Abstract:
:Using a mouse zosteriform model that mimics human herpes simplex virus (HSV) infection in several aspects, the effectiveness of plasmid DNA encoding the immediate early protein ICP 27 was evaluated as a vaccine. Animals were immunized intramuscularly twice with DNA, then either challenged with virus or killed, and the nature of the immune response induced was measured. After intramuscular injection with plasmid DNA encoding ICP 27 (pc-ICP 27), solid protection was evident in 70-80% of mice and the lesions were delayed in the remaining animals. Immune splenocytes obtained from pc-ICP 27 immune mice showed HSV-specific lymphoproliferation, MHC-class I restricted cytotoxic T-lymphocyte (CTL) activity, and type 1 cytokine production. These animals also exhibited delayed-type hypersensitivity (DTH) reactions. Adoptive transfer studies conducted on syngeneic nude mice revealed that those recipients of immune CD4+ T cells, but not CD8+ T cells, were protected from subsequent HSV-1 (strain 17) challenge. Thus pc-ICP 27 DNA immunization protected the mice principally by CD4+ T cells and it is likely that these cells were Th-1 type because only type 1 cytokines were detectable after in vitro antigen stimulation. Our results indicate the potential value of DNA encoding nonstructural viral proteins as vaccines against HSV.
journal_name
Viral Immunoljournal_title
Viral immunologyauthors
Manickan E,Yu Z,Rouse RJ,Wire WS,Rouse BTdoi
10.1089/vim.1995.8.53subject
Has Abstractpub_date
1995-01-01 00:00:00pages
53-61issue
2eissn
0882-8245issn
1557-8976journal_volume
8pub_type
杂志文章相关文献
VIRAL IMMUNOLOGY文献大全abstract::Abstract IL-17 producing CD4 T cells have recently been shown to play an important role in mucosal immunity in HIV infection. But its role in peripheral immunity and the molecular mechanism underlying its regulation during HIV-1 infection are ill defined. In this study, we report a significant negative correlation b...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2012.0048
更新日期:2013-02-01 00:00:00
abstract::A paresis isolate of equid herpesvirus 1 (EHV1, Ab4/8) and a plaque-purified virus derived from it (EHV1, Ab4/13), induced long-term suppression of both mitogenic and antigen-specific lymphocyte proliferations in adult outbred ponies. Peripheral blood mononuclear cells (PBMC) taken from a pony after EHV1 infection sup...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1999.12.313
更新日期:1999-01-01 00:00:00
abstract::TransFix(TM) and Cyto-Chex((R)) BCT (blood collection tube) reagents have been shown to maintain whole blood integrity for delayed immunophenotyping by flow cytometry. We evaluated the ability of these blood-stabilizing reagents to preserve HIV-seropositive blood for delayed CD4(+) T-cell quantification utilizing the ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2009.0027
更新日期:2009-10-01 00:00:00
abstract::Naïve CD8(+) T cells differentiate into effectors secreting various cytokines that modulate immune functions. A striking finding for most HIV-1-infected patients is that they accumulate CD8(+) T cells belonging to early and intermediate differentiated elements. Structural HIV-1 proteins, and among these the matrix pro...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2007.0095
更新日期:2008-06-01 00:00:00
abstract::We investigated the duration of humoral responses to dengue virus infection in individuals who recalled experiencing dengue fever-like illnesses at the time of the Second World War, when dengue fever epidemics occurred throughout the Pacific and Southeast Asia. In July 1943 dengue fever reappeared in Hawaii following ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2007.0050
更新日期:2007-12-01 00:00:00
abstract::CD8(+) T-cell-mediated pulmonary immunopathology in respiratory virus infection is mediated in large part by antigen-specific TNF-α expression by antiviral effector T cells, which results in epithelial chemokine expression and inflammatory infiltration of the lung. To further define the signaling events leading to lun...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2010.0076
更新日期:2010-12-01 00:00:00
abstract::Current treatments for hepatitis C infection have limited efficacy, and there is no vaccine available. The goal of this study was to compare the immune response to several immunization combinations against hepatitis C virus (HCV). Six groups of mice were immunized at weeks 0, 4, and 8 with different combinations of a ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2005.18.637
更新日期:2005-01-01 00:00:00
abstract::Cytomegalovirus (CMV) is a significant cause of morbidity and mortality in immunocompromised hosts, many of whom undergo significant periods of lymphopenia. However, the impact of lymphopenia and subsequent immune reconstitution on T cell responses and pulmonary pathology are poorly understood. Using a model of primar...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2015.0005
更新日期:2015-06-01 00:00:00
abstract::Influenza virus infections remain a significant health burden worldwide, despite available vaccines. Factors that contribute to this include a lack of broad coverage by current vaccines and continual emergence of novel virus strains. Further complicating matters, when influenza viruses infect a host, severe infections...
