Synaptic transmission at single visualized hippocampal boutons.

Abstract:

:We have used a focal stimulation method to study neurotransmission at synapses onto hippocampal pyramidal neurons in cultures derived from neonatal rats. Single functional boutons were visualized by activity-dependent preloading with the fluorescent dye FM1-43, then focally stimulated by localized application of elevated K+/Ca2+ solution via a puffer pipette, while postsynaptic currents were recorded under whole cell voltage clamp (Liu and Tsien, 1995). This paper gives a detailed description of the main properties of this experimental system and of information it has provided about fundamental properties of hippocampal synapses. Most of the experiments focused on excitatory postsynaptic currents (EPSCs), but preliminary recordings of inhibitory events (IPSCs) are also reported here. The unitary EPSCs at individual synapses varied greatly in amplitude, but were relatively uniform in their time course. The frequency of the synaptic events was greatly reduced by lowering the external Ca2+ concentration or by application of baclofen, a GABAB receptor agonist. Frequent repetitive stimulation produced a decline in the incidence of EPSCs that was readily reversed upon rest. We attribute the decline to exhaustion of a pool of available vesicles; typically, recovery proceeded with a time constant of approximately 40 sec (23 degrees C), and involved a vesicular pool capable of generating approximately 90 EPSCs without recycling. While synaptic currents were broadly distributed in amplitude (Bekkers et al., 1990), this distribution was remarkably similar at multiple synapses on a given postsynaptic neuron. The median synaptic current amplitude varied 4-fold across different cells, decreasing markedly with increasingly dense synaptic innervation. The implications of these results for cellular signal processing and quantal analysis are discussed.

journal_name

Neuropharmacology

journal_title

Neuropharmacology

authors

Liu G,Tsien RW

doi

10.1016/0028-3908(95)00143-t

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

1407-21

issue

11

eissn

0028-3908

issn

1873-7064

pii

002839089500143T

journal_volume

34

pub_type

杂志文章
  • Cluster formation of alpha7-containing nicotinic receptors at interneuronal interfaces in cell culture.

    abstract::Nicotinic receptors containing the alpha7 gene product are among the most abundant in the nervous system. Because of their widespread distribution and high relative permeability to calcium, the receptors regulate a diverse array of cellular events. On chick ciliary neurons the receptors are concentrated on somatic spi...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/s0028-3908(00)00132-5

    authors: Conroy WG,Ogden LF,Berg DK

    更新日期:2000-10-01 00:00:00

  • Central serotonin(2B) receptor blockade inhibits cocaine-induced hyperlocomotion independently of changes of subcortical dopamine outflow.

    abstract::The central serotonin2B receptor (5-HT2BR) is currently considered as an interesting pharmacological target for improved treatment of drug addiction. In the present study, we assessed the effect of two selective 5-HT2BR antagonists, RS 127445 and LY 266097, on cocaine-induced hyperlocomotion and dopamine (DA) outflow ...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2015.06.012

    authors: Devroye C,Cathala A,Di Marco B,Caraci F,Drago F,Piazza PV,Spampinato U

    更新日期:2015-10-01 00:00:00

  • Serotonin modulation of cell excitability and of [3H]GABA and [3H]D-aspartate efflux in primary cultures of rat cortical neurons.

    abstract::The effects of 5-hydroxytryptamine (5-HT) on neuronal excitability, evaluated as depolarization-induced firing rate, and on amino acid release, measured as electrically-evoked [(3)H]GABA and [(3)H]d-aspartate efflux, were investigated in rat primary cortical neuronal cultures. 5-HT displayed a concentration-dependent,...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2006.10.017

    authors: Bianchi C,Marani L,Marino S,Barbieri M,Nazzaro C,Beani L,Siniscalchi A

    更新日期:2007-03-01 00:00:00

  • Shifting towards a model of mGluR5 dysregulation in schizophrenia: Consequences for future schizophrenia treatment.

