Abstract：
:This study compared the responses of canine and human platelets to various aggregating agonists in the presence or absence of extracellular ATP and ATP analogues. Canine and human platelets were approximately equally reactive with ADP or collagen while the canine platelets were about 10 fold more sensitive to thrombin. Canine platelets were insensitive to the thromboxane mimetic U46619 but were synergistically aggregated by a mixture of ADP and U46619. Human platelets were very sensitive to U46619. Aggregations of human platelets with all of the above agonists were inhibited by extracellular ATP; beta, gamma methylene ATP (beta gamma ATP) and benzoyl ATP (BzATP) with a rank order suggestive of an interaction with P2x-like purinoceptors which support our previous findings. The comparable aggregations of canine platelets were likewise inhibited by ATP and its analogues but with a rank order suggestive of an interaction with P2y-like purinoceptors. ATP inhibited U46619- and ADP-induced aggregation of human platelets and ADP-induced aggregation of canine platelets, presumably, in part, due to competition for the ADP P2T receptor. However, when U46619 was added to either ATP or ATP analogue-inhibited ADP-treated canine platelets, the inhibition was nullified. Furthermore, we demonstrated, for the first time, that the canine thromboxane receptor becomes reactive to U46619 alone after incubation at room temperature for 3.5-5 hrs while human platelets become inactive under similar conditions. The implication of these studies is that there are significant differences in the canine and human platelet thromboxane and purine receptors. The future characterization of these differences and the mechanism by which they function should further our understanding of the impact of extracellular ATP on hemostasis and thrombosis.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Soslau G,Arabe L,Parker J,Pelleg Adoi
10.1016/0049-3848(93)90214-9subject
Has Abstractpub_date
1993-10-15 00:00:00pages
127-37issue
2eissn
0049-3848issn
1879-2472pii
0049-3848(93)90214-9journal_volume
72pub_type
杂志文章abstract：:Anticoagulant properties of three sulfated compounds prepared from xylans isolated from corn cobs, larchwood and oatspelts were compared with heparin and sodium pentosan polysulfate (SP-54) by studying their effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) using poo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90148-4
更新日期：1987-08-01 00:00:00
abstract：INTRODUCTION:Inflammation and coagulation play important roles in the pathogenesis of sepsis. Anticoagulants with anti-inflammatory action draw attention as therapeutic agent in sepsis. OBJECTIVE:Whether cilostazol (6-[4-(1-cyclohexyl-1H-tetrazol-5-yl) butoxy]-3,4-dihydro-2-(1H)-quinolinone), anticoagulant, protects m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.06.017
更新日期：2015-08-01 00:00:00
abstract：:The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90008-c
更新日期：1993-01-01 00:00:00
abstract：:Both venous thromboembolism and intracranial metastases are common complications in the setting of primary brain tumors and metastatic malignancies. Anticoagulation is indicated in the presence of cancer-associated thrombosis in order to limit the risk of pulmonary embolism; however, there is reluctance to initiate an...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(16)30100-1
更新日期：2016-04-01 00:00:00
abstract：:The thrombolytic efficacy of recombinant tissue-type plasminogen activator (tPA) in the presence and absence of a thromboxane synthase inhibitor was studied in a model of femoral artery thrombosis in the anesthetized rabbit. The thrombus was formed by injection of thrombin and whole blood into an isolated segment of t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90022-9
更新日期：1988-12-01 00:00:00
abstract：BACKGROUND:The "HERDOO2 rule" is a prospectively validated clinical decision rule used to identify low-risk women who can safely discontinue anticoagulants after completing 5-12 months of anticoagulant treatment for unprovoked venous thromboembolism. The VIDAS®d-Dimer (DD) assay, a component of the rule, was used in th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.07.020
更新日期：2018-09-01 00:00:00
abstract：:The Calibrated Automated Thrombogram (CAT), a plate-based assay that measures thrombin generation and inhibition in plasma samples, is modified to measure the procoagulant activity of phospholipid associated with plasma microparticles (MP). The assay uses a tissue factor trigger without addition of 4 μM exogenous phos...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.10.018
更新日期：2011-01-01 00:00:00
