Abstract：
:Polycystic kidney disease progresses more rapidly in men than in women. To investigate the basis for this sexual dimorphism, we exposed Madin-Darby canine kidney (MDCK) cells grown on collagen-coated cell culture inserts to control media, or to estradiol or testosterone (1 nM-1 microM). Compared to control and estradiol-treated cells, testosterone stimulated fluid secretion in a dose-dependent manner, enhancing fluid secretion 4.8-fold at 1 nM and 19.7-fold at 1 microM (0.59 +/- 0.18 vs. 0.03 +/- 0.01 microliter/cm2/hr, P < 0.001). Chloride transport paralleled fluid secretion. Testosterone increased cellular cyclic AMP levels 3.2-fold at 1 nM and 12.3-fold at 1 microM (81.3 +/- 30.7 vs. 6.6 +/- 3.3 pmol/mg protein, P < 0.001). GDP beta S (500 microM), an inhibitor of Gs, and 2',3'-dideoxyadenosine (10 microM), an inhibitor of the catalytic subunit of adenylate cyclase, suppressed testosterone-induced fluid and solute secretion. Neither testosterone nor estradiol had any effect on microsomal Na,K-ATPase activity, cellular proliferation or cellular total protein content. Our studies show that testosterone stimulates fluid secretion and solute transport by MDCK cells by increasing cAMP generation. In vivo, testosterone may contribute to cyst expansion by enhancing fluid secretion. This observation may help explain the worse prognosis of polycystic kidney disease observed in men.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Sandhu S,Silbiger SR,Lei J,Neugarten Jdoi
10.1038/ki.1997.211subject
Has Abstractpub_date
1997-05-01 00:00:00pages
1535-9issue
5eissn
0085-2538issn
1523-1755pii
S0085-2538(15)60051-5journal_volume
51pub_type
杂志文章abstract：BACKGROUND:In renal cells, hyperosmolarity has been shown to induce the accumulation of myo-inositol, via the Na+/myo-inositol cotransporter (SMIT). Previously we showed that SMIT mRNA in the kidney is localized in the medullary thick ascending limb of Henle (TALH). Here we used renal cells derived from the rabbit oute...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00235.x
更新日期：1999-01-01 00:00:00
abstract：:Diabetes mellitus is associated with natriuresis, whereas estrogen has been shown to be renoprotective in diabetic nephropathy and may independently regulate renal sodium reabsorption. The aim of this study was to determine the effects of 17-beta estradiol (E(2)) replacement to diabetic, ovariectomized (OVX) female ra...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000140
更新日期：2006-02-01 00:00:00
abstract：:Bikunin is a chondroitin-sulphate containing serum protein with a Stokes-Einstein radius and a negative net charge close to those of serum albumin. The plasma half life of bikunin is about 10 minutes, and approximately half of its clearance occurs in the kidneys. The quantitative role of glomerular filtration in the r...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.147
更新日期：1997-04-01 00:00:00
abstract：:We have sought to determine the mechanisms driving fluid secretion by the cystic epithelium in autosomal dominant polycystic kidney disease (ADPKD). We have performed in vitro experiments on intact cysts dissected from discarded ADPKD kidneys, on monolayers of cells cultured from the cystic epithelium and on microcyst...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1996.230
更新日期：1996-06-01 00:00:00
abstract：BACKGROUND:The superiority of renin-angiotensin system (RAS) blockade in providing renoprotection has been attributed to class-specific blood pressure "(BP)-independent" mechanisms. However, the conventional BP measurement methodology on which such conclusions are based is inherently limited for an accurate assessment ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00009.x
更新日期：2000-04-01 00:00:00
abstract：BACKGROUND:Urea kinetic modeling (UKM) and food records are widely used to assess the dialysis adequacy. Clinicians use these methods in individual patients to decide whether the dialysis prescription should be adjusted. We determined the variation in UKM parameters and dietary intake within individual patients in orde...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1046/j.1523-1755.1999.00412.x
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:Although the existence of multiple isoforms of plasma membrane calcium ATPase (PMCA) is now well documented, their biological functions are not yet known. In this study, we set out to investigate the potential role of PMCA isoforms, previously identified in renal cortical tissue, in tubular reabsorption of c...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00736.x
更新日期：1999-11-01 00:00:00
