Abstract:
:Several reports addressed the issue of how the alternative splicing of exon 7 and 8 in the APP pre-mRNA is regulated in different tissues. Of special interest here was the potential involvement of exon 7 containing APP splice isoforms, since this exon codes for a serine protease inhibitor and is therefore of putative relevance for amyloidogenic catabolism of the precursor protein. The recent identification of a third alternative splice site in close proximity to the beta A4-amyloid portion in the APP gene which may also increase APP amyloidogenicity, allowed us to investigate its regulation in cells of the central nervous system. With our assay, we were able to resolve six different APP isoforms of the eight potential isoforms which can be generated from the three alternatively spliced exons 7, 8, and 15. We demonstrate here that, in addition to rat brain microglia cells, astrocyte-enriched cultures also skip the novel alternative 3'-splice site in front of exon 15, generating L-APP mRNA. Neurons are the only cells in the central nervous system which seem to use the 3'-splice site of intron 14 nearly 100%. Interestingly, this very 3'-splice site is the only one present in the APP gene that completely matches the consensus sequence for the branchpoint sequence proposed for introns. We would therefore suggest that neurons lack a specific splicing factor which inhibits the use of the rather strong 3'-splice site in front of exon 15. It remains to be shown whether this is also the case for neurons in Alzheimer's disease.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Sandbrink R,Banati R,Masters CL,Beyreuther K,König Gdoi
10.1111/j.1749-6632.1993.tb23049.xsubject
Has Abstractpub_date
1993-09-24 00:00:00pages
183-9eissn
0077-8923issn
1749-6632journal_volume
695pub_type
杂志文章,评审abstract::Intracerebral administration of the excitotoxin ibotenate to new-born mice induced white-matter lesions mimicking the periventricular leukomalacia occurring in human premature babies. In this model, co-injection of vasoactive intestinal peptide (VIP) prevented white-matter lesions. VIP did not prevent the initial appe...
journal_title:Annals of the New York Academy of Sciences
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abstract::We are used to thinking of mathematics as a symbolic activity. Here I argue that it may also be seen as a performative one. Making an analogy with music, I draw attention to the ways in which mathematics may not only be written down but also played out. ...
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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abstract::The data presented above suggest that one possible clinical use of TGF-beta would be to protect the bone marrow from the effects of myelosuppressive chemotherapeutic drugs by preventing entry or removing primitive stem cells from the cell cycle. It may also have the additional benefit of reducing the drug-induced neut...
journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
pub_type: 评论,杂志文章,评审
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更新日期:2006-12-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
abstract::Ischemia and reperfusion injure the heart, as manifested by myocardial infarction, postischemic ventricular functional dysfunctions, arrhythmias, and cardiomyocyte apoptosis. Hearts can be adapted to ischemic-reperfusion injury by subjecting them to non-lethal cyclic episodes of short-term ischemia and reperfusion. Th...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1999-06-30 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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更新日期:2005-06-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2004-10-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1991.tb24459.x
更新日期:1991-12-31 00:00:00
abstract::Frozen-hydrated microtubules have been studied by cryoelectron microscopy. We have described some structural features of microtubules, their relationship with the computed or X ray diffraction patterns, and the influence of the contrast transfer function. ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1986.tb34489.x
更新日期:1986-01-01 00:00:00
abstract::Clostridium difficile infection, also known as C. difficile-associated diarrhea (CDAD), is the most common cause of nosocomial diarrhea, typically initiated by the use of broad-spectrum antibiotics that disrupt gut flora, thereby allowing C. difficile to proliferate. It is an increasing cause of morbidity and mortalit...
journal_title:Annals of the New York Academy of Sciences
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更新日期:2013-07-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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更新日期:2008-12-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1992.tb25964.x
更新日期:1992-06-28 00:00:00
abstract::Social support promotes resilience to adverse childhood events, but little is known about the role of natural mentors-caring, nonparental adults-in the lives of childhood abuse survivors. The current study draws from a large, longitudinal, nationally representative sample (Add Health) to examine the prevalence and cha...
journal_title:Annals of the New York Academy of Sciences
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更新日期:2021-01-01 00:00:00
abstract::A high index of suspicion is essential in arriving at the correct diagnosis of Lambert-Eaton myasthenic syndrome (LEMS). LEMS should be considered in the differential in any patient who has proximal weakness, reduced or absent muscle stretch reflexes, and dry mouth. Weakness predominates in hip and shoulder muscles, b...
journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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更新日期:2005-06-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2014-04-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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更新日期:2010-07-01 00:00:00