Abstract:
:The Polymerase Chain Reaction (PCR) is a highly innovative technique which allows for the generation of large amounts of DNA starting from minute quantities obtained from the blood or tissue of a patient. With the increasing knowledge concerning the structure of the human genome and the potential to amplify specific segments of DNA by the PCR technique, the molecular genetic characterization of many ocular disorders has been greatly facilitated. This is particularly true of retinoblastoma (RB) where the causative gene, RB1, gene has been identified and characterized. Using PCR technique, specific sequences of the RB1 gene can be amplified and analyzed to precisely define the genetic mutation in an affected individual. In addition, this technique can also be applied in order to characterize the genetic defect within the tumor itself. In this report we illustrate the use of the PCR technique in the genetic characterization of the RB1 gene and its application to the study of RB. These techniques are applicable even in a small clinical laboratory and can be extended to a number of ophthalmic disorders.
journal_name
Surv Ophthalmoljournal_title
Survey of ophthalmologyauthors
Mastrangelo D,Squitieri N,Bruni S,Hadjistilianou T,Frezzotti Rdoi
10.1016/s0039-6257(96)00004-5subject
Has Abstractpub_date
1997-01-01 00:00:00pages
331-40issue
4eissn
0039-6257issn
1879-3304pii
S0039-6257(96)00004-5journal_volume
41pub_type
杂志文章,评审abstract::An analytical review of the data available in the field of phakic intraocular lens implantation was conducted. Particular attention was paid to the more critical issues of intraocular lens sizing and safety guidelines. A comprehensive, competitive analysis of different implantation sites, intraocular lens model design...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2005.08.011
更新日期:2005-11-01 00:00:00
abstract::The court decisions included in this article reflect judicial attitudes towards medicolegal cases in most states. The courts have defined and amplified issues of informed consent, the physician's duty to warn patients and protect third parties, the limitations of the "Good Samaritan" statute, the responsibility of the...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(87)90071-3
更新日期:1987-07-01 00:00:00
abstract::Abnormal eye movements in the infant or voting child can be congenital or acquired. They may be a result of abnormal early visual development or a sign of underlying neurologic or neuromuscular disease. It is important to be able to detect these abnormalities and to distinguish them from normal but immature eye moveme...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(00)00114-4
更新日期:2000-05-01 00:00:00
abstract::Leiomyoma of the ciliary body is a rare tumor that often causes a diagnostic dilemma. Sclerouvectomy has been found to be beneficial in the management of iris and ciliary body leiomyoma. We treated a case of leiomyoma of the ciliary body presenting as a fleshy mass in the anterior chamber, removed by partial lamellar ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(99)00114-9
更新日期:2000-01-01 00:00:00
abstract::The need of blood flow to different organs varies rapidly over time which is why there is sophisticated local regulation of blood flow. The term dysregulation simply means that blood flow is not properly adapted to this need. Dysregulative mechanisms can lead to an over- or underperfusion. A steady overperfusion may b...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2007.08.010
更新日期:2007-11-01 00:00:00
abstract::A 20-year-old Hispanic man with Down syndrome presented with progressively worsening headache, fluctuating decreased vision, and bilateral optic disk edema. Magnetic resonance imaging of the head showed an empty sella, and magnetic resonance venography showed thrombosis of left transverse and sigmoid sinuses. Catheter...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2012.10.005
更新日期:2013-11-01 00:00:00
abstract::The only Confederate president, Jefferson Davis, led a long and eventful life. He was a Mississippi planter, a husband, a father, West Point graduate, war hero, congressman, senator, secretary of war, and finally President of the Confederate States of America. In many ways he was a study of contrast with his northern ...