abstract：:Human saphenous veins were analyzed for presence of plasminogen activators (t-PA) in the endothelium of the vessel walls by immunohistochemical techniques. We used a polyclonal rabbit antihuman t-PA antibody, a monoclonal mouse-antihuman t-PA and a goat antibody to low molecular weight urokinase. To demonstrate presen...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90240-9
更新日期：1986-02-01 00:00:00
abstract：:The study of the interaction between activated protein C (APC) and non-plasmatic inhibitors allowed us to demonstrate that aprotinin is a potent competitive inhibitor of APC with a Ki of 1.35 mumol/L. It was possible to adsorb immunopurified protein C (PC) activated by venom activator to insolubilized aprotinin and to...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90268-h
更新日期：1990-07-01 00:00:00
abstract：INTRODUCTION:Electronic consultations (eConsults) are asynchronous, consultative electronic-based communications that are aimed to improve efficient and timely access to specialist advice. Our study aim was to evaluate the use and impact of the Canadian Champlain BASE™ (Building Access to Specialists through eConsultat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.11.002
更新日期：2017-12-01 00:00:00
abstract：INTRODUCTION:Antiphospholipid syndrome (APS) is a pro-thrombotic autoimmune disease that affects different vascular beds, with potential risk for recurrence. Systemic lupus erythematosus (SLE), specific autoantibodies profile and atherogenic disorders have been described as risk factors for the occurrence of first thro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.10.029
更新日期：2015-12-01 00:00:00
abstract：:Liquid permeation studies of fibrin gels, which were formed in the presence of thrombin (Fibrin II) and in the presence of Batroxobin (Fibrin I), showed that those gels have distinctly different flow properties. For both gels the permeability coefficient (Ks) and average pore size varied depending on the conditions fo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90214-6
更新日期：1982-01-01 00:00:00
abstract：BACKGROUND:Integrin αIIbβ3 mediates platelet adhesion, aggregation and fibrin clot retraction. These processes require activation of αIIbβ3 and post-ligation signaling. Disulfide bond exchanges are involved in αIIbβ3 and αvβ3 activation. METHODS:In order to investigate the role of integrin activation and disulfide bon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.02.001
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND AND AIM:Recent studies have documented that elevation of C-reactive protein (CRP) levels after percutaneous coronary intervention (PCI) have been predictive of adverse outcome. This study was performed to test the hypothesis that preprocedural use of naproxen sodium is associated with a reduction in the exte...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2006.11.003
更新日期：2007-01-01 00:00:00
abstract：:Platelet aggregation in platelet-rich plasma (PRP) is more sensitive to agonists and more resistant to antagonists than that in whole blood. In this study, we show that the presence of neutrophils in whole blood accounts at least in part for diminished platelet aggregation in whole blood. The platelet-inhibitory effec...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90216-y
更新日期：1990-12-01 00:00:00
abstract：:Interaction with circulating platelets is considered an important virulent mechanism for Staphylococcus aureus (S. aureus) bacteria to induce endocarditis, a severe infectious disease with high incidence of systemic thrombosis. It therefore represents an important target for pharmacological intervention. In this study...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.11.016
更新日期：2005-01-01 00:00:00
abstract：:S-antigen (also named arrestin or 48K protein) is a protein abundant in photoreceptor cells of vertebrates and invertebrates. The presently known function of this protein in retina is to arrest the enzymatic cascade of phototransduction in retinal rods, through its binding to photoactivated and phosphorylated rhodopsi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Platelets occupy a central role at the interface between thrombosis and inflammation. At sites of vascular damage, adherent platelets physically and functionally interact with circulating leukocytes. Activated platelets release soluble factors into circulation that may have local and systemic effects on blood and vasc...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2010.10.010