journal_title:Viral immunology
pub_type: 杂志文章,评审
doi:10.1089/vim.2017.0138
更新日期:2018-03-01 00:00:00
abstract::Avian infectious bronchitis virus (IBV), which is prevalent in many countries causing severe economic loss to the poultry industry, causes infectious bronchitis (IB) in birds. Recombinant single-chain variable fragments (scFvs) have been proven to effectively inhibit many viruses, both in vitro and in vivo, and they c...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2014.0104
更新日期:2015-09-01 00:00:00
abstract::The involvement of retroviruses in the etiopathogenesis of autoimmunity is discussed. Recently antibodies against p24 of HIV-1 were described in patients from Texas with SLE. Therefore we investigated the presence of these antibodies in our SLE collective (German caucasians and blacks from Michigan) employing ELISA an...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1992.5.229
更新日期:1992-10-01 00:00:00
abstract::Adenoviral vector has been extensively studied as a vaccine platform because of its ability to induce potent cellular and humoral immunity. One main advantage of adenoviral vectors is their natural tropism for mucosal surfaces, which makes them ideal for the purpose of mucosal vaccination against pathogens that prefer...
journal_title:Viral immunology
pub_type: 杂志文章,评审
doi:10.1089/vim.2005.18.283
更新日期:2005-01-01 00:00:00
abstract::Synthetic oligodeoxynucleotides (ODNs) such as CpG can stimulate B and plasmacytoid dendritic cells in vertebrate immune systems. Several studies showed that non-CpG ODNs could also induce strong stimulation of B and T cells. PyNTTTTGT ODNs, non-CpG ODNs, can activate and cause immunoglobulin secretion by B cells and ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2008.0073
更新日期:2009-04-01 00:00:00
abstract::Besides being an important target in the antiretroviral therapy against the human immunodeficiency virus type 1 (HIV-1), the HIV-1 reverse transcriptase (RT) enzyme has potential as a vaccine antigen. In this study, we explored the ability of plasmid-encoded RT to induce cell-mediated immune responses. The strategy fo...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2012.0083
更新日期:2013-04-01 00:00:00
abstract::Insights into the host factors that contribute to an effective antiviral immune response may be obtained by examining global gene expression in simian human immunodeficiency virus (SHIV)-infected nonhuman primates that exhibit different virological outcomes. Immune responses and gene expression profiles in peripheral ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2008.0076
更新日期:2008-12-01 00:00:00
abstract::The Primo-SHM trial, a multicenter randomized trial comparing no treatment with 24 or 60 weeks of combination antiretroviral therapy (cART) during primary human immunodeficiency virus (HIV) infection (PHI), recently demonstrated that temporary early cART lowered the viral set point and deferred the need for re-initiat...
journal_title:Viral immunology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1089/vim.2014.0094
更新日期:2015-04-01 00:00:00
abstract::Serum antibodies against the E6 and E7 proteins of human papillomavirus (HPV) 16 and 18 are associated with cervical cancer. The aim of this study was to investigate the presence of local antibodies against HPV in cervicovaginal washings (CWs). In this study antibodies against the native HPV16 and HPV18 E6/E7 proteins...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/08828240152716655
更新日期:2001-01-01 00:00:00
abstract::Subjects > or = 55 years of age were immunized with attenuated varicella zoster virus (VZV) vaccine (live) or with the same vaccine, which had been heated to 56 degrees C for 7 days (killed). The ability of subjects' blood lymphocytes to lyse target cells infected with VZV was determined before and 3 months after immu...
