当前位置： SCI文献检索 > EUROPEAN JOURNAL OF ENDOCRINOLOGY期刊下所有文献 > Evidence for a role for insulin-like growth factor binding proteins in glucocorticoid inhibition of normal human osteoblast-like cell proliferation.

Evidence for a role for insulin-like growth factor binding proteins in glucocorticoid inhibition of normal human osteoblast-like cell proliferation.

Abstract：

:Glucocorticoids (GCs) inhibit bone formation in vivo and inhibit osteoblast proliferation and collagen synthesis in vitro. These effects may be mediated by alterations in the insulin-like growth factor (IGF) system. In the present study of normal human osteoblast-like (HOB) cells, we tested the hypothesis that dexamethasone (Dex) inhibits IGF anabolic activity in bone by altering expression of IGF binding proteins (IGFBPs), particularly by decreasing expression of IGFBP-5 and IGFBP-3 (which enhance IGF activity) and increasing expression of IGFBP-4 (which inhibits IGF actions). Dexamethasone treatment caused a dose-dependent inhibition of HOB cell proliferation (69 +/- 4% of control at 10(-8) mol/l Dex) in seven separate experiments. Dexamethasone decreased IGFBP-5 mRNA levels to 20-30% of control (10(-8) and 10(-7) mol/l for 24 h). In six of six HOB preparations, 10(-8) mol/l Dex decreased IGFBP-5 mRNA levels (35 +/- 7% of control) and this effect was time dependent. Dexamethasone also decreased IGFBP-3 mRNA levels (74 +/- 9% of control in three HOB preparations). Dexamethasone decreased secretion of 29-31-kD IGFBP-5 and 38-42-kD IGFBP-3 proteins, determined by Western ligand blot and IGFBP-5 immunoblot, and induced a dose-dependent decrease in IGFBP-3 and IGFBP-5 secretion determined by specific radioimmunoassays. The effects of Dex on IGFBP-4 mRNA and on secretion of 25-kD IGFBP-4 levels were inconsistent between different cell preparations. Results suggest that GC inhibition of IGFBP-5 and IGFBP-3 production could decrease IGF activities and contribute to GC inhibition of bone formation.

journal_name

Eur J Endocrinol

journal_title

European journal of endocrinology

authors

Chevalley T,Strong DD,Mohan S,Baylink D,Linkhart TA

doi

10.1530/eje.0.1340591

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

591-601

issue

5

eissn

0804-4643

issn

1479-683X

journal_volume

134

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF ENDOCRINOLOGY文献大全
  • Bioelectrical impedance assessment of body composition in thyroid disease.

    abstract：:To assess the metabolic effects of thyroid disease, body composition was determined by bioimpedance analysis (BIA) in 72 patients with untreated hyperthyroidism (mean age 48.7 +/- 1.9 years) and 26 patients with untreated hypothyroidism (63.8 +/- 3.4 years). Bioelectrical whole body resistance (R) and reactance (Xc) w...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1360493

    authors： Seppel T,Kosel A,Schlaghecke R

    更新日期：1997-05-01 00:00:00

  • Pharmacokinetics and tolerability of a bioadhesive buccal testosterone tablet in hypogonadal men.

    abstract：OBJECTIVE:A phase I single centre, open label study of the pharmacokinetics and tolerability of a buccal testosterone tablet (COL 1621) was carried out. DESIGN:Twelve testosterone-deficient males were treated with the buccal tablet twice daily for 7 consecutive days. Multiple blood samples were drawn for testosterone,...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章

    doi：10.1530/eje.0.1500057

    authors： Ross RJ,Jabbar A,Jones TH,Roberts B,Dunkley K,Hall J,Long A,Levine H,Cullen DR

    更新日期：2004-01-01 00:00:00

  • Effect of insulin and growth hormone on the synthesis of radiolabelled proteoglycans from cultured human arterial smooth-muscle cells.

    abstract：:The present study focuses on the pathogenesis of increased frequency of large-vessel disease in diabetes. The diabetic arterial wall displays characteristic alterations of the extracellular matrix secreted by arterial smooth-muscle cells. The effects of insulin and growth hormone on the synthesis of sulphate-labelled ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1340326

    authors： Thøgersen VB,Heickendorff L,Ledet T

    更新日期：1996-03-01 00:00:00

  • Pregnancy and pituitary disorders.

