Abstract：
:We have developed a method that enables the rates of phagocytosis and killing of bacteria by neutrophils to be measured in a single assay. Neutrophils were incubated with bacteria, and at specific intervals were separated from uningested bacteria by low speed centrifugation. Rates of phagocytosis and killing were calculated from the decrease in number of extracellular bacteria and change in the number of intracellular bacteria. Both phagocytosis and killing were shown to follow first-order kinetics, and rate constants were calculated without having to separate the assay into two phases. In contrast to two-step methods, our method measures killing from the moment the neutrophils start ingesting the bacteria, and also eliminates the need to halt neutrophil activity temporarily and restart the assay after the extracellular bacteria have been removed. We obtained reproducible results for the phagocytosis and killing of Staphylococcus aureus (t1/2 = 9 min and 6 min respectively) and Escherichia coli (t1/2 = 10 min and 2 min respectively). We also were able to detect a 56% impairment in the rate of killing of S. aureus by neutrophils from an individual with a low level of myeloperoxidase.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Hampton MB,Vissers MC,Winterbourn CCdoi
10.1002/jlb.55.2.147subject
Has Abstractpub_date
1994-02-01 00:00:00pages
147-52issue
2eissn
0741-5400issn
1938-3673journal_volume
55pub_type
杂志文章abstract：:Antagonism of ROS signaling can inhibit cell apoptosis and autophagy, thus favoring the maintenance and expansion of hematopoietic stem cells. Alpha lipoic acid (ALA), a small antioxidant molecule, affects cell apoptosis by lowering the ROS level. In this study, we show that ALA promoted production of human pluripoten...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/JLB.1A0520-179R
更新日期：2020-12-01 00:00:00
abstract：:Previously, we showed that NO induces thymocyte apoptosis via a caspase-1-dependent mechanism [(1) ]. In the present study, we investigated the role of heme oxygenase, catalase, bax, and p53 in this process. The NO donor, S-nitroso-N-acetyl penicillamine (SNAP), induced DNA fragmentation in thymocytes in a time- and c...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:The aim of the study was to elucidate the effect of lipopolysaccharide (LPS) administration in vivo (Escherichia coli endotoxin, 1 mg/kg body weight) on the expression and cellular activity of glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), the rate-limiting enzyme of the hexose monophosphate shunt in hepatic ...
journal_title：Journal of leukocyte biology
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更新日期：1994-10-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1109714
更新日期：2010-06-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.57.6.875
更新日期：1995-06-01 00:00:00
abstract：:To determine if the release of IL-1 alpha and IL-1 beta by cultured PBMC could be independently modulated by different exogenous stimuli, we examined the effect of LPS, IFN gamma, latex beads, and indomethacin on the release of IL-1 alpha and IL-1 beta. PBMC culture supernatants were fractionated by Sephacryl-S-200 co...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.46.5.417
更新日期：1989-11-01 00:00:00
abstract：:Interleukin-8 (IL-8), a neutrophil-activating cytokine, also activates certain T cell functions such as chemotaxis. We additionally find (n = 6) that recombinant (rIL-8; 1-100 ng/ml), when added to 24 h culture of human CD4+ T cells, suppressed the spontaneous production of IL-4 (50-85%). Steady state production of Il...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.59.3.407
更新日期：1996-03-01 00:00:00
abstract：:Cytokines and chemokines play an essential role in recruiting leukocytes from the circulation to the peripheral sites of inflammation by modulating cellular interactions with endothelial cell ligands and extracellular matrix (ECM). Herein, we examined regulation of T cell adhesion to ECM ligands by two major proinflam...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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journal_title：Journal of leukocyte biology
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doi：10.1002/JLB.3MA0220-355R
更新日期：2020-07-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.66.1.59
更新日期：1999-07-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
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更新日期：2012-03-01 00:00:00
abstract：:Leukocyte adhesion is a key factor in the pathogenesis of organ injury following a variety of stimuli. In this study we have addressed the role of leukocyte adhesion in hypertensives as a risk factor for organ injury. In the spontaneously hypertensive rat (SHR), the number of circulating leukocytes and their level of ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.42.2.95
更新日期：1987-08-01 00:00:00
abstract：:Resting microglia comprise up to 13% of the cells in human central nervous system (CNS) white matter. Their large number and dendritic morphology make them ideally suited to survey the CNS for noxious stimuli. Upon activation microglia gradually lose dendritic processes and transform into typical phagocytic macrophage...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.56.6.732
更新日期：1994-12-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.47.1.49
更新日期：1990-01-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.52.4.390
更新日期：1992-10-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1004564
更新日期：2005-08-01 00:00:00
abstract：:Lymphocyte development is initiated from hematopoietic stem cells and can be divided into multiple phenotypically distinct stages. Transcription factors play important roles in programming the developmental process of lymphocytes. Recent studies have identified key roles of several orphan nuclear receptors in T lympho...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：
更新日期：2002-09-01 00:00:00
abstract：:IL-27, an IL-12 family member, was initially described as a proinflammatory cytokine. Nevertheless, it also poses anti-inflammatory activity, being involved in suppressing development of TH-17 cells as well as in the induction of inhibitory Tr1 cells. Recent data obtained in mice suggest that it can down-modulate the ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1209788
更新日期：2011-06-01 00:00:00
abstract：:Introduction of alloantigens into the AC induces a form of immune tolerance known as ACAID, which induces antigen-specific CD8+ Tregs, contributing to ocular immune privilege by down-regulating immune responses. Recent evidence suggests IFN-γ is needed for the suppressive function of CD8+ ACAID Tregs. This study teste...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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journal_title：Journal of leukocyte biology
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更新日期：2011-06-01 00:00:00
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更新日期：2016-11-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0805468
更新日期：2006-06-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1002/JLB.3RI0519-161R
更新日期：2019-11-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.0303089
更新日期：2003-12-01 00:00:00
abstract：:It is well established that the cytokine IL-12 and the transcription factor STAT4, an essential part of the IL-12 signaling pathway, are critical components of the Th1 differentiation process in T cells. In response to pathogenic stimuli, this process causes T cells to proliferate rapidly and secrete high amounts of t...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1A0814-375RR
更新日期：2015-10-01 00:00:00
abstract：:Lymphocyte function-associated antigen (LFA-1) is a member of the beta2 family of integrins that is selectively expressed on leukocytes. Herein, we show that Ca(2)(+) mobilizing agents A23187, thapsigargin, and ionomycin induce an increase in adhesion to the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1) and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:In this study we present data on a novel cell surface antigen recognized by monoclonal antibody (mAb) VPM30, originally thought to recognize only bovine and ovine sIg+ B cells from peripheral blood. Here we show that the antigen, molecular mass 28 kDa, is not only found in B cell follicles in frozen sections, but when...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.63.5.567
更新日期：1998-05-01 00:00:00
abstract：:The interaction between CD40 on dendritic cells (DC) and its ligand CD154 has been recognized to be an important feature in the maturation of DC. Here, we were interested in the role of CD44 a surface receptor shown to mediate cell-cell adhesion and binding to Hyaluronic acid (HA). Western blot analysis of human DC st...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:DC maturation and functions are influenced by microbial and environmental stimuli, which could contribute to immune dysfunction. Here, we have investigated the role of enterobacteria (Escherichia coli CBL2 and Shigella CBD8) isolated from CD patients, bifidobacteria (Bifidobacterium longum CECT 7347 and Bifidobacteriu...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1111581
更新日期：2012-11-01 00:00:00
