Abstract:
:The membrane fusion events observed during neutrophil degranulation are important aspects of the immunoregulatory system. In an attempt to understand the regulation of granule-plasma membrane fusion, we have begun characterizing human neutrophil cytosol for fusion activity, finding that 50% of the fusogenic activity could be attributed to members of the annexin family of proteins. The major non-annexin fusion activity (25% of the total cytosolic activity) was enriched by ion exchange chromatography after depletion of annexins by Ca2+-dependent phospholipid affinity chromatography. The fusion activity co-purified with a 10,14-kDa dimer identified as leukocyte L1 (which was non-fusogenic), along with an approximately 36-kDa protein. This protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by amino-terminal sequencing, and the fusion activity was verified using commercially available GAPDH. GAPDH may play an important role in degranulation because it is as potent as annexin I on a mass basis and may constitute up to 25% of the total cytosolic fusion activity of the neutrophil.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Hessler RJ,Blackwood RA,Brock TG,Francis JW,Harsh DM,Smolen JEdoi
10.1002/jlb.63.3.331subject
Has Abstractpub_date
1998-03-01 00:00:00pages
331-6issue
3eissn
0741-5400issn
1938-3673journal_volume
63pub_type
杂志文章abstract::CXC chemokines, which induce angiogenesis, have glutamine-leucine-arginine amino acid residues (ELR motif) in the amino terminus and bind CXCR2 and the Duffy antigen chemokine-binding protein. Duffy, a seven transmembrane protein that binds CXC and CC chemokines, has not been shown to couple to trimeric G proteins or ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The mannose receptor is a macrophage surface receptor that mediates both endocytosis and phagocytosis. Previous work has demonstrated that the prototypical Th 2 cytokine, interleukin-4 (IL-4), increases both cell-surface receptor expression and mannose receptor-mediated endocytosis, whereas the prototypical Th 1 cytok...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.64.1.108
更新日期:1998-07-01 00:00:00
abstract::Human peritoneal macrophages (HPM) obtained via laparoscopy were examined for the presence and functional capacity of complement and Fc receptors. Between 5 and 20 ml of peritoneal fluid containing 1-2 X 10(6) macrophages/ml was available for each study. Macrophages made up 80-95% of the cells in the fluid. Fc and C3 ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.38.2.267
更新日期:1985-08-01 00:00:00
abstract::Flagellin, as a TLR5 agonist, is an established mucosal adjuvant for enhancing mucosal IgA responses by i.n. immunization. Nasal epithelial cells (NECs) are the first sentinel cells to be exposed to antigen and adjuvant in i.n. immunization, and it is suggested that they play an important role in the mucosal adjuvant ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3HI0816-368RR
更新日期:2017-09-01 00:00:00
abstract::The immunosuppressive activity of interleukin-10 (IL-10) makes this cytokine a potentially important clinical tool to reduce inflammatory responses in various diseases. Its efficacy as a therapeutic modality is dependent on the responsiveness of immune cells. We report that macrophages from mice chronically infected w...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract::Sepsis represents uncontrolled inflammation due to an infection. Cold-inducible RNA-binding protein (CIRP) is a stress-induced damage-associated molecular pattern (DAMP). A subset of neutrophils expressing ICAM-1+ neutrophils was previously shown to produce high levels of reactive oxygen species. The role of CIRP for ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3A0817-327RR
更新日期:2018-04-01 00:00:00
abstract::Intracellular zinc homeostasis is strictly regulated by zinc binding proteins and zinc transporters. In the present study, we quantified in a first global view the expression of all characterized human zinc exporters (hZnT-1-9) in different leukocyte subsets in response to zinc supplementation and depletion and analyz...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0307148
更新日期:2008-02-01 00:00:00
abstract::We have shown previously that PCNA, a nuclear factor involved in DNA replication and repair in proliferating cells, is localized exclusively in the cytoplasm of neutrophils, where it regulates their survival. Nuclear PCNA functions are tightly linked to its ring-shaped structure, which allows PCNA to bind to numerous ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1212637
