Abstract：
:Despite high vaccination coverage, pertussis remains an important respiratory infectious disease and the least-controlled vaccine-preventable infectious disease in children. Natural infection with Bordetella pertussis is known to induce strong and long-lasting immunity that wanes later than vaccine-mediated immunity. Therefore, a live attenuated B. pertussis vaccine, named BPZE1, has been developed and has recently completed a phase I clinical trial in adult human volunteers. In this study, we investigated the contribution of adenylate cyclase (CyaA) in BPZE1-mediated protection against pertussis. A CyaA-deficient BPZE1 mutant was thus constructed. Absence of CyaA did not compromise the adherence properties of the bacteria onto mammalian cells. However, the CyaA-deficient mutant displayed a slight impairment in the ability to survive within macrophages compared to the parental BPZE1 strain. In vivo, whereas the protective efficacy of the CyaA-deficient mutant was comparable to the parental strain at a vaccine dose of 5 × 10(5) colony forming units (CFU), it was significantly impaired at a vaccine dose of 5 × 10(3) CFU. This impairment correlated with impaired lung colonization ability, and impaired IFN-γ production in the animal immunized with the CyaA-deficient BPZE1 mutant while the pertussis-specific antibody profile and Th17 response were comparable to those observed in BPZE1-immunized mice. Our findings thus support a role of CyaA in BPZE1-mediated protection through induction of cellular mediated immunity.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Lim A,Ng JK,Locht C,Alonso Sdoi
10.1016/j.micinf.2013.10.002subject
Has Abstractpub_date
2014-01-01 00:00:00pages
51-60issue
1eissn
1286-4579issn
1769-714Xpii
S1286-4579(13)00194-9journal_volume
16pub_type
杂志文章abstract：:Since it was first described as having the ability to inhibit macrophage activation, transforming growth factor-beta (TGF-beta) has been analyzed for its role in regulating immune responses to a variety of pathogens, including viruses, bacteria, yeast, and protozoa. Most of the studies have involved organisms that inf...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(99)00252-x
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abstract：:In vitro cell culture models used to study how Salmonella initiates disease at the intestinal epithelium would benefit from the recognition that organs and tissues function in a three-dimensional (3-D) environment and that this spatial context is necessary for development of cultures that more realistically resemble i...
journal_title：Microbes and infection
pub_type： 杂志文章
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abstract：:Oropouche virus is the aetiological agent of Oropouche fever, a zoonotic disease mainly transmitted by midges of the species Culicoides paraensis. Although the virus was discovered in 1955, more attention has been given recently to both the virus and the disease due to outbreaks of Oropouche fever in different areas o...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
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abstract：:Trypanosoma cruzi the cause of Chagas disease persists in tissues of infected experimental animals and humans. Here we demonstrate the persistence of the parasite in adipose tissue from of three of 10 elderly seropositive patients with chronic chagasic heart disease. Nine control patients had no parasites in the fat. ...
journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2011.06.002
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2010.10.020
更新日期：2011-03-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2002-09-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2011.08.011
更新日期：2012-01-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2013.03.006
更新日期：2013-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2004.12.012
更新日期：2005-03-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2019.03.001
更新日期：2019-01-01 00:00:00
abstract：:The Gram-negative bacterium Burkholderia cepacia has recently emerged as an important opportunistic pathogen in humans. This review focuses on the cellular aspects of B. cepacia infection and the dynamics of the B. cepacia-host cell interaction, including recent advances in our understanding of the ability of B. cepac...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(01)01389-2
更新日期：2001-04-01 00:00:00
abstract：:Population genetic structure and subdivision are key factors affecting the evolution of organisms. In this study, we analysed and compared the population genetic structure of the malaria parasite Plasmodium falciparum and its mosquito vector Anopheles gambiae over space and time in the Nianza Province, near Victoria L...
journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2007.12.021
更新日期：2008-03-01 00:00:00
abstract：:In this report, we examined the genetic diversity of HIV-1 strains circulating in the city of Beira, the second largest metropolitan area in Mozambique. A total of 131 blood samples, collected between August and October 2003 from antiretroviral-naïve individuals, were characterized with a combined approach consisting ...
journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2006.05.005
更新日期：2006-08-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2016.07.004
更新日期：2016-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2009.08.012
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journal_title：Microbes and infection
pub_type： 信件
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更新日期：2020-01-01 00:00:00
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journal_title：Microbes and infection
pub_type：
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2003.05.001
更新日期：2003-12-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2004.08.001
更新日期：2004-11-01 00:00:00
abstract：:Amoebiasis is responsible for 50000-100000 deaths annually. Invasive amoebic disease begins with the attachment of Entamoeba histolytica trophozoites to colonic mucin, a process mediated by the amoebic Gal/GalNAc lectin. The non-pathogenic counterpart, E. dispar, is morphologically identical but genetically distinct. ...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2000-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2000-12-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2015-08-01 00:00:00
abstract：:Interleukin (IL)-15 is a recently described cytokine that resembles IL-2 in its biological activities, stimulating natural killer cells, T cells and B cells to proliferate, secrete cytokines, and exhibit increased cytotoxicities or produce antibody. IL-15 also exerts unique functions such as stimulation of phagocytes,...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(00)00329-4
更新日期：2000-04-01 00:00:00
abstract：:Gliotoxin is a mycotoxin having a considerable number of immuno-suppressive actions and is produced by several moulds such as Aspergillus fumigatus. In this study, we investigated its toxic effects on human neutrophils at concentrations corresponding to those found in the blood of patients with invasive aspergillosis....
journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2006.10.009
更新日期：2007-01-01 00:00:00