abstract：:The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.501
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:Immune response to oxidized low-density lipoprotein (oxLDL) may modulate the process of atherogenesis and cardiovascular disease. METHODS:We performed a prospective, observational cohort study in 249 patients with end-stage renal disease (ESRD) to examine whether the serum titer of anti-oxLDL antibody can p...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00692.x
更新日期：2002-12-01 00:00:00
abstract：:Previous studies from our laboratory have demonstrated that metabolic clearance rate (MCR) of calcitriol is decreased in experimental renal failure. In this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin D3 (25(OH)D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) on the MCR of calcitriol in renal ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.185
更新日期：1989-08-01 00:00:00
abstract：:Neutrophil gelatinase-associated lipocalin (Ngal) is a biomarker for acute and chronic renal injuries, including polycystic kidney disease (PKD). However, the effect of Ngal on PKD progression remains unexplored. To study this, we generated 3 strains of mice with different expression levels of Ngal within an establish...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.09.005
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:While the most common glomerular lesion associated with human immunodeficiency virus (HIV) infection is collapsing focal segmental glomerulosclerosis (FSGS) [HIV-associated nephropathy (HIVAN)], immune complex-mediated forms of glomerulonephritis have been increasingly reported. One form of glomerulonephriti...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00215.x
更新日期：2005-04-01 00:00:00
abstract：BACKGROUND:Regular monitoring of dialysis grafts is recommended, but the value of dialysis graft blood flow monitoring and venous pressures in predicting subsequent outcomes are controversial. METHODS:Over a period of one month, we performed simultaneous flow and venous pressure monitoring in 71 dialysis patients with...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1046/j.1523-1755.2001.060002762.x
更新日期：2001-08-01 00:00:00
abstract：UNLABELLED:A non-nucleotide-bridged DNA decoy inhibits renal epithelial nitric oxide synthase expression. BACKGROUND:The expression of inducible nitric oxide synthase (iNOS) is subject to strict tissue-specific transcriptional control. In mouse renal epithelium, an interferon-gamma (IFN-gamma)-induced signaling protei...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00809.x
更新日期：2000-01-01 00:00:00
abstract：:The renal effects of a single intravenous dose of two different E. coli lipopolysaccharides (LPS 0111:B4 and LPS 0127:B8), at the same dose of 100 micrograms/kg, were evaluated in euvolemic Munich-Wistar (MW) rats by whole kidney clearance techniques and micropuncture studies. Following LPS infusion, a significant dec...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.232
更新日期：1989-10-01 00:00:00
abstract：:A recent clinical trial in patients with chronic kidney disease (CKD) and diabetic nephropathy demonstrated that bardoxolone methyl (CDDO-ME) increases estimated glomerular filtration rate (eGFR) by an unknown mechanism. The paper by Ding et al. suggests that short-term administration of a CDDO-ME analog increases GFR...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2013.38
更新日期：2013-05-01 00:00:00
abstract：UNLABELLED:Predicting renal survival in primary focal glomerulosclerosis from the time of presentation. BACKGROUND:To predict the risk of developing chronic renal failure in patients with primary focal glomerulosclerosis (FGS) using predictors available at the time of presentation, a retrospective analysis was perform...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.4491164
更新日期：1999-12-01 00:00:00
abstract：:These studies were undertaken to examine effects of elevated glucose levels on glycolysis, sorbitol pathway activity, and the cytosolic redox state of NADH/NAD+ in isolated glomeruli. Blood-free glomeruli were isolated from kidneys of male, Sprague-Dawley rats using standard sieving techniques, then incubated for one ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.121
更新日期：1992-04-01 00:00:00
abstract：:Because the size of renal cysts in the native kidneys of patients with ADPKD who have been transplanted was found to be reduced when rapamycin was the immunosuppressant, we tested the involvement of the mTOR pathway in cyst enlargement. Here, male pcy mice, with mutation in one of the nephronophthisis genes, were trea...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.147
