Abstract:
:In this study, radiolabeled recombinant rat interleukin-1 beta (r125I-IL-1 beta) was used to localize and characterize IL-1 beta binding in rat hypothalamus and pituitary gland by quantitative autoradiography. The ability of this ligand to bind to type I IL-1 receptor was first tested on murine lymphoma cells (EL-4). In the rat-tissue sections, high densities of specific r125I-IL-1 beta binding sites were localized in the anterior as well as the posterior pituitary and in the choroid plexus. A fine labeling was observed in meninges and third ventricle walls while no binding was detected in the hypothalamic nuclei. Saturation experiments, in the anterior and posterior pituitary, revealed one specific binding site with an affinity constant (Kd) of 0.5 nM. Competition experiments were achieved using either rat IL-1 beta (rIL-1 beta) or human IL-1s (hIL-1 alpha, hIL-1 beta and IL-1 receptor antagonist: hIL-1a). Affinity constants (Ki) were drastically different according to the ligand used, while Ki values were found similar in anterior and posterior pituitary. Competition with rIL-1 beta revealed one binding affinity (Ki of 0.1 nM range). In contrast, competition with hIL-1 beta revealed two binding affinities: a high (Ki: 0.1 pM range) and a low one (Ki: 1 nM range). Competition with hIL-1ra was obtained for high concentrations only (Ki: 10-100 nM range), whereas human IL-1 alpha (hIL-1 alpha) was unable to compete at 1-100 nM.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Marquette C,Van Dam AM,Ban E,Lanièce P,Crumeyrolle-Arias M,Fillion G,Berkenbosch F,Haour Fdoi
10.1159/000127026subject
Has Abstractpub_date
1995-10-01 00:00:00pages
362-9issue
4eissn
0028-3835issn
1423-0194journal_volume
62pub_type
杂志文章abstract::Distribution and properties of receptors for gonadotropin-releasing hormone (GnRH) were analyzed in the brain of adult male rats. Binding of the iodinated GnRH agonist Des-Gly10-(D-Ala6)-GnRH ethylamide was studied in hippocampus and anterior pituitary using three convergent approaches: quantitative autoradiography on...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125053
更新日期:1988-11-01 00:00:00
abstract::The timing of the episodic secretion of luteinizing hormone (LH) in ovariectomized ewes was investigated with respect to an ultradian rest-activity cycle. Individual sheep were enclosed in a chamber where their physical activity was continuously monitored, and blood samples for LH analysis were collected at 5-min inte...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123187
更新日期:1981-06-01 00:00:00
abstract::A consistent group of gonadotropin-releasing hormone (GnRH) cell bodies occurs in the ventral hypothalamic tract at the infundibular lip (IL), just below the arcuate nucleus (ARC), at the site of the so-called GnRH 'pulse generator'. Immunocytochemical studies were performed to examine contacts between these GnRH neur...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124914
更新日期:1988-03-01 00:00:00
abstract::Hyponatremia due to elevated arginine vasopressin (AVP) secretion increases mortality in liver failure patients. The mechanisms causing dysregulation of AVP secretion are unknown. Our hypothesis is that inappropriate AVP release associated with liver failure is due to increased brain-derived neurotrophic factor (BDNF)...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000503723
更新日期:2020-01-01 00:00:00
abstract::Although previously considered rare, recent epidemiological studies have revealed that the incidence (3.6/100,000) and prevalence (35/100,000) of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) has increased over the past few decades. Despite the progress in the understanding of GEP-NET molecular biology, ther...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000362339
更新日期:2014-01-01 00:00:00
abstract::There is a great deal of conflicting data regarding the issue of whether androgens influence opiate receptors in the whole male rat brain. Although Hahn and Fishman initially reported that long-term castration produced a large increase in the density of opiate-binding sites, relative to controls, no other independent ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124816
更新日期:1987-08-01 00:00:00
abstract::A reproductive facet of ghrelin, a stomach-derived orexigenic peptide involved in energy homeostasis, has been recently suggested, and predominantly inhibitory effects of ghrelin upon luteinizing hormone (LH) secretion have been demonstrated in rat models. Yet, the modulatory actions of ghrelin on the gonadotropic axi...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000092753
更新日期:2005-01-01 00:00:00