    abstract::Metabotropic glutamate receptor subtype 5 (mGluR5), encoded by the GRM5 gene, represents a compelling novel drug target for the treatment of schizophrenia. mGluR5 is a postsynaptic G-protein coupled glutamate receptor strongly linked with several critical cellular processes that are reported to be disrupted in schizop...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2015.08.003

    authors: Matosin N,Fernandez-Enright F,Lum JS,Newell KA

    更新日期:2017-03-15 00:00:00

  • CB1 receptor blockade reduces the anxiogenic-like response and ameliorates the neurochemical imbalances associated with alcohol withdrawal in rats.

    abstract::There is strong evidence that blocking CB1 receptors may reduce alcohol intake in alcohol-dependent individuals. However, there is still limited evidence that CB1 receptor antagonists may also be beneficial in the attenuation of alcohol withdrawal syndrome, even though alcohol withdrawal appears to be milder in CB1 re...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2008.02.005

    authors: Rubio M,Fernández-Ruiz J,de Miguel R,Maestro B,Michael Walker J,Ramos JA

    更新日期:2008-05-01 00:00:00

  • Involvement of hippocampal CA3 K(ATP) channels in contextual memory.

    abstract::This paper evaluates the involvement of hippocampal ATP-sensitive potassium channels (K(ATP)) in learning and memory. After confirming expression of the Kir6.2 subunit in the CA3 region of C57BL/6J mice, we performed intra-hippocampal pharmacological injections of specific openers and blockers of K(ATP) channels. The ...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2008.11.001

    authors: Betourne A,Bertholet AM,Labroue E,Halley H,Sun HS,Lorsignol A,Feng ZP,French RJ,Penicaud L,Lassalle JM,Frances B

    更新日期:2009-03-01 00:00:00

  • Allosteric modulators induce distinct movements at the GABA-binding site interface of the GABA-A receptor.

    abstract::Benzodiazepines (BZDs) and barbiturates exert their CNS actions by binding to GABA-A receptors (GABARs). The structural mechanisms by which these drugs allosterically modulate GABAR function, to either enhance or inhibit GABA-gated current, are poorly understood. Here, we used the substituted cysteine accessibility me...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2010.11.009

    authors: Sancar F,Czajkowski C

    更新日期:2011-02-01 00:00:00

  • Nucleus accumbens melanin-concentrating hormone signaling promotes feeding in a sex-specific manner.

    abstract::Melanin-concentrating hormone (MCH) is an orexigenic neuropeptide produced in the lateral hypothalamus and zona incerta that increases food intake. The neuronal pathways and behavioral mechanisms mediating the orexigenic effects of MCH are poorly understood, as is the extent to which MCH-mediated feeding outcomes are ...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2020.108270

    authors: Terrill SJ,Subramanian KS,Lan R,Liu CM,Cortella AM,Noble EE,Kanoski SE

    更新日期:2020-11-01 00:00:00

  • Supraspinal vs spinal sites of the antinociceptive action of the subtype-selective NMDA antagonist ifenprodil.

    abstract::The N-methyl-D-aspartate (NMDA) antagonist ifenprodil and several structurally related compounds are highly selective for the NR2B-containing receptor subtype. This selectivity could provide an explanation for the reported difference of the analgesic and side-effect profile of ifenprodil-like compounds from other NMDA...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/s0028-3908(00)00148-9

    authors: Chizh BA,Reissmüller E,Schlütz H,Scheede M,Haase G,Englberger W

    更新日期:2001-01-01 00:00:00

  • Warmer preparation of hippocampal slices prevents synapse proliferation that might obscure LTP-related structural plasticity.

    abstract::The hippocampal slice is a popular model system in which to study the cellular properties of long-term potentiation (LTP). Synaptogenesis induced by exposure to ice-cold artificial cerebrospinal fluid (ACSF), however, raises the concern that morphological correlates of LTP might be obscured, especially in mature slice...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2006.06.020

    authors: Bourne JN,Kirov SA,Sorra KE,Harris KM

    更新日期:2007-01-01 00:00:00

  • Midazolam inhibits long-term potentiation through modulation of GABAA receptors.

