Abstract:
:Countercurrent centrifugal elutriation was used to separate growth plate chondrocytes from porcine basing on their differences in sizes and densities. Eighteen fractions of cells with different sizes and densities were obtained. The mean cellular volumes increased progressively in each of successive fractions, and that increase was associated with specific phenotypic changes, such as biochemical differences in DNA synthesis, proteoglycan synthesis, and activities of alkaline phosphatase. Three distinct chondrocyte subpopulations with their unique characteristics were identified among the elutriated fractions. The resting chondrocytes were found to be small in size and quiescent. The hypertrophic chondrocytes were found to be large in size and metabolically active both in alkaline phosphatase and in proteoglycan productions. The proliferative chondrocytes exhibited a high DNA synthesis rate, and their sizes were found to be between those of the resting and hypertrophic chondrocytes.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Lee KM,Fung KP,Leung PC,Leung KSdoi
10.1002/(sici)1097-4644(19960315)60:4<508::aid-jcbsubject
Has Abstractpub_date
1996-03-15 00:00:00pages
508-20issue
4eissn
0730-2312issn
1097-4644pii
10.1002/(SICI)1097-4644(19960315)60:4<508::AID-JCBjournal_volume
60pub_type
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