Abstract:
BACKGROUND:Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. In this study, the effects of triptolide on lipopolysaccharide (LPS)-induced ALI in rats were investigated. METHODS:Sixty-five male Sprague Dawley rats(approved by ethics committee of the First Affiliated Hospital of Soochow University) were randomly divided into five groups. The control group was injected with 2.5 mL saline/kg body weight via the tail vein and intraperitoneally with 1% dimethyl sulfoxide (DMSO) (n = 5). The L group was administered with 0.2% LPS dissolved in saline (5 mg/kg) to induce ALI via the tail vein (n = 15). The TP1, TP2, and TP3 groups were treated as rats in the L group and then intraperitoneally injected with 25, 50, and 100 μg triptolide/kg body weight, respectively (15 rats per group). Blood samples from the left heart artery were taken for blood gas analysis at 1 hour before injection and at 1, 3, 6, and 12 hours after saline and DMSO administration in the control group, LPS injection in the L group, and triptolide injection in the TP1, TP2, and TP3 groups. Lung wet-to-dry weight (W/D) ratio, diffuse alveolar damage (DAD) score, TNF-α levels, and mRNA and protein expression of toll-like receptor 4 (TLR4) were analyzed. RESULTS:Compared with the control group, the arterial partial pressure of oxygen (PaO2) declined (P <0.05), the W/D ratio and DAD score increased (P <0.05), and TNF-α levels in serum and bronchoalveolar lavage fluid (BALF) and mRNA and protein expression of TLR4 were significantly increased in the L group (P <0.05). Compared with the L group, PaO2 significantly increased in the TP2 and TP3 groups (P <0.05), while the W/D ratio and DAD score were significantly decreased in the TP2 and TP3 groups (P <0.05). TNF-α levels and mRNA and protein expression of TLR4 were significantly decreased in the TP2 and TP3 groups compared with the L group (P <0.05). CONCLUSIONS:Triptolide can ameliorate LPS-induced ALI by reducing the release of the inflammatory mediator TNF-α and inhibiting TLR4 expression.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Gao J,Zhan Y,Chen J,Wang L,Yang Jdoi
10.1186/2047-783X-18-58subject
Has Abstractpub_date
2013-12-17 00:00:00pages
58eissn
0949-2321issn
2047-783Xpii
2047-783X-18-58journal_volume
18pub_type
杂志文章,收录出版abstract::In the treatment of pulmonary diseases the inhalation of aerosols plays a key role - it is the preferred route of drug delivery in asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis. But, in contrast to oral and intravenous administration drug delivery to the lungs is controlled by additional par...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/2047-783x-14-s4-71
更新日期:2009-12-07 00:00:00
abstract:BACKGROUND:Hereditary non-polyposis colorectal cancer (HNPCC) is an autosomal dominant tumor syndrome predisposing to predominantly colorectal and endometrial cancer. In 90% of the cases, molecular analyses reveal microsatellite instabilities due to germline mutations in DNA mismatch repair (MMR) genes, mainly MLH1, MS...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-01-28 00:00:00
abstract:BACKGROUND:The expression of heparanase (HPSE) was associated with postoperative metastatic recurrence in patients with hepatocellular carcinoma (HCC). The six E-box binding sites in the core promoter of the HPSE gene suggested that transcription factors of E-box such as upstream stimulatory factor (USF) might regulate...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-014-0045-9
更新日期:2014-08-23 00:00:00
abstract::The routine diagnosis of tuberculosis and other mycobacterial diseases based on organism identification was completed during a period of one year in 730 patients, with serological IgG, IgM and IgA determination of antibodies against antigen 60 from M. bovis , strain BCG. The analysis was performed on 10 groups of pati...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-05-24 00:00:00
abstract:BACKGROUND:This study investigates the effects of human bone marrow-derived mesenchymal stem cell (hMSC) on migration and proliferation ability of hepatocellular carcinoma (HCC) with high- and low-metastatic potential. METHODS:The hMSC and transforming growth factor-β1 (TGFβ-1) gene infected hMSC were co-cultured with...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-015-0144-2
更新日期:2015-05-24 00:00:00
abstract::The purpose of the present study was to evaluate clinically the effect of an anaesthetic gel (lidocaine 20 mg/g as active agent) on pain sensitivity and early wound healing following nonsurgical periodontal therapy. A total of 40 patients with chronic periodontitis were enrolled in this randomized, split-mouth, double...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2007-05-29 00:00:00