更新日期：2009-07-01 00:00:00
abstract：:During the heterologous phase of experimental anti-glomerular basement membrane (anti-GBM) nephritis, leukocyte influx peaks within hours, whereas albuminuria occurs within 1 day. In the subsequent autologous phase, endogenous anti-GBM IgG develops and albuminuria persists. Heparan sulfate (HS) proteoglycans like synd...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002514
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:Middle molecules such as beta2-microglobulin (beta2M) and advanced glycation end products (AGE)-modified proteins contribute to inflammation in uremia. The BetaSorb column is a new adsorptive device, which contains copolymeric beads, suitable for removal of beta2M and other middle molecules. We assessed the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00839.x
更新日期：2003-03-01 00:00:00
abstract：:Previous studies have shown that nonsteroidal anti-inflammatory drug (NSAID) use might be associated with a lower risk of developing cancer in the general population. Patients on dialysis have increased risk for cancer, but there are no studies to determine the relationship between NSAID use and cancer risk in these p...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2013.79
更新日期：2013-07-01 00:00:00
abstract：:Gain-of-function variants in CFB encoding factor B (FB), a component of the alternative pathway C3 convertase, have been reported in a minority of patients with aHUS and result in massive C3 activation. Zhang et al. describe the functional characterization of a novel FB variant (p.Ser367Arg) that they identified in 2 ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2020.06.026
更新日期：2020-11-01 00:00:00
abstract：:The inner medullary collecting duct (IMCD) is a major target site of atrial natriuretic peptide (ANP) for diuresis and natriuresis, and it is in a hypertonic condition made by the renal countercurrent multiplication system. We investigated the effects of hyperosmolality on ANP-stimulated cGMP generation in IMCD and gl...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.178
更新日期：1994-05-01 00:00:00
abstract：:The classic definition of hypercalciuria, an upper normal limit of 200 mg/day, is based on a constant diet restricted in calcium, sodium, and animal protein; however, random diet data challenge this. Here our retrospective study determined the validity of the classic definition of hypercalciuria by comparing data fro...
journal_title：Kidney international
pub_type： 杂志文章,meta分析,评审
doi：10.1038/ki.2011.227
更新日期：2011-10-01 00:00:00
abstract：:Atrial natriuretic peptide (ANP) is known to suppress platelet-derived growth factor (PDGF)-stimulated proliferation of rat cultured vascular smooth muscle cells. The present study examined whether ANP inhibits the PDGF receptor (PDGFR) tyrosine kinase activation, an initial event for PDGF cellular signaling. ANP redu...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.341
更新日期：1997-08-01 00:00:00
abstract：:Angiotensin converting enzyme (ACE) inhibitors are known to lower urinary protein excretion in human renal disease. This proteinuria lowering effect of ACE inhibition has been hypothesized to be a result of renal hemodynamic changes due to the inhibition of angiotensin II (Ang II) production. To test this hypothesis w...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1991.268
更新日期：1991-10-01 00:00:00
abstract：:A newly described glomerulotubular feedback loop may explain the relationship between glomerular damage, epitope spreading, tubulointerstitial nephritis, proteinuria as a progression factor, and the importance of the local milieu in kidney damage. It also opens the horizons for exciting innovative approaches to therap...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.489
更新日期：2010-03-01 00:00:00
abstract：:We explored how B-lymphocytes influence in vitro T-cell alloresponses in patients with antibody-mediated rejection (AMR), testing whether B-cells would be preferentially involved in this group of patients. Peripheral blood mononuclear cells were collected from 65 patients having biopsy: 14 patients with AMR and 5 with...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.100
更新日期：2015-09-01 00:00:00
abstract：:Monocyte chemoattractant protein 1 (MCP-1) is a C-C chemokine with potent monocyte chemotactic and activating properties that may contribute to glomerular macrophage infiltration in anti-GBM Ab GN. We have previously reported increased glomerular steady state expression of MCP-1 mRNA relative to GAPDH mRNA in the hete...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.363
更新日期：1996-08-01 00:00:00
