Epitope specificity of HLA class I alloantibodies: II. Stability of cross-reactive group antibody patterns over extended time periods.

Abstract:

:The stability of HLA alloantibodies was studied in 128 antibody-positive, potential kidney transplant recipients over an average period of 3 years. Antibody detection was performed using an anti-human globulin-complement-dependent cytotoxicity technique. In this study, the specificity of antibodies was categorized as against either private epitopes or cross-reactive group (CREG) epitope clusters. Definable antibodies were found in 94% of patients, and 89.5% of the definable antibodies had specificity for CREG clusters. Patterns of antibody reactivity were stable in most of the patients evaluated, even though the percentage of panel-reactive antibody (PRA) often demonstrated considerable fluctuations. Of the 220 definable private-specific or CREG cluster-specific antibodies identified in the patients, nearly 80% persisted throughout the observation period. The fluctuations in % PRA were common, but usually were not due to the acquisition of new HLA antibodies. Most fluctuations were attributable to variable detection of specificities within the same CREG cluster, possibly due to technique variation or changes in antibody avidity or titer or in cell panel composition. This study demonstrates that patterns of antibody specificity are remarkably stable in this patient population, even though PRA values fluctuated. This study further suggests that HLA antibody specificity analysis is a more useful clinical parameter of lymphocytotoxicity testing than simple reporting of % PRA when identifying potential donors for individual patients.

journal_name

Transplantation

journal_title

Transplantation

authors

Rodey GE,Revels K,Fuller TC

doi

10.1097/00007890-199703270-00015

subject

Has Abstract

pub_date

1997-03-27 00:00:00

pages

885-93

issue

6

eissn

0041-1337

issn

1534-6080

journal_volume

63

pub_type

杂志文章
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    abstract::We reviewed the results of all percutaneous fine needle aspirations (FNA) and open lung biopsies (OLB) after bone marrow transplantation at our center (1984-1987) for the evaluation of focal lung lesions that developed or persisted despite antibiotic administration. We sought to determine the prevalence and types of i...

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    pub_type: 杂志文章

    doi:10.1097/00007890-198908000-00017

    authors: Crawford SW,Hackman RC,Clark JG

    更新日期:1989-08-01 00:00:00

  • Ontogeny of immunoglobulins in bone marrow-transplanted individuals. An analysis of serum and salivary levels.

    abstract::Unstimulated whole saliva and serum samples were collected from ten allogeneic bone marrow transplant recipients before and after bone marrow transplantation, seven donors, and from twenty healthy individuals. Two patterns with regard to salivary IgA were found after transplantation. In five patients, a short-lasting ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198811000-00016

    authors: Norhagen-Engström G,Hammarström L,Lönnqvist B,Söder PO,Smith CI

    更新日期:1988-11-01 00:00:00

  • Insulin-heparin infusions peritransplant substantially improve single-donor clinical islet transplant success.

    abstract:BACKGROUND:Successful islet transplantation can result in insulin independence in many patients with type 1 diabetes mellitus, but it often requires more than one islet infusion. The ability to achieve insulin independence with a single donor is an important goal in clinical islet transplantation due to the limited org...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181c478fd

    authors: Koh A,Senior P,Salam A,Kin T,Imes S,Dinyari P,Malcolm A,Toso C,Nilsson B,Korsgren O,Shapiro AM

    更新日期:2010-02-27 00:00:00

  • Induction of transplantation tolerance in rats by spleen allografts. II. Evidence that W3/25+ T suppressor/inducer and OX8+ T suppressor/effector cells are required to mediate specific unresponsiveness.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198709000-00024

    authors: Stepkowski SM,Bitter-Suermann H,Duncan WR

    更新日期:1987-09-01 00:00:00

  • The role of donor-recipient relationship in long-term outcomes of living donor renal transplantation.

    abstract:BACKGROUND:Graft failure related to acute and chronic rejection remains an important problem in transplantation. An association has been reported between microchimerism and the development of tolerance. Since it has been established that cells of fetal origin can be found in maternal tissues long after parturition, and...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181705a0f

    authors: Miles CD,Schaubel DE,Liu D,Port FK,Rao PS

    更新日期:2008-05-27 00:00:00

  • Dose study of thymoglobulin during conditioning for unrelated donor allogeneic stem-cell transplantation.

    abstract:BACKGROUND:Thymoglobulin given before allo-hematopoietic stem-cell transplantation (HSCT) from unrelated donors reduces acute graft-versus-host disease (GvHD), but the optimal dose is unknown. METHOD:Four different doses of Thymoglobulin were given to 162 patients with hematologic malignancies undergoing unrelated don...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Remberger M,Svahn BM,Mattsson J,Ringdén O