abstract:BACKGROUND:More and more studies have confirmed that miRNAs play an important role in maintaining bone remodeling and bone metabolism. This study investigated the expression level of miR-206 in the serum of osteoporosis (OP) patients and explored the effect and mechanism of miR-206 on the occurrence and development of ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-021-00480-3
更新日期:2021-01-18 00:00:00
abstract::Polyadenosylation of nuclear enzymes is well known to regulate the cellular repair capacity after DNA damage. PARP mediates the transfer of poly-ADP-ribose moieties on itself and other nuclear proteins by the breakdown of NAD+. The present study investigated how modulation of PARP activity interferes with cell death i...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2003-10-22 00:00:00
abstract:BACKGROUND:Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate t...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0357-2
更新日期:2018-12-26 00:00:00
abstract:OBJECTIVE:This study aims to discuss the correlation between serum inflammatory cytokines and neurogenic pulmonary edema (NPE) in children with severe hand-foot-mouth disease (HFMD). METHODS:A total of 89 patients with severe HFMD were enrolled into this study. These patients were divided into two groups, according to...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0313-1
更新日期:2018-05-03 00:00:00
abstract:BACKGROUND:The more frequent use of endoscopic ultrasonography (EUS) leads to an increased number of diagnosed gastric submucosal tumors (G-SMT). Since until now rather little therapeutical success in respect of these tumors has been achieved, we evaluated our concept of watchful waiting and selective treatment of pati...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章
doi:
更新日期:2005-07-29 00:00:00
abstract:BACKGROUND:Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by T-cell-dependent B-cell activation and altered T-cell response. Co-stimulatory and co-inhibitory molecules regulate and exert T-cell differentiation, survival and cytokine production. CD134+ and PD-1+ T-cells in SLE patients are inc...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-019-0385-6
更新日期:2019-07-22 00:00:00
abstract::Endorectal ultrasonography (ERUS) and magnetic resonance imaging (MRI) allow exploring the morphology of the rectum in detail. Use of such data, especially assessment of the rectal wall, is an important tool for ascertaining the perianal fistula localization as well as stage of the cancer and planning it appropriate t...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/s40001-014-0078-0
更新日期:2015-01-14 00:00:00
abstract:BACKGROUND:The pathogenesis of central retinal artery occlusions (CRAO) varies, depending upon the underlying disease. An embolic origin of an occlusion often occurs. PATIENT AND METHODS:A 50-year-old man with an occlusion of the left internal carotid artery was examined because of a left central retinal artery occlus...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-04-19 00:00:00
abstract:BACKGROUND:Vascular injury is the main mechanism in pathophysiology of PR3-ANCA-associated vasculitis. Soluble serum thrombomodulin (sTM) is a membrane-bound receptor for thrombin expressed by vascular endothelial cells. - OBJECTIVE:The aim of study was to determine the blood levels of sTM in patients with PR3-ANCA-as...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-s4-268
更新日期:2009-12-07 00:00:00
abstract:BACKGROUND:Increasing incidence of multidrug-resistant Mycobacterium tuberculosis infections is hampering global tuberculosis control efforts. Kuwait is a low-tuberculosis-incidence country, and ~ 1% of M. tuberculosis strains are resistant to rifampicin and isoniazid (MDR-TB). This study detected mutations in seven ge...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-019-0397-2
更新日期:2019-12-05 00:00:00
abstract::The influence of heparin on Polymorphonuclear (PMN s) leukocytes was investigated using a new whole-blood cytofluorometric method (patent granted for the test with the number P 4334935.8-41) with Candida albicans and Staphylococcus aureus as test microorganisms. After comparing the effect of equal volumes of two widel...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-12-31 00:00:00
abstract::Here we illustrate a 74-year-old patient presenting with clinical signs of slowly progressive motor neuron disease. After electromyography demonstrated myopathic changes, a skeletal muscle biopsy was performed showing histological signs of granulomatous myositis. In extensive diagnostic workup underlying primary granu...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2008-07-28 00:00:00
