Abstract:
:Reprogramming of somatic cells to desired cell types holds great promise in regenerative medicine. However, production of transplantable hematopoietic stem cells (HSCs) in vitro by defined factors has not yet been achieved. Therefore, it is critical to fully understand the molecular mechanisms of HSC development in vivo. Here, we show that Fev, an ETS transcription factor, is a pivotal regulator of HSC development in vertebrates. In fev-deficient zebrafish embryos, the first definitive HSC population was compromised and fewer T cells were found in the thymus. Genetic and chemical analyses support a mechanism whereby Fev regulates HSC through direct regulation of ERK signaling. Blastula transplant assay demonstrates that Fev regulation of HSC development is cell autonomous. Experiments performed with purified cord blood show that fev is expressed and functions in primitive HSCs in humans, indicating its conserved role in higher vertebrates. Our data indicate that Fev-ERK signaling is essential for hemogenic endothelium-based HSC development.
journal_name
Bloodjournal_title
Bloodauthors
Wang L,Liu T,Xu L,Gao Y,Wei Y,Duan C,Chen GQ,Lin S,Patient R,Zhang B,Hong D,Liu Fdoi
10.1182/blood-2012-10-462655subject
Has Abstractpub_date
2013-07-18 00:00:00pages
367-75issue
3eissn
0006-4971issn
1528-0020pii
blood-2012-10-462655journal_volume
122pub_type
杂志文章相关文献
BLOOD文献大全abstract::The combination of imatinib with chemotherapy has been recently reported as very promising in patients with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). During 2004 and 2005, 45 patients with newly diagnosed Ph+ ALL were treated in the Group for Research on Adult Acute Lymphoblastic Leuke...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2006-03-011908
更新日期:2007-02-15 00:00:00
abstract::Phosphoinositide 3-kinase gamma (PI3Kgamma) in neutrophils plays a critical role in the directed migration of these cells into inflamed tissues. In this study, we demonstrate the importance of the endothelial component of PI3Kgamma activity relative to its leukocyte counterpart in supporting neutrophil interactions wi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-01-0023
更新日期:2005-07-01 00:00:00
abstract::The effect of lipopolysaccharide (LPS) on endothelial cells is a key component of the inflammatory response seen in Gram-negative sepsis. LPS does not cause death of cultured human endothelial cells. However, when the expression of new proteins is inhibited by cycloheximide, microvascular endothelial cells in culture ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-10-15 00:00:00
abstract::Notch signaling has been shown to play a key role in cell fate decisions in numerous developmental systems. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we reported the expression of human Notch-1 in CD34+ progenitors. In this study, we evaluated the expression of human Notch-1 and Notch-2 p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Polymorphonuclear neutrophils (PMN) contain multiple distinct secretory compartments that are sequentially mobilized during cell activation. Complement receptor type 1 (CR1) is a marker for a readily mobilizable secretory vesicle compartment, which can undergo exocytic fusion with the plasma membrane independently of ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::The last 2 years have seen much excitement in the field of genetics with the identification of a formerly unappreciated level of "structural variation" within the normal human genome. Genetic structural variants include deletions, duplications, and inversions in addition to the recently discovered, copy number variant...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2006-06-030858
更新日期:2007-02-15 00:00:00
abstract::The relationship between the level of retinoblastoma protein (RB) expression and the survival of 113 newly diagnosed acute myelogenous leukemia (AML) patients was studied. Western blotting was used to determine the level of RB protein present in peripheral blood leukemia cells and results were confirmed in 26 patients...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::The granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin (IL)-3/IL-5 receptor family regulates the production and function of myeloid cells. These cytokines signal through receptor complexes that consist of unique ligand-binding alpha-chains and common signaling beta-chains. IL-5 is distinct from IL-3...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-03-208850
更新日期:2009-10-29 00:00:00
abstract::We evaluated 18,014 patients who underwent allogeneic bone marrow transplantation (BMT) at 235 centers worldwide to examine the incidence of and risk factors for posttransplant lymphoproliferative disorders (PTLD). PTLD developed in 78 recipients, with 64 cases occurring less than 1 year after transplantation. The cum...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:
更新日期:1999-10-01 00:00:00
abstract::T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignancy that accounts for ∼20% of ALL cases. Intensive chemotherapy regimens result in cure rates >85% in children and <50% in adults, warranting a search of novel therapeutic strategies. Although immune-based therapies have tremendously improved the trea...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003801
更新日期:2020-09-10 00:00:00
abstract::The regulator of G-protein signaling (RGS) negatively regulates the alpha subunit of G proteins by accelerating their intrinsic guanosine triphosphatase (GTPase) activity. Here are reported the isolation and characterization of a novel mouse RGS, termed RGS18, which is a new member of RGS subfamily B. Northern blot an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.10.3051
更新日期:2001-05-15 00:00:00
abstract::Transplantation-associated thrombotic microangiopathy (TA-TMA) is a challenging diagnosis after hematopoietic stem cell transplantation. Although endothelial injury represents the final common pathway of disease, the exact pathophysiology of TA-TMA remains unclear. Potential causes include infections, chemotherapy, ra...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-02-321315
更新日期:2011-08-11 00:00:00
abstract::Hepcidin is the master regulator of iron homeostasis. In the liver, iron-dependent hepcidin activation is regulated through Bmp6 and its membrane receptor hemojuvelin (Hjv), whereas, in response to iron deficiency, hepcidin repression seems to be controlled by a pathway involving the serine protease matriptase-2 (enco...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-07-295147
更新日期:2011-01-13 00:00:00
