Expression of epidermal growth factor and transforming growth factor alpha during ulcer healing. Time sequence study.

Abstract:

BACKGROUND:Growth factors such as epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha) have been shown to share common receptor (EGFR) and to accelerate ulcer healing due to stimulation of cell proliferation, but the time sequence of expression of EGF and TGF alpha during ulcer healing has not been investigated. In this study the rate of cell proliferation and the gastric secretion and gene expression of mRNA for EGF and TGF alpha were determined during ulcer healing. METHODS:Gastric ulcers were induced in 150 Wistar rats by serosal application of 100% acetic acid (ulcer area, 14 mm2). Some of these animals were also equipped with a gastric fistula for the assessment of gastric secretion during ulcer healing. The animals were killed 0, 2, 4, 6, or 8 days after ulcer induction, and the ulcer area was determined. The mucosal sections with gastric ulcer were immunostained for proliferating cell nuclear antigen (PCNA) and for immunoexpression of EGF, TGF alpha, and EGFR. The expression of mRNA EGF and mRNA TGF alpha was also determined in the ulcer margin by reverse transcriptase (RT) polymerase chain reaction (PCR) using specific primers. RESULTS:Two, 4, 6, and 8 days after ulcer induction the gastric ulcer area was gradually reduced from the initial size (day 0) by 47%, 70%, 80%, and 87%, respectively, and this was accompanied by an increase in PCNA with its maximum on day 4. The gastric acid and pepsin secretion was significantly reduced by 75% and 79%, respectively, on day 2 after ulcer induction but then the secretion tended to return to normal value by day 8. The expression of EGF, TGF alpha, and EGFR was negligible on day 0 but increased significantly during the healing, reaching maximum on day 4. Expression of EGF mRNA was detected on days 2, 4, and 6, and that of TGF alpha mRNA on days 2, 4, 6, and 8 after ulcer induction, with the most intense signals for both transcripts observed on day 2. CONCLUSIONS:1) The enhancement in cell proliferation during ulcer healing may be mediated by increased release of EGF and TGF alpha; 2) the expression of EGF and TGF alpha mRNA precedes the overexpression of these growth factors at the ulcer margin during ulcer healing; and 3) the overexpression of growth factors coincides with the inhibition of gastric secretion and increased blood flow at the ulcer margin, indicating that these factors affect gastric secretion and blood flow in the course of ulcer healing.

journal_name

Scand J Gastroenterol

authors

Konturek PC,Brzozowski T,Konturek SJ,Ernst H,Drozdowicz D,Pajdo R,Hahn EG

doi

10.3109/00365529709025056

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

6-15

issue

1

eissn

0036-5521

issn

1502-7708

journal_volume

32

pub_type

杂志文章
  • Immune regulation in chronic hepatitis C virus infection.

    abstract::The immunological result of infection with Hepatitis C virus (HCV) depends on the delicate balance between a vigorous immune response that may clear the infection, but with a risk of unspecific inflammation and, or a less inflammatory response that leads to chronic infection. In general, exhaustion and impairment of c...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3109/00365521.2016.1170875

    authors: Hartling HJ,Ballegaard VC,Nielsen NS,Gaardbo JC,Nielsen SD

    更新日期:2016-11-01 00:00:00

  • Helicobacter pylori-associated gastritis and dyspepsia. The influence on migrating motor complexes.

    abstract::Twenty-five patients with dyspepsia were included. In 19 patients with a median age of 48 (range, 20-72) years endoscopy and histologic examination of biopsy specimens from the antrum and corpus of the stomach showed Helicobacter pylori-positive gastritis as the only pathologic finding. In six patients with a median a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529409090451

    authors: Qvist N,Rasmussen L,Axelsson CK

    更新日期:1994-02-01 00:00:00

  • Fenfluramine and idiopathic pain: a serotonergic study in non-psychiatric patients with functional gastrointestinal disorder.