    abstract::Benzodiazepine drugs (BZ) are used for anxiety, insomnia, and seizures. They worsen memory, especially in large doses, but the mechanism of this action is uncertain. In micromolar concentrations, benzodiazepines have been shown to reduce long-term potentiation (LTP), which could be a cellular basis for their amnesic a...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(95)00182-4

    authors: Evans MS,Viola-McCabe KE

    更新日期:1996-03-01 00:00:00

  • Cerebrospinal fluid diazepam binding inhibitor in depressed patients and normal controls.

    abstract::Diazepam binding inhibitor (DBI) is a neuromodulatory peptide for gamma-aminobutyric acid (GABA) neurotransmission. Levels of DBI in cerebrospinal fluid (CSF) were found to be elevated in depressed patients, when compared to age- and sex-matched normal controls. Levels of the peptide, corticotropin-releasing hormone (...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/s0028-3908(11)80014-6

    authors: Roy A

    更新日期:1991-12-01 00:00:00

  • Prokineticin system modulation as a new target to counteract the amyloid beta toxicity induced by glutamatergic alterations in an in vitro model of Alzheimer's disease.

    abstract::The accumulation of β-amyloid (Aβ) is one of the hallmarks of Alzheimer disease (AD). Beyond the inflammatory reactions promoted by Aβ, it has been demonstrated that the prokineticin (PK) system, composed of the chemokine prokineticin 2 (PK2) and its receptors, is involved in Aβ toxicity. In this study we have analyze...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2016.12.012

    authors: Caioli S,Severini C,Ciotti T,Florenzano F,Pimpinella D,Petrocchi Passeri P,Balboni G,Polisca P,Lattanzi R,Nisticò R,Negri L,Zona C

    更新日期:2017-04-01 00:00:00

  • Endocannabinoid modulation of dopamine neurotransmission.

    abstract::Dopamine (DA) is a major catecholamine neurotransmitter in the mammalian brain that controls neural circuits involved in the cognitive, emotional, and motor aspects of goal-directed behavior. Accordingly, perturbations in DA neurotransmission play a central role in several neuropsychiatric disorders. Somewhat surprisi...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2017.04.033

    authors: Covey DP,Mateo Y,Sulzer D,Cheer JF,Lovinger DM

    更新日期:2017-09-15 00:00:00

  • N-acetylcysteine treatment blocks the development of ethanol-induced behavioural sensitization and related ΔFosB alterations.

    abstract::Ethanol addiction is a serious public health problem that still needs more effective pharmacological treatment. A key factor in the development and maintenance of this disease is the advent of neuroadaptations in the mesocorticolimbic brain pathway upon chronic ethanol abuse. In general, these neuroadaptations are mal...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2016.07.009

    authors: Morais-Silva G,Alves GC,Marin MT

    更新日期:2016-11-01 00:00:00

  • The influence of G protein subtype on agonist action at D2 dopamine receptors.

    abstract::In previous studies, we have shown that agonists influence the ability of D2 dopamine receptors to couple to G proteins and here we extend this work. The human D2Short dopamine receptor and a natural polymorphism of this D(2Short)(Ser311Cys), have been studied by co-expressing the receptors in insect cells with Gbeta1...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2004.06.010

    authors: Nickolls SA,Strange PG

    更新日期:2004-11-01 00:00:00

  • Prenatal treatment with methylazoxymethanol acetate as a neurodevelopmental disruption model of schizophrenia in mice.

    abstract::Methylazoxymethanol (MAM)-treated pregnant rat at gestation day (GD) 17 has been shown to be a valuable developmental animal model for schizophrenia. Yet, this model remains to be established in mice. In the present study, we examined behavioral, cytoarchitectural, and neurochemical changes in the offspring of MAM-tre...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2019.02.034

    authors: Takahashi K,Nakagawasai O,Sakuma W,Nemoto W,Odaira T,Lin JR,Onogi H,Srivastava LK,Tan-No K