    更新日期:2004-07-15 00:00:00

  • Inhibitor against coagulation factor V after liver transplantation.

    abstract::A new case of anti-factor V inhibitor is described in a 46-year-old man, who received a liver transplantation for hepatocellular carcinoma, without exposure to bovine thrombin or fibrin glue during the operative course. The inhibition occurred on the 14th postoperative day, while the patient was being treated with oxa...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199910150-00026

    authors: Leroy-Matheron C,Mallat A,Duvoux C,Métreau JM,Cherqui D,Dhumeaux D,Gouault-Heilmann M

    更新日期:1999-10-15 00:00:00

  • Intravenous infusion of Galalpha1-3Gal oligosaccharides in baboons delays hyperacute rejection of porcine heart xenografts.

    abstract:BACKGROUND:Hyperacute rejection (HAR) of pig-to-primate discordant xenografts is caused by the deposition of preexisting natural antibodies that recognize Galalpha1-3Gal (alphaGal)-terminating oligosaccharides on glycoproteins and glycolipids, followed by complement-mediated lysis of the graft's endothelium. In vitro, ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199802150-00009

    authors: Simon PM,Neethling FA,Taniguchi S,Goode PL,Zopf D,Hancock WW,Cooper DK

    更新日期:1998-02-15 00:00:00

  • Early enteral supply of lactobacillus and fiber versus selective bowel decontamination: a controlled trial in liver transplant recipients.

    abstract:BACKGROUND:Early enteral nutrition with solutions containing prebiotics (fiber) and probiotics (Lactobacillus) is suggested to reduce bacterial translocation and minimize the incidence of infections after liver transplantation. METHODS:In a prospective, randomized placebo-controlled trial consisting of 95 patients, we...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00007890-200207150-00021

    authors: Rayes N,Seehofer D,Hansen S,Boucsein K,Müller AR,Serke S,Bengmark S,Neuhaus P

    更新日期:2002-07-15 00:00:00

  • Association of chronic thromboxane inhibition with reduced in situ cytotoxic T cell activity in rejecting rat renal allografts.

    abstract::The development of allospecific cellular immunity during acute rat renal allograft rejection parallels alterations occurring in arachidonic acid metabolism, including increased production of the vasoconstrictor eicosanoid thromboxane A2 (TxA2). We have previously demonstrated that chronic inhibition of thromboxane syn...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Ruiz P,Coffman TM,Klotman PE,Sanfilippo F

    更新日期:1989-10-01 00:00:00

  • Over 500 solitary pancreas transplants in nonuremic patients with brittle diabetes mellitus.

    abstract:BACKGROUND:A pancreas transplant alone (PTA) in a nonuremic patient with brittle diabetes mellitus remains a rare procedure because the tradeoff for insulin independence is lifelong immunosuppression. METHODS:Herein we report our results at the University of Minnesota of 513 PTAs from December 17, 1966, through Decemb...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000296820.46978.3f

    authors: Gruessner RW,Sutherland DE,Kandaswamy R,Gruessner AC

    更新日期:2008-01-15 00:00:00

  • Humanization of Immunodeficient Animals for the Modeling of Transplantation, Graft Versus Host Disease, and Regenerative Medicine.

    abstract::The humanization of animals is a powerful tool for the exploration of human disease pathogenesis in biomedical research, as well as for the development of therapeutic interventions with enhanced translational potential. Humanized models enable us to overcome biologic differences that exist between humans and other spe...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000003177

    authors: Adigbli G,Ménoret S,Cross AR,Hester J,Issa F,Anegon I

    更新日期:2020-11-01 00:00:00

  • Immunobiology of xenogeneic cornea grafts in mouse eyes. II. Immunogenicity of xenogeneic cornea tissue grafts implanted in anterior chamber of mouse eyes.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200002270-00025

    authors: Tanaka K,Streilein JW

    更新日期:2000-02-27 00:00:00

  • "Striped" pattern of medullary ray fibrosis in allograft biopsies from kidney transplant recipients maintained on tacrolimus.

    abstract:BACKGROUND:A striped pattern of fibrosis has been described in the kidneys of patients undergoing long-term cyclosporine or tacrolimus therapy. This lesion is frequently misconstrued as being specific for drug toxicity. METHODS:We performed clinicopathologic correlation on 18 patients with striped fibrosis identified ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199902150-00025

    authors: Dell'Antonio G,Randhawa PS

    更新日期:1999-02-15 00:00:00

  • Effects of high-dose intravenous methylprednisolone on circulation in humans.

