A novel and rapid method to determine doxycycline in human plasma by liquid chromatography tandem mass spectrometry.

Abstract:

:A simple, rapid, specific and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for the determination of doxycycline from the human plasma. Doxycycline is extracted from human plasma by solid phase extraction. Demeclocycline was used as an internal standard. Detection was performed at transitions of 444.800→428.200 for doxycycline and 464.700→448.100 for demeclocycline using mass spectrometry. Chromatographic separation of analyte and internal standard were carried out using a reverse phase C18, column at 0.500 ml/min flow. The assay of doxycycline is linear over the range of 0.055-7.612 μg/ml, with a precision <14.83%, regression coefficient (r(2))=0.9961 and the limit of quantification in plasma for doxycycline was 0.055 μg/ml. Mean extraction recovery obtained was 95.55%. Samples are stable at room temperature for 6 h, processed samples were stable at least for 30.20 h and also stable at three freeze-thaw cycles. The method has been used to perform pharmacokinetic and bioequivalence studies in human plasma.

journal_name

Indian J Pharm Sci

authors

Krishna AC,Sathiyaraj M,Saravanan RS,Chelladurai R,Vignesh R

doi

10.4103/0250-474X.110599

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

541-8

issue

6

eissn

0250-474X

issn

1998-3743

pii

IJPhS-74-541

journal_volume

74

pub_type

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