Radiation-induced adaptive response for protection against micronucleus formation and neoplastic transformation in C3H 10T1/2 mouse embryo cells.

Abstract:

:We have monitored the end points of cellular survival, micronucleus formation and neoplastic transformation frequency to assess adaptation to ionizing radiation in the C3H 10T1/2 mouse embryo cell system. Plateau-phase cells were pre-exposed to an adapting dose of 0.1 to 1.5 Gy low-dose-rate gamma radiation 3.5 h prior to an acute challenge dose of 4 Gy. No adapting dose improved clonogenic survival detectably, whether the cells were plated immediately after the acute exposure or held in plateau phase for 3.5 h before plating. However, all chronic adapting doses resulted in both a reduction in micronucleus frequency in binucleate cells and about a twofold reduction in neoplastic transformation frequency per viable cell when cells were subsequently exposed to the 4-Gy challenge dose. Our data suggest that a low-dose-rate pre-exposure to ionizing radiation induces an adaptive response in C3H 10T1/2 cells, and that this response enhances DNA double-strand break repair when cells are subsequently exposed to a second radiation dose. This enhanced repair appears to be error-free since these adapted cells are also less susceptible to radiation-induced neoplastic transformation.

journal_name

Radiat Res

journal_title

Radiation research

authors

Azzam EI,Raaphorst GP,Mitchel RE

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

S28-31

issue

1 Suppl

eissn

0033-7587

issn

1938-5404

journal_volume

138

pub_type

杂志文章
  • Risk of chronic myeloid and acute leukemia mortality after exposure to ionizing radiation among workers at four U.S. nuclear weapons facilities and a nuclear naval shipyard.

    abstract::A nested case-control study was conducted among workers at five U.S. nuclear facilities to evaluate leukemia mortality risk (excluding chronic lymphocytic) from ionizing radiation using worksite doses and adjusting for potential confounding. Conditional logistic regression was used to estimate the relative risk (RR) o...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/rr0724.1

    authors: Schubauer-Berigan MK,Daniels RD,Fleming DA,Markey AM,Couch JR,Ahrenholz SH,Burphy JS,Anderson JL,Tseng CY

    更新日期:2007-02-01 00:00:00

  • Peripheral lymphocyte response to PHA and T cell population among atomic bomb survivors.

    abstract::The percentage of T lymphocytes of atomic bomb survivors showed no change as a function of age or exposure dose. The percentage of T cells was slightly lower in malignant-tumor patients than in the control group, but was significantly higher in the group with chromosomal aberrations than in the control group. The perc...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Akiyama M,Yamakido M,Kobuke K,Dock DS,Hamilton HB,Awa AA,Kato H

    更新日期:1983-03-01 00:00:00

  • Characterization of X-ray-induced immunostaining of proliferating cell nuclear antigen in human diploid fibroblasts.

    abstract::The repair of X-ray-induced DNA damage related to the proliferating cell nuclear antigen (PCNA) was characterized in human diploid fibroblasts by an indirect immunofluorescence method. PCNA staining induced by X rays was lost after DNase I treatment but not after RNase treatment. The staining was not induced when ATP ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Miura M,Sasaki T,Takasaki Y

    更新日期:1996-01-01 00:00:00

  • Mechanisms of direct radiation damage in DNA, based on a study of the yields of base damage, deoxyribose damage, and trapped radicals in d(GCACGCGTGC)(2).

    abstract::Dose-response curves were measured for the formation of direct-type DNA products in X-irradiated d(GCACGCGTGC)(2)prepared as dry films and as crystalline powders. Damage to deoxyribose (dRib) was assessed by HPLC measurements of strand break products containing 3' or 5' terminal phosphate and free base release. Base d...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR1058.1

    authors: Swarts SG,Gilbert DC,Sharma KK,Razskazovskiy Y,Purkayastha S,Naumenko KA,Bernhard WA

    更新日期:2007-09-01 00:00:00

  • Cellular and molecular mechanisms underlying oxygen-dependent radiosensitivity.

    abstract::Molecular oxygen has long been recognized as a powerful radiosensitizer that enhances the cell-killing efficiency of ionizing radiation. Radiosensitization by oxygen occurs at very low concentrations with the half-maximum radiosensitization at approximately 3 mmHg. However, robust hypoxia-induced signal transduction c...