    abstract：:Major hormonal changes emerge during pregnancy. The pituitary gland is one of the most affected organs with altered anatomy and physiology. The pituitary gland is enlarged as a result of lactotroph hyperplasia. Due to physiological changes in the pituitary and target hormone levels, binding globulins, and placental ho...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-09-0923

    authors： Karaca Z,Tanriverdi F,Unluhizarci K,Kelestimur F

    更新日期：2010-03-01 00:00:00

  • Genetic influence of an ACTH receptor promoter polymorphism on adrenal androgen secretion.

    abstract：OBJECTIVE:Adrenocorticotropic hormone (ACTH) is the primary secretagogue stimulating secretion of adrenal androgens (AA). Yet, genetic and environmental factors are assumed to play a determining role in the regulation of their biosynthesis and thus might explain the high variability of AA levels. Here we investigate th...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.1.02015

    authors： Reisch N,Slawik M,Zwermann O,Beuschlein F,Reincke M

    更新日期：2005-11-01 00:00:00

  • MRI T2 signal intensity and tumor response in patients with GH-secreting pituitary macroadenoma: PRIMARYS post-hoc analysis.

    abstract：:Objective Pituitary adenoma MRI T2 signal intensity associates with tumor characteristics including responsiveness to somatostatin analogs (SSAs). These analyses determined whether baseline T2 signal intensity predicts response to primary medical treatment with long-acting SSA. Design Post-hoc analyses of the prospect...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-18-0254

    authors： Bonneville F,Rivière LD,Petersenn S,Bevan J,Houchard A,Sert C,Caron PJ,PRIMARYS Study group.

    更新日期：2018-12-01 00:00:00

  • MECHANISMS IN ENDOCRINOLOGY: Anorexia nervosa and endocrinology: a clinical update.

    abstract：:Anorexia nervosa is a syndrome, that is collections of symptoms, which is not defined by its etiology. The severe cases are intractable. The syndrome is associated with multiple, profound endocrine alterations which may be adaptive, reactive or etiologic. Adaptive changes potentially may be inappropriate in clinical s...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-18-0596

    authors： Støving RK

    更新日期：2019-01-01 00:00:00

  • Cushing's syndrome: update on signs, symptoms and biochemical screening.

    abstract：:Endogenous pathologic hypercortisolism, or Cushing's syndrome, is associated with poor quality of life, morbidity, and increased mortality. Early diagnosis may mitigate against this natural history of the disorder. The clinical presentation of Cushing's syndrome varies, in part related to the extent and duration of co...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-15-0464

    authors： Nieman LK

    更新日期：2015-10-01 00:00:00

  • Advances in diagnostic practices affect thyroid cancer incidence in France.

    abstract：OBJECTIVE:To analyse trends in diagnostic practices of thyroid diseases and to relate them to the increase in thyroid cancer incidence in France over time. DESIGN:From 1980 to 2000, a French retrospective multicentric (three endocrinology and three nuclear medicine centres) study of thyroid diseases was conducted on 2...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,多中心研究

    doi：10.1530/eje.0.1500133

    authors： Leenhardt L,Bernier MO,Boin-Pineau MH,Conte Devolx B,Maréchaud R,Niccoli-Sire P,Nocaudie M,Orgiazzi J,Schlumberger M,Wémeau JL,Chérie-Challine L,De Vathaire F

    更新日期：2004-02-01 00:00:00

  • Identification and characterization of insulin-like growth factor I (IGF-I) and IGF-II/mannose-6-phosphate (IGF-II/M6P) receptors in bovine adrenal cells.

    abstract：:We have identified and characterized insulin-like growth factor I (IGF-I) and IGF-II/mannose-6-phosphate (IGF-II/M6P) receptors in bovine adrenal cells. Iodine-125-labeled IGF-I ([125I]IGF-I) binding was characteristic of the IGF-I receptor, and binding kinetics as well as receptor densities were similar in cortical a...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1300265

    authors： Weber MM,Kiess W,Beikler T,Simmler P,Reichel M,Adelmann B,Kessler U,Engelhardt D

