Abstract:
:Advanced glycation end products (AGEs) are stable end products of the Maillard reaction. Effects of food extracts are often initially analysed in cellular test systems and it is not clear how different cell culture conditions might influence the results. Therefore, we compared the effects of two models for AGE-rich food, bread crust and coffee extract (CE) on WI-38 human lung fibroblasts under different cell culture conditions (sub-confluent versus confluent cells, with and without serum). WI-38 cells responded to coffee and bread crust extract (BCE) with a rapid phosphorylation of PKB (AKT), p42/44 MAPK (ERK 1/2) and p38 MAPK, strongly depending on culture conditions. BCE resulted in increased cell numbers, whereas CE appeared to be cytotoxic. When cell numbers under all culture conditions and treatments were correlated with kinase phosphorylation, the relation between phospho-p38 MAPK and phospho-AKT represented a good, cell culture condition-independent predictor of cell survival.
journal_name
Int J Food Sci Nutrjournal_title
International journal of food sciences and nutritionauthors
Nass N,Weissenberg K,Somoza V,Ruhs S,Silber RE,Simm Adoi
10.3109/09637486.2013.839631subject
Has Abstractpub_date
2014-03-01 00:00:00pages
219-25issue
2eissn
0963-7486issn
1465-3478journal_volume
65pub_type
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