    journal_title:Radiation research

    pub_type: 杂志文章,评审

    doi:10.1667/RR13959.1

    authors: Liu C,Lin Q,Yun Z

    更新日期:2015-05-01 00:00:00

  • Evidence that unrejoined DNA double-strand breaks are not predominantly responsible for chromosomal radiosensitivity of AT fibroblasts.

    abstract::To examine more fully the nature of chromosomal radiosensitivity in ataxia telangiectasia (AT) cells, we employed 24-color combinatorial painting to visualize 137Cs gamma-ray-induced chromosome-type aberrations in cells of two AT and one normal primary human fibroblast strains irradiated in log-phase growth. As a meas...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/rr3255

    authors: Loucas BD,Cornforth MN

    更新日期:2004-11-01 00:00:00

  • Probabilities of radiation-induced inter- and intrachromosomal exchanges and their dependence on the DNA content of the chromosome.

    abstract::A biophysical model has been developed that is based on the assumptions that an interphase chromosome occupies a spherical territory and that chromosome exchanges are formed by the misrejoining of two DNA double-strand breaks induced within a defined interaction distance. The model is used to explain the relative freq...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/0033-7587(2001)156[0603:poriia]2.0.co;2

    authors: Wu H

    更新日期:2001-11-01 00:00:00

  • Cell killing, chromosomal aberrations, and division delay as thermal sensitivity is modified during the cell cycle.

    abstract::Synchronous Chinese hamster ovary cells were treated in G1 or S phase with cycloheximide or procaine hydrochloride before and during heating at 43 degrees C. Cycloheximide and procaine apparently act by different mechanisms, with cycloheximide inhibiting protein synthesis and procaine hydrochloride supposedly affectin...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Dewey WC,Li XL,Wong RS

    更新日期:1990-06-01 00:00:00

  • Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles.

    abstract::We demonstrate by western analysis that the expression levels of TP53 (formerly known as p53), CDKN1A (formerly known as p21Waf1), CDC2 (formerly known as p34cdc2), CCNB1 (cyclin B1) and RAD51 are significantly modulated in confluent, density-inhibited human diploid cell populations exposed to doses where only a small...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Azzam EI,de Toledo SM,Gooding T,Little JB

    更新日期:1998-11-01 00:00:00

  • Microdosimetry at middle age: some old experimental problems and new aspirations.

    abstract::The increasingly wider use of microdosimetry in fields other than pure radiobiological research, especially in "practical" domains such as radiation protection and medical physics, has created a number of new practitioners who are not fully acquainted with many of the experimental pitfalls which beset the aspiring mic...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Kliauga P

    更新日期:1990-10-01 00:00:00

  • Effects of chronic gamma irradiation on reproduction in the earthworm Eisenia fetida (Oligochaeta).

    abstract::Eisenia fetida were exposed continuously to (60)Co gamma radiation during two generations (F(0) and F(1)). Adult F(0) reproduction capacity (i.e., number of cocoons produced, hatchability and number of F(1) hatchlings) in controls and at five dose rates (0.18, 1.7, 4, 11 and 43 mGy/h) was measured over a 13-week expos...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR1012.1

    authors: Hertel-Aas T,Oughton DH,Jaworska A,Bjerke H,Salbu B,Brunborg G

    更新日期:2007-11-01 00:00:00

  • Postirradiation glucan administration enhances the radioprotective effects of WR-2721.

    abstract::Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Patchen ML,MacVittie TJ,Jackson WE

    更新日期:1989-01-01 00:00:00

  • Modulation of tumor cell proliferation and apoptosis by polyamine depletion in cells of head and neck squamous cell carcinomas.

    abstract::These studies were carried out to examine the capacity of alpha-difluoromethylornithine (DFMO) to modulate cell proliferation and apoptosis in cells of squamous cell carcinomas (SCCs) of the head and neck. Exposure of cells to DFMO (5 mM for 48 h) depleted intracellular putrescine and spermidine levels (greater than 5...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Bock JM,Pickart MA,Pink JJ,Harari PM

