Abstract:
:V-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) is a member of CRK family and act as an adaptor protein participating in intra-cellular signal transduction. The role of CRKL in gastric cancer (GC) remains unclear. In this study, we show that CRKL was aberrantly highly expressed in both GC tumor specimens and cell lines (SGC-7901, MKN-45, MKN-28 and SUN-16). The expression of CRKL was significantly correlated with GC clinicopathologic features including tumor size, local invasion, lymph node metastasis and TNM stages. Knock-down of CRKL in SGC-7901 cells induced a suppression of cell proliferation along with a significant arrest of cell cycle in G0/G1 phase, however, no significant influence was observed on cell apoptosis. We validate that miR-126, a suppressor in GC, was a negative regulator of CRKL by directly combining with the 3' untranslated region of CRKL mRNA, and over-expression of miR-126 inhibited the protein expression of CRKL significantly. These results suggest that CRKL may function as an oncogene in GC by promoting the GC cell proliferation, which provides us a likely biomarker and a potential target for GC prevention, diagnosis and therapeutic treatment. Moreover, the targeting relationship between CRKL and miR-126 partly reveals the mechanism of miR-126 on GC suppression.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Wang J,Chen X,Li P,Su L,Yu B,Cai Q,Li J,Yu Y,Liu B,Zhu Zdoi
10.1016/j.cbi.2013.09.003subject
Has Abstractpub_date
2013-11-25 00:00:00pages
230-8issue
2eissn
0009-2797issn
1872-7786pii
S0009-2797(13)00228-7journal_volume
206pub_type
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journal_title:Chemico-biological interactions
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