Abstract:
:The objective of our study was to investigate chondrogenesis potential of human adipose-derived mesenchymal stromal cells (MSCs), using as a positive control a human source of cartilage-derived progenitor cells (PCs). This source of PCs was recently described by our group and dwells on the surface of nasoseptal cartilage. Histological analysis using Safranin O staining and immunofluorescence for actin filaments and collagen type II was performed on three-dimensional (3D) pellet cultures. Cartilage PCs and adipose MSCs showed similarities in monolayer culture related to cell morphology and proliferation. Our 3D pellet cultures substantially reduced the actin stress and after 21 days under chondrogenic medium, we observed an increase in the pellet diameter for cartilage PCs (7.4%) and adipose MSCs (21.2%). Adipose-derived MSCs responded to chondrogenic stimulus, as seen by positive areas for collagen type II, but they were not able to recreate a mature extracellular matrix. Using semi-quantitative analysis, we observed a majority of Safranin O areas rising from blue (no stain) to orange (moderate staining) and no changes in fibroblastic morphology (P < 0.0001). For cartilage PCs, chondrogenic induction is responsible for morphological changes and a high percentage of matrix area/number of cells (P ≤ 0.0001), evaluated by computerized histomorphometry. Morphological analyses reveal that adipose-derived MSCs were not able to recreate a bioengineered cartilage. The cost of culture was reduced, as the cartilage PCs under growth-factor free medium exhibit a high score for cartilage formation compared with the induced adipose mesenchymal stromal cells (P = 0.0021). Using a pellet 3D culture, our cartilage PCs were able to produce a cartilage tissue in vitro, leading to the future development of bioengineered products.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Baptista LS,Silva KR,Pedrosa CS,Amaral RJ,Belizário JV,Borojevic R,Granjeiro JMdoi
10.1111/aor.12121subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1068-75issue
12eissn
0160-564Xissn
1525-1594journal_volume
37pub_type
杂志文章abstract::A pneumatically driven artificial heart with a tubular silicone rubber membrane and disc valves was used for functional heart replacement in the paracorporeal mode. A fluidic drive system allows adjustment of the heart rate, positive and negative pressures and systole/diastole ratio. Since August, 1977, the artificial...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1978.tb03465.x
更新日期:1978-08-01 00:00:00
abstract::Bovine pericardium is a widely utilized biomaterial. Usually, after harvesting, it is advantageous that the pericardium be immersed in glycerol to improve its shelf life. This can induce some degree of toxicity in the material. The studies were performed in compliance with the rules of ISO 10993 and OECD 487, in the b...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00542.x
更新日期:2008-04-01 00:00:00
abstract::A totally implantable centrifugal artificial heart has been developed in which a pivot bearing supported centrifugal pump is used as a blood pump. The following have been adopted as blood contacting materials in our pump: titanium alloy (Ti-6A1-4V) for the housing and impeller, alumina ceramic (Al2O3) for the male piv...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.6184r.x
更新日期:1998-09-01 00:00:00
abstract::Although it is now understood that trained skeletal muscle can generate enough steady-state power to provide significant circulatory support, there are currently no means by which to tap this endogenous energy source to aid the failing heart. To that end, an implantable muscle energy converter (MEC) has been construct...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1111/j.1525-1594.2005.29108.x
更新日期:2005-09-01 00:00:00
abstract::The Genius is a convenient single-pass batch hemodialysis system. Dialysate is heated during the preparation phase. Once the container is filled at one of the three available starting temperatures, dialysate cools spontaneously. As dialysate temperature plays an important role in hemodynamic stability, knowledge of th...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00981.x
更新日期:2010-12-01 00:00:00
