Abstract:
:Vascular endothelial cells have been found to produce a relaxant mediator, identified as nitric oxide (NO) and implicated in numerous physiological functions. Subsequently, there has been an intensive search for accurate and specific detection methods to measure biological NO production. In the present study, we compared three approaches to evaluate NO production, based respectively on the Griess reaction (that quantifies nitrites and nitrates after their reduction), on the hemoglobin reaction (that quantifies oxyhemoglobin to methemoglobin transformation by NO), and on the electrochemical NO detection with a porphyrinic micro-probe. Comparison was made both under standard conditions and biological conditions, through calibration curves and measurements of histamine-induced NO production by cultured human endothelial cells and its modulation by L-arginine and N(omega)-monomethyl-L-arginine. We demonstrated that these three methods differ in terms of sensitivity and selectivity. The hemoglobin reaction and nitrate measurements suffer from a lack of specificity. Nitrite determination by the Griess reaction was hardly suitable for kinetic studies but it remains useful for the evaluation of basal NO production. The electrochemical technique, although it does not allow measurement of basal NO production, is the only one to exhibit great sensitivity and specificity and to allow instantaneous and non destructive measurements. This study brings up the potential hazards and pitfalls that may be associated with the various methods.
journal_name
Life Scijournal_title
Life sciencesauthors
Privat C,Lantoine F,Bedioui F,Millanvoye van Brussel E,Devynck J,Devynck MAdoi
10.1016/s0024-3205(97)00661-9subject
Has Abstractpub_date
1997-01-01 00:00:00pages
1193-202issue
12eissn
0024-3205issn
1879-0631pii
S0024320597006619journal_volume
61pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Utilizing a nine amino-acid (Asp-Glu-Leu-Tyr-Pro-Leu-Glu-Val-Glu) non-enkephalin containing fragment of Peptide F from the pro-enkephalin molecule, a radioimmunoassay was developed. Extraction of bovine, rat, and guinea pig adrenomedullary preparations demonstrated this fragment to be present and apparently partially ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90232-6
更新日期:1982-10-18 00:00:00
abstract::Rat aortic smooth muscle homogenates and membrane preparations contain a phospholipase C which hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2). We discovered that guanyl-5'yl-imidodiphosphate (Gpp(NH)p) activated the hydrolysis of exogenous PIP2 but not of phosphatidylinositol (PI) in rat aortic membranes. Furt...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90417-6
更新日期:1987-08-03 00:00:00
abstract::Intravenous (640 micrograms/kg) or intracerebroventricular (0.5 and 1 microgram) injection of Escherichia coli endotoxin (LPS) causes inhibition of water intake induced by 24 hour period of water deprivation in the rat. Tumor necrosis factor alpha (TNF-alpha; 20 and 40 ng/rat) given into the lateral cerebral ventricle...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90154-h
更新日期:1992-01-01 00:00:00
abstract::The lymphatic vessels have been implicated in maintenance of interstitial fluid homeostasis and immune responses, and pathological conditions including inflammation, wound healing, lymphedema and tumor progression. The knowledge about the lymphatic structure and function in muscles, especially in muscular disorders, r...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.09.043
更新日期:2018-10-15 00:00:00
abstract::The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02149-d
更新日期:1995-01-01 00:00:00
abstract:AIMS:Lithium is a common mood stabilizer to treat bipolar disorder. It has a narrow window of therapeutic action and its mechanism of action and possible side effects are still not fully understood. Lithium is a potent inhibitor of glycogen synthase kinase 3β (GSK-3β). Previous studies indicated that lithium can induce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.07.027
更新日期:2012-09-24 00:00:00
abstract::The potency of six antimuscarinic oxotremorine analogs at sympathetic ganglionic M1 and brainstem M2 muscarinic receptors was compared in the rat. Inhibition of the pressor effects of McNA343 or physostigmine was used as functional indicators of M1 and M2 blockade, respectively. 50% inhibitory doses (ID50) were calcul...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90442-t
更新日期:1990-01-01 00:00:00