    abstract:BACKGROUND:We investigated prolactin and cortisol response to fenfluramine in non-psychiatric patients with chronic functional gastrointestinal disorder (FGD). METHODS:Sixty milligrams fenfluramine was given orally to 55 subjects without any DSM-III axis-1 psychopathology and 29 healthy control subjects matched for se...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.1080/00365529850171602

    authors: Tanum L,Bråtveit-Johansen K,Malt UF

    更新日期:1998-07-01 00:00:00

  • Gastrin secretion before and after gastric bypass surgery for morbid obesity.

    abstract::Fasting, meal-stimulated, and insulin-hypoglycemia-stimulated serum gastrin levels were measured before and 3 and 12 months after gastric bypass surgery in nine obese patients. The basal gastrin concentration was unaffected by the operation. After a meal both serum gastrin and blood glucose levels were significantly i...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528109180995

    authors: Schrumpf E,Giercksky KE,Nygaard K,Fausa O

    更新日期:1981-01-01 00:00:00

  • Regional distribution and localization of zinc and metallothionein in the intestine of rats fed diets differing in zinc content.

    abstract:BACKGROUND:Zinc (Zn) is protective and enhances epithelial repair in gut diseases. In this study we investigate the localization and distribution of Zn and its binding protein, metallothionein (MT), in the gut of rats fed diets varying in Zn content. METHODS:Male-Sprague Dawley rats were fed low, normal, high, or exce...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655299750025895

    authors: Tran CD,Butler RN,Howarth GS,Philcox JC,Rofe AM,Coyle P

    更新日期:1999-07-01 00:00:00

  • Quantitative electron microscopical studies of parietal cells after antral exclusion in the rat.

    abstract::Antral exclusion was performed in seven adult male Sprague-Dawley rats, which were killed 10 weeks later. Fasting serum gastrin concentrations were almost four times higher than in unoperated controls. There was a slight increase in the thickness of the gastric mucosa, but quantitative electron microscopy showed no si...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528209181059

    authors: Helander HF,Liedberg G,Rehfeld JF

    更新日期:1982-01-01 00:00:00

  • Fasting blood levels of gastrin, somatostatin, and pancreatic polypeptide in peptic ulcer disease.

    abstract::Fasting plasma levels of immunoreactive gastrin, somatostatin, and pancreatic polypeptide (PP) were determined in 67 patients with an endoscopically proven duodenal, pyloric, or prepyloric ulcer. Pretreatment gastrin (45.6 +/- 53.6 pmol/l, mean +/- S.D.) and somatostatin (54.5 +/- 27.5 pg/ml) did not differ significan...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365528209181048

    authors: Gustavsson S,Adami HO,Björklund O,Enander LK,Lundqvist G,Lööf L,Nordahl A

    更新日期:1982-01-01 00:00:00

  • The prerequisites for local lysolecithin formation in the human gallbladder. II. Studies on the positional specificity of the phospholipase A activity.

    abstract::The positional specificity of the phospholipase A in human gallbladder epithelium was studied by using biosynthetically radioabeled diacylphosphoglycerides as substrates. Diacylphosphoglyceride in 14C-palmitic acid-labeled, autoclaved E.coli was hydrolyzed under the formation of monoacylphosphoglyceride and fatty acid...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527809181915

    authors: Tagesson C,Norrby S,Sjödahl R

    更新日期:1978-01-01 00:00:00

  • Spontaneous anorectal pressure activity. Evidence of internal anal sphincter contractions in response to rectal pressure waves.

    abstract::To characterize spontaneous anorectal pressure activity and a possible relation between the activity in the rectum and the anal canal, 11 healthy female volunteers were investigated. Resting activities were obtained during 1-h recordings with a multi-channel perfused catheter measuring the pressure 1, 2, 3, 7, and 8 c...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528909092248

    authors: Sørensen SM,Gregersen H,Sørensen S,Djurhuus JC

    更新日期:1989-01-01 00:00:00

  • Randomized clinical trial of furazolidone for typhoid fever in children.