abstract::Over the last 5 years, 12 patients received ventricular assist devices (VADs) while in postcardiotomy cardiogenic shock. There were 7 male and 5 female patients ranging in age from 22 to 73 years (average age, 54). Eight patients underwent surgery for valve replacements, 3 for coronary artery bypass graftings, and 1 f...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1993.tb00608.x
更新日期:1993-07-01 00:00:00
abstract::In this study two aspects of hybrid functional electrical stimulation (FES) orthoses were investigated: joint motion constraints and FES control strategies. First, the effects of joint motion constraints on the gait of normal subjects were investigated using modern motion analysis systems, including electromyogram (EM...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章
doi:10.1111/j.1525-1594.1997.tb04648.x
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND:The use of ventricular assist devices (VAD) as a bridge to heart transplant (HT) is increasing, while HT volume remains stagnant. This may portend longer waiting times and an otherwise more competitive environment for all patients on the HT waiting list. METHODS:A retrospective analysis of patients who were...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13833
更新日期:2020-10-01 00:00:00
abstract::Pyridoxalated-hemoglobin-polyoxyethylene conjugate (PHP), which is made from out-dated human red blood cells by two major chemical modifications, namely pyridoxalation and conjugation with polyoxyethylene (POE), is currently under development as a physiological oxygen carrier. This study assessed the effects of PHP-88...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1993.tb00424.x
更新日期:1993-03-01 00:00:00
abstract::Present-day computational fluid dynamics (CFD) techniques can be used to analyze the behavior of fluid flow in a variety of pumps. CFD can be a powerful tool during the design stage for rapid virtual prototyping of different designs, analyzing performance parameters, and making design improvements. Computational flow ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::This study probed the changes of keratocytes cultured under simulated microgravity. Keratocytes were isolated from rabbit corneas using collagenase digestion method. Cells were seeded in a 55-mL capacity high-aspect-ratio vessel (HARV) of rotary cell culture system (RCCS) at a density of 1 x 10(4) cells/mL. Dehydrated...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2007.00440.x
更新日期:2007-09-01 00:00:00
abstract::A new intravascular pumping oxygenator (IVPO) was developed for intravascular gas exchange and circulatory assistance in critically ill patients with respiratory and circulatory failure. The IVPO utilizes new silicone hollow fibers (diameter, 1 mm; membrane width, 50 microns) and consists of two driving tubes for the ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00703.x
更新日期:1997-01-01 00:00:00
abstract::Percutaneous femoral venoarterial (VA) or jugular venovenous (VV) extracorporeal membrane oxygenation (ECMO) can result in delivery of hypoxic blood to the brain, coronaries, and upper extremities. Additionally, VA-ECMO by percutaneous femoral artery cannulation may compromise perfusion to the lower limbs. Use of near...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2012.01496.x
更新日期:2012-08-01 00:00:00
abstract::Aseptic joint loosening is a key factor that reduces the life span of arthroplasty. There are currently few effective treatments for joint loosening except surgical revision. We explored the inhibitory effects of tumor necrosis factor (TNF)-alpha-targeted small interfering RNA (siRNA) on particle-induced inflammatory ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2010.01175.x
更新日期:2011-07-01 00:00:00
abstract::To evaluate their toxicity at the cellular level, middle molecules from uremic serum were incubated with erythrocytes from healthy subjects and the activity of the enzyme Delta-aminolevulinic acid dehydrase (D-ALA-D) and peroxidative hemolysis were investigated. Uremic middle molecules caused a significant decrease of...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1978.tb01626.x
更新日期:1978-11-01 00:00:00
abstract::The aim of this study was to evaluate the safety and performance of the Arrow CorAide left ventricular assist system (LVAS) (Arrow International, Reading, PA, USA), a continuous-flow left ventricular assist device, as bridge to transplantation or recovery as well as destination therapy in patients with New York Heart ...
