Abstract:
:Concentrations of triglycerides are increased and concentrations of high-density lipoprotein (HDL) cholesterol are low in women with hyperandrogenism. These alterations could be related to excessive androgen or estrogen, to hyperinsulinism, or to a combination of these abnormalities. We examined their independent influences on lipids in 21 women with hyperandrogenism, subgrouped according to apparent source of androgen excess. Results for lipid, androgen, and insulin did not differ among subgroups, so these data were pooled. Free plus albumin-bound testosterone (uT) was correlated with triglycerides (r = 0.69, P less than 0.01) and HDL cholesterol (r = -0.56, P less than 0.01). Both triglycerides (r = 0.66, P less than 0.01) and HDL cholesterol (r = -0.48, P less than 0.05) were also correlated with insulin measured during fasting. Partial correlation revealed that, after adjusting for insulin, lipids were associated with uT. This suggests that androgen excess is independently related to lipid excess. Insulin also was correlated with lipids when adjusted for uT. Free plus albumin-bound estradiol was not associated with any of the lipids. We conclude that altered lipids in women with hyperandrogenism result from the independent effects of androgen and insulin.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Wild RA,Applebaum-Bowden D,Demers LM,Bartholomew M,Landis JR,Hazzard WR,Santen RJsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
283-9issue
2eissn
0009-9147issn
1530-8561journal_volume
36pub_type
杂志文章abstract:BACKGROUND:New genetic forms of hereditary hemochromatosis (HH) or hereditary hyperferritinemia (HF) have been identified over the last few years, and abnormalities of various genes may interact in a single patient. This study aimed to develop a rapid automated method for sequencing the main genes involved. METHODS:We...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2007.090605
更新日期:2007-12-01 00:00:00
abstract::Serum specimens from patients on primidone therapy were assayed by two currently available procedures: a commercially available enzyme immunoassay (EMIT) and a published gas-chromatographic procedure. Both procedures were done with commercially available materials and equipment. Results by the two procedures agreed we...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract:BACKGROUND:An accelerated stability model based on the Arrhenius Equation can be used to estimate stability of diagnostic reagents. Here we review 3 examples in which the model does not accurately predict the stability of diagnostic reagents. METHODS:We prepared several pilot lots of quality controls materials contain...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1093/clinchem/hvaa282
更新日期:2020-12-04 00:00:00
abstract::We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly sim...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:Previous studies have shown a single linear relationship between mean plasma glucose (MPG) and hemoglobin A(1c) (HbA(1c)). We examined the relationship in different treatment groups of patients with type 1 diabetes participating in the Diabetes Control and Complications Trial (DCCT). METHODS:Seven-point glu...
journal_title:Clinical chemistry
pub_type: 杂志文章,随机对照试验
doi:10.1373/clinchem.2006.079756
更新日期:2007-05-01 00:00:00
abstract::We studied 25 borderline-hyperparathyroidism patients whose total serum calcium concentration was within normal limits (reference range: 2.25--2.75 mmol/liter) but whose concentrations of serum ionized calcium were above normal (reference range: 1.03--1.23 mmol/liter). Their hyperparathyroidism was histopathologically...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract::Single-spin density-gradient ultracentrifugation (DUC) has proven to be a reproducible method for detection of low-density-lipoprotein (LDL) heterogeneity. Recently another method has been described for this: gradient gel electrophoresis (GGE) of serum, a method that might be more suitable for screening. To gain insig...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that h...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Throughout the 1970s, controversy centered both on immunoassay "sensitivity" per se and on the relative sensitivities of labeled antibody (Ab) and labeled analyte methods. Our theoretical studies revealed that RIA sensitivities could be surpassed only by the use of very high-specificity nonisotopic labels in "noncompe...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1991-11-01 00:00:00
abstract::I determined the in vitro biological half-lives or decay constants for creatine kinase isoenzymes at various temperatures. Values at 37 degrees C are consistent with values reported by others in vivo, which suggests that in vivo irreversible inactivation is primarily thermal. Reversible inactivation appears to be an o...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::We describe a procedure for determining amitriptyline and imipramine and their active metabolites nortriptyline and desipramine, respectively, at therapeutic concentrations in human plasma by use of liquid chromatography. The drugs are extracted at pH 10.5 into hexane/isoamyl alcohol, which is evaporated and the resid...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND:ADB-PINACA and its 5-fluoropentyl analog 5F-ADB-PINACA are among the most potent synthetic cannabinoids tested to date, with several severe intoxication cases. ADB-PINACA and 5F-ADB-PINACA have a different legal status, depending on the country. Synthetic cannabinoid metabolites predominate in urine, making ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2016.267575
更新日期:2017-05-01 00:00:00
abstract::This new one-step chemiluminescent immunoassay of free thyroxin (FT4) involving a thyroxin-immunoglobulin conjugate labeled with acridinium ester (Magic Lite System; Ciba Corning Diagnostics Corp., Medfield, MA) is rapid (one 1-h incubation), requires two calibrators per run, and takes 10 s per sample for the quantifi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::In this method for serum and urinary urea determination, the same reagent is used without predilution of urine samples. The method is based on the pH increase resulting from the ammonia released by urease hydrolysis of urea. o-Cresolphthalein complexone is used to monitor the pH change colorimetrically. Urea concentra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Haptoglobin is a hemoglobin-binding protein expressed by a genetic polymorphism as three major phenotypes: 1-1, 2-1, and 2-2. Most attention has been paid to determining haptoglobin phenotype as a genetic fingerprint used in forensic medicine. More recently, several functional differences between haptoglobin phenotype...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-10-01 00:00:00