    更新日期:2019-05-15 00:00:00

  • A study of time- and sex-dependent effects of vortioxetine on rat sexual behavior: Possible roles of direct receptor modulation.

    abstract::Treatment-related sexual dysfunction is a common side effect of antidepressants and contributes to patient non-compliance or treatment cessation. However, the multimodal antidepressant, vortioxetine, demonstrates low sexual side effects in depressed patients. To investigate the mechanisms involved, sexual behavior was...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2017.04.017

    authors: Li Y,Pehrson AL,Oosting RS,Gulinello M,Olivier B,Sanchez C

    更新日期:2017-07-15 00:00:00

  • Genetics of attention-deficit hyperactivity disorder (ADHD).

    abstract::Attention-deficit hyperactivity disorder (ADHD) is a clinically and genetically heterogeneous syndrome which is comorbid with childhood conduct disorder, alcoholism, substance abuse, dis-social personality disorder, and affective disorders. A small but consistent overlap with autistic symptoms has also been establishe...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2009.08.011

    authors: Sharp SI,McQuillin A,Gurling HM

    更新日期:2009-12-01 00:00:00

  • PSD-95 deficiency alters GABAergic inhibition in the prefrontal cortex.

    abstract::Postsynaptic Density Protein-95 (PSD-95) is a major scaffolding protein in the excitatory synapses in the brain and a critical regulator of synaptic maturation for NMDA and AMPA receptors. PSD-95 deficiency has been linked to cognitive and learning deficits implicated in neurodevelopmental disorders such as autism and...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2020.108277

    authors: McEachern EP,Coley AA,Yang SS,Gao WJ

    更新日期:2020-11-15 00:00:00

  • Neurotensin in reward processes.

    abstract::Neurotensin (NTS) is a neuropeptide neurotransmitter expressed in the central and peripheral nervous systems. Many studies over the years have revealed a number of roles for this neuropeptide in body temperature regulation, feeding, analgesia, ethanol sensitivity, psychosis, substance use, and pain. This review provid...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2020.108005

    authors: Torruella-Suárez ML,McElligott ZA

    更新日期:2020-05-01 00:00:00

  • Nicotinic receptors in non-human primates: Analysis of genetic and functional conservation with humans.

    abstract::Nicotinic acetylcholine receptors (nAChRs) are highly conserved between humans and non-human primates. Conservation exists at the level of genomic structure, protein structure and epigenetics. Overall homology of nAChRs at the protein level is 98% in macaques versus 89% in mice, which is highly relevant for evaluating...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2015.01.023

    authors: Shorey-Kendrick LE,Ford MM,Allen DC,Kuryatov A,Lindstrom J,Wilhelm L,Grant KA,Spindel ER

    更新日期:2015-09-01 00:00:00

  • Pharmacological characterization of serotonin 5-HT3 receptor-mediated electrical response in cultured mouse neuroblastoma cells.

    abstract::The aim of this study was to investigate the pharmacological characteristics of the 5-hydroxytryptamine-(5-HT)-induced electrical response in cultured neuroblastoma N1E-115 cells of the mouse. In these cells 5-HT induces a transient membrane depolarization, which is associated with a transient inward current, that has...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(88)90048-2

    authors: Neijt HC,te Duits IJ,Vijverberg HP

    更新日期:1988-03-01 00:00:00

  • Modulation of release of acetylcholine from the striatum by a proposed excitatory amino acid antagonist U-54494A: comparison with known antagonists, diazepam and phenytoin.