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND:Deep vein thrombosis (DVT) is a common complication of acute stroke, but the new incidence in the era of improved specialist input in stroke care is yet unknown. The models for VTE diagnosis is well established, but prediction models to target at-risk patients for pharmacological prophylaxis is lacking and r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.002
更新日期：2016-06-01 00:00:00
abstract：:Cancer is frequently associated with activation of blood coagulation, which in turn has been suggested to promote tumor growth and metastasis. Indeed, low molecular weight heparin treatment significantly prolongs the survival of a wide variety of patients with cancer. Based on this notion that anticoagulant treatment ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(12)70022-1
更新日期：2012-04-01 00:00:00
abstract：:A new type of assay for heparins has been devised, in which the test material competes with 125I-labelled heparin for binding to protamine-Sepharose. The assay is very sensitive and will measure heparin concentrations down to 10 ng ml-1. It responds to both the degree of sulphation and the molecular weight of acidic p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90056-1
更新日期：1982-08-15 00:00:00
abstract：BACKGROUND AND OBJECTIVE:According to US Food and Drugs Administration (FDA), 2 hour recombinant tissue plasminogen activator (rt-PA) 100mg infusion is recommended for eligible patients with acute pulmonary embolism (PE). However,there exists evidence implying that a lower dosage of rt-PA can be equally effective but p...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2013.12.026
更新日期：2014-03-01 00:00:00
abstract：INTRODUCTION:Different Klebsiella pneumoniae strains carry different virulence factors and antibiotic resistance and may cause thrombocytopenia. This study aimed to investigate the effects of different infections caused by K. pneumoniae on platelets. METHODS:Two hypermucoviscous K. pneumoniae strains and two classic s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.09.053
更新日期：2018-11-01 00:00:00
abstract：:It has become customary to regard the various glycerophospholipids as quite similar, and the acyl groups are considered to have little influence on the behaviour of the lipids in membranes or metabolism. Nevertheless, a number of recent observations by the authors and others indicate a high degree of metabolic compart...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/0049-3848(92)90006-v
更新日期：1992-08-01 00:00:00
abstract：:Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low den...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90182-c
更新日期：1990-11-01 00:00:00
abstract：INTRODUCTION:High concentrations of N-terminal-pro-brain natriuretic peptide (NT-proBNP) are found in patients with right ventricular overload. Right ventricular overload may be the result of large perfusion defects in patients with pulmonary embolism (PE). NT-proBNP levels are associated with poorer outcome in patient...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.02.014
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:The level of platelet reactivity (PR) inhibition obtained after P2Y12-ADP receptor antagonist loading dose (LD) is associated with the ischemic and bleeding risk following percutaneous coronary intervention (PCI) in acute coronary syndromes (ACS). OBJECTIVE:We aimed to evaluate the level of PR inhibition ac...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2013.04.030
更新日期：2013-07-01 00:00:00
abstract：:The role of free radical generation and its scavenging enzymes in circulating mice polymorphonuclear leukocytes (PMNLs) has been studied following pulmonary thromboembolism. Levels of malonaldehyde (MDA), 02- radical generation, activity of superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), myelo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90183-b
更新日期：1992-05-01 00:00:00
abstract：:Human platelets synthesize nitric oxide (NO) through an endothelial-type NO synthase (ecNOS) activated also by substances enhancing 3',5'-cyclic adenosine monophosphate (cAMP) concentrations, such as catecholamines, beta-adrenoceptor agonists and adenosine. To verify whether cAMP directly activates ecNOS through the c...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.06.036
更新日期：2004-01-01 00:00:00
abstract：:In rabbits, a clear microscopic image of individual, fluorescent blood platelets flowing in a mesenteric microvessel could be obtained over the whole cross-sectional area of the vessel, following an intravenous injection of a solution containing 5-15 mg of the fluorochrome Acridine Red. Most of the circulating platele...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90106-2
更新日期：1982-12-15 00:00:00