abstract::Ibrutinib is highly efficacious and used at 420 mg/d for treatment of chronic lymphocytic leukemia (CLL). We previously demonstrated a decline in Bruton's tyrosine kinase (BTK) protein levels in CLL cells after 1 cycle of ibrutinib, suggesting ibrutinib dose could be lowered after the first cycle without loss of biolo...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2018-06-860593
更新日期:2018-11-22 00:00:00
abstract::Localized tumors composed of immature cells of the myelogenous series have been recognized for many years as an uncommon manifestation of granulocytic leukemia. The histologic diagnosis of chloroma (granulocytic sarcoma) may be extremely difficult when the myeloblastic cells are poorly differentiated and the tumor lac...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::The TEL/PDGFbetaR fusion protein is expressed as the consequence of a recurring t(5;12) translocation associated with chronic myelomonocytic leukemia (CMML). Unlike other activated protein tyrosine kinases associated with hematopoietic malignancies, TEL/PDGFbetaR is invariably associated with a myeloid leukemia phenot...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Between February 1986 and March 1990, 56 patients with relapsed Hodgkin's disease treated with high-dose cyclophosphamide, carmustine, and etoposide (CBV) received an autologous peripheral stem cell transplantation (PSCT) rather than an autologous bone marrow transplantation (ABMT) because each patient had a marrow ab...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The tetraspanin family member CD151 forms complexes with integrins and regulates cell adhesion and migration. While CD151 is highly expressed in megakaryocytes and to a lesser extent in platelets, its physiologic role in platelets is unclear. In this study, we investigate the physical and functional importance of CD15...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-12-4430
更新日期:2004-10-15 00:00:00
abstract::Previous results have demonstrated that anti-D therapy in children with chronic auto-immune thrombocytopenic purpura (AITP) induced a significant increase in several pro- and anti-inflammatory plasma cytokines within 2 hours of administration. To investigate the biologic basis of these early in vivo responses, we deve...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-04-1029
更新日期:2003-10-15 00:00:00
abstract::Myelodysplastic and leukemic stem cell clones that evolve in children and adults with Fanconi anemia universally bear complex cytogenetic abnormalities. The abnormalities are generally recurring deletions or chromosomal loss and involve precisely the same chromosomes with the same frequency as has been described in ma...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-09-2781
更新日期:2003-07-01 00:00:00
abstract::Most patients with chronic lymphocytic leukemia (CLL) are diagnosed with early-stage disease and managed with active surveillance. The individual course of patients with early-stage CLL is heterogeneous, and their probability of needing treatment is hardly anticipated at diagnosis. We aimed at developing an internatio...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003453
更新日期:2020-05-21 00:00:00
abstract::D assemblies make up half of the von Willebrand factor (VWF), yet are of unknown structure. D1 and D2 in the prodomain and D'D3 in mature VWF at Golgi pH form helical VWF tubules in Weibel Palade bodies and template dimerization of D3 through disulfides to form ultralong VWF concatemers. D'D3 forms the binding site fo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-10-876300
更新日期:2019-04-04 00:00:00
abstract::The combination of cyclophosphamide, vincristine, and prednisone (CVP) is one of several standard treatment options for advanced follicular lymphoma. This, like similar chemotherapeutic regimens, induces response rates of 60% to 80%, with a median response duration of under 2 years. Rituximab, a chimeric monoclonal an...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2004-08-3175
更新日期:2005-02-15 00:00:00
abstract::Stem cell factor (SCF) binding to the c-kit receptor triggers homodimerization and intermolecular tyrosine phosphorylation of the c-kit receptor, thus initiating signal transduction. Receptor dimerization is a critical early step in this process. Prior biochemical studies of c-kit receptor dimerization have mainly use...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::The effective activation of factor X by factor IXa requires the co-factor activity of activated factor VIII (FVIII). Factor Xa formation is also dependent on the presence of negatively charged phospholipid. A phospholipid binding domain of FVIII has been reported to be present on the FVIII light chain. Recent observat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-05-15 00:00:00
abstract::Peripheral T-cell lymphomas (PTCLs) are aggressive tumors in which the current therapy based on multiagent chemotherapy is not successful. Since cytochrome P450 3A subfamily (CYP3A) enzymes are involved in the inactivation of chemotherapy drugs, we hypothesized that CYP3A and P-glycoprotein (MDR1) expression in these ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-02-075036
更新日期:2007-11-01 00:00:00
abstract::Fanconi anemia (FA) is an autosomal recessive syndrome featuring diverse symptoms including progressive bone marrow failure and early occurrence of acute myeloid leukemia. Nine genetic subtypes have been described for FA (A, B, C, D1, D2, E, F, G, and L), all of which have been connected to distinct disease genes, exc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-08-2915
更新日期:2004-04-01 00:00:00
abstract::Normal human erythroid cell maturation requests the transcription factor GATA-1 and a transient activation of caspase-3, with GATA-1 being protected from caspase-3-mediated cleavage by interaction with the chaperone heat shock protein 70 (Hsp70) in the nucleus. Erythroid cell dysplasia observed in early myelodysplasti...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-03-343475
更新日期:2012-02-09 00:00:00
abstract::Erythroid Kruppel-like factor (EKLF) is a transcription factor of the C2H2 zinc-finger class that is essential for definitive erythropoiesis. We generated immortal erythroid cell lines from EKLF(-/-) fetal liver progenitor cells that harbor a single copy of the entire human beta-globin locus and then reintroduced EKLF...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.6.1861
更新日期:2001-03-15 00:00:00
abstract::Radioimmunotherapy (RIT) has emerged as an effective treatment for lymphoma, however the underlying mechanisms are poorly understood. We therefore investigated the relative contributions of antibody and targeted radiation to the clearance of tumor in vivo, using 2 different syngeneic murine B-cell lymphoma models. Alt...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-06-2037
更新日期:2004-02-15 00:00:00