更新日期:2013-10-01 00:00:00
abstract::Tumor-associated eosinophilia has been observed in numerous human cancers and several tumor models in animals; however, the details surrounding this eosinophilia remain largely undefined and anecdotal. We used a B16-F10 melanoma cell injection model to demonstrate that eosinophil infiltration of tumors occurred from t...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0106027
更新日期:2006-06-01 00:00:00
abstract::In recent years, it has become appreciated that immune cells have different metabolic profiles depending on their activation status. During sepsis, circulating leukocytes go through a hyperinflammatory state, which can be accompanied or followed by defective antimicrobial defenses (also described as immune tolerance o...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.4MR0216-066R
更新日期:2017-01-01 00:00:00
abstract::Extravasation of leukocytes is a crucial process in the immunological defense. In response to a local concentration of chemokines, circulating leukocytes adhere to and migrate across the vascular endothelium toward the inflamed tissue. The small guanosinetriphosphatase Rap1 plays an important role in the regulation of...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0506357
更新日期:2006-12-01 00:00:00
abstract::Severe injury can initiate an exaggerated systemic inflammatory response and multiple organ failure (MOF) if a subsequent immune stimulus, "second hit", occurs. Using a mouse thermal injury model, we tested whether changes in innate immune cell reactivity following injury can contribute to the development of heightene...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0704382
更新日期:2005-01-01 00:00:00
abstract::The CD2 receptor on T-lymphocytes plays a major part in mediating adhesive interactions via the LFA-3 ligand and in transducing signals for lymphocyte activation. In this study the expression, function, and internalization of the CD2 receptor was investigated in resting and activated murine T-cells. Surface iodination...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.49.4.329
更新日期:1991-04-01 00:00:00
abstract::Carcinoembryonic antigen (CEA), a member of the immunoglobulin supergene family, is recognized by a specific 80-kd receptor on Kupffer cells and alveolar macrophages. Peritoneal macrophages were harvested from male Sprague-Dawley rats 72 h after intraperitoneal injection with thioglycolate broth and from unstimulated ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.51.5.466
更新日期:1992-05-01 00:00:00
abstract::We tested several of the functions of macrophages (M phi) in the early phase after allogeneic bone marrow transfer to get information about this important aspect of the nonspecific immune system in the T-cell-deficient recipient. On days 3-5 after transfer, the number of M phi was reduced in the spleen, liver, lungs, ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.46.2.134
更新日期:1989-08-01 00:00:00
abstract::Increasing the dietary alpha-linolenate (18:3n - 3)/linoleate (18:2n - 6) ratio results in an increase in lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) production in mouse resident and casein-induced peritoneal macrophages [3]. We found that prostaglandin E2 (PGE2) production is inversely related to ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.53.2.151
更新日期:1993-02-01 00:00:00
abstract::Neutrophils phagocytize high-valency immune complexes (HIC) by an Fc gamma receptor-mediated mechanism, activating an oxidative burst and initiating degranulation. In contrast, neutrophils exhibit chemotaxis to N-formylated peptides [e.g., N-formyl-methionyl-leucyl-phenylalanine (fMLP)] and secrete far fewer oxidants ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.61.4.522
更新日期:1997-04-01 00:00:00
abstract::Eosinophil activation during allergic diseases has a detrimental role in the generation of pathophysiologic responses. Stem cell factor (SCF) has recently shown an inflammatory, gene-activating role on eosinophils and contributes to the generation of pathophysiologic changes in the airways during allergic responses. T...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0906595
更新日期:2007-04-01 00:00:00
abstract::Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial inf...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4A0118-030RR
更新日期:2018-12-01 00:00:00