journal_title:Artificial organs
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1525-1594.2012.01539.x
更新日期:2013-02-01 00:00:00
abstract::The use of recombinant human erythropoietin (rHuEpo) has revolutionized anemia management of early and late stages of chronic kidney disease. Darbopoietin is also now available for the treatment of anemia of chronic kidney disease. In addition, rHuEpo has been used for the treatment of anemia observed in critical illn...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1046/j.1525-1594.2003.07287.x
更新日期:2003-09-01 00:00:00
abstract::Microcapsules containing insulin crystals or islets of Langerhans were made by extruding a mixture of insulin crystals or islets and sodium alginate into a calcium chloride solution, and then coating it with poly-l-lysine. When these microcapsules were incubated at 37 degrees C, insulin could be detected readily in th...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1983.tb04187.x
更新日期:1983-05-01 00:00:00
abstract::Hypertension is an important primary etiology of end-stage renal disease as well as a major factor responsible for progression of renal disease due to other causes. Multiple genetic and environmental factors are responsible for the variable prevalence of hypertension in various parts of the world. Although the frequen...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2002.07067.x
更新日期:2002-09-01 00:00:00
abstract::The goal of this study was to determine whether porcine islets encapsulated in hollow fibers made of AN69 copolymer can correct hyperglycemia in diabetic mice and provide normal tolerance to a glucose challenge. In vitro perifusion of hollow fibers demonstrated the rapid kinetics of insulin release in response to gluc...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.05087.x
更新日期:1998-04-01 00:00:00
abstract::This article presents computational studies on the effects of inlet guide vanes (IGVs) on the flow pattern and shear stress in a centrifugal blood pump. The effect of IGVs is to introduce a pre-swirl to fluid particles entering the impeller with the intention that the fluid particles will travel along the blade profil...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2002.06917.x
更新日期:2002-06-01 00:00:00
abstract::An increasing amount of evidence points to cerebral embolization during cardiopulmonary bypass (CPB) as the principal etiologic factor of neurologic complications. In this study, the capability of capturing and classification of gaseous emboli and pressure drop of three different membrane oxygenators (Sorin Apex, Teru...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00955.x
更新日期:2009-11-01 00:00:00
abstract::Bacillus Calmette-Guerin (BCG) has been shown to have therapeutic effects on asthma through CD4+CD25+ regulatory T cells (Tregs). We sought to assess pretreatment with inactivated BCG on CD4+CD25+ Tregs and its functional and structural effects in rat asthma model. The rat asthma model was established using ovalbumin ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12566
更新日期:2016-04-01 00:00:00
abstract::To expand the usage of the centrifugal pump (CP) in open heart surgery, we performed two studies. In the first, we evaluated pulsatile flow in the CP. In vitro pump performance of the Terumo Capiox pump (TCP) and the Sarns Delphin pump (SDP) and increase of free hemoglobin (mg/dl) after driving 6 h were investigated u...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1994.tb03393.x
更新日期:1994-09-01 00:00:00
abstract::Although several left ventricular assist devices (LVADs) have been used widely, remote monitoring of LVAD parameters has been available only recently. We present our remote monitoring experience with an axial-flow LVAD (HeartAssist-5, MicroMed Cardiovascular, Inc., Houston, TX, USA). Five consecutive patients who were...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12144
更新日期:2013-09-01 00:00:00
abstract::Our study investigated the reliability of appearance of rapid atrial swirl flow (RASF) by ultrasonography (US) in the right atrium (RA), which occurred as a result of rapid isotonic saline infusion (RISI) into the central venous catheter (CVC), in predicting catheter tip position. This prospective observational study ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13281
更新日期:2018-12-01 00:00:00
abstract::To assess the influence of hydration state on plasma volume (PV) changes during ultrafiltration, 11 clinically normhydrated patients on maintenance hemodialysis were studied intraindividually during 2 hydration states differing by 2-15% of lean body mass (LBM). Plasma volume was measured continually during a 15-min ul...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1995.tb02352.x
更新日期:1995-05-01 00:00:00
abstract::Only a small number of English hospitals provide postcardiotomy venoarterial extracorporeal membrane oxygenation (VA-ECMO) and there are doubts about its efficacy and safety. The aim of this service evaluation was to determine local survival rates and report on patient demographics. This was a retrospective service ev...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13647
更新日期:2020-07-01 00:00:00
abstract::Current hemodialysis treatment is insufficient because of intermittent treatment and loss of tubular function. In order to overcome the loss of tubular function, a bioartificial kidney has been developed consisting of continuous hemofiltration (CHF) with 10 L/day of filtrate and a bioartificial tubule device using pro...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章
doi:10.1111/j.1525-1594.2004.07323.x
更新日期:2004-01-01 00:00:00
abstract::In patients undergoing exchange-transfusion with hemoglobin (Hb)-based oxygen (O₂) carriers (HBOC), native Hb coexists with newly transfused Hb. The two Hb types share the same arterial and venous PO₂, but their affinities for O₂ vary. A simple spreadsheet model is described aiming at evaluating the contribution of ea...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2011.01296.x
更新日期:2012-02-01 00:00:00