当前位置： SCI文献检索 > JOURNAL OF CATARACT AND REFRACTIVE SURGERY期刊下所有文献 > Corneal cell response after flap creation using a mechanical microkeratome or a 200 kHz femtosecond laser.

Corneal cell response after flap creation using a mechanical microkeratome or a 200 kHz femtosecond laser.

Abstract：

PURPOSE:To compare the inflammatory cell response within the corneal flap interface created by a mechanical microkeratome and a femtosecond laser. SETTING:Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. DESIGN:Experimental in vitro study. METHODS:Corneoscleral buttons of 12 enucleated human eyes not suitable for transplantation were put into organ culture. Corneal flaps were created using a 200 kHz femtosecond laser (Visumax) (femtosecond group) or a mechanical microkeratome (Amadeus) (microkeratome group). Flaps were not lifted after treatment. In 2 corneas, no treatment was performed (control group). Corneas were kept in organ culture for 12 hours thereafter. To evaluate cell-mediated immune reaction, immunofluorescent staining for leucocytes (cluster of differentiation 45) and specifically for dendritic cells (human leukocyte antigen-DR) was performed in every group. A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to determine apoptosis reaction. RESULTS:The ratio of dendritic cells in the femtosecond group compared with the microkeratome group was 1.2 (P=.02), the ratio of leucocytes was 1.4 (P=.06), and the ratio of apoptotic cells was 1.0 (P=.59). There was no marked significant difference in the distribution of inflammatory cell reaction. The control group showed neither specific inflammatory reaction nor apoptosis. CONCLUSION:This in vitro series of human corneas showed similar inflammatory tissue reaction after femtosecond laser-assisted and microkeratome-assisted flap creation (P<.05). FINANCIAL DISCLOSURE:No author has a financial or proprietary interest in any material or method mentioned.

journal_name

J Cataract Refract Surg

journal_title

Journal of cataract and refractive surgery

authors

Mayer WJ,Grueterich M,Wolf AH,Lackerbauer CA,Eibl K,Kampik A,Kook D

doi

10.1016/j.jcrs.2013.01.047

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

1088-92

issue

7

eissn

0886-3350

issn

1873-4502

pii

S0886-3350(13)00262-9

journal_volume

39

pub_type

杂志文章

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