Short communication: HIV type 1 tropism determination in a novel dried blood spot membrane and the use of a mixture of outer nested polymerase chain reaction primers.

Abstract:

:Genotropism was determined in 608 Brazilian samples collected in dried blood spots using Polyethersulfone collection cards. Patients were infected by subtype B (88.8%), F (5.6%), C (3.3%), A (1.8%), and G (0.5%). All patients were exposed to three classes of antiretrovirals, and 59.8% of the samples harbored R5 viruses, 35% non-R5-tropic viruses, and 5.1% harbored mixtures of R5 and non-R5-tropic viruses, with non-R5 more prevalent among clade B-infected patients as compared to non-B (42.8% versus 19.1%; p<0.0003). A strategy using a mixture of outer nested polymerase chain reaction (PCR) primers reduced the number of negative PCR results from 39% to 19%.

authors

Lavigne S,Santos C,Arif MS,Reis A,Samer S,Dos Santos CV,Diaz RS

doi

10.1089/AID.2012.0261

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

147-50

issue

2

eissn

0889-2229

issn

1931-8405

journal_volume

30

pub_type

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