Abstract:
:A simple, rapid, specific and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for the determination of doxycycline from the human plasma. Doxycycline is extracted from human plasma by solid phase extraction. Demeclocycline was used as an internal standard. Detection was performed at transitions of 444.800→428.200 for doxycycline and 464.700→448.100 for demeclocycline using mass spectrometry. Chromatographic separation of analyte and internal standard were carried out using a reverse phase C18, column at 0.500 ml/min flow. The assay of doxycycline is linear over the range of 0.055-7.612 μg/ml, with a precision <14.83%, regression coefficient (r(2))=0.9961 and the limit of quantification in plasma for doxycycline was 0.055 μg/ml. Mean extraction recovery obtained was 95.55%. Samples are stable at room temperature for 6 h, processed samples were stable at least for 30.20 h and also stable at three freeze-thaw cycles. The method has been used to perform pharmacokinetic and bioequivalence studies in human plasma.
journal_name
Indian J Pharm Scijournal_title
Indian journal of pharmaceutical sciencesauthors
Krishna AC,Sathiyaraj M,Saravanan RS,Chelladurai R,Vignesh Rdoi
10.4103/0250-474X.110599subject
Has Abstractpub_date
2012-11-01 00:00:00pages
541-8issue
6eissn
0250-474Xissn
1998-3743pii
IJPhS-74-541journal_volume
74pub_type
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