当前位置： SCI文献检索 > DOMESTIC ANIMAL ENDOCRINOLOGY期刊下所有文献 > Establishment of a reliable extraction method of estrone sulfate from bovine plasma for detection of the peripheral level during the regular estrous cycle by radioimmunoassay.

Establishment of a reliable extraction method of estrone sulfate from bovine plasma for detection of the peripheral level during the regular estrous cycle by radioimmunoassay.

Abstract：

:A simple extraction and assay technique of estrone sulfate in bovine blood was developed with the object of detecting the peripheral level of estrone sulfate in a normal estrous cycle or in early pregnancy. Estrone sulfate in bovine plasma was extracted with a small reversed phase cartridge. The steroid conjugate retained in the cartridge was eluted with 40% (v/v) methanol. Estrone sulfate was separately recovered from other steroids by the stepwise increase in methanol concentration in the elution solvent. The recoveries of estrone sulfate eluted with 40% methanol were more than 90%, irrespective of the applied plasma volume. The concentration measured by radioimmunoassay with the eluent of 40% methanol was consistent for plasma extraction volumes of 0.5-2.0 ml. The change of estrone sulfate in bovine peripheral plasma during the regular estrous cycle was determined with a small reversed phase cartridge for extraction and 40% methanol for elution. The change in estrone sulfate was found to be similar to the change of estrone and estradiol-17 beta. The concentration of estrone sulfate was not higher than that of both estrogens in cattle.

journal_name

Domest Anim Endocrinol

journal_title

Domestic animal endocrinology

authors

Hirako M,Takahashi H,Domeki I

doi

10.1016/0739-7240(96)00013-6

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

187-95

issue

3

eissn

0739-7240

issn

1879-0054

pii

0739-7240(96)00013-6

journal_volume

13

pub_type

杂志文章

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