    abstract::The efficacy of furazolidone and chloramphenicol was compared in a randomized trial involving 133 children with bacteriologically confirmed typhoid fever. Sixty-five children were randomized to receive furazolidone, 7.5 mg/kg/day, and 68 children to receive chloramphenicol, 75 mg/kg/day. Both drugs were administered o...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365529309096065

    authors: Dutta P,Rasaily R,Saha MR,Mitra U,Manna B,Chakraborty S,Mukherjee A

    更新日期:1993-02-01 00:00:00

  • Erosive prepyloric changes in patients with end-stage renal failure undergoing maintenance dialysis treatment.

    abstract::We endoscoped 322 of 422 patients with end-stage renal failure undergoing maintenance dialysis treatment to determine the prevalence of erosive prepyloric changes (EPC) in uraemia. EPC grade 1 was found in 79 patients (25%), grade 2 in 16 (5%), and grade 3 in 43 (13%). EPC grades 2 and 3 were commoner among uraemic pa...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529008997602

    authors: Kang JY,Wee A,Choong HL,Wu AY

    更新日期:1990-07-01 00:00:00

  • Differential cell kinetics in the ileum and colon of germfree rats.

    abstract::Our aim was to study the cell kinetics and epithelial structure in the ileum and colon of conventional and germfree AGUS rats by means of a classic stathmokinetic technique. A slight hyperplasia was observed in the villi of germfree animals (p < 0.05), associated with a comparatively short cell cycle time. The crypt c...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529409096836

    authors: Alam M,Midtvedt T,Uribe A

    更新日期:1994-05-01 00:00:00

  • Pregnancy outcome in four women with inflammatory bowel disease treated with budesonide MMX.

    abstract:BACKGROUND:Maintaining disease remission throughout pregnancy in women with inflammatory bowel disease is of the utmost importance to decrease the risk of adverse outcome. In general, corticosteroids are safe to use during pregnancy, but no data exist in the specific use of budesonide MMX. We report four cases of budes...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2018.1533583

    authors: Vestergaard T,Jørgensen SMD,Christensen LA,Julsgaard M

    更新日期:2018-12-01 00:00:00

  • Inhibition of hepatic chylomicron remnant uptake in the cholestatic rat.

    abstract::[3H]retinal- or [3h]cholesterol- and [14C]fatty acid-labeled chylomicrons and chylomicron remnants were injected intravenously into normal rats and rats subjected to partial or total obstruction of the bile duct 8 or 48 h earlier. The clearance from plasma of the [3H]retinyl and the [3H]cholesteryl esters in 30-120 mi...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528009181798

    authors: Larsson B,Nilsson A

    更新日期:1980-01-01 00:00:00

  • Is transcatheter arterial embolization a safer alternative than surgery when endoscopic therapy fails in bleeding duodenal ulcer?

    abstract:OBJECTIVE:Emergency surgery after unsuccessful endoscopic therapy for bleeding duodenal ulcer has been reported to have a high mortality. Transcatheter arterial embolization (TAE) of the gastroduodenal artery is an alternative strategy when endoscopic therapy fails. This study is a retrospective analysis comparing thes...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365520903486109

    authors: Venclauskas L,Bratlie SO,Zachrisson K,Maleckas A,Pundzius J,Jönson C

    更新日期:2010-03-01 00:00:00

  • Total colectomy, mucosal proctectomy, and an ileal reservoir to an anal anastomosis. A comparison of short and long efferent legs.

    abstract::In a consecutive series of 46 possible candidates for total colectomy, mucosal proctectomy, and ileal reservoir to an anal anastomosis, we have compared the clinical outcome of group I, with a long efferent leg (12 cm), and group II, with a short efferent leg (3-5 cm). The surgical procedure was done in three steps: f...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528509088877

    authors: Hansen LK,Olsen PR,Simonsen L

    更新日期:1985-11-01 00:00:00

  • Significance of cell-surface expression of matrix metalloproteinases and their inhibitors on gastric epithelium and infiltrating mucosal lymphocytes in progression of Helicobacter pylori-associated gastritis.