    abstract::The effect of (U-54494A) cis-3,4-dichloro-N-methyl-N-[2-(1-Pyrrolidinyl)- cyclohexyl] benzamide monohydrochloride, an excitatory amino acid antagonist, on N-methyl-D-aspartic acid (NMDA)- and K(+)-evoked release of [3H]acetylcholine [( 3H]ACh) from slices of striatum was investigated. For the purpose of comparison, MK...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(92)90019-l

    authors: Sethy VH,Sage GP

    更新日期:1992-02-01 00:00:00

  • Phylogenetic tree analysis of G protein-coupled 5-HT receptors: implications for receptor nomenclature.

    abstract::The nomenclature system used to characterize 5-hydroxytryptamine receptor subtypes remains controversial. To date, the majority of nomenclature systems have been on the differential pharmacological properties of the receptors. However, the availability of molecular biological data allows for a nomenclature system base...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(92)90138-f

    authors: Peroutka SJ

    更新日期:1992-07-01 00:00:00

  • Intermittent streptozotocin administration induces behavioral and pathological features relevant to Alzheimer's disease and vascular dementia.

    abstract:RATIONALE:Diabetes mellitus (DM) is a major risk factor for Alzheimer's disease and vascular dementia. Few animal models exist that focus on the metabolic contributions to dementia onset and progression. Thus, there is strong scientific rationale to explore the effects of streptozotocin (STZ), a diabetogenic compound, ...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2018.04.021

    authors: Murtishaw AS,Heaney CF,Bolton MM,Belmonte KCD,Langhardt MA,Kinney JW

    更新日期:2018-07-15 00:00:00

  • Pharmacological treatment of tic disorders and Tourette Syndrome.

    abstract::The present review gives an overview of current pharmacological treatment options of tic disorders and Tourette Syndrome (TS). After a short summary on phenomenology, clinical course and comorbid conditions we review indications for pharmacological treatment in detail. Unfortunately, standardized and large enough drug...

    journal_title:Neuropharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuropharm.2012.05.043

    authors: Roessner V,Schoenefeld K,Buse J,Bender S,Ehrlich S,Münchau A

    更新日期:2013-05-01 00:00:00

  • Effects of lead in the laboratory mouse--2. Development and social behaviour after lifelong administration of a small dose of lead acetate in drinking fluid.

    abstract::Administration of 0.13% lead acetate solution as drinking fluid to breeding mice was without significant effect on the numbers of live or dead births or on weight of pups at 1 day. The appearance of fur was delayed and the body weights at 21 days and 31-38 weeks were smaller than in controls. On weaning, the treated o...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(86)90036-5

    authors: Donald JM,Cutler MG,Moore MR,Bradley M

    更新日期:1986-02-01 00:00:00

  • The GABAergic Gudden's dorsal tegmental nucleus: A new relay for serotonergic regulation of sleep-wake behavior in the mouse.

    abstract::Serotonin (5-HT) neurons are involved in wake promotion and exert a strong inhibitory influence on rapid eye movement (REM) sleep. Such effects have been ascribed, at least in part to the action of 5-HT at post-synaptic 5-HT1A receptors (5-HT1AR) in the brainstem, a major wake/REM sleep regulatory center. However, the...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/j.neuropharm.2018.06.014

    authors: Chazalon M,Dumas S,Bernard JF,Sahly I,Tronche F,de Kerchove d'Exaerde A,Hamon M,Adrien J,Fabre V,Bonnavion P

    更新日期:2018-08-01 00:00:00

  • Intracerebroventricular injection of a nitric oxide synthase inhibitor does not affect long-term slope potentiation in vivo.

    abstract::Although there is evidence from in vitro studies to suggest that NO synthesis may be involved in the induction of hippocampal LTP, other in vitro studies and experiments conducted in vivo have provided conflicting results. In agreement with previous work conducted in this laboratory using an i.p. route of administrati...

    journal_title:Neuropharmacology

    pub_type: 杂志文章

    doi:10.1016/0028-3908(94)90040-x

    authors: Bannerman DM,Butcher SP,Morris RG

    更新日期:1994-11-01 00:00:00