Abstract：
:A human breast cancer cell line (MCF-7) was used to develop an in vitro screening assay for the detection of xenoestrogenic environmental pollutants. MCF-7 cells were cultured in DMEM containing 5% fetal bovine serum (FBS). An estrogenic response was defined as an increase in the frequency of proliferating MCF-7 cells, and was measured using a thymidine analog, bromodeoxyuridine, and flow cytometry. Di-2-ethylhexyl phthalate (DEHP) and 4-n-nonylphenol (4-n-NP) were used as model chemicals. The proliferation rate of S-phase cells after 24 h of exposure to various concentrations of 17beta-estradiol and to model compounds was compared with a positive and a negative control, containing 1 nM 17beta-estradiol and 0.1% ethanol, respectively. DEHP and 4-n-NP increased the frequency of proliferating MCF-7 cells in a dose-dependent manner. The lowest concentration that significantly increased the proliferation of MCF-7 cells was 10 microM for DEHP and 1 microM for 4-n-NP. The results showed that the assay is accurate and quick to perform. It may prove a valuable tool for screening potential estrogen-mimicking environmental pollutants.
journal_name
Arch Environ Contam Toxicoljournal_title
Archives of environmental contamination and toxicologyauthors
Blom A,Ekman E,Johannisson A,Norrgren L,Pesonen Mdoi
10.1007/s002449900322subject
Has Abstractpub_date
1998-04-01 00:00:00pages
306-10issue
3eissn
0090-4341issn
1432-0703journal_volume
34pub_type
杂志文章abstract：:Using a conditioned proboscis extension response (PER) assay, honeybees (Apis mellifera L.) can be trained to associate an odor stimulus with a sucrose reward. Previous studies have shown that observations of conditioned PER were of interest for assessing the behavioral effects of pesticides on the honeybee. In the pr...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-003-0262-7
更新日期：2005-02-01 00:00:00
abstract：:Earlier incubation and greenhouse studies in our laboratory confirmed the effectiveness of drinking-water treatment residual (WTR) in decreasing soil arsenic (As) bioaccessibility as determined with in vitro tests, which led us to hypothesize a similar outcome if animal studies were to be conducted. Our objective was ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-009-9318-7
更新日期：2009-11-01 00:00:00
abstract：:An experimental model was designed to study the acute lesions caused by a continuous exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) disolved in water (400 mg/L) in hematopoietic kidney tissue in tench (Tinca tinca L). Fifty fish were used in this study, 15 for calculating LC50 and 35 were euthanized 1, 2, 5, 8, an...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s002449900405
更新日期：1998-10-01 00:00:00
abstract：:The chronic toxicity of total ammonium and unionized ammonia (NH3) to the native New Zealand freshwater fingernail clam Sphaerium novaezelandiae was assessed in soft water under laboratory conditions. Control survival after 60 days was high (93%) and concentration-response relationships showed the sensitivity of S. no...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s002449900440
更新日期：1999-01-01 00:00:00
abstract：:Selenium (Se) is an essential trace nutrient, but in excess, it can induce toxicity. Incomplete combustion of coal produces coal combustion wastes, which are enriched in Se and often disposed of in aquatic basins. While a multitude of studies have investigated Se accumulation in vertebrates, few studies have examined ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-017-0370-4
更新日期：2017-04-01 00:00:00
abstract：:We measured halogenated organic contaminants (HOCs) [polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), and dichloro-diphenyl-trichloroethane (DDT)] and P450 [e.g., ethoxyresorufin-O-dealkylase (EROD)] stress in livers from Caspian tern (Hydroprogne caspia) adults and Forster's tern (Sterna fors...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-009-9366-z
更新日期：2010-04-01 00:00:00
abstract：:The distribution of aliphatic and aromatic hydrocarbons in surface sediments of the lower course of the Imo River (Nigeria) was investigated to determine the sources and fate of these compounds. The aliphatic fraction is characterized by a widespread contribution of highly weathered/biodegraded hydrocarbon residues (r...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-015-0237-5
更新日期：2016-02-01 00:00:00
abstract：:Red-backed salamanders (Plethodon cinereus) were exposed to four different concentrations of 1,3,5-trinitrohexahydro-1,3,5-triazine (RDX) in soil under controlled laboratory conditions for 28 days. Wild-caught P. cinereus (N = 20/treatment) were exposed to target concentrations of 5,000, 1000, 100, 10, and 0 mg RDX/kg...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-004-3242-7