    abstract:BACKGROUND:Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) have recently been shown to be important in tissue breakdown and remodeling in gut with inflammatory bowel disease. The role of MMPs and TIMPs remains largely unexplored in Helicobacter pylori-associated gastritis (HAG). The aim of this study was ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520410003245

    authors: Koyama S

    更新日期:2004-11-01 00:00:00

  • Evidence for predominant involvement of the B-cell system in the inflammatory process in Crohn's disease.

    abstract::The cellular infiltrate in the walls of resected ileum and colon specimens obtained from patients with Crohn's disease was analysed. Mononuclear cells in tissue sections were characterized by the use of surface membrane markers and immuno-histochemical and enzyme histochemical techniques. It was found that: [1] B cell...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527909179842

    authors: Meijer CJ,Bosman FT,Lindeman J

    更新日期:1979-01-01 00:00:00

  • Dipeptidyl peptidase IV (DP IV, CD26) in patients with inflammatory bowel disease.

    abstract:BACKGROUND:Dipeptidyl peptidase IV (DP IV, CD26), a serine protease with broad tissue distribution and known activity in serum, participates in T cell activation and promotes a Th1 cytokine response, a function in part attributable to its enzymatic activity. We hypothesized that the activity of DP IV in serum and expre...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655201750422675

    authors: Hildebrandt M,Rose M,Rüter J,Salama A,Mönnikes H,Klapp BF

    更新日期:2001-10-01 00:00:00

  • Usefulness of rectally administering [1-(13)C]-butyrate for breath test in patients with active and quiescent ulcerative colitis.

    abstract:OBJECTIVE:Impaired butyrate metabolism plays a part in ulcerative colitis (UC). To assess the usefulness of measuring butyrate metabolism as an indication of inflammatory activity, we investigated the rate of butyrate metabolism by breath test after administering [1-(13)C]-butyrate rectally to patients with UC. MATERI...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520600955070

    authors: Kato K,Ishii Y,Mizuno S,Sugitani M,Asai S,Kohno T,Takahashi K,Komuro S,Iwamoto M,Miyamoto S,Takayama T,Arakawa Y

    更新日期:2007-02-01 00:00:00

  • Symptom profiles in chronic peptic ulcer disease. A detailed study of abdominal and mental symptoms.

    abstract::Abdominal and mental symptoms were assessed in 103 outpatients with chronic peptic ulcer disease. Patients with present symptoms and a history of duodenal or prepyloric ulcer were included if they had no other disorder requiring treatment. A normal female population was used for comparison of mental symptoms. Besides ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3109/00365528509089674

    authors: Sjödin I,Svedlund J,Dotevall G,Gillberg R

    更新日期:1985-05-01 00:00:00

  • Modulation of arachidonic acid metabolism by olsalazine and other aminosalicylates in leukocytes.

    abstract::We investigated the action of the new aminosalicylate olsalazine (disodium azodisalicylate) on arachidonic acid metabolism in comparison with 5-aminosalicylic acid (5-ASA) and sulphasalazine (SASP) by in vitro incubation of cellular homogenates from human polymorphonuclear (PMNL) and mononuclear (MNL) leukocytes with ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529109037024

    authors: Horn H,Preclik G,Stange EF,Ditschuneit H

    更新日期:1991-08-01 00:00:00

  • Acute biliary pancreatitis: focus on recurrence rate and costs when current guidelines are not complied.

    abstract:BACKGROUND:International guidelines recommend cholecystectomy within 2-4 weeks after mild to moderate acute biliary pancreatitis (ABP) to prevent recurrence. We aimed to investigate the compliance to guidelines concerning early cholecystectomy and the associated costs. METHODS:Admissions for ABP 2011-2013 were retrosp...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2016.1243258

    authors: Ragnarsson T,Andersson R,Ansari D,Persson U,Andersson B

    更新日期:2017-03-01 00:00:00

  • Low-grade elevation of fibrinogen-degradation products is an important parameter to identify acute presentation of autoimmune hepatitis.

