Inhibition of Na,K-ATPase by cadmium: different mechanisms in different species.

Abstract:

:The mechanism of action of Cd on Na,K-ATPase was investigated in two "classical" model systems, the shark rectal gland and rabbit kidney outer medulla. In lyophilized plasma membranes from dogfish rectal gland Cd inhibited Na,K-ATPase activity after 30 min of preincubation with an I50 of 1.3 x 10(-5) M. K-Dependent p-nitrophenylphosphatase (pNPPase) activity was inhibited 50% by Cd at 9.4 x 10(-6) M. Neither Na nor K altered the interaction of the enzyme with Cd. Decreasing the ATP concentration, however, lowered the apparent sensitivity of Na,K-ATPase to Cd. The inhibitory effect was also significantly reduced when the Mg concentration present during the preincubation was increased from 0.5 to 6.0 mM. The apparent Cd sensitivity of the K-dependent pNPPase is lower at 10 mM Mg than at 1 mM Mg. In initial rate experiments 4 x 10(-5) M Cd increased the apparent Km of the enzyme for Mg significantly from 0.88 +/- 0.29 mM to 1.73 +/- 0.3 mM whereas the Vmax (167 +/- 32 mumol/min x mg protein compared to 140 +/- 16 mumol/min x mg protein) remained essentially unchanged. In lyophilized rabbit kidney outer medulla, Cd was found to inhibit Na,K-ATPase activity with an I50 of 1.9 x 10(-5) M. K-Dependent pNPPase was inhibited 50% under identical conditions by Cd at a nominal concentration of 2.1 x 10(-4) M. Increasing K concentrations protected the enzyme from the inhibitory action of Cd as indicated by a 10-fold decrease in sensitivity of pNPPase when the K concentration was increased from 1 to 20 mM. K, 20 mM, delayed also the onset of inhibition by about 15 min at 37 degrees C. These studies suggest that the mode of action of Cd on Na,K-ATPase varies in different species. In rectal gland Cd competes with a Mg site (or sites) critically involved in ATP and pNPP hydrolysis, whereas in rabbit renal medulla Cd interacts with a potassium-binding site. Differences in the protein sequence, protein conformation, and/or in the kind of protein membrane-lipid interaction might contribute to this diversity observed in the inhibitory mechanisms.

journal_name

Toxicol Appl Pharmacol

authors

Kinne-Saffran E,Hülseweh M,Pfaff C,Kinne RK

doi

10.1006/taap.1993.1124

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

22-9

issue

1

eissn

0041-008X

issn

1096-0333

pii

S0041008X83711245

journal_volume

121

pub_type

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