Abstract:
:Previous studies have demonstrated that calcium storage and release from IP3-dependent sites in the sarcoplasmic reticulum play an important role in the contractile response of the rabbit urinary bladder to both field stimulation (mediated via neurotransmitter release) and bethanechol (direct muscarinic stimulation). In view of the importance of SERCA (see text) in urinary bladder smooth muscle function, we studied the distribution of SERCA by two methods; using Western blotting to quantitate the protein concentration and by enzyme analysis using thapsigargin to specifically inhibit SERCA. Rabbit and human samples of urinary bladder smooth muscle were homogenized and the homogenate separate into three particulate fractions by different centrifugation: the cell wall-nuclear, mitochondrial, and microsomal. The protein concentration of these three particulate fractions was determined and the SERCA protein level quantitated by Western blotting using SERCA-2 antibodies. The calcium ATPase activity was quantitated using standard enzymatic analysis and the thapsigargin sensitivity determined. The results demonstrated that (1) the concentration of SERCA was significantly greater in the microsomal fraction than in either of the other fractions for both rabbit and human bladder smooth muscle; (2) the enzymatic activities of both total calcium-activated ATPase and thapsigargin-sensitive calcium ATPase were evenly divided among the three fractions, and (3) the enzymatic activity of both total calcium-activated ATPase and thapsigargin-sensitive calcium ATPase of the rabbit exceeded that of the human. In conclusion, the distribution of SERCA and calcium ATPase of the rabbit bladder smooth muscle was similar to that in the human bladder smooth muscle, although activities in rabbit were significantly greater than those of human tissue.
journal_name
Pharmacologyjournal_title
Pharmacologyauthors
Levin RM,Nicholas TJ,Snitkoff GG,Mandell J,Russell D,Wilbur HJ,Mogavero LJdoi
10.1159/000139543subject
Has Abstractpub_date
1997-12-01 00:00:00pages
309-16issue
6eissn
0031-7012issn
1423-0313journal_volume
55pub_type
杂志文章相关文献
PHARMACOLOGY文献大全abstract::We studied the action of beta-adrenergic agonists on Japanese quail erythrocyte carbonic anhydrase (CA) in vitro. Earlier we had reported that epinephrine increased CA activity by 14%; the present study focused on an attempt to increase the size of this response. Washed erythrocytes from reserpine-treated (1 mg/kg dai...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139223
更新日期:1994-08-01 00:00:00
abstract::Two groups of rats were used to examine the effect of pioglitazone, a peroxisome proliferator-activated receptor gamma (PPARgamma) agonist, on rat hearts using an in vivo model of ischemia-reperfusion (I/R) to elucidate potential mechanisms. One group was the 30-min reperfusion group, which was further subdivided into...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000100870
更新日期:2007-01-01 00:00:00
abstract::The regulatory action exerted on receptors by acebutolol, a cardioselective beta-blocker containing intrinsic sympathomimetic activity, has been investigated. beta-Adrenoceptor affinity and density of human mononuclear leukocytes were assayed in hypertensive patients before and after treatment with 400 mg/day acebutol...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138132
更新日期:1985-01-01 00:00:00
abstract::Fructose-1,6-diphosphate (FDP) was found to cause significant stimulation of nitric oxide synthase (NOS) in rat liver homogenates in vitro. This effect was more pronounced for the inducible isoform than its constitutive counterpart. Furthermore, FDP restored rat liver inducible NOS levels following their depletion by ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139468
更新日期:1997-01-01 00:00:00
abstract::A comparison was made of the anti-ischemic effects of dihydropyridine calcium antagonists in isolated globally ischemic rat hearts. Pretreatment with amlodipine, nifedipine, nitredipine, or nisoldipine reduced reperfusion enzyme (lactate dehydrogenase) release and contracture after 25 min of global ischemia and 30 min...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138652
更新日期:1990-01-01 00:00:00
abstract::Sarcopenia is a geriatric syndrome characterized by progressive and generalized loss of skeletal muscle mass and function. Reported prevalence of this geriatric syndrome, differs depending on the definition, the population and the method used to identify sarcopenia. The causes of sarcopenia are multifactorial, and can...