    更新日期：1994-03-01 00:00:00

  • A perspective on stem cells by a clinician.

    abstract：:Stem cell terminology has entered the lexicon of medical practitioners even though the application of harvesting stem cells to treat diseases other than haematological disorders is not yet a reality in clinical practice. All branches of medicine will be affected by the new technology, more so those related to regenera...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.151u003

    authors： Hughes IA

    更新日期：2004-11-01 00:00:00

  • No relationship between identified variants in the uncoupling protein 2 gene and energy expenditure.

    abstract：OBJECTIVE:The uncoupling protein 2 (UCP2) uncouples respiration from the oxidative phosphorylation in most cell types, predominantly in white fat and skeletal muscle. Since a decreased basal metabolic rate (BMR) would increase the susceptibility to weight gain, genetic alterations in the UCP2 gene could contribute to t...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1390217

    authors： Klannemark M,Orho M,Groop L

    更新日期：1998-08-01 00:00:00

  • TSH-suppressive doses of levothyroxine are required to achieve preoperative native serum triiodothyronine levels in patients who have undergone total thyroidectomy.

    abstract：OBJECTIVE:Thyroidal production of triiodothyronine (T(3)) is absent in patients who have undergone total thyroidectomy. Therefore, relative T(3) deficiency may occur during postoperative levothyroxine (L-T(4)) therapy. The objective of this study was to evaluate how the individual serum T(3) level changes between preop...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-11-1029

    authors： Ito M,Miyauchi A,Morita S,Kudo T,Nishihara E,Kihara M,Takamura Y,Ito Y,Kobayashi K,Miya A,Kubota S,Amino N

    更新日期：2012-09-01 00:00:00

  • Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells.

    abstract：OBJECTIVE:To evaluate the effect of the growth factor activin A on the secretion of prolactin (PRL) and GH in cultured GH3 cells. METHODS:The concentrations of PRL and GH secreted from GH3 cells cultured in media with and without activin A were measured by RIA, and the expression of PRL mRNA and GH mRNA were analyzed ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1420506

    authors： Tamura N,Irahara M,Kuwahara A,Ushigoe K,Sugino H,Aono T

    更新日期：2000-05-01 00:00:00

  • Metabolic and endocrine effects of treatment with peroral or transdermal oestrogens in conjunction with peroral cyproterone acetate in women with polycystic ovary syndrome.

    abstract：OBJECTIVE:To compare the influence of transdermal and peroral oestrogen treatments in conjunction with cyproterone acetate (CPA) on metabolic and hormonal parameters in women with polycystic ovary syndrome (PCOS). PATIENTS AND METHODS:Twenty-four women with PCOS, aged 25.4+/-4.3 (mean+/-s.d.) years, body mass index 24...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1530/eje.0.1500215

    authors： Vrbíková J,Stanická S,Dvoráková K,Hill M,Vondra K,Bendlová B,Stárka L

    更新日期：2004-02-01 00:00:00

  • Sex hormone-binding globulin predicts the incidence of hyperglycemia in women: interactions with adiponectin levels.

    abstract：OBJECTIVE:Previous evidence has suggested that a low sex hormone-binding globulin (SHBG) concentration is associated with insulin-resistance and a low adiponectin concentration. We investigated the association between SHBG and the risk of hyperglycemia in each sex and we determined potential interactions between SHBG a...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-09-0202

    authors： Bonnet F,Balkau B,Malécot JM,Picard P,Lange C,Fumeron F,Aubert R,Raverot V,Déchaud H,Tichet J,Lecomte P,Pugeat M,DESIR Study Group.

