Abstract:
:The higher ordered structure of the chicken alpha 2(1) procollagen gene was analyzed in chromatin isolated from expressing (lung) and nonexpressing (reticulocyte and erythrocyte) tissues. Digestion of DNA with methylation sensitive restriction endonucleases revealed that this gene was methylated in all tissues examined and that no differences existed in the promoter methylation patterns between expressing and nonexpressing tissues. DNAse 1 hypersensitive sites were located between 100-300 bp upstream from the transcription initiation site and within the first intron. These sites were also hypersensitive to the single-strand specific S1 nuclease, implying that this region of the gene in the chromatin is either in an unfolded single-stranded conformation or under severe conformational stress. These differences in the alpha 2(1) chromatin structure were confirmed by the finding that the promoter was more accessible to restriction endonuclease digestion in the expressing tissues than in the nonexpressing tissues. Digestion of chromatin with Pst I and Sma I revealed that some of these sites in the promoter were differentially protected by DNA-binding proteins in the two tissue types. These protected sites were located as far upstream as -1,600 and downstream within the first intron at +800.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Truter SL,Parker MIdoi
10.1002/jcb.240540204subject
Has Abstractpub_date
1994-02-01 00:00:00pages
154-60issue
2eissn
0730-2312issn
1097-4644journal_volume
54pub_type
杂志文章abstract::SCN- binds to the charged amino group of lysines, inducing local changes in the electrostatic free energy of histones. We exploited this property to selectively perturb the histone-DNA interactions involved in the stabilization of eu and heterochromatin. Differential scanning calorimetry (DSC) was used as leading tech...
journal_title:Journal of cellular biochemistry
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pub_type: 杂志文章
doi:10.1002/jcb.24688
更新日期:2014-03-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.25677
更新日期:2017-09-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24851
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journal_title:Journal of cellular biochemistry
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1002/jcb.240530308
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更新日期:2018-12-02 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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journal_title:Journal of cellular biochemistry
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更新日期:2008-05-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.26706
更新日期:2018-07-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22548
更新日期:2010-05-15 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240260207
更新日期:1984-01-01 00:00:00