journal_title:Survey of ophthalmology
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.survophthal.2006.08.007
更新日期:2006-11-01 00:00:00
abstract::Solitary fibrous tumor of the orbit is a rare spindle cell neoplasm. There are 42 cases of solitary fibrous tumor of the orbit available in the literature. We present six more cases of orbital solitary fibrous tumors, which presented to our institute between 1999 and 2001. We highlight the need for clinical recognitio...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(03)00087-0
更新日期:2003-09-01 00:00:00
abstract::Glaucoma, primarily a disease of the older population, may affect women of childbearing age. Pregnancy affects the intraocular pressure (IOP) of women with pre-existing glaucoma. Both elevations and reductions of IOP have been reported during pregnancy. Additionally, visual field test results may fluctuate during preg...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2010.11.008
更新日期:2011-07-01 00:00:00
abstract::William Ellergy Briggs was California's preeminent ophthalmologist for more than fifty years. A student of Ernst Fuchs, he was a clinician, scholar, and innovator who transplanted the best of European ophthalmology to the Western United States. ...
journal_title:Survey of ophthalmology
pub_type: 传,历史文章,杂志文章
doi:10.1016/0039-6257(90)90029-u
更新日期:1990-01-01 00:00:00
abstract::A 63-year-old man developed bilateral conjunctival injection and irritation followed by periocular discomfort and diplopia. Within weeks left proptosis and intermittent fever were noted. Orbital CT scan showed bilateral nodular enlargement of multiple extraocular muscles. Fine needle aspiration biopsy of the extraocul...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(88)90087-2
更新日期:1988-11-01 00:00:00
abstract::The light and electron microscopic findings are reviewed in two patients who had eyes enucleated for peripheral choroidal malignant melanomas. Preoperatively, cystoid macular edema was documented by fluorescein angiography in the melanoma-containing eye in both patients. Intracytoplasmic swelling (edema) of the Müller...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(84)90233-9
更新日期:1984-05-01 00:00:00
abstract::Retinitis pigmentosa is a clinically and genetically heterogeneous group of hereditary disorders in which there is progressive loss of photoreceptor and pigment epithelial function. The prevalence of retinitis pigmentosa is between 1/3000 and 1/5000 making it one of the most common causes of visual impairment in all a...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(88)90085-9
更新日期:1988-11-01 00:00:00
abstract::A melanocytoma (magnocellular nevus) is a benign pigmented tumor that may arise wherever uveal melanocytes are present. The most commonly observed site is on or adjacent to the optic nerve head. In the past this tumor was frequently misdiagnosed as a malignant melanoma and many unnecessary enucleations were carried ou...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(85)90107-9
更新日期:1985-03-01 00:00:00
abstract::Angiogenesis is a complex biologic process that occurs normally in development and in turnover and remodeling of mature vascular networks. Pathological angiogenesis and neovascularization occur in association with retinal and ocular ischemic diseases, in retinopathy of prematurity and other developmental disorders, an...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(96)82014-5
更新日期:1996-05-01 00:00:00
abstract::The possible applications of biofeedback to the treatment of ophthalmologic disorders is reviewed. Studies have suggested utility in the management of blepharospasms, strabismus, nystagmus, and other eye movement disorders as well as the training of voluntary control of accommodation. Limitations of the current litera...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(83)90195-9
更新日期:1983-05-01 00:00:00
abstract::Posterior scleritis must be considered in the differential diagnosis of many ocular conditions, including angle closure glaucoma, choroidal folds, optic disk edema, circumscribed fundus mass, choroidal detachment, and exudative retinal detachment. Because it is rare, a high index of suspicion is necessary. Anterior sc...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(88)90093-8
更新日期:1988-03-01 00:00:00
abstract::There are a number of unresolved questions regarding small choroidal melanomas. At what stage in the natural history of these tumors do they develop the capacity to metastasize? What are the effects of various therapeutic modalities on the incidence of metastatic disease? Clinicopathologic studies suggest that patient...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(78)90133-9
更新日期:1978-05-01 00:00:00
