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Molecular epidemiology of human papillomavirus infections in cervical samples from cuban women older than 30 years.

Abstract：

OBJECTIVE:This study aimed to provide information about the molecular epidemiology of human papillomavirus (HPV) in a group of Cuban women. MATERIALS AND METHODS:DNA from cervical samples was analyzed using a quantitative real-time polymerase chain reaction (PCR), which detects 6 of the clinically most relevant high-risk HPV types. Furthermore, end point PCR and sequencing were performed. Three hundred twenty-two women (211 with positive and 111 with negative cytologic results) aged between 30 and 69 years were enrolled. Risk factors associated with HPV infections and premalignant lesions were also investigated. RESULTS:HPV DNA was detected in 76.1% (245/322) of the studied population, and 34 different genotypes were found. There was an association between HPV infection and low educational level, history of oral contraceptives, menopausal stage, as well as cigarette and/or alcohol consumption. Besides, in a multivariate analysis, previous positive Pap test result and positive colposcopy finding were both predictor variables for HPV infections and for premalignant lesions. Human papillomavirus infection was found in 94.3% of women (199/211) with positive cytologic result and in 41.4% (46/111) of those with negative results, being more likely that the first group was infected with any HPV (odds ratio = 23.43; 95% CI = 11.70-46.92; p = .000). The most common genotypes were HPV types 16, 18, 31, 58, 33, and 45. All the cases with HPV positive findings had at least 1 high-risk HPV genotype. CONCLUSIONS:This is the first report of the molecular epidemiology of HPV in Cuban women, based on results from a DNA sequence and quantitative PCR. Most individuals were infected with high-risk HPV types. These findings support the inclusion of HPV vaccine in Cuba.

journal_name

J Low Genit Tract Dis

journal_title

Journal of lower genital tract disease

authors

Soto Y,Torres G,Kourí V,Limia CM,Goicolea A,Capó V,Pérez L,de la Torre AI,López LX,Govín A,Correa CB,Alemán Y,Alvarez AA,Manzano BR

doi

10.1097/LGT.0b013e3182a7bb89

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

210-7

issue

eissn

1089-2591

issn

1526-0976

journal_volume

pub_type

杂志文章

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Molecular epidemiology of human papillomavirus infections in cervical samples from cuban women older than 30 years.