abstract:BACKGROUND:The literature did not show clearly if a correlation between trunk and facial asymmetry exists. The aim of this study was to verify the association between trunk and facial asymmetries, and trunk and facial sagittal configuration in adolescents. METHODS:This is a cross-sectional screening study. It was carr...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-017-0280-y
更新日期:2017-09-29 00:00:00
abstract::Possible effects of electromagnetic fields on human brain activity were studied. In a single-blind, cross-over-designed and placebo-controlled study 36 volunteers were exposed firstly to an electromagnetic field originating form a MediLine "MEGA-WAVE 150/1" therapy instrument and secondly to a field originating from a...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-10-16 00:00:00
abstract::We investigated the hypothesis that religious commitment could help counter general affective distress, accompanying depressive symptoms, in older age. A total of 34 older adults, all catholic believers, completed self-reported questionnaires on the presence of depressive symptoms, religiosity, health, worry, and the ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-16-9-401
更新日期:2011-09-12 00:00:00
abstract:OBJECTIVE:Most industrialized countries experienced a change in dietary habits within the last 10 years. Growing obesity may result in an increased incidence of metabolic diseases as well as in a higher caries frequency. METHODS:In an interdisciplinary study, 842 elementary school children (414 girls and 428 boys, age...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2004-08-31 00:00:00
abstract:BACKGROUND:To compare the effect of totally thoracoscopic with conventional, open repair of atrial septal defect. METHODS:Forty atrial septal defect cases were divided into two groups by surgical approach: totally thoracoscopic approach (group A, n = 20) and conventional open approach (group B, n = 20). In group A, su...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章
doi:10.1186/2047-783X-19-13
更新日期:2014-03-11 00:00:00
abstract:BACKGROUND:Serotonin (5-HT) and its receptors are present in central, the brain stem, and peripheral, the carotid body, tissues controlling the ventilatory responses to hypoxia. The exact action of serotonin and its nature are, however, unsettled. We hypothesized that the discrepant results on the ventilatory action of...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-s2-128
更新日期:2010-11-04 00:00:00
abstract::We present an 18 year old woman suffering from cavernous transformation of the portal vein, hypersplenism and bleeding esophageal varices. Color flow Doppler sonography showed gallbladder wall varices. A review of the literature, revealed 76 cases of gallbladder varices. Mostly, these were associated with portal vein ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-09-20 00:00:00
abstract::The introduction of integrase inhibitors as a new drug class for treatment of HIV have added a very potent and extremely well tolerated new treatment option to the HIV-armentarium. Particularly in patients with previously developed drug resistance, the availability of new active drugs offers great hope. Moreover, the ...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/2047-783x-14-s3-1
更新日期:2009-11-24 00:00:00
abstract:BACKGROUND:Increasing rates of prosthetic joint infection (PJI) have presented challenges for general practitioners, orthopedic surgeons and the health care system in the recent years. The diagnosis of PJI is complex; multiple diagnostic tools are used in the attempt to correctly diagnose PJI. Evidence-based algorithms...
journal_title:European journal of medical research
pub_type: 杂志文章,meta分析
doi:10.1186/s40001-017-0245-1
更新日期:2017-03-09 00:00:00
abstract:OBJECTIVE:Evaluating the effects of prophylactic administration of IgM-enriched immunoglobulins (IVIG) on immunological- and clinical parameters in cardiac surgical patients. PATIENTS AND METHODS:41 patients were randomized to receive either an IgM-enriched immunoglobulin (Pentaglobin(R)) preparation (1,300 ml immunog...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-12-17 00:00:00
abstract:BACKGROUND:The immunological and clinical benefits of structured treatment interruptions (STIs) during primary HIV-1 infection remain largely unclear. PATIENTS AND METHODS:Eight patients identified during primary HIV-1 infection were immediately treated with HAART and underwent subsequent STIs after reaching complete ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-07-31 00:00:00
abstract:BACKGROUND:Patients with congenital absence of the portal vein (CAPV) often suffer from additional medical complications such as hepatic tumors and cardiac malformations. CASE PRESENTATION:Congenital absence of the portal vein (CAPV) is a rare malformation. We present a case of a 16-year-old Chinese girl with CAPV wit...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/s40001-014-0063-7
更新日期:2014-11-26 00:00:00