    更新日期：2009-07-01 00:00:00

  • Imaging in endocrinology: 2-[18F]-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography in differentiated thyroid carcinoma: clinical indications and controversies in diagnosis and follow-up.

    abstract：:In recent years, 2-[(18)F]-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (FDG-PET/CT) has emerged as an important tool for the postoperative management of patients with differentiated thyroid cancer (DTC) and it is widely used in selected clinical situations. The most valuable role that FDG...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-15-0066

    authors： Salvatori M,Biondi B,Rufini V

    更新日期：2015-09-01 00:00:00

  • Polyglandular autoimmune syndromes.

    abstract：:The polyglandular autoimmune syndromes (PAS) comprise a wide spectrum of autoimmune disorders and are divided into a very rare juvenile (PAS type I) and a relatively common adult type with (PAS II) or without adrenal failure (PAS III). First clinical manifestation of PAS I usually occurs in childhood, whereas PAS II m...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-09-0044

    authors： Kahaly GJ

    更新日期：2009-07-01 00:00:00

  • Ectopic Agouti protein overexpression increases stimulated corticosterone production without effect on adenylate cyclase activity in mouse adrenal cells.

    abstract：OBJECTIVE:The antagonism of Agouti protein (AP) and Agouti-related protein on melanocortin receptors suggests an inhibitory role in the regulation of steroidogenesis. However, we have previously demonstrated that ectopic AP overexpression increased restraint-induced corticosterone release and adrenal reactivity to ACTH...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1510613

    authors： Shevchenko AY,Bazhan NM,Makarova EN,Yakovleva TV,Karkaeva NR

    更新日期：2004-11-01 00:00:00

  • Validity and clinical applicability of the acromegaly quality of life questionnaire, AcroQoL: a 6-month prospective study.

    abstract：OBJECTIVE:Validate the acromegaly quality of life (AcroQoL) questionnaire as a disease-generated questionnaire, which analyses physical and psychological domains, the latter subdivided into appearance and personal relationship sub-scales, to evaluate health-related quality of life (HRQoL) in acromegaly. DESIGN:Prospec...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,多中心研究

    doi：10.1530/eje.1.02214

    authors： Webb SM,Badia X,Surinach NL,Spanish AcroQol Study Group.

    更新日期：2006-08-01 00:00:00

  • Effect of chronic thyroxine treatment on IGF-I, IGF-II and IGF-binding protein expression in mammary gland and liver during pregnancy and early lactation in rats.

    abstract：OBJECTIVE:Hyperthyroidism in rats produces organ hypertrophy and increases in circulating IGF-I and IGF-binding protein (IGFBP)-3. Chronic treatment with thyroxine (T(4)) during pregnancy advances parturition, blocks lactation and changes several hormone receptors in mammary gland and liver. Since IGFs are implicated i...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1460729

    authors： Rosato R,Lindenbergh-Kortleve D,Neck Jv,Drop S,Jahn G

    更新日期：2002-05-01 00:00:00

  • Cold exposure inhibits leptin secretion in vitro by a direct and non-specific action on adipose tissue.

    abstract：OBJECTIVE:Leptin secretion is reduced by low temperatures in experimental animals, and this effect has been explained as an adaptive mechanism to cold environments. This study investigated the in vitro effects of cold exposure on human white adipose tissue. DESIGN:To understand whether the low temperature action is a ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1420195

    authors： Peinó R,Piñeiro V,Gualillo O,Menéndez C,Brenlla J,Casabiell X,Diéguez C,Casanueva FF

    更新日期：2000-02-01 00:00:00

  • Partial visual recovery from radiation-induced optic neuropathy after hyperbaric oxygen therapy in a patient with Cushing disease.

    abstract：:Here we describe the case of a 41-year-old woman with a history of Cushing disease who had previously undergone unsuccessful neurosurgery, followed by stereotactic radiosurgery. More than 4 years after this treatment, she presented severe visual impairment, which started in the left eye and was documented by neuro-oph...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.1.02161

    authors： Boschetti M,De Lucchi M,Giusti M,Spena C,Corallo G,Goglia U,Ceresola E,Resmini E,Vera L,Minuto F,Ferone D

    更新日期：2006-06-01 00:00:00

  • Long-acting somatostatin analogues are an effective treatment for type 1 gastric carcinoid tumours.

    abstract：BACKGROUND:Gastric carcinoid tumours type 1 (GCA1) originate from hyperplastic enterochromaffin-like (ECL) cells secondary to hypergastrinaemia. Treatment with somatostatin analogues (SSA) might impede ECL-cell hyperplasia by suppressing gastrin secretion and/or by a direct anti-proliferative effect on ECL cells. We co...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1530/EJE-08-0420

    authors： Grozinsky-Glasberg S,Kaltsas G,Gur C,Gal E,Thomas D,Fichman S,Alexandraki K,Barak D,Glaser B,Shimon I,Gross DJ