abstract:PURPOSE:The angiostatic cortisene anecortave acetate was evaluated in three safety and efficacy studies of patients with subfoveal choroidal neovascularization secondary to exudative age-related macular degeneration. METHODS:The Anecortave Acetate Monotherapy Trial enrolled 128 patients randomized to anecortave acetat...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2006.11.005
更新日期:2007-01-01 00:00:00
abstract::A 24-year-old woman with acute optic neuritis is presented. Appropriate management and treatment of this case using the Optic Neuritis Treatment Trial guidelines and an evaluation of the cost involved is discussed. ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(97)00117-3
更新日期:1998-01-01 00:00:00
abstract::A 56-year-old woman presented with a unilateral ptosis induced by a nonembedded soft contact lens of approximately 2 years' duration. The unilateral ptosis most likely resulted form localized inflammation and the physical presence of the soft contact lens. The patient's symptoms resolved completely after double lid ev...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(98)00029-0
更新日期:1998-09-01 00:00:00
abstract::Typical optic neuritis is an idiopathic demyelinating condition that is often associated with multiple sclerosis. This has been well characterized and has an excellent prognosis. Atypical optic neuritis can result from an inflammatory, infectious, or autoimmune disorder. Differentiating the two types of optic neuritis...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2019.06.001
更新日期:2019-01-01 00:00:00
abstract::For 11 years, a 50-year-old woman with euthyroid Graves' disease experienced intermittent exacerbations of her orbitopathy associated with a decline in visual acuity. On each occasion, treatment with systemic corticosteroids led to prompt recovery of vision. Upon referral for consideration for orbital decompressive su...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(97)00094-5
更新日期:1997-11-01 00:00:00
abstract::Age-related macular degeneration (AMD) involves a progressive impairment of the outer layers in the center of the retina. Experimental studies have demonstrated that bright light preferentially damages precisely the region that degenerates in AMD. The evidence that solar radiation is responsible for some of the deteri...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(88)90174-9
更新日期:1988-01-01 00:00:00
abstract::Age-related macular degeneration (AMD) is one of the leading causes of blindness worldwide with increasing prevalence owing to increased life expectancy. Intravitreal injections of antivascular endothelial growth factor agents are commonly used in exudative AMD and oral antioxidant medication for nonexudative AMD; how...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2020.08.005
更新日期:2020-09-21 00:00:00
abstract::With the use of special immune and enzymatic histochemical techniques, ocular pathologists are able to detect and localize certain cytoplasmic antigens and proteins in tissue section. An immunoperoxidase stain and special enzyme histochemical technique were used to confirm leukemic ocular involvement in a patient who ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(86)90081-0
更新日期:1986-09-01 00:00:00
abstract::Jules Gonin's former student presents a personal and historical biography of the Swiss ophthalmologist, who, between 1902 and 1921, explored and illustrated the many unknown aspects of retinal detachment, defined its pathogenesis, and developed the method of reattachment by ignipuncture and thermocautery. ...
journal_title:Survey of ophthalmology
pub_type: 传,历史文章,杂志文章
doi:10.1016/0039-6257(76)90125-9
更新日期:1976-11-01 00:00:00
abstract::Given the recent interest in complementary and alternative medicine (CAM), some patients may seek such treatments to supplement their traditional glaucoma management. The prevalence of CAM use for glaucoma is approximately 5%. We reviewed the literature to determine the potential benefit of various alternative treatme...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(01)00233-8
更新日期:2001-07-01 00:00:00
abstract::An elderly woman presented for evaluation of the recent onset of a fixed, blurry circle in the right eye. Visual acuity was 20/20 OU. Funduscopic examination revealed a newly-noticed isolated cotton-wool spot in each eye. Amsler grid testing revealed an arcuate shady area in the inferior nasal field. A thorough system...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(96)80072-5
更新日期:1996-03-01 00:00:00
abstract::A macular hole is a full-thickness defect of retinal tissue involving the anatomic fovea, thereby affecting central visual acuity. Macular holes have been associated with myriad ocular conditions and originally were described in the setting of trauma. The pathogenesis of idiopathic, age-related macular holes remains u...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(97)00132-x
更新日期:1998-03-01 00:00:00