Expanding room for tetrazine ligations in the in vivo chemistry toolbox.

abstract：:Inhibition of the steroid 5alpha-reductases shows promise in the treatment of a number of androgen-dependent disorders, such as benign prostatic hyperplasia, male pattern baldness, and acne. The design of potent and isozyme-selective inhibitors has provided biologists and clinicians with important tools for elucidatin...

journal_title：Current opinion in chemical biology

authors： Harris GS,Kozarich JW

更新日期：1997-08-01 00:00:00

abstract：:The principles of enzyme chemistry, mechanism of action and inhibitor design are being applied to proteomics by the development of activity-based probes. This approach suggests a potentially broad method for interrogating enzyme family members, both known and unknown, in cells and proteomic fractions without the need ...

journal_title：Current opinion in chemical biology

authors： Kozarich JW

更新日期：2003-02-01 00:00:00

abstract：:Conceptual and computational advances triggered an explosion of designed protein structures in the recent years. Various protein fold geometries have been robustly designed with atomic accuracy, including protein folds unseen in nature. The same principles and tools have been extended to design multi-chain assemblies....

journal_title：Current opinion in chemical biology

authors： Ljubetič A,Gradišar H,Jerala R

更新日期：2017-10-01 00:00:00

abstract：:Structural modification of naturally occurring beta-lactams and beta-lactones is a highly effective strategy for generating drugs for treating bacterial infections, cancer, obesity, and hyperlipidemia. These drugs acylate catalytic amino acids (serine, threonine, or cysteine) in enzyme targets such as penicillin-bindi...

journal_title：Current opinion in chemical biology

authors： Kluge AF,Petter RC

更新日期：2010-06-01 00:00:00

abstract：:Natural products are evolutionarily designed and chemically distinct from most synthetic library molecules. In addition to their role as drugs, they are successfully used as molecular probes to identify disease relevant targets. Novel natural products are still routinely discovered from traditional sources through cul...

journal_title：Current opinion in chemical biology

authors： Schmitt EK,Moore CM,Krastel P,Petersen F

更新日期：2011-08-01 00:00:00

abstract：:Simple surfactants achieve remarkable long-range order in aqueous environments. This organizing potential is seen most dramatically in biological membranes where phospholipid assemblies both define cell boundaries and provide a ubiquitous structural scaffold for controlling cellular chemistry. Here we consider simple ...

journal_title：Current opinion in chemical biology

authors： Childers WS,Ni R,Mehta AK,Lynn DG

更新日期：2009-12-01 00:00:00

abstract：:The appearance of the highly pathogenic avian influenza virus H5N1 highlighted the potential impact of influenza virus on humanity. The emergence of this high profile virus stimulated much research towards a better understanding of the key determinants for successful human-to-human transmission and as such has provide...

journal_title：Current opinion in chemical biology

authors： von Itzstein M

更新日期：2008-02-01 00:00:00

abstract：:The global nitrogen cycle is the process in which different forms of environmental N are interconverted by microorganisms either for assimilation into biomass or in respiratory energy-generating pathways. This short review highlights developments over the last 5 years in our understanding of functionality of nitrogena...

journal_title：Current opinion in chemical biology

authors： Eady RR,Antonyuk SV,Hasnain SS

更新日期：2016-04-01 00:00:00

abstract：:NMR, already some 50 years old, has long been an invaluable analytical method in industry for verification of chemical synthesis and compound characterisation. The range of molecular information accessible through NMR, however, offers a far larger horizon of applications. Of these, ligand screening by NMR has emerged ...

journal_title：Current opinion in chemical biology

authors： Diercks T,Coles M,Kessler H

更新日期：2001-06-01 00:00:00

abstract：:Compartmentalization is one of the defining features of life. Cells use protein compartments to exert spatial control over their metabolism, store nutrients and create unique microenvironments needed for essential physiological processes. Encapsulins are a recently discovered class of protein nanocompartments found in...

journal_title：Current opinion in chemical biology

authors： Giessen TW

更新日期：2016-10-01 00:00:00

abstract：:Exacerbation of climate change and air pollution around the world have emphasized the necessity of replacing fossil fuels with clean and sustainable energy. Metabolic engineering has provided strategies to engineer diverse organisms for the production of biofuels from renewable carbon sources. Although some of the pro...

journal_title：Current opinion in chemical biology

authors： Choi KR,Jiao S,Lee SY

更新日期：2020-12-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) genotyping has become a key technology for genetic studies. In recent years, matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry has emerged as a very powerful method for SNP genotyping. Here, we discuss our experience in implementing a high-through...

journal_title：Current opinion in chemical biology

authors： Lechner D,Lathrop GM,Gut IG

更新日期：2002-02-01 00:00:00

abstract：:The field of enzymology has long used small-molecule mass spectrometry. However, the direct interrogation of covalent and non-covalent intermediates by large-molecule mass spectrometry of enzymes or large peptide substrates is illuminating an increasingly diverse array of chemistries used in nature. Recent advances no...

journal_title：Current opinion in chemical biology

authors： Kelleher NL,Hicks LM

更新日期：2005-10-01 00:00:00

abstract：:Lipopolysaccharide (LPS), a glycolipid found in the outer membrane of Gram-negative bacteria, is a potent elicitor of innate immune responses in mammals. A typical LPS molecule is composed of three different structural domains: a polysaccharide called the O-antigen, a core oligosaccharide, and Lipid A. Lipid A is the ...

