive regimen to control hemagglutinin levels and prevent early rejection. Ten patients who had a United Network for Organ Sharing status of 4 received ABO-incompa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199504150-00011
更新日期:1995-04-15 00:00:00
abstract::Healthy rat islets were encapsulated in alginate-polylysine-alginate capsules measuring 0.25-0.35 mm in diameter using a modified encapsulation technique. The encapsulated islets were transplanted intraperitoneally in nonimmunosuppressed streptozotocin-induced diabetic BALB/c mice. The diabetic condition of the experi...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199206000-00002
更新日期:1992-06-01 00:00:00
abstract::A prospective controlled study was carried out in 60 consecutive cadaver renal donors comparing cold storage to pulsatile machine-perfusion preservation. Each donor served as its own control, by allocating one of the kidneys to each of the two preservation methods. There were 51 evaluable pairs of kidneys. Recipient a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199008000-00011
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:Liver transplant recipients suffer many postoperative complications. Few studies evaluated the effects of fluid management on these complications. We conducted an observational cohort study to evaluate the association between intraoperative fluid balance and postoperative acute kidney injury (AKI) and other ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002998
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Oral ingestion of potassium dichromate produces a complex spectrum of complications. It has an extremely poor prognosis and usually leads to rapid death. METHODS:We report the case of a 16-year-old male patient who was admitted to hospital after oral ingestion of potassium dichromate with suicidal intention...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200006150-00044
更新日期:2000-06-15 00:00:00
abstract::Cyclosporine prevents the development of diabetes in spontaneously diabetic BB rats and NOD mice. However, islet transplants have been shown to be subject to immunologic destruction in hosts treated with CsA and anti-CD4 antibody or those rendered tolerant to donor antigens. This study determines whether the minimum d...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199005000-00001
更新日期:1990-05-01 00:00:00
abstract:BACKGROUND:To stimulate organ donation, an organ procurement organization (OPO)-wide effort was undertaken to increase donors after cardiac death (DCD) over a 5-year period. This included commonality of protocols, pulsatile perfusion of kidneys, centralization of data and a regional allocation variance designed to mini...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000179641.82031.ea
更新日期:2006-05-27 00:00:00
abstract::This is a short review of tolerance from the point of view of the clinician. Various examples of tolerance occurring in patients and animal models that relate to the clinical experience are described. There may be different mechanisms by which tolerance is achieved, but from the patient's point of view operational tol...
journal_title:Transplantation
pub_type: 历史文章,杂志文章
doi:10.1097/01.tp.0000117776.14277.03
更新日期:2004-03-27 00:00:00
abstract:BACKGROUND:Guillain-Barre Syndrome (GBS) is believed to be caused by autoimmune mechanisms that are predominantly T-cell mediated. We report GBS in organ transplant patients and bone marrow transplant patients, both of whom have iatrogenically suppressed T-cell function. METHODS:We reviewed the Duke University Medical...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104270-00026
更新日期:2001-04-27 00:00:00
abstract:BACKGROUND:Avoiding donor-specific antibody (DSA) is difficult for sensitized patients. Improved understanding of the risk of low level DSA is needed. METHODS:We retrospectively compared the outcomes of 954 patients transplanted with varied levels of baseline DSA detected by single antigen beads and B flow cytometric ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001619
更新日期:2017-10-01 00:00:00
abstract::It was the objective of these experiments to study the time-related changes in the responsiveness of the cellular elements of the immune system following contact with single non-H-2 or multiple H-2 histocompatibility antigens. The reactivity of spleen cells from mice that received injections of spleen cells bearing H-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198802000-00047
更新日期:1988-02-01 00:00:00
abstract::Data of 32,000 donors were utilized for a computer simulation to analyze the effect of selection parameters on the outcome of kidney transplants. If the HLA match grade is considered for organ allocation, the overall 1-year graft survival rate is up to 7% higher for first cadaver transplants and up to 12% higher for s...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND:Organ scarcity continues in solid organ transplantation, such that the availability of organs limits the number of people able to benefit from transplantation. Medical advancements in managing end-stage organ disease have led to an increasing demand for multiorgan transplant, wherein a patient with multiorga...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002788
更新日期:2020-02-01 00:00:00
abstract::Donor pancreas utilization rates for whole organ transplant have remained low and have decreased over time. To identify the reasons for nonuse of pancreas from donors who meet common baseline acceptance criteria, we examined Organ Procurement and Transplantation Network data from 2005 to 2007 and identified a subgroup...
journal_title:Transplantation
pub_type: 社论,评审
doi:10.1097/TP.0b013e3181e9fea4
更新日期:2010-09-15 00:00:00