NMR visibility studies of N-delta proton of proximal histidine in deoxyhemoglobin in lysed and intact red cells.

Abstract:

:Recent in vivo 1H MRS studies of muscle oxygen levels by means of myoglobin (Mb) oxygenation have necessitated an investigation of the possible contamination from deoxy-hemoglobin (Hb). To determine the contribution of Hb in these NMR measurements, we studied the NMR visibility of histidyl N-delta proton of Hb in lysed and intact RBC (red blood cell) suspensions. We found that the visibility of deoxy-Hb was significantly lower in intact RBCs (ca. 16%) than the solution form at an equivalent concentration of beta heme-iron, even though the visibility of deoxy-Hb from lysed RBC and deoxy-Mb was almost identical. Our study also shows that visibility increases with RBC swelling. Based on the difference in concentration and compartmentation, we conclude that less than 17% contribution from Hb is expected in Mb measurements in vivo. The possible mechanisms which cause the low visibility of Hb in RBCs are discussed.

journal_name

Magn Reson Med

authors

Wang DJ,Nioka S,Wang Z,Leigh JS,Chance B

doi

10.1002/mrm.1910300616

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

759-63

issue

6

eissn

0740-3194

issn

1522-2594

journal_volume

30

pub_type

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