Abstract:
:We describe a new monoclonal antibody No. 204 (mcAb 204) which recognized a family of four polypeptides, consisting of a 27kD, a 24/23kD double band and a 19kD protein present within PEDV infected cell lysates. These proteins were identified by immunoprecipitation as well as by staining of immunoblots. In infected Vero cell cultures, the synthesis of the 27kD protein was initiated between 6 and 8 hours post inoculation. The 24/23kD double band and the 19kD protein were only detectable later. At least the 27 and the 24/23kD proteins were apparently glycosylated and present in purified virions. Pulse-chase as well as solubilization experiments indicated that the faster migrating bands represented processed products of the 27kD glycoprotein. The nature of the processing is not known at present. We suggest that the 27kD protein family may represent the integral membrane protein M of PEDV. Since this protein is highly abundant in virions as well as in infected cells, and since mcAb 204 is able to react with its antigen under various conditions, this monoclonal antibody may be useful to further studies of the M-protein of PEDV. In addition, it may provide a useful tool for routine diagnosis.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Utiger A,Rosskopf M,Guscetti F,Ackermann Mdoi
10.1007/978-1-4615-2996-5_31subject
Has Abstractpub_date
1993-01-01 00:00:00pages
197-202eissn
0065-2598issn
2214-8019journal_volume
342pub_type
杂志文章abstract::Herbert Hultgren, a cardiologist who kept a careful diary all his career, arrived in Peru at the beginning of 1959 to study the occurrence of pulmonary hypertension in children at high altitude with patent ductus arteriosus. There, he was told about a strange condition, high altitude pulmonary edema (HAPE), and found ...
journal_title:Advances in experimental medicine and biology
pub_type: 传,历史文章,杂志文章
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pub_type: 杂志文章,评审
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doi:10.1007/978-1-4684-8730-5_18
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pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-3404-4_22
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1007/978-981-13-1426-1_8
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doi:10.1007/978-1-4615-0713-0_57
更新日期:2002-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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abstract::The Utah electrode array (UEA) and its many derivatives have become a gold standard for high-channel count bi-directional neural interfaces, in particular in human subject applications. The chapter provides a brief overview of leading electrode concepts and the context in which the UEA has to be understood. It goes on...
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doi:10.1007/978-981-13-2050-7_1
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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更新日期:2019-01-01 00:00:00
abstract::In the cardiovascular and hematopoietic systems the Transient Receptor Potential Canonical 3 (TRPC3) channel has a well-recognized role in a number of signaling mechanisms that impact the function of diverse cells and tissues in physiology and disease. The latter includes, but is not limited to, molecular and cellular...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-26974-0_9
更新日期:2016-01-01 00:00:00
abstract:UNLABELLED:Premature infants require large amounts of protein and energy to achieve normal growth. Feeding with human milk alone is therefore only regarded acceptable if the protein and energy content is adequate. METHODS:476 milk samples from 101 mothers delivering before the 32nd gestational week (mean gestational a...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-1371-1_51
更新日期:2001-01-01 00:00:00