journal_title:Viral immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1089/vim.1996.9.241
更新日期:1996-01-01 00:00:00
abstract::BALB/c mice, preimmunized with a protective dose of native herpes simplex virus type 1 glycoprotein D (ngD1) vaccine, were depleted of selected immunocyte populations in vivo using monoclonal antibodies directed at Thy1+, L3T4+, or Lyt2+ cells. Following immunization and depletion, animals were inoculated with varied ...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1992.5.151
更新日期:1992-07-01 00:00:00
abstract::This study aimed to investigate the seroprevalence of cytomegalovirus (CMV) infection and risk factors associated with CMV acquisition among pregnant women in Zimbabwe. In a cross-sectional study, pregnant women were recruited in late gestation, seeking antenatal care at council clinics in three high-density suburbs i...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2019.0024
更新日期:2019-09-01 00:00:00
abstract::Bacterial flagellin is a potent adjuvant that enhances adaptive immune responses to a variety of protein antigens. The vaccinia virus antigens L1R and B5R are highly immunogenic in the context of the parent virus, but recombinant forms of the proteins are only weakly immunogenic. Therefore we evaluated the humoral res...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2009.0107
更新日期:2010-04-01 00:00:00
abstract::To investigate the capability of different routes of immunization with replication-competent recombinant adenovirus to induce antigen-specific antibody responses, we immunized cotton rats with a human adenovirus type 5 (HAd5) vector expressing the glycoprotein D (gD) of bovine herpesvirus type 1 (BHV-1) (gD-dE3). Diff...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1998.11.79
更新日期:1998-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an economically important infectious disease of swine. Mechanisms governing activation of the innate immune response to PRRSV remain to be elucidated. Virulence differences observed between PRRSV isolates have been attributed to replication ab...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2008.0038
更新日期:2008-09-01 00:00:00
abstract::Most strategies for reducing global measles morbidity and mortality and eliminating measles are based on the ability to enhance immune responses to measles virus. Challenges to measles elimination and eradication are based in part on the need to sustain high levels of population immunity to interrupt transmission of m...
journal_title:Viral immunology
pub_type: 杂志文章,评审
doi:10.1089/08828240152716556
更新日期:2001-01-01 00:00:00
abstract::Stage of liver fibrosis is critical for treatment decision and prediction of outcomes in chronic hepatitis C (CHC) patients. We evaluated the diagnostic accuracy of transient elastography (TE)-FibroScan and noninvasive serum markers tests in the assessment of liver fibrosis in CHC patients, in reference to liver biops...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2017.0134
更新日期:2018-05-01 00:00:00
abstract::California (CAL) serogroup Bunyaviruses are significant agents of arboviral encephalitis in humans. They are maintained and transmitted in nature by mosquitoes to preferred vertebrate amplifying hosts. The G2 envelope glycoprotein of La Crosse virus (LAC) was proposed by Ludwig et al. to be a determinant for virus att...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2000.13.201
更新日期:2000-01-01 00:00:00
abstract::Reovirus has previously been classified into three serotypes based on hemagglutination inhibition assays. In the present study, the specificity of cytotoxic T lymphocytes (CTL) generated in reovirus type 3-infected C3H/HeN mice was investigated at the population and clonal levels. Short-term CTL lines generated in res...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1991.4.167
更新日期:1991-10-01 00:00:00
abstract::Th17 cells and the secreting cytokines play an important role in the immune response and inflammation that is induced by hepatitis B virus (HBV). However, it remains not fully elucidated how the antiviral agents affect Th17 cytokines and signal pathway. Telbivudine therapy has been proved to inhibit HBV replication ef...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.2013.0032
更新日期:2013-10-01 00:00:00
abstract::C57BL/6 mice can generate a type-specific and class IH-2Kb-restricted CTL response against histocompatible AKR/Gross murine leukemia virus (MuLV) cell surface antigen positive (GCSA+) tumor cells. These anti-AKR/Gross MuLV CTL are also known to lyse SC.Kb/623 target cells expressing the molecular MuLV clone AKR623 (de...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1994.7.51
更新日期:1994-01-01 00:00:00
abstract::In cattle, cells with functional characteristics similar to those of natural killer (NK) cells are difficult to detect. However, lymphokine-activated killer (LAK) cells can be detected readily after in vitro activation of peripheral blood mononuclear leukocytes (PBML) with interleukin-2 (IL-2). In the present study, w...
journal_title:Viral immunology
pub_type: 杂志文章
doi:10.1089/vim.1991.4.259
更新日期:1991-01-01 00:00:00