更新日期：2004-11-01 00:00:00
abstract：:The summer population of Mexican free-tailed bats (Tadarida brasiliensis) at Carlsbad Caverns, New Mexico, declined from an estimated 8.7 million in 1936 to 200,000 in 1974; thereafter, it increased to approximately 700,000 in 1991. This decline has been attributed primarily to organochlorine (OC) pesticide contaminat...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/BF00215796
更新日期：1996-02-01 00:00:00
abstract：:The toxicity of two amines, nonylamine and decylamine, which have a narcotic effect on organisms, and ethylparathion (enzymatic inhibitor) on Daphnia magna has been studied when acting singly and in joint toxicity tests. This was done on the basis of an isobologram method where curves of constant response, i.e., isobo...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-003-2179-6
更新日期：2003-10-01 00:00:00
abstract：:An acute exposure of two vanadate solutions-metavanadate and decavanadate-containing different vanadate oligomers, induces different patterns of subcellular vanadium distribution in blood plasma, red blood cells (RBC), and cardiac muscle subcellular fractions of the fish Sparus aurata (gilthead seabream). The highest ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-004-0246-2
更新日期：2006-01-01 00:00:00
abstract：:Mercury, cadmium, zinc, and copper concentrations were analyzed in three samples of common guillemot (in April, June, and November). Levels measured were uniformly low, and not enough to have any toxic effects. Adult guillemots had significantly more cadmium in their livers and kidneys than juveniles, with juvenile le...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/BF00214259
更新日期：1994-08-01 00:00:00
abstract：:A test system was developed to examine the effects of environmental contaminants on thiamine homeostasis in bird embryos. This system employs fresh chicken egg yolk lipids as a vehicle for use in egg injection studies. Furazolidone, an antibiotic suspected to interfere with thiamine metabolism, was used as a positive ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s002440010119
更新日期：2000-10-01 00:00:00
abstract：:The effect of growth and physical condition on the level of heavy metals accumulated in the organs of common bream (Abramis brama L.) populating Lake Balaton was investigated on samples collected in October 1999 and May 2000 from two well-separable sites regarding their trophic state and pollution impact (western and ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-002-1123-5
更新日期：2002-08-01 00:00:00
abstract：:Numerous studies have reported polychlorinated biphenyl (PCB) concentrations in soil, root, and aerial parts of vegetables. However, few studies have measured the contribution of PCBs bound to particles in air in relation to uptake by vegetables. In the present study, PCB concentrations were measured in five types of ...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-014-0045-3
更新日期：2014-07-01 00:00:00
abstract：:Acid rain is a serious environmental problem in the world and is of a particular concern in southern China where most of the soils are acidic. This study investigated the dynamics of cations, phosphorus (P), and soil organic matter (SOM) in the Latosol (acidic red soil) from south China under the influences of simulat...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-006-0004-8
更新日期：2007-01-01 00:00:00
abstract：:In many studies that investigate the toxic effects of pollutants on earthworms, experiments are performed using only one species of earthworms, most commonly the Eisenia species. However, the differences in sensitivities of different earthworm species could potentially lead to an underestimation of environmental aspec...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-013-9930-4
更新日期：2013-10-01 00:00:00
abstract：:Mercury is a heavy metal that has caused concern regarding the health of fish and of those who consume them. In this research, the concentrations of mercury in the muscle tissues of zander and European perch from Anzali were measured using a graphite furnace atomic absorption spectrometer (GFAAS). The average concentr...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-020-00730-1
更新日期：2020-07-01 00:00:00
abstract：:Exposures to airborne metals are known to cause physiological responses in organisms and wide-ranging health effects in humans. Hence determination of metals in particulate matter is important from a toxicological perspective. In the current study heavy metals associated with respirable (RSPM) and nonrespirable (NRSPM...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-004-3054-9