abstract::Calcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in beta-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibiti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00152-6
更新日期:1999-01-01 00:00:00
abstract::This study examined the protective role of metallothionein (MT) against pulmonary damage caused by acute exposure to metallic mercury (Hg0) vapor using MT-null and wild-type mice. Both strains of mice were exposed to Hg0 at 6.6 to 7.5 mg/m3 for 4 hr each day for 3 consecutive days. This dosing protocol was lethal to o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00129-0
更新日期:1999-01-01 00:00:00
abstract::Specific binding sites for atrial natriuretic peptide (ANP) were identified and characterized in primary cultures of human thyroid cells. Saturation analysis using [125I] alpha rat ANP as the ligand showed a single class of high affinity binding (Kd = 0.2 nM) which was inhibited by atriopeptin I and the alpha -human f...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90172-0
更新日期:1989-01-01 00:00:00
abstract:AIMS:This study aims to explore the effect and underlying mechanism of zoledronic acid (ZA) on the incidence of thyroid cancer (TC) tumorigenesis. MATERIALS AND METHODS:Human mononuclear cells THP-1 were differentiated into M2-like tumor associated macrophages (TAMs) by incubation with PMA followed by additional incub...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117925
更新日期:2020-09-01 00:00:00
abstract::18-Hydroxycorticosterone (18-OHB) is a precursor of aldosterone and is the only corticosteroid, other than aldosterone, that is synthesized predominantly in the zona glomerulosa. Administration of the dopamine antagonist, metoclopramide results in parallel rises in plasma 18-OHB and aldosterone levels without affectin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90157-1
更新日期:1983-12-19 00:00:00
abstract::To study the effects of volume overload and renal failure on plasma levels of immunoreactive atrial natriuretic hormone (IR-ANH), we measured levels of this hormone in normal subjects, in patients with advanced chronic renal failure (CRF) with and without clinically evident volume overload, and in patients with end-st...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90732-6
更新日期:1987-09-28 00:00:00
abstract::The effects of agents that modulate intracellular release of calcium and protein kinase C (PKC) activation on noradrenaline (NA)-induced contractions of epididymal vas deferens in calcium-free/EGTA (1 mM) medium were investigated. NA (100 microM) or methoxamine (100 microM) evoked repeatable contractions. Clonidine (1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00231-3
更新日期:1999-01-01 00:00:00
abstract::We examined the effects of repeated administration of the selective serotonin uptake inhibitor (SSRI) fluoxetine (Flx) (5, 10, or 15 mg/kg i.p., twice daily for 21 days) on brain and plasma concentrations of the parent drug and its active desmethyl metabolite, norfluoxetine (NFlx), in rats during the 21-day regimen as...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00821-3
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Gastric cancer is a severe disease with a high occurrence rate worldwide. And lncRNAs are demonstrated to be responsible for cancer growth and metastasis. So, it is of great importance to explore the lncRNAs involved mechanism of gastric cancer occurrence and development deeply. METHODS:Transfection was con...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117478
更新日期:2020-05-15 00:00:00
abstract:AIM:To evaluate the therapeutic efficacy of hypoxic cell-sensitizer Sanazole (SAN) -directed targeting of cytotoxic drug Berberine (BBN) and Iron-oxide nanoparticle (NP) complexes, to solid tumor in Swiss albino mice. MAIN METHODS:NP-BBN-SAN complexes were characterized by FTIR, XRD, TEM and Nano-size analyzer. This c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.12.036
更新日期:2018-02-15 00:00:00
abstract::The aims of the current study were: 1) to determine if the epsilonPKC pseudosubstrate peptide (epsilonphi) (NH(2)-RKRQGAVRRRVHQVNG-COOH) could be used as an epsilonPKC-selective inhibitor in neonatal cardiac myocytes (NCMs) and 2) to determine if differences in the alpha and epsilonPKC autoinhibitory pseudosubstrate m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.11.016
更新日期:2004-05-07 00:00:00