    abstract::We describe four patients who suffered asystole, myocardial infarction (in two cases) and supraventricular tachycardia at the time of high-dose methylprednisolone infusion. One patient died during infusion. We measured the effects of methylprednisolone and placebo infusion on heart rate, blood pressure, and cardiac ou...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章

    doi:10.1097/00007890-198304000-00017

    authors: Smith RS,Warren DJ

    更新日期:1983-04-01 00:00:00

  • A new micromethod for the detection of HL-A antigens on cultured human tumor cells.

    abstract::A rapid microcytotoxicity assay for the detection of HL-A antigens on tissue culture cells derived from human solid tumors is described. Tumor cells were prelabeled with 125Iododeoxyuridine. Isotopically labeled tumor cells were reacted with up to 37 highly selected HL-A antisera and diluted rabbit complement. Results...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-197509000-00005

    authors: Fritze D,Kern DH,Drogemuller CR,Pilch YH

    更新日期:1975-09-01 00:00:00

  • Knockdown of intraislet IKKβ by spherical nucleic acid conjugates prevents cytokine-induced injury and enhances graft survival.

    abstract:BACKGROUND:The efficiency of islet graft survival after intraportal implantation is compromised by host innate immune responses and the production of proinflammatory cytokines that cause acute cellular injury. This reaction activates intraislet nuclear factor-κB (NF-κB), causing production of gene products that have de...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3182a4190e

    authors: Rink JS,McMahon KM,Zhang X,Chen X,Mirkin CA,Thaxton CS,Kaufman DB

    更新日期:2013-11-27 00:00:00

  • Comparison of clinicopathologic characteristics of urothelial carcinoma between patients after renal transplantation and on dialysis.

    abstract:BACKGROUND:Urothelial carcinoma (UC) is a common complication after renal replacement therapy (RRT) among Chinese end-stage renal disease (ESRD) patients. It is unclear whether there are any differences in the clinicopathologic characteristics of UC between renal transplantation (RT) and dialysis patients; such differe...

    journal_title:Transplantation

    pub_type: 杂志文章,收录出版

    doi:10.1097/TP.0000000000000112

    authors: Zhang B,Shen C,Han WK,Yu W

    更新日期:2014-09-15 00:00:00

  • Effect of antithymocyte globulin on histocompatibility antigen recognition in man.

    abstract::Nonmajor histocompatibility complex (non-MHC) antigens are important targets of graft rejection and graft-versus-host disease in clinical transplantation. Little is known regarding immunity to non-MHC antigens. To study this problem we evaluated the effect of antihuman thymocyte globulin (ATG) on reactivity in autolog...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198102000-00001

    authors: Harada M,Gale RP

    更新日期:1981-02-01 00:00:00

  • Use of alemtuzumab and tacrolimus monotherapy for cadaveric liver transplantation: with particular reference to hepatitis C virus.

    abstract::We have proposed that the mechanisms of alloengraftment and variable acquired tolerance can be facilitated by minimum posttransplant immunosuppression. It was further suggested that the efficacy of minimalistic treatment could be enhanced by preoperative recipient conditioning with an antilymphoid antibody preparation...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000142674.78268.01

    authors: Marcos A,Eghtesad B,Fung JJ,Fontes P,Patel K,Devera M,Marsh W,Gayowski T,Demetris AJ,Gray EA,Flynn B,Zeevi A,Murase N,Starzl TE

    更新日期:2004-10-15 00:00:00

  • Contrasting in vivo effects on T helper cell functions induced by mitogenic (intact) versus nonmitogenic (F(ab')2) anti-CD3 monoclonal antibody.

    abstract::Anti-CD3 mAbs are potent inhibitors of T cell function; however, administration of mitogenic mAb can cause significant morbidity secondary to T cell activation and cytokine release. Nonmitogenic anti-CD3 mAb is immunosuppressive in mice without inducing detectable morbidity, and may thus be preferable for in vivo T ce...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Sawchuk SS,Gates R,Hirsch R

    更新日期:1995-12-15 00:00:00

  • Beneficial effect of pentoxifylline on microvesicular steatotic livers submitted to a prolonged cold ischemia.

    abstract:BACKGROUND:The deleterious effect of steatosis on transplanted livers is mainly related to a microcirculation impairment. We investigated the effect of preservation duration on the recovery of isolated perfused rat steatotic livers and tested the effect of pentoxifylline (PTX), known to have a beneficial effect on hepa...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.TP.0000071846.35825.B1

    authors: Arnault I,Bao YM,Sebagh M,Anjo A,Dimicoli JL,Lemoine A,Delvart V,Adam R

    更新日期:2003-07-15 00:00:00

  • Cell and extracellular matrix rejection in arterial concordant and discordant xenografts in the rat.