abstract::Even in the absence of detectable ethanol or visible lipemia, a large plasma osmolal gap is the usual finding in cases of diabetic ketoacidosis. This gap decreases to an insignificant value within 20 h of treatment. Detailed biochemical analysis of six cases showed that, on average, the gap could be almost wholly acco...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Vascular smooth muscle cells (SMCs) migrate from the arterial media to the intima in the progression of atherosclerosis, and dysfunction of SMCs leads to enhanced atherogenesis. A soluble form of the LDL receptor relative with 11 ligand-binding repeats (sLR11) is produced by the intimal SMCs, and the circula...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2009.127027
更新日期:2009-10-01 00:00:00
abstract::Hemostasis is initiated by injury to the vascular wall, leading to the deposition of platelets adhering to components of the subendothelium. Platelet adhesion requires the presence of von Willebrand factor and platelet receptors (IIb/IIIa and Ib/IX). Additional platelets are recruited to the site of injury by release ...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:2000-08-01 00:00:00
abstract::Problems encountered in the uroporphyrinogen I synthase assay were found to be due to the Triton reagent, slit-width and excitation wavelength settings of the spectrophotofluorometer, and leg-phase in the enzyme reaction. Of Triton X-100 from four suppliers, only three were found suitable. Differences in enzyme activi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::We have modified an automated measurement system of urinary iodine (UI) and established a sensitive UI assay system by using ultraviolet (UV) digestion. The automated system is sensitive enough to detect concentrations of UI < 0.78 mumol/L (< 10 micrograms/dL) in a small volume of urine (500 microL). Sample throughput...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::A highly sensitive two-site sandwich ELISA measuring total proinsulin immunoreactive material in serum or plasma was developed. The assay was based on two monoclonal antibodies, an anti-C-peptide antibody bound to a microtest plate and a biotin-labeled anti-insulin antibody. The detection limit (3 SD above zero value)...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::The most popular pretreatment method of plasma samples for the measurement of ascorbate (AsA) and dehydroascorbate (DHA) has been an acidic deproteinization via metaphosphoric acid or trichloroacetic acid. In general, DHA is absent in plasma samples prepared from human blood in a conventional manner. However, when the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract:BACKGROUND:Increased concentrations of cell-free DNA have been found in several disorders and have been interpreted as evidence of increased rates of cell death or turnover. Evidence from in vitro and animal experiments suggests that DNA may play a role in the pathogenesis of rheumatoid arthritis (RA). METHODS:We meas...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.084509
更新日期:2007-09-01 00:00:00
abstract::In this method for measuring amino acids in urine, serum, and tissue, the amino acids to be assayed and the internal standard, L-norleucine, are converted to phenylthiohydantoins , isolated by organic solvent extraction, separated by reversed-phase "high-pressure" liquid chromatography, and detected by ultraviolet abs...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::Determination of the ratio of testosterone to epitestosterone (T/E) in urine is used to detect testosterone administration in athletes, with a ratio > 6 considered as evidence of an offense. We show that administration of ketoconazole, which inhibits testosterone biosynthesis, may be useful for differentiating between...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Measurement of alpha-fetoprotein concentration and acetylcholinesterase activity in amniotic fluid can be used to identify chromosomal defects as well as neural tube defects. In seven cases of trisomy 21 and one case of partial trisomy 3, alpha-fetoprotein concentrations were below the reference range but values for a...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract:BACKGROUND:HDL is quantitatively the most important lipoprotein in most species and mechanistic evidence points toward a role for HDL in normal immune function. We tested the hypothesis that concentrations of HDL cholesterol are associated with risk of autoimmune disease. METHODS:From 2 studies of the general populati...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2018.299636
更新日期:2019-05-01 00:00:00
abstract::We report the adaptation of four radioassays to the prototype of an automated radioimmunoassay system ("Centria," Union Carbide). The system consists of three integrated modules: (a) an automated pipettor, which dispenses samples and reagents; (b) the key module, an incubator/separator, in which centrifugal force is u...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract:BACKGROUND:Although lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) levels are associated with cardiovascular events, Lp-PLA(2) is physically linked to LDL cholesterol (LDL-C). Whether measures of Lp-PLA(2) mass or activity continue to predict risk after LDL-C reduction by statin therapy is uncertain. METHODS:Lp...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2011.180281
更新日期:2012-05-01 00:00:00
abstract::We compared five ion-selective electrodes (ISEs; AVL 985S, Baxter Lytening 2Z, Beckman EL-ISE, Ciba-Corning 654 Na/K/Li, and Nova CRT 11) and a colorimetric method (Ektachem) for determination of lithium with flame atomic absorption and atomic emission spectroscopy. We evaluated precision, recovery, interference by dr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00