journal_title：Current opinion in chemical biology

authors： Xiao X,Sankaranarayanan K,Khosla C

更新日期：2017-10-01 00:00:00

abstract：:New insights have been gained into the formation of the di-iron tyrosyl radical cofactor, which is essential for nucleotide reduction in the class I ribonucleotide reductases. Recent advances include Insight into the function of the tyrosyl radical in initiation of nucleotide reduction. ...

journal_title：Current opinion in chemical biology

authors： Stubbe J

更新日期：2003-04-01 00:00:00

abstract：:Activation of unactivated C-H bonds is an important process in nature and organic synthesis. Nature employs a wide range of metalloproteins to efficiently catalyze such reactions. Quantum chemistry can be used to explore the reactivity of such systems. Atomic level insight into the catalytic mechanisms can be gained t...

journal_title：Current opinion in chemical biology

authors： Ye S,Neese F

更新日期：2009-02-01 00:00:00

abstract：:A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

journal_title：Current opinion in chemical biology

authors： Apweiler R,Bairoch A,Wu CH

更新日期：2004-02-01 00:00:00

abstract：:Inspired by advances in the ability to construct programmable circuits in living organisms, in vitro circuits are emerging as a viable platform for designing, understanding, and exploiting dynamic biochemical circuitry. In vitro systems allow researchers to directly access and manipulate biomolecular parts without the...

journal_title：Current opinion in chemical biology

authors： Hockenberry AJ,Jewett MC

更新日期：2012-08-01 00:00:00

abstract：:Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

journal_title：Current opinion in chemical biology

authors： Stein LY

更新日期：2019-04-01 00:00:00

abstract：:Because biomarkers are typically low in abundance, the crucial step of biomarker discovery is to efficiently separate clinically relevant sets of proteins that might define disease stages and/or predict disease development. It is anticipated that a multi-dimensional fractionation system (MDFS) will provide an efficien...

journal_title：Current opinion in chemical biology

authors： Lee HJ,Lee EY,Kwon MS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:Due to the existence of numerous isomers, the in-depth analysis of glycans represents a major challenge. Currently, the majority of glycans are analysed using mass spectrometry (MS)-based techniques, which can provide information on regioisomers but usually fail to differentiate stereoisomers. A promising approach to ...

journal_title：Current opinion in chemical biology

authors： Manz C,Pagel K

更新日期：2018-02-01 00:00:00

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00

abstract：:Methanogenesis from trimethylamine, dimethylamine or monomethylamine is initiated by a series of corrinoid-dependent methyltransferases. The non-homologous genes encoding the full-length methyltransferases each possess an in-frame UAG (amber) codon that does not terminate translation. The amber codon is decoded by a d...

journal_title：Current opinion in chemical biology

authors： Krzycki JA

更新日期：2004-10-01 00:00:00

abstract：:A number of nitrile-related enzymes have been screened over the past year for use in synthetic applications. There have also been significant advances in our understanding of the structures and modes of regulation of metal-containing nitrile hydratases. Enzyme structural characterization has provided new insights into...

journal_title：Current opinion in chemical biology

authors： Kobayashi M,Shimizu S

更新日期：2000-02-01 00:00:00

abstract：:Significant advances have been made over the past decade on illustrating structural and functional features underneath the selective metal ion recognition by various proteins in nature. These efforts led to fruitful information regarding mechanisms that bacteria employed on the regulation, transportation, and utilizat...

journal_title：Current opinion in chemical biology

authors： Chen PR,He C

更新日期：2008-04-01 00:00:00

abstract：:The catalytic pathways of soluble methane monooxygenase (sMMO) and cytochrome P450CAM, iron-containing enzymes, are described and compared. Recent extensive density functional ab initio electronic structure calculations have revealed many similarities in a number of the key catalytic steps, as well as some important d...

journal_title：Current opinion in chemical biology

authors： Guallar V,Gherman BF,Lippard SJ,Friesner RA

更新日期：2002-04-01 00:00:00

abstract：:Medicinal chemistry principles are being increasingly applied to the design of smaller, high purity, information-rich libraries. Recent computational advances in statistical methodology, the design of libraries to reduce ADMET problems, targeting protein families and revisiting natural products as sources of inspirati...

journal_title：Current opinion in chemical biology

authors： Rose S,Stevens A

更新日期：2003-06-01 00:00:00

abstract：:Natural enzymes that catalyze Diels-Alder reactions have long been sought after, yet few enzymes have been experimentally confirmed to perform this reaction. In the past five years, several stand-alone enzymes that can catalyze the Diels-Alder reaction had been identified and characterized. Among which, the crystal st...

journal_title：Current opinion in chemical biology

authors： Hashimoto T,Kuzuyama T

更新日期：2016-12-01 00:00:00

abstract：:Synthetic biology offers a bottom-up engineering approach that intends to understand complex systems via design-build-test cycles. Embryonic development comprises complex processes that originate at the level of gene regulatory networks in a cell and emerge into collective cellular behaviors with multicellular forms a...

journal_title：Current opinion in chemical biology

authors： Ebrahimkhani MR,Ebisuya M

更新日期：2019-10-01 00:00:00