Abstract：
BACKGROUND:K-ras oncogene mutations have been identified in up to 95% of pancreatic cancers, implying their critical role in their molecular pathogenesis. However, the earliest stage in which K-ras mutations can be detected in potential precursor lesions of pancreatic cancer remains unclear. This study evaluates pancreatic ductal hyperplasia in the setting of chronic pancreatitis, which predisposes to pancreatic cancer development, for K-ras codon 12 and 13 mutations. METHODS:Paraffin-embedded surgical specimens from 42 patients with chronic pancreatitis were examined microscopically for the presence of ductal hyperplasia. Both hyperplastic and nonhyperplastic ducts were microdissected from the specimens that contained hyperplasia (11 of 42). Four of the remaining specimens without hyperplasia served as controls. Genomic DNA was extracted, and polymerase chain reaction and amplification of the K-ras oncogene was performed. Polymerase chain reaction products were evaluated by means of hybridization to mutant specific oligonucleotide probes and by means of automated DNA sequencing. RESULTS:K-ras codon 12 mutations representing glycine to valine substitutions were present in 2 of (18%) 11 patients with ductal hyperplasia. No mutations were found in the controls without ductal hyperplasia. CONCLUSIONS:Our study supports the premise that K-ras mutations develop in a subset of chronic pancreatitis associated hyperplasia and provides a genetic basis for the potential progression of chronic pancreatitis to pancreatic cancer.
journal_name
Surgeryjournal_title
Surgeryauthors
Rivera JA,Rall CJ,Graeme-Cook F,Fernández-del Castillo C,Shu P,Lakey N,Tepper R,Rattner DW,Warshaw AL,Rustgi AKdoi
10.1016/s0039-6060(97)90181-1subject
Has Abstractpub_date
1997-01-01 00:00:00pages
42-9issue
1eissn
0039-6060issn
1532-7361pii
S0039-6060(97)90181-1journal_volume
121pub_type
杂志文章相关文献
SURGERY文献大全abstract：BACKGROUND:The role of routine central lymph node dissection (CLND) for papillary thyroid cancer (PTC) remains controversial. The aim of this study was to evaluate the impact of routine CLND after total thyroidectomy (TTx) in the management of patients with PTC who were clinically node negative at presentation with emp...
journal_title：Surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/j.surg.2011.09.003
更新日期：2011-12-01 00:00:00
abstract：:Thirty-ones limbs of 29 consecutive patients undergoing bypass grafts to the tibial or peroneal arteries were studied. Twenty-five limbs had ischemic rest pain with or without localized gangrene, and six had incapacitating claudication. The mean segmental pressure indices were upper thigh, 1.16 +/- 0.36; above knee, 0...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：BACKGROUND:The surgical management and follow-up strategy in patients with medullary thyroid carcinoma (MTC) remain controversial because of the lack of data on the natural history of these tumors and their patterns of progression. METHODS:We reviewed the records of all patients who underwent a cervical operation for ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/s0039-6060(03)00408-2
更新日期：2003-12-01 00:00:00
abstract：:The effectiveness of prosthetic femoropopliteal bypass grafts to salvage limbs and maintain long-term patency in lower extremities with a limited outflow tract is controversial. To evaluate the results of prosthetic grafts and compare them with the results of vein bypass grafts in limbs with a limited outflow tract, w...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:Lipopolysaccharide increases intestinal mucosal permeability to hydrophilic compounds such as chromium 51-labeled edetate (51Cr-EDTA). We sought to determine whether this phenomenon is partly mediated by lipopolysaccharide-induced mesenteric hypoperfusion. We assessed permeability in an isolated segment of ileum by me...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：BACKGROUND:The benefit of removing the splenic lymph nodes in patients with proximal gastric cancer has been controversial. The purpose of our study was to investigate the importance of performing a splenic hilar lymph node dissection without splenectomy in patients undergoing total gastrectomy for gastric cancer. MET...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2020.07.053
更新日期：2021-02-01 00:00:00
abstract：BACKGROUND:An understanding of the regeneration power and operative risk of steatotic livers after hepatectomy is still unclear. We evaluated the volume regeneration and outcome of steatotic livers after donor hepatectomy. METHODS:Fifty-four, consecutive living liver donors from September 2002 to December 2003 were ev...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2005.09.007
更新日期：2006-04-01 00:00:00
abstract：BACKGROUND:The hypermetabolic response to severe burn is characterized by muscle protein catabolism. Current opinion states that the hypermetabolic state resolves soon after complete wound closure. Clinically, we have witnessed that burned children appear to be hypermetabolic and catabolic long after full healing of th...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2000.108059
更新日期：2000-08-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to determine whether intraoperative parathyroid hormone (IOPTH) assay improved results of reoperations. METHODS:One hundred two patients with persistent/recurrent sporadic primary hyperparathyroidism underwent 108 reoperations (1996-2002). IOPTH was not used (n=58) from 1996-19...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2003.08.007
更新日期：2003-12-01 00:00:00
abstract：BACKGROUND:Biologic mesh choice in ventral hernia repair is challenging due to lack of prospective data. This study examines long-term, single-center biologic mesh outcomes. METHODS:Prospective operative outcomes data was queried for open ventral hernia repair with biologic mesh. Univariate and multivariate analysis w...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2016.07.008
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Despite successful revascularization of ischemic extremities, multiorgan dysfunction syndrome develops in some patients. Mechanisms responsible for this are not known; however, the gastrointestinal tract has been implicated as a possible mediator. Our objective was to demonstrate increased intestinal mucosal...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/s0039-6060(96)80076-6
更新日期：1996-09-01 00:00:00
abstract：:We estimated ambulatory venous pressure (AVP) noninvasively with a new technique of quantitative photoplethysmography (PPG). Postural changes of hydrostatic pressure permitted in vivo calibration of the PPG. We recorded quantitative PPG and AVP in 14 normal subjects and 14 patients with postthrombotic chronic venous i...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：BACKGROUND:Prolonged warm ischemic injury in non-heart-beating donors (NHBDs) significantly affects hepatic allograft function after liver transplantation (LTx). METHODS:The effects of FK506 and the platelet activating factor antagonist E5880 on postoperative function of hepatic allografts procured from NHBDs were eva...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：BACKGROUND:Previous studies using simulation-based curricula have focused largely on technical skills. We developed a set of simulation-based modules that focus on intraoperative decision making. The objective of this study was to conduct a faculty evaluation of: (1) the usefulness of 4 newly developed, simulation-base...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2010.10.010