    abstract:OBJECTIVE:Diagnosing early-stage acute autoimmune hepatitis (AIH) without pathological findings is difficult. Recent reports indicated that macrophages are not activated during disease development, unlike in other acute liver injuries. We suggest that hepatitis without macrophage activation should lack sinusoidal fibri...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2016.1168865

    authors: Suzuki H,Harada S,Takao S,Takahashi M,Kato M,Kotoh K

    更新日期:2016-08-01 00:00:00

  • Helicobacter pylori CagA-positive strains affect oxygen free radicals generation by gastric mucosa.

    abstract:BACKGROUND:Helicobacter pylori plays a key role in production of reactive oxygen metabolites (ROMs). However, the importance of virulent CagA-positive H. pylori strains remains to be determined. The aim of this study was to assess ROMs production in gastric biopsies of patients infected by H. pylori. Results were corre...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.1080/003655201750074474

    authors: Danese S,Cremonini F,Armuzzi A,Candelli M,Papa A,Ojetti V,Pastorelli A,Di Caro S,Zannoni G,De Sole P,Gasbarrini G,Gasbarrini A

    更新日期:2001-03-01 00:00:00

  • S100A9 as a novel diagnostic and prognostic biomarker in human gastric cancer.

    abstract::Objective: The morbidity and mortality of gastric cancer (GC) is high, but there are lack of the biomarkers for early diagnosis and progression of GC. We aimed to identify a novel biomarker for the growth and progression of GC.Methods: The Cancer Genome Atlas (TCGA) database including 352 eligible patients was used to...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2020.1737883

    authors: Zhao Z,Zhang C,Zhao Q

    更新日期:2020-03-01 00:00:00

  • Gender, having a positive FIT and type of hospital are important factors for colonoscopy experience in colorectal cancer screening - findings from the SCREESCO study.

    abstract:OBJECTIVES:Assessing the experience of screening procedures is crucial for improving the quality and acceptance of colonoscopy in colorectal cancer screening. The aim of the study was to investigate the colonoscopy experience and associated factors among individuals who underwent a colonoscopy in the Screening of Swedi...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2020.1820568

    authors: Fritzell K,Forsberg A,Wangmar J,Wengström Y,Bottai M,Hultcrantz R

    更新日期:2020-11-01 00:00:00

  • Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility.

    abstract:OBJECTIVE:Many patients, especially women, suffer from severe gastrointestinal pain and dysmotility for several years without being diagnosed. Depletion of gonadotropin-releasing hormone (GnRH) in the enteric nervous system (ENS) has been described in some patients. The aim of this study was to examine the expression o...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2012.706826

    authors: Hammar O,Ohlsson B,Veress B,Alm R,Fredrikson GN,Montgomery A

    更新日期:2012-10-01 00:00:00

  • The effect of catecholamines on gastric mucosal flow.

    abstract::The effect of intravenous administration of adrenaline, noradrenaline, and isoprenaline on gastric mucosal flow, measured by hydrogen clearance techniques, showed that small doses of adrenaline induced slight mucosal vasodilatation in anaesthetized cats. In both anaesthetized and conscious animals both noradrenaline a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528209181076

    authors: Semb BK

    更新日期:1982-08-01 00:00:00

  • Mucosal cell proliferation in patients with hyperplastic colorectal polyps.

    abstract:BACKGROUND:Morphologic, histochemical, and cytoproliferative characteristics differentiate hyperplastic polyps from adenomas. Even so, these polyps are indicators of populations with a high colorectal cancer risk. Since changes in mucosal cell proliferation are highly predictive biomarkers of the adenomacarcinoma seque...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529509093288

    authors: Risio M,Arrigoni A,Pennazio M,Agostinucci A,Spandre M,Rossini FP

    更新日期:1995-04-01 00:00:00