abstract::Introduction of alloantigens into the AC induces a form of immune tolerance known as ACAID, which induces antigen-specific CD8+ Tregs, contributing to ocular immune privilege by down-regulating immune responses. Recent evidence suggests IFN-γ is needed for the suppressive function of CD8+ ACAID Tregs. This study teste...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0311173
更新日期:2012-03-01 00:00:00
abstract::The magnitude and efficacy of CD8(+) T cell memory may notably regress, especially if immune induction occurs in the absence of adequate CD4(+) help. This report demonstrates that this CD8(+) memory malfunction could be remedied if a source of cognate antigen-recognizing helper cells were provided during recall. The i...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0105007
更新日期:2005-10-01 00:00:00
abstract::In this study, we analyzed the specific effects of transforming growth factor beta (TGF-beta1) and/or IL-4 on monocyte-derived cells. Monocytes were cultured with GM-CSF, GM-CSF/TGF-beta1, GM-CSF/IL-4, or GM-CSF/IL-4/TGF-beta1 before cell morphology, phenotype, and function were assessed. As expected, interleukin-4 is...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Application of exogenous Abs targeting cell surface Ags has been widely used as an experimental approach to induce cell depletion or to inhibit receptor functionality. Moreover, Ab therapy is emerging as one of the mainstream strategies for cancer treatment. Previous studies on the mechanisms of Ab-mediated cell deple...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4A1219-078R
更新日期:2020-03-01 00:00:00
abstract::Previous studies about basophils and mast cells identified the ability of IgE to up-regulate FcepsilonRI expression by a process that depends on stabilization of the surface receptor by IgE. However, the mechanism of loss from the cell surface, when unoccupied, is not known. The current studies have examined whether u...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0207103
更新日期:2007-10-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) increase in tuberculosis (TB) and may be targets for host-directed therapy (HDT). In this study, we use flow cytometry to analyze the effects of cyclooxygenase-2 inhibitors (COX-2i) on monocytic (M)-MDSCs in blood from TB patients attending a clinical trial of COX-2i. The effec...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4A0720-409RR
更新日期:2020-11-06 00:00:00
abstract::In inflammatory diseases, circulating neutrophils are recruited to sites of injury. Attractant signals are provided by many different chemotactic molecules, such that blockade of one may not prevent neutrophil recruitment effectively. The Slit family of secreted proteins and their transmembrane receptor, Robo, repel a...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0609391
更新日期:2009-12-01 00:00:00
abstract::Cytosolic RIG-I-like helicases (RLR) are PRRs involved in type I IFN production and antiviral immunity. This study focuses to the comparison of the expression, function, and signaling cascades associated to RLR in the previously identified CD14(-)DC-SIGN(+)PPARγ(low)CD1a(+) and CD14(low)DC-SIGN(+)PPARγ(high)CD1a(-) hu...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0711360
更新日期:2012-07-01 00:00:00
abstract::Emerging evidence suggests that the innate immune system, comprised of Toll-like receptors (TLRs) and their associated molecules, plays a pivotal role in the regulation of intestinal inflammation and in the response to invading pathogens. Although TLRs are thought to have predominantly beneficial effects in pathogen r...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0607358
更新日期:2008-03-01 00:00:00
abstract::The effect of coupling C3bi to immunoglobulin G (IgG) immune complexes (IC) on their ability to produce protein tyrosine phosphorylation and activation of the mitogen-activated protein kinase (MAPK) and the Akt/protein kinase B (PKB) routes was assessed in human monocytes. Cross-linking Fc receptors for IgG activated ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1205701
更新日期:2006-05-01 00:00:00
abstract::We have characterized the beta-adrenergic receptor binding site and the beta-adrenergic cAMP response of the HL-60 cell. The hydrophilic ligand [3H]-(-)-CGP-12177 was specifically and reversibly bound to one single class of binding sites (Kd 220 pM and Bmax 1,970 sites/cell). The adrenergic agonists inhibited the spec...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.44.1.41
更新日期:1988-07-01 00:00:00