更新日期：2004-08-01 00:00:00
abstract：:Neonates from CD-1 mice, which were treated during gestation with 100 mg/kg of 2,4,5-T, were studied from day 1 to 30 postpartum (pp) for effects on cardiac development by determining total cardiac activities and isozyme profiles of LDH and CK. On day 1pp, the total activities and isozyme profiles of LDH of the neonat...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/BF01054881
更新日期：1981-09-01 00:00:00
abstract：:Selenium and mercury were evaluated in muscle, liver, kidney, brain, and the stomach contents of juvenile scalloped hammerhead shark Sphyrna lewini. Se:Hg molar ratios were calculated. The average Hg levels in muscle ranged from 0.12 to 1.17 μg/g (wet weight); Hg was <0.39 μg/g in liver and kidneys and <0.19 μg/g in b...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-015-0226-8
更新日期：2015-11-01 00:00:00
abstract：:The hooded crane is designated as an endangered species. The cranes breed primarily in wetlands in southeast Russia and China in summer. Most of the hooded crane population winters in the Izumi plain in Japan. It is difficult to know the contamination status of their habitat because of their vast breeding area. We det...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-018-0541-y
更新日期：2018-11-01 00:00:00
abstract：:Nitrous oxide (N2O) emissions from a rice-duck cultivation system in the subtropical region of China and its regulating factors were investigated by using a static chambers technique during rice growth seasons in 2006 and 2007. The experimental field was equally divided into six plots for two different treatments: One...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-008-9159-9
更新日期：2009-01-01 00:00:00
abstract：:Although Antarctica is considered one of the most pristine areas on Earth, an accelerating human presence in this remote continent, such as scientific operations and functioning of numerous scientific stations, logistics, and tourism activities, has increased the risks of environmental impacts in recent decades. Durin...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-021-00808-4
更新日期：2021-01-21 00:00:00
abstract：:Channel catfish (Ictalarus punctatus) were exposed to 0, 10, 20, and 40% bleached kraft pulp and paper mill effluent (BKME) for up to two weeks. Fish were sacrificed on days 1, 3, 7, and 14 of exposure. Blood, liver, and gill tissues were assayed for biochemical responses associated with oxidative stress (superoxide d...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/BF01064409
更新日期：1991-04-01 00:00:00
abstract：:Herbicides, such as atrazine, are extensively used in agriculture in order to suppress growth of weeds. From the soil they inevitably find their way to water supplies, leading to human exposure via drinking water. Therefore, it is extremely important to know whether those chemicals pose any hazard to public health. Th...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-003-3075-9
更新日期：2004-04-01 00:00:00
abstract：:Effect of sublethal treatment (20% and 60% of LC(50)/24 h) of plant-derived molluscicides, viz. Polianthes tuberosa, Trachyspermum ammi, Allium sativum powder; Azadirachta indica oil; oleoresin of Zingiber officinale; and their active molluscicidal component in combination (1:5) with MGK-264 or piperonyl butoxide on t...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s002449910024
更新日期：2000-02-01 00:00:00
abstract：:Static acute toxicity bioassays were conducted at 20 degrees C and 20 o/33 salinity with CdCl2-2 1/2 H20, K2CrO4, HgCl2, NiCl2-6H2O, and ZnCl2 using adults of starfish, Asterias forbesi; sandworm, Nereis virens; hermit crab, Pagurus longicarpus; softshell clam, Mya arenaria; mudsnail, Nassarius obsoletus; and mummicho...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/BF02097772
更新日期：1977-01-01 00:00:00
abstract：:The surficial sediments were collected in winter and summer (2015) from the coastal areas of Bangladesh and analyzed for 16 USEPA priority polycyclic aromatic hydrocarbons (PAHs). The total concentration of PAHs (∑PAHs) were 349.8-11,058.8 and 199.9-17,089.1 ng/g dry weight (dw) in winter and summer, respectively. Sed...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-018-0571-5
更新日期：2019-02-01 00:00:00
abstract：:The eastern North American population of Barrow's goldeneyes winters in the St. Lawrence Estuary and Gulf of St. Lawrence where the sediments and food web are known to be contaminated with inorganic and organic compounds. Therefore, there is a potential for contamination of this population, which is designated of Spec...
journal_title：Archives of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00244-012-9787-y
更新日期：2012-10-01 00:00:00