abstract::Grafts of normal mouse preoptic area (POA) tissue into the third ventricle of gonadotrophin-releasing hormone (GnRH)-deficient hypogonadal (hpg) mice resulted in an elevation of pituitary GnRH receptors, an increased synthesis of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by the pituitary gland, a...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124762
更新日期:1987-05-01 00:00:00
abstract::The parenchymal cells of the pars intermedia (PI) and corticotrophs of the pars distalis (PD) synthesize pro-opiomelanocortin (POMC), which, through posttranslational processing, gives rise to a group of structurally related peptides, including MSHs, ACTH, CLIP, LPHs and endorphins. We investigated the control of rele...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124203
更新日期:1985-11-01 00:00:00
abstract::Growth hormone-releasing hormone (GHRH) stimulates GH secretion in man and the hormonal response is specific. The attenuation of GH response to bolus GHRH after prior exposure of GHRH of up to 24 h was not demonstrated in normal or GH-deficient subjects after more prolonged exposure. This suggests that the partial los...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000125793
更新日期:1991-01-01 00:00:00
abstract::Serotonin, a biologically active amine, is related to carcinoid syndrome in functioning neuroendocrine tumors (NETs). Telotristat ethyl is a novel inhibitor of the tryptophan hydroxylase (TPH), a key enzyme in the production of serotonin. While its use in patients with carcinoid syndrome and uncontrolled diarrhea unde...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000502200
更新日期:2020-01-01 00:00:00
abstract::In man and other mammals, starvation is accompanied by a severe suppression of luteinizing hormone-releasing hormone (LHRH) and luteinizing-hormone (LH) secretion, which is caused by unknown alterations in hypothalamic functions. Prostaglandin E2 (PGE2), endorphins and testosterone (T) are know to be strongly involved...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124034
更新日期:1984-12-01 00:00:00
abstract::In pituitary cells, c-fos transcription induced by releasing hormones and growth factors results from enhanced initiation of transcription, and sustained elongation of transcripts beyond the first intron. We studied the regulatory role of the first intron of the mouse c-fos gene for the control of its transcription in...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054606
更新日期:2000-12-01 00:00:00
abstract::Observations of long standing have suggested that the 'stress' of chair restraint inhibits the GnRH pulse generator in normal female monkeys while this phenomenon is rarely observed in ovariectomized animals. The role of the ovary in the response of the GnRH pulse generator to the stress of insulin hypoglycemia was in...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126291
更新日期:1992-11-01 00:00:00
abstract::Delta-sleep-inducing peptide (DSIP) colocalizes within gonadotropin-releasing-hormone (GnRH)-containing neurons in adult hypothalamus and could play a role in the regulation of hypothalamic-pituitary axis in adults. To support the possibility that DSIP also participates in fetal neuroendocrine events and to demonstrat...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126098
更新日期:1992-01-01 00:00:00
abstract::The hypothalamic neurons containing substance P (SP) were examined immunohistochemically in rats with a normal estrous cycle or persistent estrus (PE), in pregnancy and in lactating state. In PE animals, the neurons increased in number 1 week after ovariectomy, but then decreased and disappeared. During pregnancy, the...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124764
更新日期:1987-05-01 00:00:00
abstract:INTRODUCTION:Small-intestinal neuroendocrine tumors (SI-NET) are situated preferentially within the ileum. The aim was to describe a potential difference in location between unifocal and multiple ileal-NET. METHODS:Between December 2010 and December 2019, all consecutive patients who underwent resection in our Europea...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000511849
更新日期:2020-09-30 00:00:00
abstract::Leptin exerts important effects on the regulation of food intake and energy expenditure by acting in the brain. Leptin action is mediated by the interaction with a receptor that is alternatively spliced, resulting in at least five different isoforms. The long form (OB-Rb) has a long intracellular domain that is essent...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054536
更新日期:2000-03-01 00:00:00
abstract::High affinity binding of 3H-flunitrazepam (FNZP) to crude membrane preparations of bovine pineal membranes was examined by a rapid filtration procedure through Whatman GFB paper. At 0 degrees C binding reached equilibrium in about 20 min. Scatchard analysis of data at equilibrium revealed a single population of bindin...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123533
