High-avidity anti-DNA antibody removal from the serum of systemic lupus erythematosus patients by adsorption using dextran sulfate cellulose columns.

Abstract:

:The purpose of this study is to determine whether immunoadsorption treatment using a dextran sulfate (DS) column can remove high-avidity anti-double-stranded DNA (anti-dsDNA) antibody from the blood of patients with systemic lupus erythematosus (SLE). Before and after each immunoadsorption therapy routine, titers of the high-avidity anti-dsDNA antibody of 11 SLE patients were measured by using a newly developed assay kit to exclusively detect high avidity anti-DNA antibody. Patients with active SLE showed significantly higher titers of high-avidity antibody than did those with inactive SLE, and their titers were significantly reduced by immunoadsorption procedures. Removal of high-avidity antibodies in vitro was also confirmed by mixing patients' sera and DS gel. Immunoadsorption therapy using DS columns is effective in the removal of high-avidity anti-dsDNA antibodies that are closely associated with pathogenicity in SLE.

journal_name

J Clin Apher

authors

Matsuki Y,Suzuki K,Kawakami M,Ishizuka T,Kawaguchi Y,Hidaka T,Nakamura H

doi

10.1002/(SICI)1098-1101(1996)11:1<30::AID-JCA7>3.0

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

30-5

issue

1

eissn

0733-2459

issn

1098-1101

pii

10.1002/(SICI)1098-1101(1996)11:1<30::AID-JCA7>3.0

journal_volume

11

pub_type

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