Development of stable film test for rapid estimation of blood or plasma 3-hydroxybutyrate.

Abstract:

:A film test for the rapid detection of plasma/serum 3-hydroxybutyrate (3-OHB) has been developed. The film contains NAD, nitro blue tetrazolium, 3-OHB dehydrogenase, and diaphorase, and the surface is coated with modified biomembrane and can detect 50-1500 microM 3-OHB within 2-3 min. One drop or 50 microliters of plasma/serum or blood is applied to the film, and the violet color is read via reflectance meter after 2 min. Plasma/serum samples greater than 1500 microM 3-OHB can be measured by dilution with saline. In blood with 40% hematocrit, the color developed is 50% less than with plasma/serum, and this was adjusted in the reflectance meter. A good correlation (r = 0.99) was observed between results with automated and film methods and between visual methods and reflectance meter. In insulin-dependent diabetes mellitus, all 3 subjects with positive ketonuria (+ +), 8 of 12 subjects with mild ketonuria (+), and 7 of 25 subjects without ketonuria exhibited elevation of 3-OHB in blood greater than 200 microM. The results indicate that 3-OHB film is valuable not only in the emergency room for the differential diagnosis between ketoacidotic and nonketotic hypersomolar coma but also as a marker for insulin dependency, energy dependency on fatty acid compared with glucose, and metabolic control of diabetes.

journal_name

Diabetes Care

journal_title

Diabetes care

authors

Harano Y,Aoki T,Nakajima Y,Kojima H,Kosugi K,Harano A,Hidaka H,Yamada S,Uno S,Shigeta Y

doi

10.2337/diacare.13.5.522

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

522-4

issue

5

eissn

0149-5992

issn

1935-5548

journal_volume

13

pub_type

杂志文章
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    authors: Raskin P,Klaff L,Bergenstal R,Hallé JP,Donley D,Mecca T

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    pub_type: 杂志文章

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    authors: Vetter C,Dashti HS,Lane JM,Anderson SG,Schernhammer ES,Rutter MK,Saxena R,Scheer FAJL

    更新日期:2018-04-01 00:00:00

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    journal_title:Diabetes care

    pub_type: 杂志文章,评审

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    authors: Sorkin JD,Muller DC,Fleg JL,Andres R

    更新日期:2005-11-01 00:00:00

  • Mortality and morbidity associated with body weight in people with IDDM. The WHO Multinational Study of Vascular Disease in Diabetes.

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    journal_title:Diabetes care

    pub_type: 杂志文章,多中心研究

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    authors: Chaturvedi N,Stevens LK,Fuller JH

    更新日期:1995-06-01 00:00:00

  • Comparison of glargine insulin versus rosiglitazone addition in poorly controlled type 2 diabetic patients on metformin plus sulfonylurea.

    abstract:OBJECTIVE:We sought to examine the mechanisms by which the addition of glargine insulin or rosiglitazone improves glycemic control in type 2 diabetic subjects poorly controlled on maximally effective doses of metformin plus sulfonylurea. RESEARCH DESIGN AND METHODS:Subjects (aged 47 +/- 11 years, BMI 31 +/- 5 kg/m(2),...

    journal_title:Diabetes care

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  • Novel use and utility of integrated electronic health records to assess rates of prediabetes recognition and treatment: brief report from an integrated electronic health records pilot study.

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    journal_title:Diabetes care

    pub_type: 杂志文章

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    authors: Schmittdiel JA,Adams SR,Segal J,Griffin MR,Roumie CL,Ohnsorg K,Grant RW,O'Connor PJ

    更新日期:2014-02-01 00:00:00

  • Association between renal failure and foot ulcer or lower-extremity amputation in patients with diabetes.

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    journal_title:Diabetes care

    pub_type: 杂志文章,多中心研究

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    authors: Margolis DJ,Hofstad O,Feldman HI

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    journal_title:Diabetes care

    pub_type: 临床试验,杂志文章,随机对照试验

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    pub_type: 杂志文章

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    journal_title:Diabetes care

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  • Vital signs, QT prolongation, and newly diagnosed cardiovascular disease during severe hypoglycemia in type 1 and type 2 diabetic patients.

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    journal_title:Diabetes care

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  • Temporal validation of the UKPDS outcomes model using 10-year posttrial monitoring data.

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    journal_title:Diabetes care

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  • A Multidisciplinary Evaluation of a Virtually Supervised Home-Based High-Intensity Interval Training Intervention in People With Type 1 Diabetes.

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    journal_title:Diabetes care

    pub_type: 杂志文章

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  • Deranged platelet calcium homeostasis in diabetic patients with end-stage renal failure. A possible link to increased cardiovascular mortality?

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    journal_title:Diabetes care

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  • Diabetes, inflammation, and functional decline in older adults: findings from the Health, Aging and Body Composition (ABC) study.

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    journal_title:Diabetes care

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  • Diminished thyroxine-binding globulin in pubertal diabetic children.

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    journal_title:Diabetes care

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    doi:10.2337/diacare.19.3.246

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    journal_title:Diabetes care

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.2337/dc14-2101

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  • Distinct Patterns of Daily Glucose Variability by Pubertal Status in Youth With Type 1 Diabetes.

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    pub_type: 评论,杂志文章

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    pub_type: 评论,杂志文章

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