    更新日期:1999-12-01 00:00:00

  • Impact of Single Dose Photons and Carbon Ions on Perfusion and Vascular Permeability: A Dynamic Contrast-Enhanced MRI Pilot Study in the Anaplastic Rat Prostate Tumor R3327-AT1.

    abstract::We collected initial quantitative information on the effects of high-dose carbon (12C) ions compared to photons on vascular damage in anaplastic rat prostate tumors, with the goal of elucidating differences in response to high-LET radiation, using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Syngene...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR15459.1

    authors: Bendinger AL,Seyler L,Saager M,Debus C,Peschke P,Komljenovic D,Debus J,Peter J,Floca RO,Karger CP,Glowa C

    更新日期:2020-01-01 00:00:00

  • Transport of aminothiol radioprotectors into mammalian cells: passive diffusion versus mediated uptake.

    abstract::Water:n-octanol partition coefficients (KD) were determined for a series of radioprotective thiols to ascertain whether these could be used to estimate reliably their rates of uptake into mammalian cells by passive diffusion. Values of KD determined for thiols in 0.1 M potassium phosphate, pH 7.4, at 22 degrees C were...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Newton GL,Aguilera JA,Kim T,Ward JF,Fahey RC

    更新日期:1996-08-01 00:00:00

  • Radiation therapy among atomic bomb survivors, Hiroshima and Nagasaki.

    abstract::As a follow-up to the two previous surveys of radiation therapy among the atomic bomb survivors, a large-scale survey was performed to document (1) the number of radiation therapy treatments received by the atomic bomb survivors and (2) the types of radiation treatments conducted in Hiroshima and Nagasaki. The previou...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Kato K,Antoku S,Russell WJ,Fujita S,Pinkston JA,Hayabuchi N,Hoshi M,Kodama K

    更新日期:1998-06-01 00:00:00

  • Serum Amyloid A as a Biomarker for Radiation Exposure.

    abstract::There is a need for minimally invasive biomarkers that can accurately and quickly quantify radiation exposure. Radiation-responsive proteins have applications in clinical medicine and for mass population screenings after a nuclear or radiological incident where the level of radiation exposure and exposure pattern comp...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR13927.1

    authors: Sproull M,Kramp T,Tandle A,Shankavaram U,Camphausen K

    更新日期:2015-07-01 00:00:00

  • Radiosensitivity of murine T-lymphocyte cytotoxicity.

    abstract::A biphasic curve was observed when surviving allogeneic lytic activity was plotted as a function of irradiation delivered before sensitization. Flow cytometry analysis demonstrated that the number of cells was reduced in response to increasing dose and that subset precursors Lyt 1+2+ were proportionally more radiosens...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Gerber M

    更新日期:1984-11-01 00:00:00

  • Lung cancer in French and Czech uranium miners: Radon-associated risk at low exposure rates and modifying effects of time since exposure and age at exposure.

    abstract::Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR0848.1

    authors: Tomasek L,Rogel A,Tirmarche M,Mitton N,Laurier D

    更新日期:2008-02-01 00:00:00

  • The effect of radiation quality on genomic DNA methylation profiles in irradiated human cell lines.

    abstract::It has been acknowledged for many years that radiation exposure induces delayed, non-targeted effects in the progeny of the irradiated cell. Evidence is beginning to demonstrate that among these delayed effects of radiation are epigenetic aberrations, including altered DNA methylation. To test the hypothesis that diff...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR2390.1

    authors: Goetz W,Morgan MN,Baulch JE

    更新日期:2011-05-01 00:00:00

  • Use of a Humanized Mouse Model System in the Validation of Human Radiation Biodosimetry Standards.

    abstract::After a planned or unplanned radiation exposure, determination of absorbed dose has great clinical importance, informing treatment and triage decisions in the exposed individuals. Biodosimetry approaches allow for determination of dose in the absence of physical measurement apparatus. The current state-of-the-art biod...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR15283.1

    authors: Pujol-Canadell M,Young E,Smilenov L

    更新日期:2019-05-01 00:00:00

  • Impact of therapeutic irradiation on healthy articular cartilage.

    abstract::Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage...

    journal_title:Radiation research

    pub_type: 杂志文章,评审

    doi:10.1667/RR13928.1

    authors: Saintigny Y,Cruet-Hennequart S,Hamdi DH,Chevalier F,Lefaix JL

    更新日期:2015-02-01 00:00:00

  • Stimulation of hematopoietic cell recovery by tetrachlorodecaoxide in sublethally irradiated mice.

