Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology.

Abstract:

:Lipase production by Staphylococcus xylosus and Rhizopus oryzae was investigated using a culture medium based on a mixture of synthetic medium and supernatants generated from tuna by-products and Ulva rigida biomass. The proportion of the three medium components was optimized using the simplex-centroid mixture design method (SCMD). Results indicated that the experimental data were in good agreement with predicted values, indicating that SCMD was a reliable method for determining the optimum mixture proportion of the growth medium. Maximal lipase activities of 12.5 and 23.5 IU/mL were obtained with a 50:50 (v:v) mixture of synthetic medium and tuna by-product supernatant for Staphylococcus xylosus and Rhizopus oryzae, respectively. The predicted responses from these mixture proportions were also validated experimentally.

journal_name

Environ Technol

journal_title

Environmental technology

authors

Sellami M,Kedachi S,Frikha F,Miled N,Ben Rebah F

doi

10.1080/09593330.2013.765920

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

2259-66

issue

13-16

eissn

0959-3330

issn

1479-487X

journal_volume

34

pub_type

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