更新日期:1983-08-01 00:00:00
abstract::We have suggested that gamma-aminobutyric acid (GABA) in the hypothalamus plays a tonic inhibitory role in the control of the luteinizing hormone (LH) release in intact male rats. To assess whether feedback from the testis alters the inhibitory GABAergic tone in the medial preoptic area (MPO) of male rats, an in vivo ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000066625
更新日期:2002-11-01 00:00:00
abstract::The possible implication of the amygdaloid central nucleus (ACE) of the rat in the control of ACTH secretion in response to immobilization stress was assessed. The ACTH secretion, in response to stress and/or bilateral lesions of the ACE, was correlated with the serotoninergic activity in specific hypothalamic and amy...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124652
更新日期:1986-01-01 00:00:00
abstract::Because of some indication that FSH secretion is less dependent than LH secretion on GnRH in vivo, we performed experiments to examine the effects of a GnRH antagonist (antag) on LH and FSH secretion. We first showed that pituitary cells superfused with GnRH showed a similar pattern of suppressed secretion of both LH ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124081
更新日期:1985-03-01 00:00:00
abstract::We have investigated the effects of exposure to short-term constant light (LL) on the spontaneous, proestrous luteinizing hormone (LH) surge in the female rat. Exposure to LL during the 3 days preceding proestrus delayed and reduced the magnitude of the LH surge which was measured in blood samples taken from conscious...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123225
更新日期:1981-09-01 00:00:00
abstract::This study was designed to investigate the effects of penfluridol, a potent neuroleptic calmodulin inhibitor, on basal and secretagogue-stimulated secretion of thyroid-stimulating hormone (TSH), growth hormone (GH), and luteinizing hormone (LH). The drug had no effect on basal TSH or LH release, but decreased GH relea...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123548
更新日期:1983-09-01 00:00:00
abstract::The injection of purified alpha-bungarotoxin (alpha-BTX), an antagonist of the muscle nicotinic cholinergic receptors, into the third ventricle (3rd V) of ovariectomized rats decreased the frequency of pulses of luteinizing hormone (LH) but had no significant effect on the amplitude or nadir of LH secretion. Approxima...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125153
更新日期:1989-05-01 00:00:00
abstract::In vivo and in vitro methods have been used to compare the effects of opioid receptor blockade on the functional activity of the hypothalamo-pituitary-gonadal axis in adult (200 g) and sexually immature (50 g) male rats. In the adult, a single injection of the mu-receptor antagonist, naloxone (500 micrograms/100 g bod...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125777
更新日期:1991-06-01 00:00:00
abstract::Corticotropin-releasing factor (CRF) has been characterized on the basis of its intrinsic activity to release corticotropin from cultured rat anterior pituitary cells. Injected in intact rats, CRF increases adrenocorticotropic hormone (ACTH) release. Endogenous CRF-like immunoreactivity was detected in the cytoplasm a...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125186
更新日期:1989-06-01 00:00:00
abstract::The modulatory actions of ovarian steroids on the norepinephrine (NE) induced alterations in luteinizing hormone (LH) secretion were examined in male and female rats. Long-term (4 weeks) castrated male and female rats bearing chronic third-ventricle cannulae were implanted with intra-atrial catheters. Animals were ble...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124581
更新日期:1986-01-01 00:00:00
abstract::Diurnal variations, ranging over a twofold difference in concentrations of dopamine, norepinephrine and epinephrine, occur in the rat posterior pituitary gland during a 24-hour period. Highest catecholamine concentrations were seen during daylight hours and low levels reached a nadir 5 h after the onset of darkness. D...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123292
更新日期:1982-02-01 00:00:00
abstract::The present study was undertaken to evaluate the 24-hour periodicity in serum prolactin levels subsequent to transecting the ascending serotonergic system or ablating the suprachiasmatic nuclei (SCN) of adult (185 g) female rats. The ascending serotonergic system was transected at the level of the rostral mesencephalo...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123055
更新日期:1980-08-01 00:00:00