Abstract:
:In fixed tissue, neuronal NADPH-diaphorase staining results from nitric oxide synthase (NOS) activity. Neuronal NOS only synthesizes nitric oxide once activated by the binding of Ca2+/calmodulin. We show here that neuronal NADPH-diaphorase staining is also dependent on Ca2+/calmodulin, implying that only activated NOS is detected. In addition, in bovine pulmonary endothelial cells, carbachol and bradykinin dramatically and rapidly increase the intensity of NADPH-diaphorase staining. Furthermore, administration of MK801, an NMDA antagonist, decreases neuronal NADPH-diaphorase staining. This suggests that the intensity of the NADPH-diaphorase staining is related to the level of enzyme activation at the moment of tissue fixation. The potential of exploiting this observation to detect cellular activation of NOS is illustrated by the observations that the intensity of NADPH-diaphorase staining in rat striatal neurones is decreased following systemic treatment with the D1-like dopamine receptor antagonist SCH23390, and increased by the D2-like antagonist eticlopride. These results therefore provide strong evidence that the NADPH-diaphorase reaction can be used to monitor NOS activity at a cellular level of resolution, and reveal a dopaminergic regulation of NOS activity in the striatum mediated by D1-like and D2-like dopamine receptors.
journal_name
Neuropharmacologyjournal_title
Neuropharmacologyauthors
Morris BJ,Simpson CS,Mundell S,Maceachern K,Johnston HM,Nolan AMdoi
10.1016/s0028-3908(97)00159-7subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1589-99issue
11-12eissn
0028-3908issn
1873-7064journal_volume
36pub_type
杂志文章abstract::The group II metabotropic glutamate (mGlu) receptors comprised of the mGlu2 and mGlu3 receptor subtypes have gained recognition in recent years as potential targets for psychiatric disorders, including anxiety and schizophrenia. In addition to studies already indicating which subtype mediates the anxiolytic and anti-p...
journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
pub_type: 杂志文章,评审
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更新日期:2017-09-15 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(85)90062-0
更新日期:1985-06-01 00:00:00
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journal_title:Neuropharmacology
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更新日期:2016-10-01 00:00:00
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journal_title:Neuropharmacology
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doi:10.1016/j.neuropharm.2014.02.019
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pub_type: 杂志文章
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更新日期:2000-08-23 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2013.05.034
更新日期:2013-10-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
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更新日期:2017-01-01 00:00:00
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journal_title:Neuropharmacology
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更新日期:2011-12-01 00:00:00
abstract::Repeated stress exposure can lead to the development of anxiety and mood disorders. An emerging biological substrate of depression and associated pathology is the nucleus accumbens (NAc), which through interactions with limbic, cognitive and motor circuits can regulate a variety of stress responses. Within these circu...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2017.07.008
更新日期:2017-10-01 00:00:00
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journal_title:Neuropharmacology
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更新日期:2007-03-01 00:00:00
abstract::The kinetics of synaptic currents is largely determined by the postsynaptic receptor gating and the concentration time course of synaptic neurotransmitter. While the analysis of current responses to rapid agonist application provides the means to study the ligand-gated receptor gating, no direct tools are available to...
journal_title:Neuropharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.neuropharm.2004.07.003
更新日期:2004-12-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2014.07.001
更新日期:2014-11-01 00:00:00
abstract::NMDA receptors have been known to play a central role in long-term potentiation at glutamatergic synapses in principal cells for thirty years. In contrast, their roles in the development and activity-dependent plasticity of synapses in inhibitory circuits have only recently begun to be understood. Progress has, to a g...
journal_title:Neuropharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.neuropharm.2013.03.004
更新日期:2013-11-01 00:00:00