    abstract::This study investigated whether tetrachlorodecaoxide (TCDO), a potent wound-healing agent, promotes recovery of hematopoietic tissue of mice depleted by sublethal X irradiation. Mice were exposed to 3 Gy total-body irradiation and were given 1 ml/kg TCDO intravenously daily from day 1 to 5 or day 4 to 8 after irradiat...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Mason KA,Murphy S,Milas L

    更新日期:1993-11-01 00:00:00

  • Low-dose binary behavior of bystander cell killing after microbeam irradiation of a single cell with focused c(k) x rays.

    abstract::Although conclusive evidence has been obtained for the presence of radiation-induced bystander effects, the mechanisms that trigger and regulate these processes are still largely unknown. The bystander effect may play a critical role in determining the biological effectiveness of low-dose exposures, but questions on h...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/rr3319

    authors: Schettino G,Folkard M,Michael BD,Prise KM

    更新日期:2005-03-01 00:00:00

  • The biological effectiveness of radon-progeny alpha particles. III. Quality factors.

    abstract::Domestic radon risk estimates are typically based either on data for uranium miners or on data derived from A-bomb survivors; comparison of domestic radon risk estimates derived from these two disparate sources represents an important test of their reliability. There is currently a significant discrepancy of about a f...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Brenner DJ,Miller RC,Huang Y,Hall EJ

    更新日期:1995-04-01 00:00:00

  • The effect of dimethyl sulfoxide on the induction of DNA double-strand breaks in V79-4 mammalian cells by alpha particles.

    abstract::The present study was undertaken to assess the protective effect of dimethyl sulfoxide (DMSO) against the induction and rejoining of DNA double-strand breaks (DSBs) and inactivation of V79-4 Chinese hamster cells by both high- and low-linear energy transfer (LET) radiations. The cells were exposed under aerobic condit...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: deLara CM,Jenner TJ,Townsend KM,Marsden SJ,O'Neill P

    更新日期:1995-10-01 00:00:00

  • Cerebrovascular Remodeling and Neuroinflammation is a Late Effect of Radiation-Induced Brain Injury in Non-Human Primates.

    abstract::Fractionated whole-brain irradiation (fWBI) is a mainstay of treatment for patients with intracranial neoplasia; however late-delayed radiation-induced normal tissue injury remains a major adverse consequence of treatment, with deleterious effects on quality of life for affected patients. We hypothesize that cerebrova...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR14616.1

    authors: Andrews RN,Metheny-Barlow LJ,Peiffer AM,Hanbury DB,Tooze JA,Bourland JD,Hampson RE,Deadwyler SA,Cline JM

    更新日期:2017-05-01 00:00:00

  • Postirradiation alterations of neuronal chromatin structure.

    abstract::Previous work from our laboratory suggested that neuronal chromatin structure may be altered immediately after exposure to ionizing radiation. In the present study, whole brains of 4-month-old male Fisher 344 rats were irradiated with a dose of 25 Gy. The kinetics of restoring the chromatin structure to its unirradiat...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Jaberaboansari A,Nelson GB,Roti Roti JL,Wheeler KT

    更新日期:1988-04-01 00:00:00

  • The Beta 2 Adrenergic Receptor Antagonist Timolol Improves Healing of Combined Burn and Radiation Wounds.

    abstract::In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR14884.1

    authors: Albrecht H,Yang HY,Kiuru M,Maksaereekul S,Durbin-Johnson B,Wong MS,Stevenson TR,Rocke DM,Isseroff RR

    更新日期:2018-04-01 00:00:00

  • Acute radiation-induced vomiting in area postrema-ablated cats.

    abstract::A dose-response relationship was established in normal unanesthetized cats for emetic incidence, latency to onset of vomiting, and duration of emetic activity over a period of 24 h after whole-body exposure to 60Co radiation at selected doses between 7.5 and 90 Gy. Each episode of vomiting, i.e., retching and expulsio...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Borison HL,McCarthy LE,Douple EB,Johnson J,Borison R

    更新日期:1987-03-01 00:00:00