    更新日期：2008-10-01 00:00:00

  • Plasma methoxytyramine: clinical utility with metanephrines for diagnosis of pheochromocytoma and paraganglioma.

    abstract：CONTEXT:Measurements of plasma methoxytyramine, the O-methylated dopamine metabolite, are useful for detecting rare dopamine-producing pheochromocytomas and paragangliomas (PPGLs) and head and neck paragangliomas (HNPGLs), but utility for screening beyond that achieved using standard measurements of normetanephrine and...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,多中心研究

    doi：10.1530/EJE-17-0077

    authors： Rao D,Peitzsch M,Prejbisz A,Hanus K,Fassnacht M,Beuschlein F,Brugger C,Fliedner S,Langton K,Pamporaki C,Gudziol V,Stell A,Januszewicz A,Timmers HJLM,Lenders JWM,Eisenhofer G

    更新日期：2017-08-01 00:00:00

  • Giant prolactinomas in women.

    abstract：OBJECTIVE:To characterise distinctive clinical features of giant prolactinomas in women. DESIGN:A multicentre, retrospective case series and literature review. METHODS:We collected data from 15 female patients with a pituitary tumour larger than 4 cm and prolactin levels above 1000 μg/l and identified 19 similar case...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,多中心研究,评审

    doi：10.1530/EJE-13-0503

    authors： Delgrange E,Raverot G,Bex M,Burman P,Decoudier B,Devuyst F,Feldt-Rasmussen U,Andersen M,Maiter D

    更新日期：2013-11-22 00:00:00

  • Can we predict relapse in Graves' disease? Results from a systematic review and meta-analysis.

    abstract：BACKGROUND:Identification of pretreatment risk factors predicting relapse in patients with hyperthyroidism of Graves' disease after stopping anti-thyroid drugs (ATD) is decisive to guide therapeutic options. PURPOSE:We performed a systematic search and meta-analysis to study predictors for relapse after stopping ATD i...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1530/EJE-16-0725

    authors： Struja T,Fehlberg H,Kutz A,Guebelin L,Degen C,Mueller B,Schuetz P

    更新日期：2017-01-01 00:00:00

  • MANAGEMENT OF ENDOCRINE DISEASE: Novel anabolic treatments for osteoporosis.

    abstract：:Skeletal anabolic agents enhance bone formation, which is determined by the number and function of osteoblasts. Signals that influence the differentiation and function of cells of the osteoblast lineage play a role in the mechanism of action of anabolic agents in the skeleton. Wnts induce the differentiation of mesenc...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-17-0920

    authors： Canalis E

    更新日期：2018-02-01 00:00:00

  • Long-term treatment of acromegaly with the slow-release somatostatin analogue lanreotide.

    abstract：:Thirteen patients with active acromegaly despite previous surgery were treated with 30 mg lanreotide im twice a month for 9 months. In 10 subjects the treatment continued to 19 months. GH serum levels of all patients decreased significantly from an initial value of 32.0 (29.4) micrograms/l [median (standard error of m...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1310020

    authors： Marek J,Hána V,Krsek M,Justová V,Catus F,Thomas F

    更新日期：1994-07-01 00:00:00

  • Diagnostic and prognostic value of immunocytochemistry and BRAF mutation analysis on liquid-based biopsies of thyroid neoplasms suspicious for carcinoma.

    abstract：OBJECTIVE:In the field of fine-needle aspiration cytology, the category of suspicious for malignancy (SM) thyroid lesions, that bears 55-85% risk of malignant histology, is a challenging topic in which morphology alone is not always able to make a correct diagnosis. Recently, immunocytochemistry (ICC) has been referred...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-13-0023

    authors： Rossi ED,Martini M,Capodimonti S,Straccia P,Cenci T,Lombardi CP,Pontecorvi A,Larocca LM,Fadda G

    更新日期：2013-05-02 00:00:00