Inhibition of RNA synthesis by benzene metabolites and their covalent binding to DNA in rabbit bone marrow mitochondria in vitro.

Abstract:

:Mitoplasts from rabbit bone marrow cells in vitro incubated with benzene metabolites showed a concentration-dependent inhibition of mitochondrial RNA synthesis. The 50% molar inhibitory concentration for each metabolite was determined to be p-benzoquinone, 2 X 10(-6); phenol, 2.5 X 10(-5); hydroquinone, 5 X 10(-5); catechol, 2 X 10(-3); benzene, 1.6 X 10(-2). Activated metabolites of radiolabeled benzene covalently bound to mitochondrial DNA in vitro. Labeled deoxynucleosides, enzymatically produced from DNA isolated from mitoplasts prelabeled in DNA with [3H]dGTP and exposed to [14C]benzene in vitro, were chromatographed on a Sephadex LH-20 column to obtain deoxynucleoside-adducts. The elution profiles indicated that mtDNA contained seven deoxyguanosine-adducts. A similar experiment in which the mtDNA was prelabeled with [3H]dATP indicated that two deoxyadenosine-adducts of mtDNA were formed from benzene in vitro. Tentative identification of several deoxyguanosine-adducts indicates that p-benzoquinone, hydroquinone, phenol, and 1,2,4-benzenetriol produced from benzene form adducts with guanine.

journal_name

Am J Ind Med

authors

Kalf GF,Snyder R,Rushmore TH

doi

10.1002/ajim.4700070512

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

485-92

issue

5-6

eissn

0271-3586

issn

1097-0274

journal_volume

7

pub_type

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