abstract::Acute myocardial infarction (AMI) is associated with inflammation and apoptosis. Emodin plays an anti-inflammatory role in several inflammatory diseases. Recent studies have demonstrated that emodin protects against myocardial ischemia/reperfusion injury. However, its mechanism underlying its effects remains unknown. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.040
更新日期:2007-10-13 00:00:00
abstract::MSG (4 mg/g BW) or the equivalent molar ratio of NaCl were injected subcutaneously into chicks from four populations of chickens derived from a double selection experiment [(1) low exponential growth rate (EGR) to 14 days of age (14L), (2) high EGR to 14 days of age (14H), (3) low EGR to 42 days of age (42L) and high ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90061-f
更新日期:1991-01-01 00:00:00
abstract::Triiodothyronine (T3) increases phosphoglycerate mutase (PGAM) specific activity in rat skeletal and cardiac muscles. This increase is concomitant with an increase in the proportion of phosphoglycerate mutase isozymes which contain type-M subunit. Propylthiouracil (PTU), an anti-hormone, not only decreases phosphoglyc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90003-5
更新日期:1994-01-01 00:00:00
abstract:AIMS:DNA methylation and hydroxymethylation are significantly related to the occurrence and development of coronary heart disease (CHD) and atherosclerosis (AS). 5-Methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) are used to assess DNA methylation and hydroxymethylation levels, respectively. However, 5-mC and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.03.021
更新日期:2019-05-01 00:00:00
abstract:AIMS:Periodic acceleration (pGz) is a method that applies repetitive sinusoidal head-to-foot motion to the horizontally positioned body. pGz adds pulses to the circulation as a function of frequency, thereby increasing shear stress to the endothelium. Pulsatile shear stress increases release of cardioprotective endothe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.02.022
更新日期:2010-05-08 00:00:00
abstract::Preliminary data suggest that amygdalar corticotropin-releasing factor (CRF) is regulated by nicotinic agonists. We sought to confirm and extend these observations by determining the effects of various concentrations of nicotine on CRF messenger RNA expression in the AR-5 immortalized amygdalar cell line. Nicotine pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00539-1
更新日期:1999-01-01 00:00:00
abstract:AIMS:Poly (ADP-ribose) polymerase- (PARP)-1 is predominantly triggered by DNA damage. Overexpression of PARP-1 is known for its association with the pathogenesis of several CNS disorders, such as Stroke, Parkinson's disease (PD), Alzheimer's disease (AD), Huntington (HD) and Amyotrophic lateral sclerosis (ALS). NAD+ de...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118975
更新日期:2021-02-15 00:00:00
abstract::ACE and chymase play crucial roles in the establishment of pressure overload-induced cardiac hypertrophy. In the present study, time sequences of ACE and chymase-like activities, and their correlation with hypertrophic changes including free wall thickness and cardiac fibrosis, were elucidated in dogs with constant pr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.04.006
更新日期:2004-07-16 00:00:00
abstract::The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferred to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90472-9
更新日期:1988-01-01 00:00:00
abstract::The reproductive system effects of cocaine were studied in male rats. The analysis included measurements of circulating levels of luteinizing hormone (LH) and testosterone (T) by radioimmunoassay (RIA). The weights of the testes and sex accessory organs were also assessed and compared with control animals. Dosage leve...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90440-2
更新日期:1989-01-01 00:00:00
abstract::In order to investigate the action of ethanol on the anterior pituitary gland, the effect of ethanol on prolactin secretion in vitro was studied. Ethanol significantly increased the in vitro incorporation of 3H-leucine into both prolactin contained within the pituitary gland and that released into the medium. The enha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90473-9
更新日期:1984-11-05 00:00:00
abstract::This study was essentially an in vivo protection experiment designed to test further the hypothesis that stress induces release of endogenous opioids which then act at opioid receptors. Rats that were either subjected to restraint stress for 1 hr or unstressed were injected ICV with either saline or 2.5 micrograms of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90430-9
更新日期:1987-12-28 00:00:00