    abstract::Vascularized xenografts are rejected acutely and hyperacutely in concordant or discordant combinations, respectively. We investigated the impact of the donor-recipient combination on the rejection of arterial xenografts, analyzing the cellular and extracellular matricial compartments. Aortic xenografts were performed ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199609270-00017

    authors: Allaire E,Mandet C,Bruneval P,Bensenane S,Becquemin JP,Michel JB

    更新日期:1996-09-27 00:00:00

  • Pulmonary and blood stream infections in adult living donor and cadaveric liver transplant patients.

    abstract:BACKGROUND:Infectious complications occur in approximately 50% of cadaveric liver transplant (CDLT) recipients. Living-donor liver transplantation (LDLT) is an established alternative to shorten the waiting time. Currently, the incidence of pulmonary infections after LDLT and the microbiologic causes are unknown. In th...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e31816f61a6

    authors: Saner FH,Olde Damink SW,Pavlakovic G,van den Broek MA,Rath PM,Sotiropoulos GC,Radtke A,Canbay A,Paul A,Nadalin S,Malagó M,Broelsch CE

    更新日期:2008-06-15 00:00:00

  • A comparison of the clinical utility of the radioimmunoassay, high-performance liquid chromatography, and TDx cyclosporine assays in outpatient renal transplant recipients.

    abstract::Rapid and precise cyclosporine measurements are necessary to maximize immunosuppression and minimize toxicity. The new cyclosporine and metabolites TDx fluorescent polarization immunoassay was studied to determine its precision, sensitivity, and stability. Further, it was compared with the existing radioimmunoassay an...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198902000-00013

    authors: Schroeder TJ,Brunson ME,Pesce AJ,Hindenlang LL,Mauser PA,Ruckrigl DI,Weibel ML,Wadih G,First MR

    更新日期:1989-02-01 00:00:00

  • Analysis of factors affecting recurrence of hepatocellular carcinoma after liver transplantation with a special focus on inflammation markers.

    abstract:BACKGROUND:Systemic inflammation markers, such as neutrophil-to-lymphocyte ratio (NLR), have recently emerged as the prognostic factors for recurrence of liver tumors. METHODS:We assessed the ability of NLR and of other variables to predict the outcomes of orthotopic liver transplantation (OLT) for hepatocellular carc...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3182187cf0

    authors: Bertuzzo VR,Cescon M,Ravaioli M,Grazi GL,Ercolani G,Del Gaudio M,Cucchetti A,D'Errico-Grigioni A,Golfieri R,Pinna AD

    更新日期:2011-06-15 00:00:00

  • Release of platelet-activating factor from rabbit heart perfused in vitro by sera with transplantation alloantibodies.

    abstract::During "hyperacute rejection" of rabbit heart perfused with transplantation alloantibodies, platelet activating factor (PAF) was released into the coronary effluent, which appeared to have physicochemical and functional properties similar to the 1-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (synthetic PAF) and ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198707000-00023

    authors: Camussi G,Niesen N,Tetta C,Saunders RN,Milgrom F

    更新日期:1987-07-01 00:00:00

  • Liver preservation: a comparison of celsior to colloid-free University of Wisconsin solution.

    abstract:INTRODUCTION:Celsior (CEL) was formulated specifically for heart preservation. Recently some preliminary reports have suggested that CEL is also effective for liver preservation. In this study liver preservation with CEL was compared to colloid-free University of Wisconsin solution (MUW). METHODS:Arterialized rat live...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200010270-00002

    authors: Howden BO,Jablonski P

    更新日期:2000-10-27 00:00:00

  • Split Liver Transplantation and Pediatric Waitlist Mortality in the United States: Potential for Improvement.

    abstract:BACKGROUND:In the United States, 1 in 10 infants and 1 in 20 older children die on the liver transplant waiting list. Increasing split liver transplantation could increase organ availability for these children, without decreasing transplants in adults. METHODS:Using United Network for Organ Sharing Standard Transplant...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000002249

    authors: Perito ER,Roll G,Dodge JL,Rhee S,Roberts JP

    更新日期:2019-03-01 00:00:00

  • Postoperative oral glucose tolerance and stimulated insulin secretion: a predictor of endocrine graft function more than 10 years after pancreas-kidney transplantation.

    abstract:BACKGROUND:After pancreas transplantation, endocrine function is determined by the insulin secretory capacity of the transplanted pancreas. The authors evaluated the predictive value of postoperative oral glucose tolerance test (OGTT) and stimulated insulin secretion on long-term endocrine function. METHODS:Forty-one ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.TP.0000098821.26466.DE

    authors: Pfeffer F,Nauck MA,Drognitz O,Benz S,von Dobschuetz E,Hopt UT

    更新日期:2003-11-27 00:00:00