journal_title:Pharmacology
pub_type: 杂志文章,评审
doi:10.1159/000448247
更新日期:2017-01-01 00:00:00
abstract::Veratridine caused a positive inotropic action in the electrically driven left atria of rats. Quinacrine (a phospholipase A2 inhibitor), indomethacin (a cyclooxygenase inhibitor) and aspirin (a cyclooxygenase inhibitor), but not nordihydroguaiaretic acid (a lipoxygenase inhibitor), inhibited the response to veratridin...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138774
更新日期:1991-01-01 00:00:00
abstract::The antagonism of carbachol-induced contractions of guinea pig gallbladder smooth muscle strips via selective antagonists, methoctramine, HHSiD, pf-HHSiD and DABDMA has been investigated in order to find out the m-cholinoceptor subtype(s) of gallbladder smooth muscle. All m-cholinoceptor antagonists examined, displace...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139061
更新日期:1993-06-01 00:00:00
abstract::The temporal effects of vitamin A deficiency on hepatic cytochrome P-450-dependent and conjugation reactions were studied in the rat. Cytochrome P-450 levels and N-methyl-p-chloroaniline N-demethylase activity were significantly reduced in the deficient animals. No other changes in parameters dependent on cytochrome P...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137457
更新日期:1980-01-01 00:00:00
abstract::DL-o-, and p-octopamine were tested for beta- and alpha-adrenergic activity in rats. When compared to DL-isoproterenol, a beta-adrenergic agonist, all three isomers failed to show significant beta-adrenergic activity as assessed by intiation of thirst and by increase in tail skin temperature. All three isomers increas...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137250
更新日期:1979-01-01 00:00:00
abstract::The objective of this study was to determine the effects of hypercholesterolemia on basal vascular tone and vascular responses to pharmacologic agents in hindquarter resistance vessels. Blood pressure and hindquarter blood flow were measured in conscious rabbits fed a high cholesterol diet (1%) for 17 weeks (HC) compa...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000056137
更新日期:2001-01-01 00:00:00
abstract::The present study was carried out to test bioequivalence between two different oral desmopressin formulations. Sixty healthy volunteers were enrolled in the study and were randomly assigned to receive the test (T) and reference (R) drug in a two-period two-sequence, crossover, analyst-blinded study design. Subjects re...
journal_title:Pharmacology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000092625
更新日期:2006-01-01 00:00:00
abstract::Clofibrate treatment decreased drastically the activity of serum postheparin lipoprotein lipase of rats fed with fat-free test diet but had no effect on hepatic lipase. 3 weeks' treatment was followed by a significant fall in serum cholesterol caused by a marked decrease in HDL-cholesterol. Clofibrate decreased in vit...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137813
更新日期:1983-01-01 00:00:00
abstract::Angiotensin II (ANG II) is a potent vasoconstrictor in most vascular beds. We studied the role of cyclooxygenase products and/or endothelium-derived relaxing factor (EDRF) in modulating the pressor response to ANG II in the isolated, blood perfused dog lung. ANG II was given as a bolus dose of 2, 4 and 8 micrograms be...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138709
更新日期:1990-01-01 00:00:00
abstract::The effects of cyclic adenosine monophosphate-dependent protein kinase (PKA) and calcium-dependent protein kinase (PKC) modulators on secretagogue-stimulated gastric acid secretion were studied in the continuously perfused stomach of the anesthetized rat. Intravenous histamine (0.25 mg/kg/h) and pentagastrin (2 microg...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139368
更新日期:1995-10-01 00:00:00
abstract::The present study examined the interaction of opioidergic systems in the hypotensive action of atenolol. Anesthetized, adult spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) rats were instrumented to monitor blood pressure and heart rate. Atenolol (100 micrograms/kg, i.v.) produced a decrease in blood pressure ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138585
更新日期:1989-01-01 00:00:00
abstract::Several experiments suggested that vaccines might inhibit liver drug-metabolizing enzymes, an assumption in agreement with other findings obtained with most immuno-enhancing agents. A direct, non-specific activation of macrophages with subsequent release of interleukin 1 may account for this inhibition. The purpose of...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138370
更新日期:1988-01-01 00:00:00
abstract::Oxygen radicals have been considered to be important regulatory molecules in Helicobacter pylori-induced gastric ulceration and carcinogenesis. H. pylori-induced inflammation has been shown to be associated with cyclooxygenase-2 (COX-2) expression in experimental animals and human patients. This study aimed to determi...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000065532