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:There are few population-based studies addressing the survival after resection for esophageal cancer. This study represents an update of a nationwide Swedish cohort initiated in 1987. METHODS:Based on data from the Swedish Patient Register, Swedish Cancer Register, and histopathologic records, 1,008 patient...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2012.03.025
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:Ventral incisional hernia is the most common long-term complication after an abdominal operation. Among newly diagnosed colorectal cancer patients, we screened the preoperative plasma proteome to explore predictive markers for the development of an incisional hernia. METHODS:We utilized preoperative plasma ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2016.08.025
更新日期：2017-03-01 00:00:00
abstract：:This study examined whether treatment with the oxygen free radical scavengers, superoxide dismutase and catalase, could improve functional recovery in hearts subjected to regional ischemia and global cardioplegic arrest. Regional left ventricular (LV) function was assessed in open chest pigs with sonomicrometry and mi...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：BACKGROUND:Understanding predictors of mortality in bariatric surgery enables surgeons to use these factors for analysis of risk-adjusted mortality and aids in the surgical decision making and informed consent process. OBJECTIVES:To evaluate the effect of patient characteristics (age, gender, race, and payer type), pr...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2011.05.020
更新日期：2011-08-01 00:00:00
abstract：BACKGROUND:Patients undergoing ventral hernia repair (VHR) are at risk of an inadvertent enterotomy during surgery. Inadvertent enterotomies potentially contaminate the surgical field presenting a management dilemma for the surgeon. The aim of our study was to define the incidence and risk factors for a recognized inad...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.04.003
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to determine if systemic acidosis induced by peritoneal absorption of carbon dioxide (CO2 ) during laparoscopy plays a role in CO2 pneumoperitoneum-mediated attenuation of the acute phase inflammatory response associated with perioperative sepsis. The influence of hepatic polymo...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2005.01.005
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:We carried out a prospective, randomized, 4-arm study including control arm, blinding of examiners to determine effectiveness of computer-based video instruction (CBVI) and different types of expert feedback (concurrent and summary) on learning of a basic technical skill. METHODS:Using bench models, partici...
journal_title：Surgery
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.surg.2006.09.012
更新日期：2007-04-01 00:00:00
abstract：BACKGROUND:Recent emphasis in medical education has been to encourage students to pursue primary care careers. This could have a negative impact on applications to surgical residencies. METHODS:To determine what factors are most influential for a student to pursue a surgical career in spite of this environment, third-...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2000.108214
更新日期：2000-08-01 00:00:00
abstract：:Formation of biliary calculi caused by hemobilia is rare. Including the two cases reported here, there are only a total of four in the literature. The characteristics of these calculi in vitro, on computerized tomographic scan, and cholecystography are described. The condition for the occurrence seems to be that blood...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：BACKGROUND:Adhesion of polymorphonuclear leukocytes (PMNs) to endothelial cells is mediated partially by CD11/CD18 integrins. The purpose of this study was to define (1) the response of PMNs to anti-CD18 monoclonal antibody binding, and (2) the mechanism responsible for anti-CD18 monoclonal antibody-mediated decreases ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：BACKGROUND:Surgical enteral access prior to multimodality treatment for esophageal cancer is controversial as dysphagia is often used for feeding tube referral. We hypothesized that enteral access before neoadjuvant chemoradiation for esophageal cancer provides no benefit compared to that placed during definitive esoph...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2017.09.046
更新日期：2018-04-01 00:00:00
abstract：:Metabolic alkalosis secondary to chloride depletion, especially following gastrointestinal surgery and associated with acute renal failure, is a frequent clinical occurrence. Management of the resultant acid-base disturbance mandates chloride replacement. The presence of oliguria limits the choice of accompanying cati...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:Restoration of circulation following occlusion of an aortofemoral graft was attempted in 50 limbs in 38 patients. Progressive arteriosclerosis in the femoral vessels was the cause of graft limb thrombosis in most instances. Profundaplasty was employed in 47 limbs and femoral-popliteal bypass in two to provide effectiv...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：BACKGROUND:Intraoperative parathyroid hormone (IOPTH) monitoring in parathyroidectomy for multigland disease is less clear than for single-gland disease. This study assesses the role of IOPTH for hyperplasia. METHODS:A prospective database revealed 45 patients with hyperplasia undergoing parathyroidectomy utilizing IO...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2004.05.060
更新日期：2004-12-01 00:00:00
abstract：:This investigation documented the effect of protamine sulfate pretreatment on the adverse hemodynamic and hematologic sequela of rapid intravenous protamine administration in heparinized dogs having undergone prior implantation of aortic prostheses. Fourteen dogs underwent infrarenal aortic replacement with 6 mm inner...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：BACKGROUND:The effect of trehalose on the preservation of canine lungs was studied with the use of Euro-Collins solution (ECS). METHODS:In group 1, five lungs were perfused and preserved with a modified ECS in which trehalose (35.0 gm/L) was used instead of glucose. In group 2, six lungs were perfused and preserved wi...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