更新日期:2002-12-01 00:00:00
abstract::Altered vascular reactivity due to endothelial dysfunction, consequent to vascular damage, is observed in rheumatoid arthritis. We investigated the effect of angiotensin (Ang)-(1-7) on vasculature changes in arthritis induced by complete Freund's adjuvant in male Wistar rats. Arthritis decreased soluble receptor for a...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000444188
更新日期:2016-01-01 00:00:00
abstract::A rapid and sensitive gas chromatographic method for the determination of the beta-adrenergic agent isoxsuprine in human plasma has been developed. The procedure involves the extraction of the drug with ether and an internal standard (propranolol) from plasma at alkaline pH, solvent evaporation, and the formation of a...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137843
更新日期:1983-01-01 00:00:00
abstract::Imatinib and AMN107 are protein tyrosine kinase inhibitors which reduce KIT autophosphorylation with similar potency. This report describes the cellular uptake of these compounds in two human gastrointestinal stromal tumor (GIST)-derived cell lines (GIST882 and GIST GDG1), which both express constitutively activated K...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000091943
更新日期:2006-01-01 00:00:00
abstract::The present study was designed to examine the reaction pathway of colloidal bismuth subcitrate (CBS) with thiols. Studies were performed using the monothiol glutathione (GSH), the dithiol dithiothreitol (DTT) and the thiol enzymes papain and H+/K(+)-ATPase. UV-vis spectra showed that CBS forms complexes with GSH and D...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139088
更新日期:1993-08-01 00:00:00
abstract::Both in vivo and in vitro exposure to morphine have been reported to produce a number of immunomodulatory effects in both laboratory animals and humans. The current study was performed to assess the direct in vitro effect of exposure to morphine or morphine metabolites on immune response parameters. Murine B6C3F1 sple...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139266
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effects of trichostatin A (TSA) on cervical cancer and the related mechanisms. METHODS:The HeLa and Caski cervical cancer cell lines were treated with different concentrations of TSA. Cell viability was measured by MTT assays. Cell apoptosis was analysed using flo...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000507937
更新日期:2020-11-03 00:00:00
abstract:AIMS:The aim of the present study was to investigate the effects of rutin on potassium oxonate-induced hyperuricemia and renal dysfunction in mice. METHODS:Rutin was administered orally 1 h after oxonate at doses of 25, 50 and 100 mg·kg(-1). Serum uric acid levels and kidney function parameters were assayed. Mouse uro...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000351703
更新日期:2013-01-01 00:00:00
abstract::The aim of this study was to compare the effects of a new thromboxane synthase inhibitor, camonagrel, on platelet aggregation and platelet-subendothelium interaction under flow conditions, in comparison with a standard thromboxane synthase inhibitor (dazoxiben) and a cyclooxygenase inhibitor (acetylsalicylic acid). Wi...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000071266
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND/AIMS:In this study, we evaluated the functional impact of facilitatory presynaptic adenosine A2A and muscarinic M1 receptors in the recovery of neuromuscular tetanic depression caused by the blockage of high-affinity choline transporter (HChT) by hemicholinium-3 (HC-3), a condition that mimics a myasthenia-l...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000494058
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Putative in vitro-in vivo correlations of pharmacokinetic (PK) parameters are regarded as a prerequisite to filter hits derived from high-throughput screening (HTS) approaches for subsequent murine in vivo PK studies. METHODS:In this study, we assessed stabilities in rat and human microsomes of 121 com...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000343241
更新日期:2012-01-01 00:00:00
abstract::The effect of repeated administration of U-50,488H, a kappa-opioid receptor agonist, on the development of tolerance to its analgesic effect and on the 5-HT1 and 5-HT2 receptors in the cerebral cortex and spinal cord of the rat were determined. Male Sprague-Dawley rats were injected twice daily with U-50,488H, (25 mg/...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138591
更新日期:1989-01-01 00:00:00
abstract::L-Carnitine (oral route) significantly corrects the muscle hypocontractility and hypoexcitability induced in the rat after 5 consecutive days of fasting. This effect is interpreted on the basis of the dual role of L-carnitine as cofactor in the transport of long-chain fatty acids into the mitochondria and as a detoxif...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139026
更新日期:1993-01-01 00:00:00